Resource data sheet
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Human HLA-C*06:02 cDNA

Human HLA-C*06:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03377
Resource name Human HLA-C*06:02 cDNA
Alternative name HLA-Cw*0602
Clone info. Human HLA-C*06:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0602 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03377 Human HLA-C*06:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03377z.seq from Depositor
Electronic file Map RDB03377.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13529_A5Iup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13529_A5Iua.seq
>03377_13529_A5Iu_T7_20bp_A02_02.ab1
    1 CATACACGTT TAACTTAGCT TTTCTCCCCA GACGCCGAGA TGCGGGTCAT GGCGCCCCGA
   61 ACCCTCATCC TGCTGCTCTC GGGAGCCCTG GCCCTGACCG AGACCTGGGC CTGCTCCCAC
  121 TCCATGAGGT ATTTCGACAC CGCCGTGTCC CGGCCCGGCC GCGGAGAGCC CCGCTTCATC
  181 TCAGTGGGCT ACGTGGACGA CACGCAGTTC GTGCGGTTCG ACAGCGACGC CGCGAGTCCG
  241 AGAGGGGAGC CCCGGGCGCC GTGGGTGGAG CAGGAGGGGC CGGAGTATTG GGACCGGGAG
  301 ACACAGAAGT ACAAGCGCCA GGCACAGGCT GACCGAGTGA ACCTGCGGAA ACTGCGCGGC
  361 TACTACAACC AGAGCGAGGA CGGGTCTCAC ACCCTCCAGT GGATGTATGG CTGCGACCTG
  421 GGGCCCGACG GGCGCCTCCT CCGCGGGTAT GACCAGTCCG CCTACGACGG CAAGGATTAC
  481 ATCGCCCTGA ACGAGGACCT GCGCTCCTGG ACCGCCGCGG ACACGGCGGC TCAGATCACC
  541 CAGCGCAAGT GGGAGGCGGC CCGTGAGGCG GAGCAGTGGA GAGCCTACCT GGAGGGCACG
  601 TGCGTGGAGT GGCTCCGCAG ATACCTGGAG AACGGGAAGG AGACGCTGCA GCGCGCGGAA
  661 CACCCAAAGA CACACGTGAC CCACCATCCC GTCTCTGACC ATGAGGCCAC CCTGAGGTGC
  721 TGGGCCCTGG GCTTCTACCC TGCGGAGATC ACACTGACCT GGCAGCGGGA TGGCGAGGAC
  781 CAAACTCAGG ACACCGAGCT TGTGGAGACC AGGCCAGCAG GAGATGGAAC CTTCCAGAAG
  841 TGGGCAGCTG TGGTGGTGCC TTCTGGAGAA GAGCAGAGAT ACACGTGCCA TGTGCAGCAC
  901 GAGGGGCTGC CAGAGCCCCT CACCCTGAGA TGGGAGCCAT CTTCCCAGCC CACCATCCCC
  961 ATCGTGGGCA TCGTTGCTGG GCCTGGCTGT CCTGGCTGTC CTAGCTGTCC TAGAGCTGTG
 1021 ATGGCTGTTG TGATGTGTAG GAGAGGAGCT CAGTGGAAAA GGAGGAGCTG CTCTCAGCTG
 1081 CGTCAGCCAC CAGTGCCAAG GCTCTGAATG AGTCTCTCAT TCGCATGTTA AAGCCTGGAG
1141 AACGCCGACC GACTCG
//
Primer: BGH_rev RDB13529_A5Iub.seq
>03377_13529_A5Iu_BGHrev_B02_05.ab1
    1 CAGTTTACGG GGTCTCTAGA CTCGAGCGGC CGCGTCTCAG GCTTTACAAG CGATGAGAGA
   61 CTCATCAGAG CCCTGGGCAC TGTTGCTGGA CGCAGCCTGA GAGCAGCTCC CTCCTTTTCC
  121 ACCTGAGCTC TTCCTCCTAC ACATCACAAC AGCCATCACA GCTCCTAGGA CAGCTAGGAC
  181 AGCCAGGACA GCCAGGCCAG CAACGATGCC CACGATGGGG ATGGTGGGCT GGGAAGATGG
  241 CTCCCATCTC AGGGTGAGGG GCTCTGGCAG CCCCTCGTGC TGCACATGGC ACGTGTATCT
  301 CTGCTCTTCT CCAGAAGGCA CCACCACAGC TGCCCACTTC TGGAAGGTTC CATCTCCTGC
  361 TGGCCTGGTC TCCACAAGCT CGGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT
  421 CAGTGTGATC TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC
  481 AGAGACGGGA TGGTGGGTCA CGTGTGTCTT TGGGTGTTCC GCGCGCTGCA GCGTCTCCTT
  541 CCCGTTCTCC AGGTATCTGC GGAGCCACTC CACGCACGTG CCCTCCAGGT AGGCTCTCCA
  601 CTGCTCCGCC TCACGGGCCG CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CCGTGTCCGC
  661 GGCGGTCCAG GAGCGCAGGT CCTCGTTCAG GGCGATGTAA TCCTTGCCGT CGTAGGCGGA
  721 CTGGTCATAC CCGCGGAGGA GGCGCCCGTC GGGCCCCAGG TCGCAGCCAT ACATCCACTG
  781 GAGGGTGTGA GACCCGTCCT CGCTCTGGTT GTAGTAGCCG CGCAGTTTCC GCAGGTTCAC
  841 TCGGTCAGCC TGTGCCTGGC GCTTGTACTT CTGTGTCTCC CGGTCCCAAT ACTCCGGCCC
  901 CTCCTGCTCC ACCCACGGCG CCCGGGGCTC CCCTCTCGGA CTCGCGGCGT CGCTGTCGAA
  961 CCCGCACGAA CTGCGTGTCG TCCACGTAGC CCACTGAGAT GAAGCGGGGC TCTCCGCGGG
 1021 CCGGGCCGGG ACACGGCGGT GTCGAAATAC CTCATGGGAG TGGGAGCAGG CCCAGGTCTC
 1081 GGTCAGGTCC AGGCTCCCCG AGAGCCAGCC AGGATGAAGG GATCGGACGC CAATGACCCC
1141 CGCAATCCTC CGGGCCGTTC TTGGGGGGAG AAAAAGAGCC CTTTTTAAAG T
//
Primer: SV40pro_F RDB13529_A5Iuc.seq
>03377_13529_A5Iu_SV40pro_F_C02_08.ab1
    1 CCCTCGGCTC CGGACATTCA GGAAGTAGGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG
  121 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
  181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
  241 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
  301 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
  361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
  421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
  481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
  541 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
  601 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC
  661 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
  721 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
  781 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
  841 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
  901 ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC
  961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC CGCCTTCTAT GAAAGGTTGG
 1021 GCTTCGGAAT CGTTTTCCGG GAACGCCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
 1081 GGCTGGAGGT TCTTTCGCCC ACCCCCAACT TGTTTAATTG CAGCTTATAA TTGTTTACAA
1141 ATTAAGGCAT AGCATTCACA ATGCCAAGTT AGCCATCCTT CATCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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