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Human HLA-C*15:02:01 cDNA

Human HLA-C*15:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03379
Resource name Human HLA-C*15:02:01 cDNA
Alternative name HLA-Cw*15021
Clone info. Human HLA-C*15:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*15021 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*15021

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB03379 Human HLA-C*15:02:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB03379z.seq
          Remarks, protocol and/or map (gif) RDB03379.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13530_A5Iup1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp
          Sequence file: RDB13530_A5Iua.seq check
          >03379_13530_A5Iu_T7_20bp_A10_01.ab1
              1 CGTGCTGCCG TTAACTTAGC TTTTCTCCCC AGACGCCGAG ATGCGGGTCA TGGCGCCCCG
             61 AACCCTCCTC CTGCTGCTCT CGGGAGCCCT GGCCCTGACC GAGACCTGGG CCTGCTCCCA
            121 CTCCATGAGG TATTTCTACA CCGCTGTGTC CCGGCCCGGC CGCGGAGAGC CCCACTTCAT
            181 CGCAGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC GACAGCGACG CCGCGAGTCC
            241 AAGAGGGGAG CCGCGGGCGC CGTGGGTGGA GCAGGAGGGG CCGGAGTATT GGGACCGGGA
            301 GACACAGAAC TACAAGCGCC AGGCACAGAC TGACCGAGTG AACCTGCGGA AACTGCGCGG
            361 CTACTACAAC CAGAGCGAGG CCGGGTCTCA CATCATCCAG AGGATGTATG GCTGCGACCT
            421 GGGGCCCGAC GGGCGCCTCC TCCGCGGGCA TGACCAGTTA GCCTACGACG GCAAGGATTA
            481 CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCCGCG GACACGGCGG CTCAGATCAC
            541 CCAGCGCAAG TGGGAGGCGG CCCGTGAGGC GGAGCAGCTG AGAGCCTACC TGGAGGGCAC
            601 GTGCGTGGAG TGGCTCCGCA GATACCTGGA AGAACGGGAA GGAGACGCTG CACCGCGCGG
          661 AAACACCAAA AATCTACG
          //
          Primer: BGH_rev
          Sequence file: RDB13530_A5Iub.seq check
          >03379_13530_A5Iu_T7_BGHrev_B04_04.ab1
              1 CGGGTTCGGG GGCGTCTAGA CTCGAGCGGC CGCGTCTCAG GCTTTACAAG CGATGAGAGA
             61 CTCATCAGAG CCCTGGGCAC TGTTGCTGGA CGCAGCCTGA GAGCAGCTCC CTCCTTTTCC
            121 ACCTGAGCTC TTCCTCCTAC ACATCACAAC AGCCATCACA GCTCCTAGGA CAGCTAGGAC
            181 AGCCAGGACA GCCAGGCCAG CAACGATGCC CACGATGGGG ATGGTGGGCT GGGAAGATGG
            241 CTCCCATCTC AGGGTGAGGG GCTCCGGCAG CCCCTCGTGC TGCACATGGC ACGTGTATCT
            301 CTGCTCTTCT CCAGAAGGCA CCACCACAGC TGCCCACTTC TGGAAGGTTC CATCTCCTGC
            361 TGGCCTGGTC TCCACAAGCT CGGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT
            421 CAGTGTGATC TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC
            481 AGAGACGGGA TGGTGGGTCA CGTGTGTCTT TGGGTGTTCC GCGCGCTGCA GCGTCTCCTT
            541 CCCGTTCTCC AGGTATCTGC GGAGCCACTC CACGCACGTG CCCTCCAGGT AGGCTCTCAG
            601 CTGCTCCGCC TCACGGGCCG CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CCGTGTCCGC
            661 GGCGGTCCAG GAGCGCAGGT CCTCGTTCAG GGCGATGTAA TCCTTGCCGT CGTAGGCTAA
            721 CTGGTCATGC CCGCGGAGGA GGCGCCCGTC GGGCCCCAGG TCGCAGCCAT ACATCCTCTG
            781 GATGATGTGA GACCCGGCCT CGCTCTGGTT GTAGTAGCCG CGCAGTTTCC GCAGGTTCAC
            841 TCGGTCAGTC TGTGCCTGGC GCTTGTAGTT CTGTGTCTCC CGGTCCCAAT ACTCCGGCCC
            901 CTCCTGCTCC ACCCACGGCG CCCGCGGCTC CCCTCTTGGA CTCGCGGCGT CGCTGTCGAA
            961 CCGCACGAAC TGCGTGTCGT CCACGTAGCC CACTGCGATG AAGTGGGGGC TCTCCGCGGC
           1021 CGGGCCGGGA CACAGCGGGT GTAGAAAATA CCCTCATGGG AGTGGGAGCA GGGCCCAGGT
           1081 CTCGGGTCAA GGGACAGGCT CCCGAGAGCA GCAGGGAGAA GGTCGGGCGT CAATGAACCC
          1141 GCATCTCGCC GTCTTGGGGA GAAAAAAGCC TTAAAGGTTT TTAC
          //
          Primer: SV40pro_F
          Sequence file: RDB13530_A5Iuc.seq check
          >03379_13530_A5Iu_T7_SV40pro_F_C04_07.ab1
              1 ACCTCGTTCC CTGGACATTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
             61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
            121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
            661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
            781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
            841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
            901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG
            961 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG GCCTTCTATG AAAGGTTGGG
           1021 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GGATGATCCT CCAGCGCGGG GATCTCATGC
           1081 TGGAGGTTCT TCGCCCACCC CACTGTTTTA TGCAGCTATA TGCTACAATT AGCCAATAGC
          1141 CATCACAAAT TTCCAACAAA TAAAGGCCAC TTTTTTTTTC T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.
          user report Ichise, H., NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs. Stem Cell Reports 9 (3): 853-867 (2017). PMID 28867344.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl