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pUC18-HTK (with promoter) (#RDB03436)

Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene.

Clone info. Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene. It can be used to disrupt microbial genes using the kanamycin resistance genes.
Vector backbone pUC18 (Plasmid)
Gene/insert name Staphylococcus aureus kanamycin nucleotidyltransferase Genomic DNA
Reference of insert sequence AB121443 (DDBJ accession number)
Depositor|Developer Hoseki, Jun |
 
Remarks, protocol and/or map (pdf) RDB03436.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Hoseki, J. et al., J. Biochem. (Tokyo)., 126, 951-956, 1999)
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hoseki, J. et al., J. Biochem. (Tokyo)., 126, 951-956, 1999)。

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RDB03436 pUC18-HTK (with promoter) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pUC18-HTK (with promoter) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03436).

Reference section:

Hoseki, J., Yano, T., Koyama, Y., Kuramitsu, S., Kagamiyama, H., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13745_A7Kap1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: AFR (Pr0324)
Region: insert 5'( pro,HTK 5')
Sequence file: RDB13745_A7Kac.seq check
>03436_13745_A9EgF_AFR_D04_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTGNN NNNGANNTCG AGCTCGGTAC CCGTTGACGG CGGANATGGT
   61 ACAATGAACA TGGAGACATG CAGGGTTTTC CTGCATTTTT CTTTGTGCAC TCATCAAGCG
  121 ATTTTCGGTA AAGCTAGGAG GAAATGATGA AACGACGAAT CAGCATCGCA ATCGACGGAC
  181 CCGCCGCGGC GGGGAAAAGC ACGGTCGCCA AACGGATCGC TGAGCGGCTT TCCTACGTAT
  241 ACATCGATAC CGGCGCCATG TATCGCGCGC TGACGTACCG GGCGCTTGCA TGCGGCGTCG
  301 ACTAGAATTC ATGAAAGGAC CAATAATAAT GACTAGAGAA GAAAGAATGA AGATTGTTCA
  361 TGAAATTAAG GAACGAATAT TGGATAAATA TGGGGATGAT GTTAAGGCAA TTGGTGTTTA
  421 TGGCTCTCTT GGTCGTCAGA CTGATGGGCC CTATTCGGAT ATTGAGATGA TGTGTGTTCT
  481 GTCAACAGAG GGAGTAGAGT TCAGCTATGA ATGGACAACC GGTGAGTGGA AGGCGGAAGT
  541 GAATTTTTAT AGCGAAGAGA TTCTACTAGA TTATGCATCT CGGGTGGAAC CGGATTGGCC
  601 GCTTACACAT GGTCGATTTT TCTCTATTTT GCCGATTTAT GATCCAGGTG GATACTTTGA
  661 GAAAGTGTAC CAAACTGCTA AATCGGTAGA AGCCCAAAAG TTCCACGATG CGATCTGTGC
  721 CCTTATCGTA NAAGAGCTGT TTGAATATGC AGGCAAATGG CGTAATATTC GTGTGCAAGG
  781 ACCGACAACA TTTCTACNAT CCTTGACTGT ACAGGTGGCA ATGNCANGTG CCATGTTGAT
  841 TGGTCTGCAT CATCGCATCT GTTATACNAC GAGCGCTTCN GTCTTAACTG AAGCANTTAA
  901 NCAACCANNT CTTCCTCCAG GTTATGTCCA ANTGTGCCAN CTCGNANTGT CTGGNCNNCT
  961 TTNCGANCNT GANAANNNTN NNNNNNNGCT AGANNNNTTC TGGNNNGGGG NNNCNNNNNN
1021 NNNGNNNNNN NNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: insert 3'(HTK 3')
Sequence file: RDB13745_A7Kad.seq check
>03436_13745_B1KoS_pUC-M13_-100_F02_17_ABI24.ab1
    1 NNNNNNNNNN CNNNNNNTTT CCCNGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT
   61 TGCATGTCTG CAGCGTAACC AACATGATTA ACAATTATTA GAGGTCATCG TTCAAAATGG
  121 TATGCGTTTT GACACATCCA CTATATATCC GTGTCGTTCC GCCCACTCCT GAACCCCATT
  181 CCAGAAATTC TCTAGCGATT CCAGAAGTTT CTCAGGGTCG GAAAGTTGAC CAGACATTAC
  241 GAGCTGGCAC AGTTGGACAT AACCTGGAGG AAGATCTGGT TGCTTAACTG CTTCAGTTAA
  301 GACCGAAGCG CTCGTCGTAT AACAGATGCG ATGATGCAGA CCAATCAACA TGGCACCTGC
  361 CATTGCCACC TGTACAGTCA AGGATGGTAG AAATGTTGTC GGTCCTTGCA CACGAATATT
  421 ACGCCATTTG CCTGCATATT CAAACAGCTC TTCTACGATA AGGGCACAGA TCGCATCGTG
  481 GAACTTTTGG GCTTCTACCG ATTTAGCAGT TTGGTACACT TTCTCAAAGT ATCCACCTGG
  541 ATCATAAATC GGCAAAATAG AGAAAAATCG ANCATGTGTA AGCGGCCAAT CCGGTTCCAC
  601 CCGANNNGCA TAATCTAGTA NAATCTCNTC GCTATNAAAN NTCACTTCNG CCTTNCACTC
  661 ANCGGNNGNC NNNTNNTANC TNNNNCNACT NNNCNGNNGN NNGNNNNCAN ATNNNCNNNN
721 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hoseki, J., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290. [link to RRC of NBRP]
user_report Jolly, S.M., Thermus thermophilus Argonaute Functions in the Completion of DNA Replication. Cell 182 (6): 1545-1559.e18. (2020). PMID 32846159. [link to RRC of NBRP]
user_report Taniguchi, H., A Key Enzyme of the NAD+ Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures. J. Bacteriol. 199 (17). pii: e00359-17 (2017). PMID 28630126. [link to RRC of NBRP]
user_report Ishida, K., Pseudouridine at position 55 in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus. Nucleic Acids Res., 39 (6): 2304-2318 (2011). PMID 21097467. [link to RRC of NBRP]
user_report Shimada, A., A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways. Nucleic Acids Res., 38 (17), 5692-5705 (2010). PMID 20457749. [link to RRC of NBRP]