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pUC18-HTK (with promoter) (#RDB03436)

Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hoseki, J., J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of highly thermostable kanamycin nucleotidyltransferase (HTK) gene. It can be used to disrupt microbial genes using the kanamycin resistance genes.
Vector backbone pUC18 (Plasmid)
Gene/insert name Staphylococcus aureus kanamycin nucleotidyltransferase Genomic DNA
Reference of insert sequence AB121443 (DDBJ accession number)
Depositor|Developer Hoseki, Jun |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Hoseki, J. et al., J. Biochem. (Tokyo)., 126, 951-956, 1999)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hoseki, J. et al., J. Biochem. (Tokyo)., 126, 951-956, 1999)。

Catalog # Resource name Shipping form Fee
RDB03436 pUC18-HTK (with promoter) DNA solution

How to cite this biological resource

Materials & Methods section:

The pUC18-HTK (with promoter) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03436).

Reference section:

Hoseki, J., Yano, T., Koyama, Y., Kuramitsu, S., Kagamiyama, H., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hoseki, J., Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus. J. Biochem. (Tokyo), 126, 951-956 (1999). PMID 10544290. [PubMed] [Article] [RRC of NBRP]
user_report Jolly, S.M., Thermus thermophilus Argonaute Functions in the Completion of DNA Replication. Cell 182 (6): 1545-1559.e18. (2020). PMID 32846159. [PubMed] [Article] [RRC of NBRP]
user_report Taniguchi, H., A Key Enzyme of the NAD+ Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures. J. Bacteriol. 199 (17). pii: e00359-17 (2017). PMID 28630126. [PubMed] [Article] [RRC of NBRP]
user_report Ishida, K., Pseudouridine at position 55 in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus. Nucleic Acids Res., 39 (6): 2304-2318 (2011). PMID 21097467. [PubMed] [Article] [RRC of NBRP]
user_report Shimada, A., A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways. Nucleic Acids Res., 38 (17), 5692-5705 (2010). PMID 20457749. [PubMed] [Article] [RRC of NBRP]