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pUC18-HTK (with promoter)

Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene

Catalog number RDB03436
Resource name pUC18-HTK (with promoter)
Clone info. Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene.
Vector backbone pUC18 (Plasmid)
Gene/insert name Staphylococcus aureus kanamycin nucleotidyltransferase Genomic DNA
Reference of nucleotide sequence AB121443 (DDBJ accession number)
Depositor Hoseki, Jun |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Biochem. (Tokyo)., 126, 951-956, 1999)
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RDB03436 pUC18-HTK (with promoter) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB03436.pdf from Depositor

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References

original Hoseki, J., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13745_A7Kap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13745_A7Kaa.seq
>03436_13745_A7Ka_M13_-40_D03_04_ABI08.ab1
    1 AGGCAGAGGG CAAGCCTTTG CATGTCTGCA GCGTAACCAA CATGATTAAC AATTATTAGA
   61 GGTCATCGTT CAAAATGGTA TGCGTTTTGA CACATCCACT ATATATCCGT GTCGTTCCGC
  121 CCACTCCTGA ACCCCATTCC AGAAATTCTC TAGCGATTCC AGAAGTTTCT CAGGGTCGGA
  181 AAGTTGACCA GACATTACGA GCTGGCACAG TTGGACATAA CCTGGAGGAA GATCTGGTTG
  241 CTTAACTGCT TCAGTTAAGA CCGAAGCGCT CGTCGTATAA CAGATGCGAT GATGCAGACC
  301 AATCAACATG GCACCTGCCA TTGCCACCTG TACAGTCAAG GATGGTAGAA ATGTTGTCGG
  361 TCCTTGCACA CGAATATTAC GCCATTTGCC TGCATATTCA AACAGCTCTT CTACGATAAG
  421 GGCACAGATC GCATCGTGGA ACTTTTGGGC TTCTACCGAT TTAGCAGTTT GGTACACTTT
  481 CTCAAAGTAT CCACCTGGAT CATAAATCGG CAAAATAGAG AAAAATCGAC CATGTGTAAG
  541 CGGCCAATCC GGTTCCACCC GAGATGCATA ATCTAGTAGA ATCTCTTCGC TATAAAAATT
  601 CACTTCCGCC TTCCACTCAC CGGTTGTCCA TTCATAGCTG AACTCTACTC CCTCTGTTGA
  661 CAGAACACAC ATCATCTCAA TATCCGAATA GGGCCCATCA GTCTGACGAC CAAGAGAGCC
  721 ATAAACACCA ATTGCCTTAA CATCATCCCC ATATTTATCC AATATTCGTT CCTTAATTTC
  781 ATGAACAATC TTCATTCTTT CTTCTCTAGT CATTATTATT GGTCCTTTCA TGAATTCTAG
  841 TCGACGCCGC ATGCAAGCGC CCGGTACGTC AGCGCGCGAT ACATGGCGCC GGTATCGATG
  901 TATACGTAGG AAAGCCGCTC AGCGATCCGT TTGGCGACCG TGCTTTTCCC CGCCGCGGCG
  961 GGTCCGTCGA TTGCGATGCT GATTCGTCGT TTCATCATTT CCTCCTAGCT TTACCGAAAA
 1021 TCGCTTGATG AGTGCACAAG AAAAATGCAG GAAAACCCCT GCATGTCTCC ATGTTTCATT
 1081 GTACCATATC GCCGGTCAAC GGGTACCGAG CTCGATCGTA TCAATGTCAT AGCCTGTTTC
1141 TGTGTGGTGA AATTTGTTAT CCGCTCCATA GTTGCTACAG TCAGAGCGCA CAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hoseki, J., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290.
user report Taniguchi, H., A Key Enzyme of the NAD+ Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures. J. Bacteriol. 199 (17). pii: e00359-17 (2017). PMID 28630126.
user report Ishida, K., Pseudouridine at position 55 in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus. Nucleic Acids Res., 39 (6): 2304-2318 (2011). PMID 21097467.
user report Shimada, A., A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways. Nucleic Acids Res., 38 (17), 5692-5705 (2010). PMID 20457749.

2018.12.10

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