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Slp4-a

Expression vector of mouse synaptotagmin-like protein 4-a(Slp4-a) cDNA

Catalog number RDB03421
Resource name Slp4-a
Alternative name granuphilin-a
Clone info. cDNA encoding the T7-tagged Slp4-a was subcloned into the NotI site of the modified pEF-BOS expression vector (J. Biol. Chem. (1994) 269, 29206-29211). Expression of T7-Slp4-a inhibits dense-core vesicle exocytosis through specific interaction with Rab27A. Expression was confirmed by depositor as follows: transfection into COS-7 cells followed by both immunoblotting and immunocytochemistry.
This clone contains a cDNA fragment corresponding to the 186nt to 2207nt of AB025258.1 (1nt to 2022nt in the case of AB120510.1). Known differences (at least) : A to C substitution at 1953nt (Arg, synonym) of AB025258.1 (at 1768nt in the case of AB120510.1).
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5 kb
Selectable markers Amp^r
Gene/insert name Mouse Slp4-a (synaptotagmin-like protein 4-a) cDNA
Reference of nucleotide sequence AB120510, AB025258 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Sytl4 (NCBI Gene 27359) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (J. Biol. Chem., 277, 39673-39678 (2002).; J. Biol. Chem., 278, 15390-15396 (2003).) and an acknowledgment to DEPOSITOR are requested.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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RDB03421 Slp4-a DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB03421zzt01.seq compiled by us

Original reference

original Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134.
original Fukuda, M., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19158_B1Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF-1 alpha pro, T7 tag, insert 5'
Sequence file: RDB19158_B1Ela.seq check
>03421_19158_B1El_1_EF1a-C_F_D03_12_ABI24.ab1
    1 NNNNNNNNNN CNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT
  121 TCGATTCCAC CATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCCATGTCGG
  181 AGATACTAGA CCTCTCTTTT CTGTCCGAGA TGGAAAGGGA TTTGATCCTC GGTGTTCTGC
  241 AGAGAGATGA AGAACTCAGA AAAGCAGATG AGAAAAGGAT TCGGCGACTG AAGAATGAGC
  301 TCCTGGAGAT CAAAAGAAAA GGGGCCAAAA GGGGCAGCCA ACACTATAGT GATCGAACCT
  361 GTGCCAGGTG CCAGGAGGGC CTGGGCCGCT TAATTCCCAA AAGCAGCACT TGTGTGGGCT
  421 GCAATCATTT AGTGTGCCGA GAATGCCGAG TTCTGGAAAG CAATGGCTCT TGGAGGTGCA
  481 AGGTGTGCTC GAAGGAGATA GAGCTGAAGA AAGCAACTGG AGATTGGTTT TATGACCAGA
  541 AAGTAAATCG CTTTGATTAC CGAACAGGCA GCGAGANAAT CAGGATGTCC CTGCGCCAGA
  601 NNCCTGCAGT GAANNAAAAG AGAGACAGCG GGGGCAGTCC CTNCTTCNGC NGANACAGAT
  661 GGGTGNNATC TGGNCNGNAA NAANNNTCNN TNNAGANNNN CNACNGANGG AANNAANTGT
721 GNNNNTNGNN NNNNNNANAN CNNNNNNNNN N
//
Primer: hGCSF_R
Region: insert 3'
Sequence file: RDB19158_B1Elb.seq check
>03421_19158_B1El_1_hGCSF_R_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTCCNNGGTG GGGANCCTCA CNNCTAGAGC GGCCGCTATG
   61 GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTGCGA ATTCATACAC CCAGCTTCTG
  121 CTTGACCATT GAAGAACGGA GCTGCAGAGT TCCTTCTGCC CAAGACCCTG GGTACTGTCG
  181 CATCTTCTGC CACAAGCTTA CTTCTTCACC AGTCGAATCC ATCCAGTCCA CCACTTCGCC
  241 ATTACTGATC CCAGTGCCAA CACCCAGTCT GACCCCTCCC AGGAAGTCAT TGCTGGCCAG
  301 GGGCTCCCGG TCCCATACAG TCAGTTCCAG GCACATGTGT TGTAGATCTT CCAGCCGCAC
  361 ACCATTGTAG ACAAATGTAT GGTTGTAGTG GGGACTCAGA GTCTTCTTCA TCACAGGAGT
  421 TTTACGCTTA CTGGCCTTGT TCCTCATGGG AAGGAGGTAT CCCTTGACAA AGCTGTCTGA
  481 AGTCCCTCCT GATTTGGCAG CCGTCAGGTT CTTGGCTTCT TTGATCCACA CCTGGAGTTC
  541 TCCCCCTTCC CCACCTTTAC TCTTTTTCCG GTCACCTCCA ACAGGGAGTT TGGANGCTGG
  601 GATGTANTTC AATGAANNNN NNNACTCGCC TTTGTGTGCT GGCAAACCNG GCGAGGANTC
  661 NNNACTGNTC NTTCCNNGNA AGGNNNGCAN NGATNCANNN NCNNNNNCNN CTTNCNNNAN
721 TNNNNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134.
original Fukuda, M., Slp4-a/granuphilin-a regulates dense-core vesicle exocytosis in PC12 cells. J. Biol. Chem., 277, 39673-39678 (2002). PMID 12176990.

2021.07.13

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