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Human HLA-C*15:02:01 cDNA

Human HLA-C*15:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03379
Resource name Human HLA-C*15:02:01 cDNA
Alternative name HLA-Cw*15021
Clone info. Human HLA-C*15:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*15021 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03379 Human HLA-C*15:02:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03379z.seq from Depositor
Electronic file Map RDB03379.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13530_A5Iup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13530_A5Iua.seq
>03379_13530_A5Iu_T7_20bp_A10_01.ab1
    1 CGTGCTGCCG TTAACTTAGC TTTTCTCCCC AGACGCCGAG ATGCGGGTCA TGGCGCCCCG
   61 AACCCTCCTC CTGCTGCTCT CGGGAGCCCT GGCCCTGACC GAGACCTGGG CCTGCTCCCA
  121 CTCCATGAGG TATTTCTACA CCGCTGTGTC CCGGCCCGGC CGCGGAGAGC CCCACTTCAT
  181 CGCAGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC GACAGCGACG CCGCGAGTCC
  241 AAGAGGGGAG CCGCGGGCGC CGTGGGTGGA GCAGGAGGGG CCGGAGTATT GGGACCGGGA
  301 GACACAGAAC TACAAGCGCC AGGCACAGAC TGACCGAGTG AACCTGCGGA AACTGCGCGG
  361 CTACTACAAC CAGAGCGAGG CCGGGTCTCA CATCATCCAG AGGATGTATG GCTGCGACCT
  421 GGGGCCCGAC GGGCGCCTCC TCCGCGGGCA TGACCAGTTA GCCTACGACG GCAAGGATTA
  481 CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCCGCG GACACGGCGG CTCAGATCAC
  541 CCAGCGCAAG TGGGAGGCGG CCCGTGAGGC GGAGCAGCTG AGAGCCTACC TGGAGGGCAC
  601 GTGCGTGGAG TGGCTCCGCA GATACCTGGA AGAACGGGAA GGAGACGCTG CACCGCGCGG
661 AAACACCAAA AATCTACG
//
Primer: BGH_rev RDB13530_A5Iub.seq
>03379_13530_A5Iu_T7_BGHrev_B04_04.ab1
    1 CGGGTTCGGG GGCGTCTAGA CTCGAGCGGC CGCGTCTCAG GCTTTACAAG CGATGAGAGA
   61 CTCATCAGAG CCCTGGGCAC TGTTGCTGGA CGCAGCCTGA GAGCAGCTCC CTCCTTTTCC
  121 ACCTGAGCTC TTCCTCCTAC ACATCACAAC AGCCATCACA GCTCCTAGGA CAGCTAGGAC
  181 AGCCAGGACA GCCAGGCCAG CAACGATGCC CACGATGGGG ATGGTGGGCT GGGAAGATGG
  241 CTCCCATCTC AGGGTGAGGG GCTCCGGCAG CCCCTCGTGC TGCACATGGC ACGTGTATCT
  301 CTGCTCTTCT CCAGAAGGCA CCACCACAGC TGCCCACTTC TGGAAGGTTC CATCTCCTGC
  361 TGGCCTGGTC TCCACAAGCT CGGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT
  421 CAGTGTGATC TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC
  481 AGAGACGGGA TGGTGGGTCA CGTGTGTCTT TGGGTGTTCC GCGCGCTGCA GCGTCTCCTT
  541 CCCGTTCTCC AGGTATCTGC GGAGCCACTC CACGCACGTG CCCTCCAGGT AGGCTCTCAG
  601 CTGCTCCGCC TCACGGGCCG CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CCGTGTCCGC
  661 GGCGGTCCAG GAGCGCAGGT CCTCGTTCAG GGCGATGTAA TCCTTGCCGT CGTAGGCTAA
  721 CTGGTCATGC CCGCGGAGGA GGCGCCCGTC GGGCCCCAGG TCGCAGCCAT ACATCCTCTG
  781 GATGATGTGA GACCCGGCCT CGCTCTGGTT GTAGTAGCCG CGCAGTTTCC GCAGGTTCAC
  841 TCGGTCAGTC TGTGCCTGGC GCTTGTAGTT CTGTGTCTCC CGGTCCCAAT ACTCCGGCCC
  901 CTCCTGCTCC ACCCACGGCG CCCGCGGCTC CCCTCTTGGA CTCGCGGCGT CGCTGTCGAA
  961 CCGCACGAAC TGCGTGTCGT CCACGTAGCC CACTGCGATG AAGTGGGGGC TCTCCGCGGC
 1021 CGGGCCGGGA CACAGCGGGT GTAGAAAATA CCCTCATGGG AGTGGGAGCA GGGCCCAGGT
 1081 CTCGGGTCAA GGGACAGGCT CCCGAGAGCA GCAGGGAGAA GGTCGGGCGT CAATGAACCC
1141 GCATCTCGCC GTCTTGGGGA GAAAAAAGCC TTAAAGGTTT TTAC
//
Primer: SV40pro_F RDB13530_A5Iuc.seq
>03379_13530_A5Iu_T7_SV40pro_F_C04_07.ab1
    1 ACCTCGTTCC CTGGACATTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
  121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
  661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
  961 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG GCCTTCTATG AAAGGTTGGG
 1021 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GGATGATCCT CCAGCGCGGG GATCTCATGC
 1081 TGGAGGTTCT TCGCCCACCC CACTGTTTTA TGCAGCTATA TGCTACAATT AGCCAATAGC
1141 CATCACAAAT TTCCAACAAA TAAAGGCCAC TTTTTTTTTC T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.
user report Ichise, H., NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs. Stem Cell Reports 9 (3): 853-867 (2017). PMID 28867344.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl