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p3mutant CRE RE-TK hRluc(F) (#RDB03460)

Reporter construct of CRE response element/PMA

Alternative name 2060C164F
Clone info. mutant 3' CRE is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the mutant 3' CRE is 5'-GATCCTGCAGTCATGCAGTCATGCAGTCAG-3'.
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial CRE response element/PMA, mutated synthesized
Depositor|Developer Yokoyama, Kazunari |
 
Remarks, protocol and/or map (pdf) RDB03460.pdf

Distribution information

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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB03460 p3mutant CRE RE-TK hRluc(F) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3mutant CRE RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03460).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13700_A5Jtp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: TKproR
Sequence file: RDB13700_A5Jta.seq check
>03460_13700_A5Jt_TKpro_R_G03_21.ab1
    1 CCTAGTAAGT GTTTCGTTCT TCCCCTCCCC CCGCGTCAGA CAAACCCTAA CCACCGCTTA
   61 AGCGGCCCCC GCGAGGTCCG AAGACTCATT TAGATCCTGA CTGCATGACT GCATGACTGC
  121 AGGATCTGTC GAGCCATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
  181 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
  241 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
  301 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
  361 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
  421 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
  481 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
  541 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
  601 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG
  661 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
  721 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
  781 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
  841 TAAGGGATTT TGGTCATGAG ATTATCAAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
  901 AAAATGAAGT TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGGTC TGACAGTTTA
  961 CCAAATGCTT AATCAGTGAG GCACCCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
 1021 GTGCCTTGAC TTCCCCCGTT CGTGTTAGAA TACTAACTAT ACCGGCAAGG ACCTAACCAT
1081 CTGGACCCCA GTGCCTTGCC GAG
//
Primer: pAxCALNL_F1
Sequence file: RDB13700_A5Jtb.seq check
>03460_13700_A5Jt_pAxCALNL_F1_H03_24.ab1
    1 AAAGTATCTT ATCATGTCTG CTCGAGCGGC CGCTCTAGAA TTACTGCTCG TTCTTCAGCA
   61 CGCGCTCCAC GAAGCTCTTG ATGTACTTAC CCATTTCATC TGGAGCGTCC TCCTGGCTGA
  121 AGTGGAGGCC CTTCACCTTC ACGAACTCGG TGTTAGGGAA CTTCTTAGCT CCCTCGACAA
  181 TAGCGTTGGA AAAGAACCCA GGGTCGGACT CGATGAACAT CTTAGGCAGA TCGTCGCTGG
  241 CCCGAAGGTA GGCGTTGTAG TTGCGGACAA TCTGGACGAC GTCGGGCTTG CCTCCCTTAA
  301 CGAGAGGGAT CTCGCGAGGC CAGGAGAGGG TAGGCCGTCT AACCTCGCCC TTCTCCTTGA
  361 ATGGCTCCAG GTAGGCAGCG AACTCCTCAG GCTCCAGTTT CCGCATGATC TTGCTTGGGA
  421 GCATGGTCTC GACGAAGAAG TTATTCTCAA GCACCATTTT CTCGCCCTCT TCGCTCTTGA
  481 TCAGGGCGAT ATCCTCCTCG ATGTCAGGCC ACTCGTCCCA GGACTCGATC ACGTCCACGA
  541 CACTCTCAGC ATGGACGATG GCCTTGATCT TGTCTTGGTG CTCGTAGGAG TAGTGAAAGG
  601 CCAGACAAGC CCCCCAGTCG TGGCCCACAA AGATGATTTT CTTTGGAAGG TTCAGCAGCT
  661 CGAACCAAGC GGTGAGGTAC TTGTAGTGAT CCAGGAGGCG ATATGAGCCA TTCCCGCTCT
  721 TGCCGGACTT ACCCATTCCG ATCAGATCAG GGATGATGCA TCTAGCCACG GGCTCGATGT
  781 GAGGCACGAC GTGCCTCCAC AGGTAGCTGG AGGCAGCGTT ACCATGCAGA AAAATCACGG
  841 CGTTCTCGGC GTGCTTCTCG GAATCATAGT AGTTGATGAA GGAGTCCAGC ACGTTCATTT
  901 GCTTGCAGCG AGCCCACCAC TGAGGCCCAG TGATCATGCG TTTGCGTTGC TCGGGGTCGT
  961 ACACCCTTGG AAAGCCATGG GTGGGCTAGC CCTATAGTGA GTCGTTATTT AAGTTACTCT
 1021 AGCCCTTTAG AGCTGGTAGT GGAACTTGCG CAGGTGGACA CTGATGCAGA GAAAGTAAAG
1081 TGGCATTGTT CCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles