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Human HLA-C*06:02 cDNA

Human HLA-C*06:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03377
Resource name Human HLA-C*06:02 cDNA
Alternative name HLA-Cw*0602
Clone info. Human HLA-C*06:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0602 cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03377 Human HLA-C*06:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB03377z.seq
Electronic file Map RDB03377.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18740_B1C9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB18740_B1C9a.seq check
>03377_18740_B1C9_2_CMV-Forward_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT TTCTCCCCAG
  181 ACGCCGAGAT GCGGGTCATG GCGCCCCGAA CCCTCATCCT GCTGCTCTCG GGAGCCCTGG
  241 CCCTGACCGA GACCTGGGCC TGCTCCCACT CCATGAGGTA TTTCGACACC GCCGTGTCCC
  301 GGCCCGGCCG CGGAGAGCCC CGCTTCATCT CAGTGGGCTA CGTGGACGAC ACGCAGTTCG
  361 TGCGGTTCGA CAGCGACGCC GCGAGTCCGA GAGGGGAGCC CCGGGCGCCG TGGGTGGAGC
  421 AGGAGGGGCC GGAGTATTGG GACCGGGAGA CACAGAAGTA CAAGCGCCAG GCACAGGCTG
  481 ACCGAGTGAA CCTGCGGAAA CTGCGCGGCT ACTACAACCA GAGCGAGGAC GGGTCTCACA
  541 CCCTCCAGTG GANGTATGGC TGCGACCTNN NCCCGACGGG CGCCTCCTCC GCGGGTATGA
  601 CCAGTCCGCC TACGACGGCN AGGNNTACAT CGCCCTGAAC GANGANCTGC GCTCCNGGAN
  661 NGNCGCGGAN NNNGCGNCTN NNATNNCCNN NCGNNNGNGG NNNNNNCNNN GNNNNNNNNN
721 N
//
Primer: bGH_rev3
Region: bGHpA,insert 3'
Sequence file: RDB18740_B1C9b.seq check
>03377_18740_B1C9_2_bGH_rev3_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNGGNNG NNGNNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGACTCGA GCGGCCGCGT CTCAGGCTTT
  121 ACAAGCGATG AGAGACTCAT CAGAGCCCTG GGCACTGTTG CTGGACGCAG CCTGAGAGCA
  181 GCTCCCTCCT TTTCCACCTG AGCTCTTCCT CCTACACATC ACAACAGCCA TCACAGCTCC
  241 TAGGACAGCT AGGACAGCCA GGACAGCCAG GCCAGCAACG ATGCCCACGA TGGGGATGGT
  301 GGGCTGGGAA GATGGCTCCC ATCTCAGGGT GAGGGGCTCT GGCAGCCCCT CGTGCTGCAC
  361 ATGGCACGTG TATCTCTGCT CTTCTCCAGA AGGCACCACC ACAGCTGCCC ACTTCTGGAA
  421 GGTTCCATCT CCTGCTGGCC TGGTCTCCAC AAGCTCGGTG TCCTGAGTTT GGTCCTCGCC
  481 ATCCCGCTGC CAGGTCAGTG TGATCTCCGC AGGGTAGAAG CCCAGGGCCC AGCACCTCAG
  541 GGTGGCCTCA TGGTCAGAGA CGGGATGGTG GGTCACGTGT GTCTTTGGGT GTTCCGCGCG
  601 CTGCANCGTC TCCTTCCCGT TCTCCNGGTA TCTGCGGANC CACTCCACGC ACGTGCCCTN
  661 CANGTNNNNT CTNCNCNGCT NCNNNNCNNG GNNNGNNTNC NNCTNGCGCT GGGNGNNNNN
721 NNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, NeoR
Sequence file: RDB18740_B1C9c.seq check
>03377_18740_B1C9_2_SV40pro_ori_F_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNGCCGCC TCTGCCTCTG ANCTATTCCN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT ANGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
  121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
  181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
  241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
  301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
  361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
  421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
  481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
  541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
  601 GTCTTGTCGA TCANGANGAN CTGGANGAAN ANCATCNNGG GCTCGCGCCN GCCGAANNNN
  661 NNGCCNGGNT CNNNGNNCNN ATGNCCNANN NNANNNNCTC NTCNNGACCN NNNNNNANGN
721 NNGNNNGNNN ANNNNNNNGN NNNNAANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2021.03.31

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