RDB03959 - pCAhIL12p40

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pCAhIL12p40 (#RDB03959)

Expression vector of human IL-12 p40 cDNA

Alternative name	pCAcchIL12p40
Clone info.	Expression vector of human IL12p40 driven by CA promoter. cDNA fragment 43nt to 1029nt of NM_002187.2 is cloned.
Comment	Expression was confirmed with ELISA by depositor. Known difference (at least): C to G substitution at 381nt (Ser, synonym).
Vector backbone	pCA (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) cDNA
Depositor\|Developer	Hamada, Hirofumi \|

Distribution information

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB03959	pCAhIL12p40	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAhIL12p40 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03959).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15737_A8Ahp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V2 Sequence file: RDB15737_A8Aha.seq
>03959_15737_A8Ah_pCAG-F_V2_E01_05_ABI08.ab1 1 GCGGATGGTT GTTTCAATCT GGTGCGGAGC CGAATCTGGG AGGCGCCGCC GCACCCCCTC 61 TAGCGGGCGC GGGGCGAAGC GGTGCGGCGC CGGCAGGAAG GAAATGGGCG GGGAGGGCCT 121 TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT CCCTCTCCAG CCTCGGGGCT GTCCGCGGGG 181 GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC GTGTGACCGG 241 CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG 301 CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCTAGA CTGCCATGTG 361 TCACCAGCAG TTGGTCATCT CTTGGTTTTC CCTGGTTTTT CTGGCATCTC CCCTCGTGGC 421 CATATGGGAA CTGAAGAAAG ATGTTTATGT CGTAGAATTG GATTGGTATC CGGATGCCCC 481 TGGAGAAATG GTGGTCCTCA CCTGTGACAC CCCTGAAGAA GATGGTATCA CCTGGACCTT 541 GGACCAGAGC AGTGAGGTCT TAGGCTCTGG CAAAACCCTG ACCATCCAAG TCAAAGAGTT 601 TGGAGATGCT GGCCAGTACA CCTGTCACAA AGGAGGCGAG GTTCTAAGCC ATTCGCTCCT 661 GCTGCTTCAC AAAAAGGAAG ATGGAATTTG GTCGACTGAT ATTTTAAAGG ACCAGAAAGA 721 ACCCAAAAAT AAGACCTTTC TAAGATGCGA GGCCAAGAAT TATTCTGGAC GTTTCACCTG 781 CTGGTGGCTG ACGACAATCA GTACTGATTT GACATTCAGT GTCAAAAGCA GCAGAGGCTC 841 TTCTGACCCC CAAGGGGTGA CGTGCGGAGC TGCTACACTC TCTGCAGAGA GAGTCAGAGG 901 GGACAACAAG GAGTATGAGT ACTCAGTGGA GTGCCAGGAG GACAGTGCCT GCCCAGCTGC 961 TGAGGAGAGT CTGCCCATTG AGGTCATGGT GGATGCCGTT CACAAGCTCA AGTATGAAAA 1021 CTACACCAGC AGCTTCTTCA TCAGGACATC ATCAAACCTG ACCCACCCAG ACTTGCAGCT 1081 GAGCCATTAA AGAATTCTCG CAGGTGAGTC AGCTGGGGAG TACTGGACAC TTGAAGTACT 1141 TCACATTCCT TACCTTCTCC TTGACACTTC TGCGTTCAGG GTTCCAAGG //
Primer: pCA-bgter Sequence file: RDB15737_A8Ahb.seq
>03959_15737_A8Ah_pCA-bgter_F01_06_ABI08.ab1 1 AACCCGGAAG GTCCCATAAT TTTTGGCAGA GGGAAAAGAT CTCAGTGGTA TTTGTGAGCC 61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT 121 TCTAGAGGAT CGATCCTAAC TGCAGGGCAC AGATGCCCAT TCGCTCCAAG ATGAGCTATA 181 GTAGCGGTCC TGGGCCCGCA CGCTAATGCT GGCATTTTTG CGGCAGATGA CCGTGGCTGA 241 GGTCTTGTCC GTGAAGACTC TATCTTTCTT TTCTCTCTTG CTCTTGCCCT GGACCTGAAC 301 GCAGAATGTC AGGGAGAAGT AGGAATGTGG AGTACTCCAG GTGTCAGGGT ACTCCCAGCT 361 GACCTCCACC TGCCGAGAAT TCTTTAATGG CTTCAGCTGC AAGTTCTTGG GTGGGTCAGG 421 TTTGATGATG TCCCTGATGA AGAAGCTGCT GGTGTAGTTT TCATACTTGA GCTTGTGAAC 481 GGCATCCACC ATGACCTCAA TGGGCAGACT CTCCTCAGCA GCTGGGCAGG CACTGTCCTC 541 CTGGCACTCC ACTGAGTACT CATACTCCTT GTTGTCCCCT CTGACTCTCT CTGCAGAGAG 601 TGTAGCAGCT CCGCACGTCA CCCCTTGGGG GTCAGAAGAG CCTCTGCTGC TTTTGACACT 661 GAATGTCAAA TCAGTACTGA TTGTCGTCAG CCACCAGCAG GTGAAACGTC CAGAATAATT 721 CTTGGCCTCG CATCTTAGAA AGGTCTTATT TTTGGGTTCT TTCTGGTCCT TTAAAATATC 781 AGTCGACCAA ATTCCATCTT CCTTTTTGTG AAGCAGCAGG AGCGAATGGC TTAGAACCTC 841 GCCTCCTTTG TGACAGGTGT ACTGGCCAGC ATCTCCAAAC TCTTTGACTT GGATGGTCAG 901 GGTTTTGCCA GAGCCTAAGA CCTCACTGCT CTGGTCCAAG GTCCAGGTGA TACCATCTTC 961 TTCAGGGGTG TCACAGGTGA GGACCACCAT TTCTCCAGGG GCATCCGGAT ACCAATCCCA 1021 ATTCTACGAC ATAAACATCT TTCTTCAGTT CCCATATGGG CCACGAGGGG AGATGCCAGA 1081 AAAACAGGGA AAACCAGGAG ATGACCAACT GCTGTGACAC ATGGCAGGTC TAGGAGTTCT 1141 TGCCCAAAAT GATGAAGACA GCCACATTAC TAGCACGTTG GCCAGAGCGT TAGGAAGGTA 1201 GAGACCATGT GTA //