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Human HLA-B*55:02 cDNA

Human HLA-B*55:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03374
Resource name Human HLA-B*55:02 cDNA
Alternative name HLA-B*5502
Clone info. Human HLA-B*55:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03374 Human HLA-B*55:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB03374z.seq

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18371_B0Gup1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: cCMV pro,insert 5'
Sequence file: RDB18371_B0Gua.seq check
>03374_18371_B0Gu_1_CMV-Forward_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNCNNTTGN NGCNNTGGGC GGTANGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTCAC CCGGACTCAG
  181 AATCTCCTCA GACACCGAGA TGCGGGTCAC GGCACCCCGA ACCCTCCTCC TGCTGCTCTG
  241 GGGGGCCCTG GCCCTGACCG AGACCTGGGC CGGCTCCCAC TCCATGAGGT ATTTCTACAC
  301 CGCCATGTCC CGGCCCGGCC GCGGGGAGCC CCGCTTCATC GCAGTGGGCT ACGTGGACGA
  361 CACGCAGTTC GTGAGGTTCG ACAGCGACGC CGCGAGTCCG AGAGAGGAGC CGCGGGCGCC
  421 GTGGATAGAG CAGGAGGGGC CGGAGTATTG GGACCGGAAC ACACAGATCT ACAAGGCCCA
  481 GGCACAGACT GACCGAGAGA GCCTGCGGAA CCTGCGCGGC TACTACAACC AGAGCGAGGC
  541 CGGGTCTCAC ACTTGGCAGA CGATGTATGG CTGCGACCTG GGGCCGGACG GGCGCCTCCT
  601 CCGCGGGCAT AACCAGTTAG CCTACGACGG CAAGGANTAC ATCGCCCTGA ACGAGGACCT
  661 GAGCTCCTGG ACCGCGGCGG ACACCGCGGC TCAGATCACC CAGCGCAAGT GGGAGGCGGC
  721 CCGTGTGGCG GANCAGCTGA NAGCCTACCT GGAGGGCACG TGCGTGGAGT GGCTCCGCAG
  781 ATACCTGGAG AANGGGAAGG ANANGCTGCA GCGCGCGGAN CCCCCAAAGA CACACGTGAC
  841 CCACCACCCC NTCTCTGACC NTGANGCCAC CCTGANGTGN TGGGCCCNGG GNTTCTANCC
  901 TGCGGANATC NCACNNNNCN NNNNGCGGGN NGGCGANGAN NNAACTCNNN ANNCTGANCT
  961 TGNGNANANC AGANNAGCNN NANANNGAAN CNNTNCNNAN GNGGGCAGCN NNGGNNNNNN
1021 CNNNNNNNNN ANNNNNNNNN N
//
Primer: BGH_rev2
Region: bGHpA,insert 3'
Sequence file: RDB18371_B0Gub.seq check
>03374_18371_B0Gu_1_BGH_rev2_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGG GGNNGGGNNN ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CATCTCAGTC
  121 CCTCACAAGA CAGCTGTCTC AGGCTTTTCA AGCTGTGAGA GACACATCAG AGCCCTGGGC
  181 ACTGTCGCTG GACGCAGCCT GAGAGTAGCT CCCTCCTTTT CCACCTGAGC TCTTCCTCCT
  241 ACACATCACA GTAGCGACCA CAGCTCCGAT GACCACAACT GCTAGGACAG CCAGGCCAGC
  301 AACAATGCCC ACGATGGGGA TGGTGGACTG GGAAGATGGC TCCCATCTCA GGGTGAGGGG
  361 CTTCGGCAGC CCCTCATGCT GTACATGGCA TGTGTATCTC TGCTCTTCTC CAGAAGGCAC
  421 CACCACAGCT GCCCACTTCT GGAAGGTTCT ATCTCCTGCT GGTCTGGTCT CCACAAGCTC
  481 AGTGTCCTGA GTTTGGTCCT CGCCATCCCG CTGCCAGGTC AGTGTGATCT CCGCAGGGTA
  541 GAAGCCCAGG GCCCAGCACC TCAGGGTGGC CTCATGGTCA GAGATGGGGT GGTGGGTCAC
  601 GTGTGTCTTT GGGGGGTCCG CGCGCTGCAG CGTCTCCTTC CCGTTCTCCA GGTATCTGCG
  661 GAGCCACTCC ACGCACGTGC CCTCCAGGTA GGCTCTCAGC TGCTCCGCCA CACGGGCCGC
  721 CTCCCACTTG CGCTGGGTGA NCTGAGCCGC GNTGTCCGCC GCGGTCCANG ANCTCANGTC
  781 CTCGTTCANG GCGATGTAAT CCTTGCCGTC GTNNNTAACT GGTTATGCCC GCGGANGANG
  841 CGCCCGNNCG GNCCCAGGNN GCANCCATAC NTCGTCTGNC NAGNGTGANA NCCNGNCTCG
  901 NNNTNNTNGN ANTANCCGCG NNNNNTNCNN NNNNNTNNNG GNCAGNCTGN NNNNNGNNNN
961 NNNNNN
//
Primer: SV40pro_ori_f
Region: SV40 pro_ori,NeoR
Sequence file: RDB18371_B0Guc.seq check
>03374_18371_B0Gu_1_SV40pro_ori_F_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC CTCTGCCTCT GAGCTNTTCN NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
  121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
  181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
  241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
  301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
  361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
  421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
  481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
  541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
  601 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
  661 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGANGATC TCGTCGTGAC CCATGGCGAT
  721 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
  781 CGGCTGGGTG TGGCGGANCG CTATCANGAC ATAGCGTTGG CNNNNNNGAN ATTGCTGAAG
  841 ANCTTGGCGG CGAANGGGCT GANCGCTTCC TCNNGCTTTA CGGTATCGCC GCTCCCGANN
  901 CNCANCGCAT CGCCTTCTAT CNNCNTCNNN NNNANTNCTT NNGANCGGGN NNNNGGGNNN
961 CNNANTGANN GANNNNNCGA NNCCNNNNCN NNNNNTCNCN ANANTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.09.02

GNP_filter3_RDBDEP_html_200316.pl