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Human HLA-C*01:03 cDNA

Human HLA-C*01:03 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03376
Resource name Human HLA-C*01:03 cDNA
Alternative name HLA-Cw*0103
Clone info. Human HLA-C*01:03 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0103 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03376 Human HLA-C*01:03 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03376z.seq from Depositor
Electronic file Map RDB03376.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13721_A5Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13721_A5Lla.seq
>03376_13721_A5Ll_T7_20bp_B08_05.ab1
    1 CGTAACAGCG CTACTTAGCT TTTCTCCCCA GACGCCGAGA TGCGGGTCAT GGCGCCCCGA
   61 ACCCTCATCC TGCTGCTCTC GGGAGCCCTG GCCCTGACCG AGACCTGGGC CTGCTCCCAC
  121 TCCATGAAGT ATTTCTTCAC ATCCGTGTCC CGGCCTGGCC GCGGAGAGCC CCGCTTCATC
  181 TCAGTGGGCT ACGTGGACGA CACGCAGTTC GTGCGGTTCG ACAGCGACGC CGCGAGTCCG
  241 AGAGGGGAGC CGCGGGCGCC GTGGGTGGAG CAGGAGGGGC CGGAGTATTG GGACCGGGAG
  301 ACACAGAAGT ACAAGCGCCA GGCACAGACT GACCGAGTGA GCCTGCGGAA CCTGCGCGGC
  361 TACTACAACC AGAGCGAGGC CGGGTCTCAC ACCCTCCAGT GGATGTGTGG CTGCGACCTG
  421 GGGCCCGACG GGCGCCTCCT CCGCGGGTAT AACCAGTTCG CCTACGACGG CAAGGATTAC
  481 ATCGCCCTGA ACGAGGACCT GCGCTCCTGG ACCGCCGCGG ACACCGCGGC TCAGATCACC
  541 CAGCGCAAGT GGGAGGCGGC CCGTGAGGCG GAGCAGCGGA GAGCCTACCT GGAGGGCACG
  601 TGCGTGGAGT GGCTCCGCAG ATACCTGGAG AACGGGAAGG AGACGCTGCA GCGCGCGGAA
  661 CACCCAAAGA CACACGTGAC CCACCATCCC GTCTCTGACC ATGAGGCCAC CCTGAGGTGC
  721 TGGGCCCTGG GCTTCTACCC TGCGGAGATC ACACTGACCT GGGCAGTGGG ATGGGGGAGG
  781 ACCAAACTCA GGACACCGAG CTTGTGGAGA CCAGGCCAGC AGGAGATGGA AACCTTCCAG
  841 AAGTGGGCAG CTGTGATGGT GCCTTTCTGG AGAAGAGCAG AGATACACGT GCCATGTGCA
  901 GCACGAGGGG CTGCCCGGAG CCCCTCACCC TGAGATGGGA GGCGTCTTCC CAGCCCACCA
  961 ATCCCCATTC GTTGGGCCAT CGATGCCTGT CTTGTCCTGT CTTGTCTGTC TAGCTGTCCT
1021 AGACCCTGTG ATTGGCTGGG TGTGCATGGT GTGTTACG
//
Primer: BGH_rev RDB13721_A5Llb.seq
>03376_13721_A5Ll_T7_BGHrev_A05_02.ab1
    1 CGTTTAACCG GGGCCCTCCT AGAACTCGAG CGGCCGCGGT CTCAGGCTTT ACAAGCGATG
   61 AGAGACTCAT CAGAGCCCTG GGCACTGGTT GCTGGACGCA GCCTGAGAGC AGCTCCCTCC
  121 TTTTCCACCT GAGCTCTTCC TCCTACACAT CACAACAGCC ACCACAGCTC CTAGGACAGC
  181 TAGGACAGCC AGGACAGCCA GGCCAGCAAC GATGCCCACG ATGGGGATGG TGGGCTGGGA
  241 AGACGGCTCC CATCTCAGGG TGAGGGGCTC CGGCAGCCCC TCGTGCTGCA CATGGCACGT
  301 GTATCTCTGC TCTTCTCCAG AAGGCACCAT CACAGCTGCC CACTTCTGGA AGGTTCCATC
  361 TCCTGCTGGC CTGGTCTCCA CAAGCTCGGT GTCCTGAGTT TGGTCCTCCC CATCCCACTG
  421 CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC CAGCACCTCA GGGTGGCCTC
  481 ATGGTCAGAG ACGGGATGGT GGGTCACGTG TGTCTTTGGG TGTTCCGCGC GCTGCAGCGT
  541 CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT CCAGGTAGGC
  601 TCTCCGCTGC TCCGCCTCAC GGGCCGCCTC CCACTTGCGC TGGGTGATCT GAGCCGCGGT
  661 GTCCGCGGCG GTCCAGGAGC GCAGGTCCTC GTTCAGGGCG ATGTAATCCT TGCCGTCGTA
  721 GGCGAACTGG TTATACCCGC GGAGGAGGCG CCCGTCGGGG CCCCAGGTCG CAGCCAACAC
  781 ATCCACTGGA GGGTGTGAGA CCCGGCCTCG CTCTGGTTGT AGTAGCCGCG CAGGTTTCCG
  841 CAGGCTCACT CGGTCAGTCT GTGCCCTGGC GCTTGTACTT CTGTGTCTCC CGGTCCCAAT
  901 ACTCCCGGCC CCTTCCTGCT CCACCCCACG GCGGCCCGCG GCTCCCCTCT CGGGACTCGC
  961 GGCGTCGCTT GTCGAAACCG CACGAACTGC GTGTCGTCCA CGTAGCCCAC TGAGATGAAG
 1021 CCGGGGCTTC TTCCGCGGCC AGGCCGGACA CGGATAGTGA AAGAAATAAC TTCATGAAAT
 1081 GGGAAGCAGG CCCAAAGTCC TCCGGGTCAG GTCCAAGGCT CCTGAGATAG CCAGCAAAGG
 1141 AATGAGGGAT CCGGGCAGCA TGGACCCGGC AATTCTCTGG ACGTTCCTGG GGAGAAGAAA
1201 AAGAGACTAC TTAAGATTA
//
Primer: SV40pro_F RDB13721_A5Llc.seq
>03376_13721_A5Ll_SV40pro_F_D08_11.ab1
    1 CTCGGTCTGG GACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GGACCGCTAT
  781 CAGACATAGC GTTTGGCTAC CCGTGATATT GCTGAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTTCTCGT GCTTTACGGT ATCGCCGCTC CCGATTTCGC AGCGCATCGC TTCTATCGCC
  901 TTCTTGACCG AGTCCTCTGG ATGGACTCTG GGGATCGGAA TTGACGACAG CGAACGCCCA
  961 ACCTGGCATC ACGAGAATTC GAATTACACG CTCGCATCTT ATGGATGTGG CATCGAATCG
1021 ATACGGACCG GCTGGATGAT TCATCCAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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