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pUC18-HTK (with promoter)

Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene.

Catalog number RDB03436
Resource name pUC18-HTK (with promoter)
Clone info. Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene. It can be used to disrupt microbial genes using the kanamycin resistance genes.
Vector backbone pUC18 (Plasmid)
Gene/insert name Staphylococcus aureus kanamycin nucleotidyltransferase Genomic DNA
Reference of insert sequence AB121443 (DDBJ accession number)
Depositor|Developer Hoseki, Jun |
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External Database
Staphylococcus aureus kanamycin nucleotidyltransferase

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Biochem. (Tokyo)., 126, 951-956, 1999)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biochem. (Tokyo)., 126, 951-956, 1999)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB03436 pUC18-HTK (with promoter) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB03436.pdf

            Original reference

            original Hoseki, J., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13745_A7Kap1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: AFR
            Region: insert 5'( pro,HTK 5')
            Sequence file: RDB13745_A7Kac.seq check
            >03436_13745_A9EgF_AFR_D04_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNTGNN NNNGANNTCG AGCTCGGTAC CCGTTGACGG CGGANATGGT
               61 ACAATGAACA TGGAGACATG CAGGGTTTTC CTGCATTTTT CTTTGTGCAC TCATCAAGCG
              121 ATTTTCGGTA AAGCTAGGAG GAAATGATGA AACGACGAAT CAGCATCGCA ATCGACGGAC
              181 CCGCCGCGGC GGGGAAAAGC ACGGTCGCCA AACGGATCGC TGAGCGGCTT TCCTACGTAT
              241 ACATCGATAC CGGCGCCATG TATCGCGCGC TGACGTACCG GGCGCTTGCA TGCGGCGTCG
              301 ACTAGAATTC ATGAAAGGAC CAATAATAAT GACTAGAGAA GAAAGAATGA AGATTGTTCA
              361 TGAAATTAAG GAACGAATAT TGGATAAATA TGGGGATGAT GTTAAGGCAA TTGGTGTTTA
              421 TGGCTCTCTT GGTCGTCAGA CTGATGGGCC CTATTCGGAT ATTGAGATGA TGTGTGTTCT
              481 GTCAACAGAG GGAGTAGAGT TCAGCTATGA ATGGACAACC GGTGAGTGGA AGGCGGAAGT
              541 GAATTTTTAT AGCGAAGAGA TTCTACTAGA TTATGCATCT CGGGTGGAAC CGGATTGGCC
              601 GCTTACACAT GGTCGATTTT TCTCTATTTT GCCGATTTAT GATCCAGGTG GATACTTTGA
              661 GAAAGTGTAC CAAACTGCTA AATCGGTAGA AGCCCAAAAG TTCCACGATG CGATCTGTGC
              721 CCTTATCGTA NAAGAGCTGT TTGAATATGC AGGCAAATGG CGTAATATTC GTGTGCAAGG
              781 ACCGACAACA TTTCTACNAT CCTTGACTGT ACAGGTGGCA ATGNCANGTG CCATGTTGAT
              841 TGGTCTGCAT CATCGCATCT GTTATACNAC GAGCGCTTCN GTCTTAACTG AAGCANTTAA
              901 NCAACCANNT CTTCCTCCAG GTTATGTCCA ANTGTGCCAN CTCGNANTGT CTGGNCNNCT
              961 TTNCGANCNT GANAANNNTN NNNNNNNGCT AGANNNNTTC TGGNNNGGGG NNNCNNNNNN
            1021 NNNGNNNNNN NNNN
            //
            Primer: pUC-M13_-100
            Region: insert 3'(HTK 3')
            Sequence file: RDB13745_A7Kad.seq check
            >03436_13745_B1KoS_pUC-M13_-100_F02_17_ABI24.ab1
                1 NNNNNNNNNN CNNNNNNTTT CCCNGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT
               61 TGCATGTCTG CAGCGTAACC AACATGATTA ACAATTATTA GAGGTCATCG TTCAAAATGG
              121 TATGCGTTTT GACACATCCA CTATATATCC GTGTCGTTCC GCCCACTCCT GAACCCCATT
              181 CCAGAAATTC TCTAGCGATT CCAGAAGTTT CTCAGGGTCG GAAAGTTGAC CAGACATTAC
              241 GAGCTGGCAC AGTTGGACAT AACCTGGAGG AAGATCTGGT TGCTTAACTG CTTCAGTTAA
              301 GACCGAAGCG CTCGTCGTAT AACAGATGCG ATGATGCAGA CCAATCAACA TGGCACCTGC
              361 CATTGCCACC TGTACAGTCA AGGATGGTAG AAATGTTGTC GGTCCTTGCA CACGAATATT
              421 ACGCCATTTG CCTGCATATT CAAACAGCTC TTCTACGATA AGGGCACAGA TCGCATCGTG
              481 GAACTTTTGG GCTTCTACCG ATTTAGCAGT TTGGTACACT TTCTCAAAGT ATCCACCTGG
              541 ATCATAAATC GGCAAAATAG AGAAAAATCG ANCATGTGTA AGCGGCCAAT CCGGTTCCAC
              601 CCGANNNGCA TAATCTAGTA NAATCTCNTC GCTATNAAAN NTCACTTCNG CCTTNCACTC
              661 ANCGGNNGNC NNNTNNTANC TNNNNCNACT NNNCNGNNGN NNGNNNNCAN ATNNNCNNNN
            721 NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Hoseki, J., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290.
            user report Jolly, S.M., Thermus thermophilus Argonaute Functions in the Completion of DNA Replication. Cell 182 (6): 1545-1559.e18. (2020). PMID 32846159.
            user report Taniguchi, H., A Key Enzyme of the NAD+ Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures. J. Bacteriol. 199 (17). pii: e00359-17 (2017). PMID 28630126.
            user report Ishida, K., Pseudouridine at position 55 in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus. Nucleic Acids Res., 39 (6): 2304-2318 (2011). PMID 21097467.
            user report Shimada, A., A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways. Nucleic Acids Res., 38 (17), 5692-5705 (2010). PMID 20457749.

            2022.02.09

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