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Rab27A

Expression vector of mouse Rab27A cDNA.

Catalog number RDB03423
Resource name Rab27A
Clone info. cDNA encoding the FLAG-tagged Rab27A was subcloned into the NotI site of the modified pEF-BOS expression vector (J. Biol. Chem. (1994) 269, 29206-29211). Expression of FLAG-Rab27A promotes dense-core vesicle exocytosis in PC12 cells.
Expression was confirmed by depositor as follows: transfection into COS-7 cells and followed by both immunoblotting and immunocytochemistry. It is localized in secretory granule and melanosomme.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5 kb
Selectable markers Amp^r
Gene/insert name Mouse Rab27a cDNA
Reference of nucleotide sequence AB120511 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab27a (NCBI Gene 11891) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (J. Biol. Chem., 277, 39673-39678 (2002).; J. Biol. Chem., 277, 9212-9218 (2002)) and an acknowledgment to DEPOSITOR are requested.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03423 Rab27A DNA solution

Ordering Information [in Japanese] [in English]

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Electronic file

Electronic file Map RDB03423.gif from Depositor

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Featured content Mitsunori Fukuda's Rab panel clones (English text)
Featured content Organelle marker (English text)

References

original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.
original Fukuda, M., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990.

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.
original Fukuda, M., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990.

2018.12.10

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