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Slac2-a

Expression vector of mouse Slp homologue lacking C2 lomains-a (Slac2a) cDNA

Catalog number RDB03422
Resource name Slac2-a
Alternative name melanophilin
Clone info. cDNA encoding the T7-tagged Slac2-a was subcloned into the NotI site of the modified pEF-BOS expression vector (J. Biol. Chem. (1994) 269, 29206-29211). Recombinant Slac2-a is localized on melanosomes through interaction with Rab27A and regulates melanosome transport in melanocytes. Expression was confirmed by depositor as follows: transfection into COS-7 cells followed by both immunoblotting and immunocytochemistry.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5 kbp
Selectable markers Amp^r
Gene/insert name Mouse Slac2-a/Mlph cDNA
Reference of nucleotide sequence AB057759 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Mlph (NCBI Gene 171531) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Biochem. Biophys. Res. Commun., 283, 513-519 (2001).; J. Biol. Chem., 277, 9212-9218 (2002).; J. Biol. Chem., 277, 12432-12436 (2002)) and an acknowledgment to DEPOSITOR are requested.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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RDB03422 Slac2-a DNA solution

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Electronic file

Electronic file Map RDB03422.gif from Depositor

Featured content

Featured content Mitsunori Fukuda's Rab panel clones (English text)

References

original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.
original Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.
original Fukuda, M., Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain. Biochem. Biophys. Res. Commun., 283, 513-519 (2001). PMID 11327731.

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.
original Fukuda, M., Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport. J. Biol. Chem., 277, 12432-12436 (2002). PMID 11856727.
original Fukuda, M., Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain. Biochem. Biophys. Res. Commun., 283, 513-519 (2001). PMID 11327731.

2018.12.10

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