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Rab27A

Expression vector of mouse Rab27A cDNA.

Catalog number RDB03423
Resource name Rab27A
Clone info. cDNA encoding the FLAG-tagged Rab27A was subcloned into the NotI site of the modified pEF-BOS expression vector (J. Biol. Chem. (1994) 269, 29206-29211). Expression of FLAG-Rab27A promotes dense-core vesicle exocytosis in PC12 cells.
Comment Expression was confirmed by depositor as follows: transfection into COS-7 cells and followed by both immunoblotting and immunocytochemistry. It is localized in secretory granule and melanosomme.
This clone contains a cDNA fragment corresponding to the 1nt to 666nt of AB232622.1 (1nt to 666nt in the case of AB120511.1). Known difference (at least) : T to G substitution at 246nt (Arg, synonym) of AB120511.1.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5 kb
Selectable markers Amp^r
Gene/insert name Mouse Rab27a cDNA
Reference of insert sequence AB120511, AB232622 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
Mouse Rab27a
          Reference sequence
            
           
          Sequence (full) RDB03423zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (J. Biol. Chem., 277, 39673-39678 (2002).; J. Biol. Chem., 277, 9212-9218 (2002)) and an acknowledgment to DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者は、研究成果の公表にあたって寄託者文献を引用する(J. Biol. Chem., 277, 39673-39678 (2002).; J. Biol. Chem., 277, 9212-9218 (2002))。利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とする。
          付加的提供条件：
          pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB03423 Rab27A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Rab27A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03423).

          Reference section:

          Kuroda, T.S., Fukuda, M., Ariga, H., Mikoshiba, K., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.
          Fukuda, M., Kanno, E., Saegusa, C., Ogata, Y., Kuroda, T.S., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Visualization of Organelles (English text)
          Featured content Rab family protein (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19159_B1Elp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: EF-1 alpha pro, FLAG, insert 5'
          Sequence file: RDB19159_B1Ela.seq check
          >03423_19159_B1El_1_EF1a-C_F_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTTTGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG
             61 TTCAAAGTTT TTTTCTTCCN TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA
            121 ATTCGATTCC ACCATGGACT ACAAGGACGA TGACGACAAG GGATCCATGT CGGATGGAGA
            181 TTACGATTAC CTCATCAAGT TCTTGGCCTT GGGAGACTCT GGGGTAGGGA AGACCAGTGT
            241 ACTCTACCAG TACACTGATG GCAAGTTCAA CTCCAAATTC ATCACCACAG TGGGCATTGA
            301 TTTCAGGGAA AAGAGAGTGG TGTACAGAGC CAATGGGCCA GATGGAGCTG TGGGCCGAGG
            361 CCAGAGAATC CACCTGCAGT TATGGGACAC GGCGGGGCAG GAGAGGTTTC GGAGCTTAAC
            421 CACTGCATTC TTCAGGGACG CTATGGGTTT CCTGCTTCTG TTCGACCTGA CAAATGAGCA
            481 AAGTTTCCTC AATGTCCGAA ACTGGATAAG CCAGCTACAG ATGNNNNCGT ACTGTGAAAA
            541 CCCAGATATA GTGCTGTGTG GAAATAAGAG TGACCTGGAA GANCNGAGGG CAGTGAAAGA
            601 GGAGGANGCC CGGGANCTTG CCGAGAAGTA CGGAATCCCC TATTTTGAAA CCNGTGCTGN
          661 CNACGGGNNA AACNTANNCN ACNNNNNTGA NANGNTCCNG NNNNNNNNNN
          //
          Primer: hGCSF_R (Pr0031)
          Region: hGCSF 3'UTR pA, insert 3'
          Sequence file: RDB19159_B1Elb.seq check
          >03423_19159_B1El_1_hGCSF_R_H03_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNCCNNGG NGGGGNNCCT CACTCTAGAG CGGCCGCGAA
             61 TTCACTAGTG ATTTCAACAG CCACACAACC CCTTCTCCTT CTCCTCACTT AGCTGATCCG
            121 CAGAGGTATG GCCGTTGGAC CGTACCACTC CCTCCGGAAT CCAGGACTTG TCCACACACC
            181 GCTCCATCCG CTTCATGATC AGGTCCAGGA GCATCTCAAT CGCGTGGCTT ATGTTTGTCC
            241 CGTTGGCAGC ACTGGTTTCA AAATAGGGGA TTCCGTACTT CTCGGCAAGT TCCCGGGCCT
            301 CCTCCTCTTT CACTGCCCTC TGGTCTTCCA GGTCACTCTT ATTTCCACAC AGCACTATAT
            361 CTGGGTTTTC ACAGTACGCG TGCATCTGTA GCTGGCTTAT CCAGTTTCGG ACATTGAGGA
            421 AACTTTGCTC ATTTGTCAGG TCGAACAGAA GCAGGAAACC CATAGCGTCC CTGAAGAATG
            481 CAGTGGTTAA GCTCCGAAAC CTCTCCTGCC CCGCCGTGTC CCATAACTGC AGGTGGATTC
            541 TCTGGCCTCG GCCCACAGCT CCNTCTGGCC CATTGGCTCT GTACACCACT CTCTTTTCCC
            601 TGNAANTCAA TGCCCACTGT GGTGATGAAT TTGGAGNTGA ACTTGCCNNN NNNGTACTGG
            661 TAGAGNANNN NNGNCNNNCC NACCCCNNAN TCNCCCNANG GNNNANAACN NGNNNNNNNA
          721 NNCNNAATCN NNNNNNANNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.
          original Fukuda, M., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl