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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-B*59:01 cDNA

Human HLA-B*59:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03375
Resource name Human HLA-B*59:01 cDNA
Alternative name HLA-B*5901
Clone info. Human HLA-B*59:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03375 Human HLA-B*59:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB03375z.seq
Electronic file Map RDB03375.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13980_A6C3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB13980_A6C3d.seq check
>03375_13980_B0GgF_CMV-Forward_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNNNN GCNNTGGGCG GTANGCGTGT ACGGNGGGNG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
  181 ATCTCCTCAG ACACCGAGAT GCGGGTCACG GCACCCCGAA CCCTCCTCCT GCTGCTCTGG
  241 GGGGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
  301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA CGTGGACGAC
  361 ACGCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GAGAGGAGCC GCGGGCGCCG
  421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGAACA CACAGATCTT CAAGACCAAC
  481 ACACAGACTT ACCGAGAGAA CCTGCGGATC GCGCTCCGCT ACTACAACCA GAGCGAGGCC
  541 GGGTCTCACA CTTGGCAGAC GATGTATGGC TGCGACCTGG GGCCGGACGG GCGCCTCCTC
  601 CGCGGGCATA ACCAGTTAGC CTACGACGGC AAGGNNTACA TCGCCCTGAA CGANGACCTG
  661 ANCTCCTGGA NCGCGGCGGA CACCGCGGCT CANATCACCC AGCGCAAGTG GGANGCGGCN
  721 CGNGTGGCGG ANCAGCTNAN AGCCTACCTG GNNGGCACGN GCNNGGNNTG GCTCCNCANA
  781 NACCNNGNNA ACGGGAANGA NNNGCTNCAN CGCGNNGNNC CCCCAAANNN ACNCNNNNNC
  841 NNCCNCCCCN TCTNNNANCN TNNNNNNNCC NNNANGNGCT GGNNCNNNGN NNTNNNACCN
901 NNNNNNNNN
//
Primer: BGH_rev2
Region: bGH pA, insert 3'
Sequence file: RDB13980_A6C3e.seq check
>03375_13980_B0GgF_BGH_rev2_B09_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNG GNNGGGCNNN CAACANNTGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC ATCTCAGTCC
  121 CTCACAAGAC AGCTGTCTCA GGCTTTTCAA GCTGTGAGAG ACACATCAGA GCCCTGGGCA
  181 CTGTCGCTGG ACGCAGCCTG AGAGTAGCTC CCTCCTTTTC CACCTGAGCT CTTCCTCCTA
  241 CACATCACAG TAGCGACCAC AGCTCCGATG ACCACAACTG CTAGGACAGC CAGGCCAGCA
  301 ACAATGCCCA CGATGGGGAT GGTGGACTGG GAAGATGGCT CCCATCTCAG GGTGAGGGGC
  361 TTCGGCAGCC CCTCATGCTG TACATGGCAT GTGTATCTCT GCTCTTCTCC AGAAGGCACC
  421 ACCACAGCTG CCCACTTCTG GAAGGTTCTA TCTCCTGCTG GTCTGGTCTC CACAAGCTCA
  481 GTGTCCTGAG TTTGGTCCTC GCCATCCCGC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG
  541 AAGCCCAGGG CCCAGCACCT CAGGGTGGCC TCATGGTCAG AGATGGGGTG GTGGGTCACG
  601 TGTGTCTTTG GGGGGTCCGC GCGCTGCAGC GTCTCCTTCC CGTTCTCCAG GTATCTGCGG
  661 ANCCACTCCA CGCACGTGCC CTCCAGGTAN GCTCTCAGCT GCTCCGCCAC ACGGGCCGCC
  721 TCCCACTTGC GCTGGGTGNN CTGANCCGCG GNGTCCGCCG CGGNCCANGA NCTCNNGTCC
  781 TCGTTCANGG CGATGTAATC CTTGCCGNCN NNNNNAACNN GTTATGCCCG CGGNNGANGC
841 GCCNGNNNGG NCCCANGNCG CANCCNTACN TCNNNNGCNN NNNNNNNN
//
Primer: SV40pro_ori_f
Region: SV40 pro_ori, NeoR
Sequence file: RDB13980_A6C3f.seq check
>03375_13980_B0GgF_SV40pro_ori_F_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNGCCGCCT CTGCCTCTNN NCTATTCNNG AAGTAGTGAG GANGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
  121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
  541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
  601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
  661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGNTCTC GTCGTGACCC ATGGCGATGC
  721 CTGCTTGCCG AATATCATGG TGGAAAAATG GCCGCTTTTC TGGATTCATC GACNGTGGCC
  781 GGCNGGGNGT GGCGGACCGC TATCANGNCA TANCGTTGGC TANCCGNNAT ATNGCTGAAG
  841 ANCTNGGCNG CNAANGGNCN NANCGCNNNC TCNNGCTTTA CNNNATCGNC NCNNCNNNNN
901 CNCANCNNNN CNNCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl