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Rab27A (#RDB03423)

Expression vector of mouse Rab27A cDNA.

Clone info. cDNA encoding the FLAG-tagged Rab27A was subcloned into the NotI site of the modified pEF-BOS expression vector (J. Biol. Chem. (1994) 269, 29206-29211). Expression of FLAG-Rab27A promotes dense-core vesicle exocytosis in PC12 cells.
Comment Expression was confirmed by depositor as follows: transfection into COS-7 cells and followed by both immunoblotting and immunocytochemistry. It is localized in secretory granule and melanosomme.
This clone contains a cDNA fragment corresponding to the 1nt to 666nt of AB232622.1 (1nt to 666nt in the case of AB120511.1). Known difference (at least) : T to G substitution at 246nt (Arg, synonym) of AB120511.1.
Vector backbone pEF-BOS (RDB07939) (Plasmid)
Size of vector backbone 5 kb
Selectable markers Amp^r
Gene/insert name Mouse Rab27a cDNA
Reference of insert sequence AB120511, AB232622 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB03423zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (J. Biol. Chem., 277, 39673-39678 (2002).; J. Biol. Chem., 277, 9212-9218 (2002)) and an acknowledgment to DEPOSITOR are requested.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者は、研究成果の公表にあたって寄託者文献を引用する(J. Biol. Chem., 277, 39673-39678 (2002).; J. Biol. Chem., 277, 9212-9218 (2002))。利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03423 Rab27A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Rab27A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03423).

Reference section:

Kuroda, T.S., Fukuda, M., Ariga, H., Mikoshiba, K., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]
Fukuda, M., Kanno, E., Saegusa, C., Ogata, Y., Kuroda, T.S., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19159_B1Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro, FLAG, insert 5'
Sequence file: RDB19159_B1Ela.seq check
>03423_19159_B1El_1_EF1a-C_F_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTTTGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCN TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA
  121 ATTCGATTCC ACCATGGACT ACAAGGACGA TGACGACAAG GGATCCATGT CGGATGGAGA
  181 TTACGATTAC CTCATCAAGT TCTTGGCCTT GGGAGACTCT GGGGTAGGGA AGACCAGTGT
  241 ACTCTACCAG TACACTGATG GCAAGTTCAA CTCCAAATTC ATCACCACAG TGGGCATTGA
  301 TTTCAGGGAA AAGAGAGTGG TGTACAGAGC CAATGGGCCA GATGGAGCTG TGGGCCGAGG
  361 CCAGAGAATC CACCTGCAGT TATGGGACAC GGCGGGGCAG GAGAGGTTTC GGAGCTTAAC
  421 CACTGCATTC TTCAGGGACG CTATGGGTTT CCTGCTTCTG TTCGACCTGA CAAATGAGCA
  481 AAGTTTCCTC AATGTCCGAA ACTGGATAAG CCAGCTACAG ATGNNNNCGT ACTGTGAAAA
  541 CCCAGATATA GTGCTGTGTG GAAATAAGAG TGACCTGGAA GANCNGAGGG CAGTGAAAGA
  601 GGAGGANGCC CGGGANCTTG CCGAGAAGTA CGGAATCCCC TATTTTGAAA CCNGTGCTGN
661 CNACGGGNNA AACNTANNCN ACNNNNNTGA NANGNTCCNG NNNNNNNNNN
//
Primer: hGCSF_R (Pr0031)
Region: hGCSF 3'UTR pA, insert 3'
Sequence file: RDB19159_B1Elb.seq check
>03423_19159_B1El_1_hGCSF_R_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCCNNGG NGGGGNNCCT CACTCTAGAG CGGCCGCGAA
   61 TTCACTAGTG ATTTCAACAG CCACACAACC CCTTCTCCTT CTCCTCACTT AGCTGATCCG
  121 CAGAGGTATG GCCGTTGGAC CGTACCACTC CCTCCGGAAT CCAGGACTTG TCCACACACC
  181 GCTCCATCCG CTTCATGATC AGGTCCAGGA GCATCTCAAT CGCGTGGCTT ATGTTTGTCC
  241 CGTTGGCAGC ACTGGTTTCA AAATAGGGGA TTCCGTACTT CTCGGCAAGT TCCCGGGCCT
  301 CCTCCTCTTT CACTGCCCTC TGGTCTTCCA GGTCACTCTT ATTTCCACAC AGCACTATAT
  361 CTGGGTTTTC ACAGTACGCG TGCATCTGTA GCTGGCTTAT CCAGTTTCGG ACATTGAGGA
  421 AACTTTGCTC ATTTGTCAGG TCGAACAGAA GCAGGAAACC CATAGCGTCC CTGAAGAATG
  481 CAGTGGTTAA GCTCCGAAAC CTCTCCTGCC CCGCCGTGTC CCATAACTGC AGGTGGATTC
  541 TCTGGCCTCG GCCCACAGCT CCNTCTGGCC CATTGGCTCT GTACACCACT CTCTTTTCCC
  601 TGNAANTCAA TGCCCACTGT GGTGATGAAT TTGGAGNTGA ACTTGCCNNN NNNGTACTGG
  661 TAGAGNANNN NNGNCNNNCC NACCCCNNAN TCNCCCNANG GNNNANAACN NGNNNNNNNA
721 NNCNNAATCN NNNNNNANNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]
original Fukuda, M., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990. [link to RRC of NBRP]