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Human HLA-C*01:03 cDNA

Human HLA-C*01:03 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03376
Resource name Human HLA-C*01:03 cDNA
Alternative name HLA-Cw*0103
Clone info. Human HLA-C*01:03 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0103 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*0103

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB03376 Human HLA-C*01:03 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB03376z.seq
          Remarks, protocol and/or map (gif) RDB03376.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13721_A5Llp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp
          Sequence file: RDB13721_A5Lla.seq check
          >03376_13721_A5Ll_T7_20bp_B08_05.ab1
              1 CGTAACAGCG CTACTTAGCT TTTCTCCCCA GACGCCGAGA TGCGGGTCAT GGCGCCCCGA
             61 ACCCTCATCC TGCTGCTCTC GGGAGCCCTG GCCCTGACCG AGACCTGGGC CTGCTCCCAC
            121 TCCATGAAGT ATTTCTTCAC ATCCGTGTCC CGGCCTGGCC GCGGAGAGCC CCGCTTCATC
            181 TCAGTGGGCT ACGTGGACGA CACGCAGTTC GTGCGGTTCG ACAGCGACGC CGCGAGTCCG
            241 AGAGGGGAGC CGCGGGCGCC GTGGGTGGAG CAGGAGGGGC CGGAGTATTG GGACCGGGAG
            301 ACACAGAAGT ACAAGCGCCA GGCACAGACT GACCGAGTGA GCCTGCGGAA CCTGCGCGGC
            361 TACTACAACC AGAGCGAGGC CGGGTCTCAC ACCCTCCAGT GGATGTGTGG CTGCGACCTG
            421 GGGCCCGACG GGCGCCTCCT CCGCGGGTAT AACCAGTTCG CCTACGACGG CAAGGATTAC
            481 ATCGCCCTGA ACGAGGACCT GCGCTCCTGG ACCGCCGCGG ACACCGCGGC TCAGATCACC
            541 CAGCGCAAGT GGGAGGCGGC CCGTGAGGCG GAGCAGCGGA GAGCCTACCT GGAGGGCACG
            601 TGCGTGGAGT GGCTCCGCAG ATACCTGGAG AACGGGAAGG AGACGCTGCA GCGCGCGGAA
            661 CACCCAAAGA CACACGTGAC CCACCATCCC GTCTCTGACC ATGAGGCCAC CCTGAGGTGC
            721 TGGGCCCTGG GCTTCTACCC TGCGGAGATC ACACTGACCT GGGCAGTGGG ATGGGGGAGG
            781 ACCAAACTCA GGACACCGAG CTTGTGGAGA CCAGGCCAGC AGGAGATGGA AACCTTCCAG
            841 AAGTGGGCAG CTGTGATGGT GCCTTTCTGG AGAAGAGCAG AGATACACGT GCCATGTGCA
            901 GCACGAGGGG CTGCCCGGAG CCCCTCACCC TGAGATGGGA GGCGTCTTCC CAGCCCACCA
            961 ATCCCCATTC GTTGGGCCAT CGATGCCTGT CTTGTCCTGT CTTGTCTGTC TAGCTGTCCT
          1021 AGACCCTGTG ATTGGCTGGG TGTGCATGGT GTGTTACG
          //
          Primer: BGH_rev
          Sequence file: RDB13721_A5Llb.seq check
          >03376_13721_A5Ll_T7_BGHrev_A05_02.ab1
              1 CGTTTAACCG GGGCCCTCCT AGAACTCGAG CGGCCGCGGT CTCAGGCTTT ACAAGCGATG
             61 AGAGACTCAT CAGAGCCCTG GGCACTGGTT GCTGGACGCA GCCTGAGAGC AGCTCCCTCC
            121 TTTTCCACCT GAGCTCTTCC TCCTACACAT CACAACAGCC ACCACAGCTC CTAGGACAGC
            181 TAGGACAGCC AGGACAGCCA GGCCAGCAAC GATGCCCACG ATGGGGATGG TGGGCTGGGA
            241 AGACGGCTCC CATCTCAGGG TGAGGGGCTC CGGCAGCCCC TCGTGCTGCA CATGGCACGT
            301 GTATCTCTGC TCTTCTCCAG AAGGCACCAT CACAGCTGCC CACTTCTGGA AGGTTCCATC
            361 TCCTGCTGGC CTGGTCTCCA CAAGCTCGGT GTCCTGAGTT TGGTCCTCCC CATCCCACTG
            421 CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC CAGCACCTCA GGGTGGCCTC
            481 ATGGTCAGAG ACGGGATGGT GGGTCACGTG TGTCTTTGGG TGTTCCGCGC GCTGCAGCGT
            541 CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT CCAGGTAGGC
            601 TCTCCGCTGC TCCGCCTCAC GGGCCGCCTC CCACTTGCGC TGGGTGATCT GAGCCGCGGT
            661 GTCCGCGGCG GTCCAGGAGC GCAGGTCCTC GTTCAGGGCG ATGTAATCCT TGCCGTCGTA
            721 GGCGAACTGG TTATACCCGC GGAGGAGGCG CCCGTCGGGG CCCCAGGTCG CAGCCAACAC
            781 ATCCACTGGA GGGTGTGAGA CCCGGCCTCG CTCTGGTTGT AGTAGCCGCG CAGGTTTCCG
            841 CAGGCTCACT CGGTCAGTCT GTGCCCTGGC GCTTGTACTT CTGTGTCTCC CGGTCCCAAT
            901 ACTCCCGGCC CCTTCCTGCT CCACCCCACG GCGGCCCGCG GCTCCCCTCT CGGGACTCGC
            961 GGCGTCGCTT GTCGAAACCG CACGAACTGC GTGTCGTCCA CGTAGCCCAC TGAGATGAAG
           1021 CCGGGGCTTC TTCCGCGGCC AGGCCGGACA CGGATAGTGA AAGAAATAAC TTCATGAAAT
           1081 GGGAAGCAGG CCCAAAGTCC TCCGGGTCAG GTCCAAGGCT CCTGAGATAG CCAGCAAAGG
           1141 AATGAGGGAT CCGGGCAGCA TGGACCCGGC AATTCTCTGG ACGTTCCTGG GGAGAAGAAA
          1201 AAGAGACTAC TTAAGATTA
          //
          Primer: SV40pro_F
          Sequence file: RDB13721_A5Llc.seq check
          >03376_13721_A5Ll_SV40pro_F_D08_11.ab1
              1 CTCGGTCTGG GACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
            121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
            661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GGACCGCTAT
            781 CAGACATAGC GTTTGGCTAC CCGTGATATT GCTGAGAGCT TGGCGGCGAA TGGGCTGACC
            841 GCTTTCTCGT GCTTTACGGT ATCGCCGCTC CCGATTTCGC AGCGCATCGC TTCTATCGCC
            901 TTCTTGACCG AGTCCTCTGG ATGGACTCTG GGGATCGGAA TTGACGACAG CGAACGCCCA
            961 ACCTGGCATC ACGAGAATTC GAATTACACG CTCGCATCTT ATGGATGTGG CATCGAATCG
          1021 ATACGGACCG GCTGGATGAT TCATCCAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl