Resource data sheet
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Human HLA-B*59:01 cDNA

Human HLA-B*59:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03375
Resource name Human HLA-B*59:01 cDNA
Alternative name HLA-B*5901
Clone info. Human HLA-B*59:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03375 Human HLA-B*59:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03375z.seq from Depositor
Electronic file Map RDB03375.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB03375_A2Kip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_long RDB03375_A2Kia.seq
>03375_03375_A2Ki_T7Long_C09_09.ab1
    1 CAAAGGCCCT GGGCTAGCGT TTAACTTAAG CTTCACCCGG ACTCAGAATC TCCTCAGACA
   61 CCGAGATGCG GGTCACGGCA CCCCGAACCC TCCTCCTGCT GCTCTGGGGG GCCCTGGCCC
  121 TGACCGAGAC CTGGGCCGGC TCCCACTCCA TGAGGTATTT CTACACCGCC ATGTCCCGGC
  181 CCGGCCGCGG GGAGCCCCGC TTCATCGCAG TGGGCTACGT GGACGACACG CAGTTCGTGA
  241 GGTTCGACAG CGACGCCGCG AGTCCGAGAG AGGAGCCGCG GGCGCCGTGG ATAGAGCAGG
  301 AGGGGCCGGA GTATTGGGAC CGGAACACAC AGATCTTCAA GACCAACACA CAGACTTACC
  361 GAGAGAACCT GCGGATCGCG CTCCGCTACT ACAACCAGAG CGAGGCCGGG TCTCACACTT
  421 GGCAGACGAT GTATGGCTGC GACCTGGGGC CGGACGGGCG CCTCCTCCGC GGGCATAACC
  481 AGTTAGCCTA CGACGGCAAG GATTACATCG CCCTGAACGA GGACCTGAGC TCCTGGACCG
  541 CGGCGGACAC CGCGGCTCAG ATCACCCAGC GCAAGTGGGA GGCGGCCCGT GTGGCGGAGC
  601 AGCTGAGAGC CTACCTGGAG GGCACGTGCG TGGAGTGGCT CCGCAGATAC CTGGAGAACG
  661 GGAAGGAGAC GCTGCAGCGC GCGGACCCCC CAAAGACACA CGTGACCCAC CACCCCATCT
  721 CTGACCATGA GGCCACCCTG AGGTGCTGGG CCCTGGGCTT CTACCCTGCG GAGATCACAC
  781 TGACCTGGCA GCGGGATGGC GAGGACCAAA CTCAGGACAC TGAGCTTGTG GAGACCAGAC
  841 CAGCAGGAGA TAGAACCTTC CAGAAGTGGG CAGCTGTGGT GGTGCCTTCT GGAGAAGAGC
  901 AGAGATACAC ATGCCATGTA CAGCATGAGG GGCTGCCGAA GCCCCTCACC CTGAGATGGG
  961 AGCCATCTTC CCAGTCCACC ATCCCCATCG TGGGCATTGT TGCTGGGCCT GGCTGTCCTA
 1021 GCAGTTGTGG TCATCGGAGC TGTGGTCGCT ACTGTGATGT GTAGAGGAAG AAGCTCAGTG
 1081 GAAAAGAGTA GCTACTCTCA GCTGCGTCCA GCGACAGTGC CAGGCTCTGA TGTGTTCTTC
1141 TCACCAAGCC TTTGAAAAAA GTCCTTGGAA GAGATATAGT
//
Primer: BGH_rev RDB03375_A2Kib.seq
>03375_03375_A2Ki_BGH_rev_D09_12.ab1
    1 CAGTTACGGG GGCTCTAGAC TCGAGCGGCC GCATCTCAGT CCCTCACAAG ACAGCTGTCT
   61 CAGGCTTTTC AAGCTGTGAG AGACACATCA GAGCCCTGGG CACTGTCGCT GGACGCAGCC
  121 TGAGAGTAGC TCCCTCCTTT TCCACCTGAG CTCTTCCTCC TACACATCAC AGTAGCGACC
  181 ACAGCTCCGA TGACCACAAC TGCTAGGACA GCCAGGCCAG CAACAATGCC CACGATGGGG
  241 ATGGTGGACT GGGAAGATGG CTCCCATCTC AGGGTGAGGG GCTTCGGCAG CCCCTCATGC
  301 TGTACATGGC ATGTGTATCT CTGCTCTTCT CCAGAAGGCA CCACCACAGC TGCCCACTTC
  361 TGGAAGGTTC TATCTCCTGC TGGTCTGGTC TCCACAAGCT CAGTGTCCTG AGTTTGGTCC
  421 TCGCCATCCC GCTGCCAGGT CAGTGTGATC TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC
  481 CTCAGGGTGG CCTCATGGTC AGAGATGGGG TGGTGGGTCA CGTGTGTCTT TGGGGGGTCC
  541 GCGCGCTGCA GCGTCTCCTT CCCGTTCTCC AGGTATCTGC GGAGCCACTC CACGCACGTG
  601 CCCTCCAGGT AGGCTCTCAG CTGCTCCGCC ACACGGGCCG CCTCCCACTT GCGCTGGGTG
  661 ATCTGAGCCG CGGTGTCCGC CGCGGTCCAG GAGCTCAGGT CCTCGTTCAG GGCGATGTAA
  721 TCCTTGCCGT CGTAGGCTAA CTGGTTATGC CCGCGGAGGA GGCGCCCGTC CGGCCCCAGG
  781 TCGCAGCCAT ACATCGTCTG CCAAGTGTGA GACCCGGCCT CGCTCTGGTT GTAGTAGCGG
  841 AGCGCGATCC GCAGGTTCTC TCGGTAAGTC TGTGTGTTGG TCTTGAAGAT CTGTGTGTTC
  901 CGGTCCCAAT ACTCCGGCCC CTCCTGCTCT ATCCACGGCG CCCGCGGCTC CTCTCTCGGA
  961 CTCGCGGCGT CGCTGTCGAA CCTCACGAAC TGCGTGTCGT CCACGTAGCC CACTGCGATG
 1021 AAGCGGGGCT CCCCGCGGGC CGGGCCGGGG ACATGCGGTG TAGAAAATAC CTCATGGAGT
 1081 GGGGAGCCGC CAGGTCTCGG TCAGGACCAA GGACCCCAGA GCCAGCAAGG AGGAGGTTTC
1141 GGGGTGCCGT GACCCGCCAA TTCTTCGGGT GTTCTTGTAG
//
Primer: SV40pro_F RDB03375_A2Kic.seq
>03375_03375_A2Ki_SV40pro_F_H04_22.ab1
    1 ACCTGGTCCG TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  961 CCCAACCCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
 1021 CGGGATCGTT TTCCGGGACG CCGGGCTGGA ATGATCTCAG CGCGGGGATC TCATGCTGAA
 1081 GTCCTCGCCA ACCGACTGTT ATGCAGCTAT ATGTTAGAAT AGGCCATAGC ATCCACAATT
1141 TTCACGAGTT AGGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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