Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Slp4-a (#RDB03421)

Expression vector of mouse synaptotagmin-like protein 4-a(Slp4-a) cDNA

Alternative name granuphilin-a
Clone info. cDNA encoding the T7-tagged Slp4-a was subcloned into the NotI site of the modified pEF-BOS expression vector (J. Biol. Chem. (1994) 269, 29206-29211). Expression of T7-Slp4-a inhibits dense-core vesicle exocytosis through specific interaction with Rab27A. Expression was confirmed by depositor as follows: transfection into COS-7 cells followed by both immunoblotting and immunocytochemistry.
Comment This clone contains a cDNA fragment corresponding to the 186nt to 2207nt of AB025258.1 (1nt to 2022nt in the case of AB120510.1). Known differences (at least) : A to C substitution at 1953nt (Arg, synonym) of AB025258.1 (at 1768nt in the case of AB120510.1).
Vector backbone pEF-BOS (RDB07939) (Plasmid)
Size of vector backbone 5 kb
Selectable markers Amp^r
Gene/insert name Mouse Slp4-a (synaptotagmin-like protein 4-a) cDNA
Reference of insert sequence AB120510 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (full) RDB03421zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (J. Biol. Chem., 277, 39673-39678 (2002).; J. Biol. Chem., 278, 15390-15396 (2003).) and an acknowledgment to DEPOSITOR are requested.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Biol. Chem., 277, 39673-39678 (2002).; J. Biol. Chem., 278, 15390-15396 (2003))を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
備考


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03421 Slp4-a DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Slp4-a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03421).

Reference section:

Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134. [link to RRC of NBRP]
Fukuda, M., Kanno, E., Saegusa, C., Ogata, Y., Kuroda, T.S., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19158_B1Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro, T7 tag, insert 5'
Sequence file: RDB19158_B1Ela.seq check
>03421_19158_B1El_1_EF1a-C_F_D03_12_ABI24.ab1
    1 NNNNNNNNNN CNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT
  121 TCGATTCCAC CATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCCATGTCGG
  181 AGATACTAGA CCTCTCTTTT CTGTCCGAGA TGGAAAGGGA TTTGATCCTC GGTGTTCTGC
  241 AGAGAGATGA AGAACTCAGA AAAGCAGATG AGAAAAGGAT TCGGCGACTG AAGAATGAGC
  301 TCCTGGAGAT CAAAAGAAAA GGGGCCAAAA GGGGCAGCCA ACACTATAGT GATCGAACCT
  361 GTGCCAGGTG CCAGGAGGGC CTGGGCCGCT TAATTCCCAA AAGCAGCACT TGTGTGGGCT
  421 GCAATCATTT AGTGTGCCGA GAATGCCGAG TTCTGGAAAG CAATGGCTCT TGGAGGTGCA
  481 AGGTGTGCTC GAAGGAGATA GAGCTGAAGA AAGCAACTGG AGATTGGTTT TATGACCAGA
  541 AAGTAAATCG CTTTGATTAC CGAACAGGCA GCGAGANAAT CAGGATGTCC CTGCGCCAGA
  601 NNCCTGCAGT GAANNAAAAG AGAGACAGCG GGGGCAGTCC CTNCTTCNGC NGANACAGAT
  661 GGGTGNNATC TGGNCNGNAA NAANNNTCNN TNNAGANNNN CNACNGANGG AANNAANTGT
721 GNNNNTNGNN NNNNNNANAN CNNNNNNNNN N
//
Primer: hGCSF_R (Pr0031)
Region: insert 3'
Sequence file: RDB19158_B1Elb.seq check
>03421_19158_B1El_1_hGCSF_R_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTCCNNGGTG GGGANCCTCA CNNCTAGAGC GGCCGCTATG
   61 GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTGCGA ATTCATACAC CCAGCTTCTG
  121 CTTGACCATT GAAGAACGGA GCTGCAGAGT TCCTTCTGCC CAAGACCCTG GGTACTGTCG
  181 CATCTTCTGC CACAAGCTTA CTTCTTCACC AGTCGAATCC ATCCAGTCCA CCACTTCGCC
  241 ATTACTGATC CCAGTGCCAA CACCCAGTCT GACCCCTCCC AGGAAGTCAT TGCTGGCCAG
  301 GGGCTCCCGG TCCCATACAG TCAGTTCCAG GCACATGTGT TGTAGATCTT CCAGCCGCAC
  361 ACCATTGTAG ACAAATGTAT GGTTGTAGTG GGGACTCAGA GTCTTCTTCA TCACAGGAGT
  421 TTTACGCTTA CTGGCCTTGT TCCTCATGGG AAGGAGGTAT CCCTTGACAA AGCTGTCTGA
  481 AGTCCCTCCT GATTTGGCAG CCGTCAGGTT CTTGGCTTCT TTGATCCACA CCTGGAGTTC
  541 TCCCCCTTCC CCACCTTTAC TCTTTTTCCG GTCACCTCCA ACAGGGAGTT TGGANGCTGG
  601 GATGTANTTC AATGAANNNN NNNACTCGCC TTTGTGTGCT GGCAAACCNG GCGAGGANTC
  661 NNNACTGNTC NTTCCNNGNA AGGNNNGCAN NGATNCANNN NCNNNNNCNN CTTNCNNNAN
721 TNNNNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134. [link to RRC of NBRP]
original Fukuda, M., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990. [link to RRC of NBRP]