Please review the QC test results indicated by icon below as well as clone information before placing your order.

phB2Mi_i_DsRed2

Expression vector of human microglobulin

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備考	クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19168_B1F4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-R (Pr0037) Region: IRES2,insert,CMV pro Sequence file: RDB19168_B1F4a.seq
>03438_19168_B1F4_3_IRES_R_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTAG GGGGGGGGGA NGGNNNNGGG CGGATCCCAT    61 CCTCGAGTTA CATGTCTCGA TCCCACTTAA CTATCTTGGG CTGTGACAAA GTCACATGGT   121 TCACACGGCA GGCATACTCA TCTTTTTCAG TGGGGGTGAA TTCAGTGTAG TACAAGAGAT   181 AGAAAGACCA GTCCTTGCTG AAAGACAAGT CTGAATGCTC CACTTTTTCA ATTCTCTCTC   241 CATTCTTCAG TAAGTCAACT TCAATGTCGG ATGGATGAAA CCCAGACACA TAGCAATTCA   301 GGAAATTTGA CTTTCCATTC TCTGCTGGAT GACGTGAGTA AACCTGAATC TTTGGAGTAC   361 GCTGGATAGC CTCCAGGCCA GAAAGAGAGA GTAGCGCGAG CACAGCTAAG GCCACGGAGC   421 GAGACATCTC GGATCCGATG GGCCCGCGGT ACCGTCGACT GCAGAATTCG AAGCTTGAGC   481 TCGAGATCTG AGTCCGGTAG CGCTAGCGGA TCTGACGGTT CACTAAACCA GCTCTGCTTA   541 TATAGACCTC CCACCGTACA CGCCTACCGC CCATTTGCGT CNATGGGGCG GANTTGTTAC   601 GACNTTTTGG AAAGTCCCNT TGANTTTGNN GNCNAAANAA ACTCCCATTG ACGTCANTGG   661 GGGNGGANAC TTNNNAANCC CGNGANTCNN NCNNCTATNN NCNNCNNNTN GNNGNNCTNN 721 CNAAAANCNN CATNNNNNNN NGNNNNNNNN NN //
Primer: IRES-F (Pr0036) Region: IRES2,DsRed2 5' Sequence file: RDB19168_B1F4b.seq
>03438_19168_B1F4_3_IRES_F_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNANGNN NNNNAACGTC TAGGCCCCCC GAACCACGGG    61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG CCTCCTCCGA   121 GAACGTCATC ACCGAGTTCA TGCGCTTCAA GGTGCGCATG GAGGGCACCG TGAACGGCCA   181 CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCCACA ACACCGTGAA   241 GCTGAAGGTG ACCAAGGGCG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCCCAGTT   301 CCAGTACGGC TCCAAGGTGT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACAAGAAGCT   361 GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGC   421 GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCTGCTTC ATCTACAAGG TGAAGTTCAT   481 CGGCGTGAAC TTCCCCTCCG ACGGCCCCGT GATGCAGAAG AAGACCATGG GCTGGGANGC   541 CTCCACCGAG CGCCTGTACC CCCGCGACGG CGTGCTGAAN GGCGANACCC ACAAGGCCCT   601 GAAGCTGAAG GACGGNNGCC ACTACCTGGN GGAGTTNNAG TCTATCTACA TGGNNNAGAA   661 GCCCGTGCAG CTGCCCNGNT ACTACTACNN NNNNGCNNNG CTGGANNTCN NNTNCCNNNN 721 NNNNGNACNA NNNCNNNNNN NNNN //
Primer: mRFP1-C_F (Pr0502) Region: DsRed2 3',SV40 pA,f1 ori Sequence file: RDB19168_B1F4c.seq
>03438_19168_B1F4_3mRFP1-C_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GNNNTCACCT CCCACAACGA GGNNTACACC ATCGTGGAGC    61 AGTACGAGCG CACCGNGGGC CGCCACCACC TGTTCCTGTA GCGGCCGCGA CTCTAGATCA   121 TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC   181 CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT   241 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC   301 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGGCGTA AATTGTAAGC   361 GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA   421 TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT   481 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG   541 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT   601 TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AANGGAGCCC CCGANTTANA 661 GCNTGACGGG GNAAGCCGGC NNNNGTNGNN ANNAANGNNN NNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB19168_B1F4d.seq
>03438_19168_B1F4_3_SV40pro_ori_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNGCCGCCT CNGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT    61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA   121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT   181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC   241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG   301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG   361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC   421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC   481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG   541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGANGATCTG GACGAAGAGC   601 ATCANGGGCT CGCGCCNGCC GAACTGTTCG CCAGGCTCNA GGCGAGCATG CCCNACNGCN   661 ANGNNCTCGT CGTGNNCCAT NNNNANGNNN GNNNGNNGAA TANCNTNNNN GNAAAATNNN 721 CNNCTTTTCN GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.12.12

GNP_filter3_RDBDEP_html_231212.pl