RDB03374 - Human HLA-B*55:02 cDNA

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Human HLA-B*55:02 cDNA

Human HLA-B*55:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number	RDB03374
Resource name	Human HLA-B*55:02 cDNA
Alternative name	HLA-B*5502
Clone info.	Human HLA-B*55:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human MHC class I B cDNA
Depositor\|Developer	Akatsuka, Yoshiki \|

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human MHC class I B

External Database
Human MHC class I B

Reference sequence

Sequence (full)	RDB03374zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB03374	Human HLA-B*55:02 cDNA	DNA solution

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The Human HLA-B*55:02 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03374).

Reference section:

Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18371_B0Gup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB18371_B0Gud.seq
>03374_18371_B2L7F_CMV_Forward_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NANNNNNGNN NNGNGNNNAN 61 GNNTNNNNNN GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA 121 ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC 181 CGGACTCAGA ATCTCCTCAG ACACCGAGAT GCGGGTCACG GCACCCCGAA CCCTCCTCCT 241 GCTGCTCTGG GGGGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA 301 TTTCTACACC GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA 361 CGTGGACGAC ACGCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GAGAGGAGCC 421 GCGGGCGCCG TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGAACA CACAGATCTA 481 CAAGGCCCAG GCACAGACTG ACCGAGAGAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA 541 GAGCGAGGCC GGGTCTCACA CTTGGCAGAC GATGTATGGC TGCGACCTGG GGCCGGACGG 601 GCGCCTCCTC CGCGGGCATA ACCAGTTAGC CTACGACGGC AAGGATTACA TCGCCCTGAA 661 CGAGGACCTG AGCTCCTGGA CCGCGGCGGA CACCGCGGCT CAGATCACCC AGCGCAAGTG 721 GGAGGCGGNC CGTGTGGCGG ANCAGCTGAG AGCCTACCTG GAGGGCACGT GCGTGGAGTG 781 GCTCCGCAGA TACCTGGAGA ACGGGAAGGA GANGCTGCAN CGCGCGGANC CCCCNAAGAC 841 ACACGTGACC CACCACCCCN TCTCTGANCA TGANGNCNNC CTNNNGTGCT GGNCCCNGGG 901 GCNNNTACCC NGCGGNNNTN ACACNGACNN NGNNGCNGGN TNGGNGANGN ANCNAANNTC 961 ANNANACTNA NCNTNNNGGA NANNANANNN NCNNGNNNNN NNANNNNNNN CNNNNNNNGN 1021 NNNNNNNNGN NNNGNNNNNN NNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,insert 3' Sequence file: RDB18371_B0Gue.seq
>03374_18371_B0Gu_1_BGH_rev2_B04_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGGG GGNNGGGNNN ACAACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CATCTCAGTC 121 CCTCACAAGA CAGCTGTCTC AGGCTTTTCA AGCTGTGAGA GACACATCAG AGCCCTGGGC 181 ACTGTCGCTG GACGCAGCCT GAGAGTAGCT CCCTCCTTTT CCACCTGAGC TCTTCCTCCT 241 ACACATCACA GTAGCGACCA CAGCTCCGAT GACCACAACT GCTAGGACAG CCAGGCCAGC 301 AACAATGCCC ACGATGGGGA TGGTGGACTG GGAAGATGGC TCCCATCTCA GGGTGAGGGG 361 CTTCGGCAGC CCCTCATGCT GTACATGGCA TGTGTATCTC TGCTCTTCTC CAGAAGGCAC 421 CACCACAGCT GCCCACTTCT GGAAGGTTCT ATCTCCTGCT GGTCTGGTCT CCACAAGCTC 481 AGTGTCCTGA GTTTGGTCCT CGCCATCCCG CTGCCAGGTC AGTGTGATCT CCGCAGGGTA 541 GAAGCCCAGG GCCCAGCACC TCAGGGTGGC CTCATGGTCA GAGATGGGGT GGTGGGTCAC 601 GTGTGTCTTT GGGGGGTCCG CGCGCTGCAG CGTCTCCTTC CCGTTCTCCA GGTATCTGCG 661 GAGCCACTCC ACGCACGTGC CCTCCAGGTA GGCTCTCAGC TGCTCCGCCA CACGGGCCGC 721 CTCCCACTTG CGCTGGGTGA NCTGAGCCGC GNTGTCCGCC GCGGTCCANG ANCTCANGTC 781 CTCGTTCANG GCGATGTAAT CCTTGCCGTC GTNNNTAACT GGTTATGCCC GCGGANGANG 841 CGCCCGNNCG GNCCCAGGNN GCANCCATAC NTCGTCTGNC NAGNGTGANA NCCNGNCTCG 901 NNNTNNTNGN ANTANCCGCG NNNNNTNCNN NNNNNTNNNG GNCAGNCTGN NNNNNGNNNN 961 NNNNNN //
Primer: SV40pro_ori_f (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB18371_B0Guf.seq
>03374_18371_B0Gu_1_SV40pro_ori_F_C04_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC CTCTGCCTCT GAGCTNTTCN NGAAGTAGTG AGGAGGCTTT 61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 601 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 661 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGANGATC TCGTCGTGAC CCATGGCGAT 721 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 781 CGGCTGGGTG TGGCGGANCG CTATCANGAC ATAGCGTTGG CNNNNNNGAN ATTGCTGAAG 841 ANCTTGGCGG CGAANGGGCT GANCGCTTCC TCNNGCTTTA CGGTATCGCC GCTCCCGANN 901 CNCANCGCAT CGCCTTCTAT CNNCNTCNNN NNNANTNCTT NNGANCGGGN NNNNGGGNNN 961 CNNANTGANN GANNNNNCGA NNCCNNNNCN NNNNNTCNCN ANANTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

2024.03.26

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