Resource data sheet
Prev.

Human HLA-B*55:02 cDNA

Human HLA-B*55:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03374
Resource name Human HLA-B*55:02 cDNA
Alternative name HLA-B*5502
Clone info. Human HLA-B*55:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03374 Human HLA-B*55:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03374z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13687_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13687_A5Jga.seq
>03374_13687_A5Jg_CMV-Forward_A09_03.ab1
    1 AGTTAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
  181 ATCTCCTCAG ACACCGAGAT GCGGGTCACG GCACCCCGAA CCCTCCTCCT GCTGCTCTGG
  241 GGGGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
  301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA CGTGGACGAC
  361 ACGCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GAGAGGAGCC GCGGGCGCCG
  421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGAACA CACAGATCTA CAAGGCCCAG
  481 GCACAGACTG ACCGAGAGAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA GAGCGAGGCC
  541 GGGTCTCACA CTTGGCAGAC GATGTATGGC TGCGACCTGG GGCCGGACGG GCGCCTCCTC
  601 CGCGGGCATA ACCAGTTAGC CTACGACGGC AAGGATTACA TCGCCCTGAA CGAGGACCTG
  661 AGCTCCTGGA CCGCGGCGGA CACCGCGGCT CAGATCACCC AGCGCAAGTG GGAGGCGGCC
  721 CGTGTGGCGG AGCAGCTGAG AGCCTACCTG GAGGGCACGT GCGTGGAGTG GCTCCGCAGA
  781 TACCTGGAGA ACGGGAAGGA GACGCTGCAG CGCGCGGACC CCCCAAAGAC ACACGTGACC
  841 CACCACCCCA TCTCTGACCA TGAGGCCACC CTGAGGTGCT GGGCCCTGGG CTTCTACCCT
  901 GCGGAGATCA CACTGACCTG GCAGCGGGAT GGCGAGGACC AAACTCAGGA CACTGAGCTT
  961 GTGGAGACCA GACCAGCAGG AGATAGAACC TTCCAGAAGT GGGCAGCTGT GGTGGTGCCC
 1021 TTCTGGGAGA AGAGCAGAGA TACACATGCC CATGTTACAG CATGAGGGGC TGCCGAAGCC
 1081 CCTTCACCTG AGATGGGCAG ACATCCTTCC CAGGTTCCAC CCATTCCCCC TCATTCGAAG
//
Primer: BGH_rev RDB13687_A5Jgb.seq
>03374_13687_A5Jg_BGHrev_G06_21.ab1
    1 GTTCGGGACT CTAGACTCGA GCGGCCGCAT CTCAGTCCCT CACAAGACAG CTGTCTCAGG
   61 CTTTTCAAGC TGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCGCTGGAC GCAGCCTGAG
  121 AGTAGCTCCC TCCTTTTCCA CCTGAGCTCT TCCTCCTACA CATCACAGTA GCGACCACAG
  181 CTCCGATGAC CACAACTGCT AGGACAGCCA GGCCAGCAAC AATGCCCACG ATGGGGATGG
  241 TGGACTGGGA AGATGGCTCC CATCTCAGGG TGAGGGGCTT CGGCAGCCCC TCATGCTGTA
  301 CATGGCATGT GTATCTCTGC TCTTCTCCAG AAGGCACCAC CACAGCTGCC CACTTCTGGA
  361 AGGTTCTATC TCCTGCTGGT CTGGTCTCCA CAAGCTCAGT GTCCTGAGTT TGGTCCTCGC
  421 CATCCCGCTG CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC CAGCACCTCA
  481 GGGTGGCCTC ATGGTCAGAG ATGGGGTGGT GGGTCACGTG TGTCTTTGGG GGGTCCGCGC
  541 GCTGCAGCGT CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT
  601 CCAGGTAGGC TCTCAGCTGC TCCGCCACAC GGGCCGCCTC CCACTTGCGC TGGGTGATCT
  661 GAGCCGCGGT GTCCGCCGCG GTCCAGGAGC TCAGGTCCTC GTTCAGGGCG ATGTAATCCT
  721 TGCCGTCGTA GGCTAACTGG TTATGCCCGC GGAGGAGGCG CCCGTCCGGC CCCAGGTCGC
  781 AGCCATACAT CGTCTGCCAA GTGTGAGACC CGGCCTCGCT CTGGTTGTAG TAGCCGCGCA
  841 GGTTCCGCAG GCTCTCTCGG TCAGTCTGTG CCTGGGCCTT GTAGATCTGT GTGTTCCGGT
  901 CCCAATACTC CGGCCCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCTCT CTCGGACTCG
  961 CGGCGTCGCT GTCGAACCTC ACGAACTGCG TGTCGTCCAC GTAGCCCACT GCGATGAAGC
 1021 GGGGGCTCCC CGCGGGCCGG GCGGGACATG GGCGGTGTAG AAATACCCTC ATGGGAGTGG
 1081 GGAGCCCGGC CCAAGACTCG ATCAAGGACC CAGGGACCCC CCCAGAAGCA GTCAAGGAAA
1141 GGGAAAAGGG GGT
//
Primer: SV40pro_F_V2 RDB13687_A5Jgc.seq
>03374_13687_A5Jg_SV40pro_F_V2_H06_24.ab1
    1 GAGCCCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
   61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
  841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
  901 GGGGGTTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATCAC
  961 GGCGCTTCTA TGGAAAGGTG GCTTCGGAAT CGTTTTCGGG ACGGCGCCTG ATGATCTCAG
1021 CGCGGGGAAT CTCATGCTGA ATCTCGTCCA CCCGACCTTG TTTCATG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl