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pCAhIL12p40

Expression vector of human IL-12 p40 cDNA

Catalog number RDB03959
Resource name pCAhIL12p40
Alternative name pCAcchIL12p40
Clone info. Expression vector of human IL12p40 driven by CA promoter. cDNA fragment 43nt to 1029nt of NM_002187.2 is cloned.
Expression was confirmed with ELISA by depositor.
Known difference (at least): C to G substitution at 381nt (Ser, synonym).
Vector backbone pCA (Plasmid)
Selectable markers Amp^r
Gene/insert name Human interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human IL12B (NCBI Gene 3593) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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RDB03959 pCAhIL12p40 DNA solution

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15737_A8Ahp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 RDB15737_A8Aha.seq
>03959_15737_A8Ah_pCAG-F_V2_E01_05_ABI08.ab1
    1 GCGGATGGTT GTTTCAATCT GGTGCGGAGC CGAATCTGGG AGGCGCCGCC GCACCCCCTC
   61 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT
  121 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG CCTCGGGGCT GTCCGCGGGG
  181 GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC GTGTGACCGG
  241 CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG
  301 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCTAGA CTGCCATGTG
  361 TCACCAGCAG TTGGTCATCT CTTGGTTTTC CCTGGTTTTT CTGGCATCTC CCCTCGTGGC
  421 CATATGGGAA CTGAAGAAAG ATGTTTATGT CGTAGAATTG GATTGGTATC CGGATGCCCC
  481 TGGAGAAATG GTGGTCCTCA CCTGTGACAC CCCTGAAGAA GATGGTATCA CCTGGACCTT
  541 GGACCAGAGC AGTGAGGTCT TAGGCTCTGG CAAAACCCTG ACCATCCAAG TCAAAGAGTT
  601 TGGAGATGCT GGCCAGTACA CCTGTCACAA AGGAGGCGAG GTTCTAAGCC ATTCGCTCCT
  661 GCTGCTTCAC AAAAAGGAAG ATGGAATTTG GTCGACTGAT ATTTTAAAGG ACCAGAAAGA
  721 ACCCAAAAAT AAGACCTTTC TAAGATGCGA GGCCAAGAAT TATTCTGGAC GTTTCACCTG
  781 CTGGTGGCTG ACGACAATCA GTACTGATTT GACATTCAGT GTCAAAAGCA GCAGAGGCTC
  841 TTCTGACCCC CAAGGGGTGA CGTGCGGAGC TGCTACACTC TCTGCAGAGA GAGTCAGAGG
  901 GGACAACAAG GAGTATGAGT ACTCAGTGGA GTGCCAGGAG GACAGTGCCT GCCCAGCTGC
  961 TGAGGAGAGT CTGCCCATTG AGGTCATGGT GGATGCCGTT CACAAGCTCA AGTATGAAAA
 1021 CTACACCAGC AGCTTCTTCA TCAGGACATC ATCAAACCTG ACCCACCCAG ACTTGCAGCT
 1081 GAGCCATTAA AGAATTCTCG CAGGTGAGTC AGCTGGGGAG TACTGGACAC TTGAAGTACT
1141 TCACATTCCT TACCTTCTCC TTGACACTTC TGCGTTCAGG GTTCCAAGG
//
Primer: pCA-bgter RDB15737_A8Ahb.seq
>03959_15737_A8Ah_pCA-bgter_F01_06_ABI08.ab1
    1 AACCCGGAAG GTCCCATAAT TTTTGGCAGA GGGAAAAGAT CTCAGTGGTA TTTGTGAGCC
   61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT
  121 TCTAGAGGAT CGATCCTAAC TGCAGGGCAC AGATGCCCAT TCGCTCCAAG ATGAGCTATA
  181 GTAGCGGTCC TGGGCCCGCA CGCTAATGCT GGCATTTTTG CGGCAGATGA CCGTGGCTGA
  241 GGTCTTGTCC GTGAAGACTC TATCTTTCTT TTCTCTCTTG CTCTTGCCCT GGACCTGAAC
  301 GCAGAATGTC AGGGAGAAGT AGGAATGTGG AGTACTCCAG GTGTCAGGGT ACTCCCAGCT
  361 GACCTCCACC TGCCGAGAAT TCTTTAATGG CTTCAGCTGC AAGTTCTTGG GTGGGTCAGG
  421 TTTGATGATG TCCCTGATGA AGAAGCTGCT GGTGTAGTTT TCATACTTGA GCTTGTGAAC
  481 GGCATCCACC ATGACCTCAA TGGGCAGACT CTCCTCAGCA GCTGGGCAGG CACTGTCCTC
  541 CTGGCACTCC ACTGAGTACT CATACTCCTT GTTGTCCCCT CTGACTCTCT CTGCAGAGAG
  601 TGTAGCAGCT CCGCACGTCA CCCCTTGGGG GTCAGAAGAG CCTCTGCTGC TTTTGACACT
  661 GAATGTCAAA TCAGTACTGA TTGTCGTCAG CCACCAGCAG GTGAAACGTC CAGAATAATT
  721 CTTGGCCTCG CATCTTAGAA AGGTCTTATT TTTGGGTTCT TTCTGGTCCT TTAAAATATC
  781 AGTCGACCAA ATTCCATCTT CCTTTTTGTG AAGCAGCAGG AGCGAATGGC TTAGAACCTC
  841 GCCTCCTTTG TGACAGGTGT ACTGGCCAGC ATCTCCAAAC TCTTTGACTT GGATGGTCAG
  901 GGTTTTGCCA GAGCCTAAGA CCTCACTGCT CTGGTCCAAG GTCCAGGTGA TACCATCTTC
  961 TTCAGGGGTG TCACAGGTGA GGACCACCAT TTCTCCAGGG GCATCCGGAT ACCAATCCCA
 1021 ATTCTACGAC ATAAACATCT TTCTTCAGTT CCCATATGGG CCACGAGGGG AGATGCCAGA
 1081 AAAACAGGGA AAACCAGGAG ATGACCAACT GCTGTGACAC ATGGCAGGTC TAGGAGTTCT
 1141 TGCCCAAAAT GATGAAGACA GCCACATTAC TAGCACGTTG GCCAGAGCGT TAGGAAGGTA
1201 GAGACCATGT GTA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.12.10

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