Human HLA-C*15:02:01 cDNA from human EBV-transformed B cell line cDNA
| Catalog number | RDB03379 |
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| Resource name | Human HLA-C*15:02:01 cDNA |
| Alternative name | HLA-Cw*15021 |
| Clone info. | Human HLA-C*15:02:01 cDNA from human EBV-transformed B cell line cDNA. |
| Vector backbone | pcDNA3.1(+) (Plasmid) |
| Size of vector backbone | 5.4 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human HLA-Cw*15021 cDNA |
| Depositor | Akatsuka, Yoshiki | |
| Other clones in our bank | human HLA-C (NCBI Gene 3107) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).) |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB03379 | Human HLA-C*15:02:01 cDNA | DNA solution |
Electronic file
| Electronic file | Sequence RDB03379z.seq from Depositor |
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| Electronic file | Map RDB03379.gif from Depositor
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Featured content
| Featured content | HLA Clones (en) (English text) |
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| Featured content | HLA Clones (ja) (Japanese text) |
References
| original | Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13530_A5Iup1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB13530_A5Iua.seq |
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>03379_13530_A5Iu_T7_20bp_A10_01.ab1 1 CGTGCTGCCG TTAACTTAGC TTTTCTCCCC AGACGCCGAG ATGCGGGTCA TGGCGCCCCG 61 AACCCTCCTC CTGCTGCTCT CGGGAGCCCT GGCCCTGACC GAGACCTGGG CCTGCTCCCA 121 CTCCATGAGG TATTTCTACA CCGCTGTGTC CCGGCCCGGC CGCGGAGAGC CCCACTTCAT 181 CGCAGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC GACAGCGACG CCGCGAGTCC 241 AAGAGGGGAG CCGCGGGCGC CGTGGGTGGA GCAGGAGGGG CCGGAGTATT GGGACCGGGA 301 GACACAGAAC TACAAGCGCC AGGCACAGAC TGACCGAGTG AACCTGCGGA AACTGCGCGG 361 CTACTACAAC CAGAGCGAGG CCGGGTCTCA CATCATCCAG AGGATGTATG GCTGCGACCT 421 GGGGCCCGAC GGGCGCCTCC TCCGCGGGCA TGACCAGTTA GCCTACGACG GCAAGGATTA 481 CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCCGCG GACACGGCGG CTCAGATCAC 541 CCAGCGCAAG TGGGAGGCGG CCCGTGAGGC GGAGCAGCTG AGAGCCTACC TGGAGGGCAC 601 GTGCGTGGAG TGGCTCCGCA GATACCTGGA AGAACGGGAA GGAGACGCTG CACCGCGCGG 661 AAACACCAAA AATCTACG // |
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| Primer: BGH_rev | RDB13530_A5Iub.seq |
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>03379_13530_A5Iu_T7_BGHrev_B04_04.ab1 1 CGGGTTCGGG GGCGTCTAGA CTCGAGCGGC CGCGTCTCAG GCTTTACAAG CGATGAGAGA 61 CTCATCAGAG CCCTGGGCAC TGTTGCTGGA CGCAGCCTGA GAGCAGCTCC CTCCTTTTCC 121 ACCTGAGCTC TTCCTCCTAC ACATCACAAC AGCCATCACA GCTCCTAGGA CAGCTAGGAC 181 AGCCAGGACA GCCAGGCCAG CAACGATGCC CACGATGGGG ATGGTGGGCT GGGAAGATGG 241 CTCCCATCTC AGGGTGAGGG GCTCCGGCAG CCCCTCGTGC TGCACATGGC ACGTGTATCT 301 CTGCTCTTCT CCAGAAGGCA CCACCACAGC TGCCCACTTC TGGAAGGTTC CATCTCCTGC 361 TGGCCTGGTC TCCACAAGCT CGGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT 421 CAGTGTGATC TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC 481 AGAGACGGGA TGGTGGGTCA CGTGTGTCTT TGGGTGTTCC GCGCGCTGCA GCGTCTCCTT 541 CCCGTTCTCC AGGTATCTGC GGAGCCACTC CACGCACGTG CCCTCCAGGT AGGCTCTCAG 601 CTGCTCCGCC TCACGGGCCG CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CCGTGTCCGC 661 GGCGGTCCAG GAGCGCAGGT CCTCGTTCAG GGCGATGTAA TCCTTGCCGT CGTAGGCTAA 721 CTGGTCATGC CCGCGGAGGA GGCGCCCGTC GGGCCCCAGG TCGCAGCCAT ACATCCTCTG 781 GATGATGTGA GACCCGGCCT CGCTCTGGTT GTAGTAGCCG CGCAGTTTCC GCAGGTTCAC 841 TCGGTCAGTC TGTGCCTGGC GCTTGTAGTT CTGTGTCTCC CGGTCCCAAT ACTCCGGCCC 901 CTCCTGCTCC ACCCACGGCG CCCGCGGCTC CCCTCTTGGA CTCGCGGCGT CGCTGTCGAA 961 CCGCACGAAC TGCGTGTCGT CCACGTAGCC CACTGCGATG AAGTGGGGGC TCTCCGCGGC 1021 CGGGCCGGGA CACAGCGGGT GTAGAAAATA CCCTCATGGG AGTGGGAGCA GGGCCCAGGT 1081 CTCGGGTCAA GGGACAGGCT CCCGAGAGCA GCAGGGAGAA GGTCGGGCGT CAATGAACCC 1141 GCATCTCGCC GTCTTGGGGA GAAAAAAGCC TTAAAGGTTT TTAC // |
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| Primer: SV40pro_F | RDB13530_A5Iuc.seq |
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>03379_13530_A5Iu_T7_SV40pro_F_C04_07.ab1 1 ACCTCGTTCC CTGGACATTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 961 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG GCCTTCTATG AAAGGTTGGG 1021 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GGATGATCCT CCAGCGCGGG GATCTCATGC 1081 TGGAGGTTCT TCGCCCACCC CACTGTTTTA TGCAGCTATA TGCTACAATT AGCCAATAGC 1141 CATCACAAAT TTCCAACAAA TAAAGGCCAC TTTTTTTTTC T // |
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Sequencing and PCR primers for primer information.
References
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl