Sequencing and PCR primers for primer information.
Human HLA-B*55:02 cDNA from human EBV-transformed B cell line cDNA
| Catalog number | RDB03374 |
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| Resource name | Human HLA-B*55:02 cDNA |
| Alternative name | HLA-B*5502 |
| Clone info. | Human HLA-B*55:02 cDNA from human EBV-transformed B cell line cDNA. |
| Vector backbone | pcDNA3.1(+) (Plasmid) |
| Size of vector backbone | 5.4 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human MHC class I B cDNA |
| Depositor | Akatsuka, Yoshiki | |
| Other clones in our bank | human HLA-B (NCBI Gene 3106) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).) |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB03374 | Human HLA-B*55:02 cDNA | DNA solution |
Electronic file
| Electronic file | Sequence RDB03374z.seq from Depositor |
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Featured content
| Featured content | HLA Clones (en) (English text) |
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| Featured content | HLA Clones (ja) (Japanese text) |
References
| original | Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13687_A5Jgp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB13687_A5Jga.seq |
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>03374_13687_A5Jg_CMV-Forward_A09_03.ab1 1 AGTTAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA 181 ATCTCCTCAG ACACCGAGAT GCGGGTCACG GCACCCCGAA CCCTCCTCCT GCTGCTCTGG 241 GGGGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC 301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA CGTGGACGAC 361 ACGCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GAGAGGAGCC GCGGGCGCCG 421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGAACA CACAGATCTA CAAGGCCCAG 481 GCACAGACTG ACCGAGAGAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA GAGCGAGGCC 541 GGGTCTCACA CTTGGCAGAC GATGTATGGC TGCGACCTGG GGCCGGACGG GCGCCTCCTC 601 CGCGGGCATA ACCAGTTAGC CTACGACGGC AAGGATTACA TCGCCCTGAA CGAGGACCTG 661 AGCTCCTGGA CCGCGGCGGA CACCGCGGCT CAGATCACCC AGCGCAAGTG GGAGGCGGCC 721 CGTGTGGCGG AGCAGCTGAG AGCCTACCTG GAGGGCACGT GCGTGGAGTG GCTCCGCAGA 781 TACCTGGAGA ACGGGAAGGA GACGCTGCAG CGCGCGGACC CCCCAAAGAC ACACGTGACC 841 CACCACCCCA TCTCTGACCA TGAGGCCACC CTGAGGTGCT GGGCCCTGGG CTTCTACCCT 901 GCGGAGATCA CACTGACCTG GCAGCGGGAT GGCGAGGACC AAACTCAGGA CACTGAGCTT 961 GTGGAGACCA GACCAGCAGG AGATAGAACC TTCCAGAAGT GGGCAGCTGT GGTGGTGCCC 1021 TTCTGGGAGA AGAGCAGAGA TACACATGCC CATGTTACAG CATGAGGGGC TGCCGAAGCC 1081 CCTTCACCTG AGATGGGCAG ACATCCTTCC CAGGTTCCAC CCATTCCCCC TCATTCGAAG // |
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| Primer: BGH_rev | RDB13687_A5Jgb.seq |
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>03374_13687_A5Jg_BGHrev_G06_21.ab1 1 GTTCGGGACT CTAGACTCGA GCGGCCGCAT CTCAGTCCCT CACAAGACAG CTGTCTCAGG 61 CTTTTCAAGC TGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCGCTGGAC GCAGCCTGAG 121 AGTAGCTCCC TCCTTTTCCA CCTGAGCTCT TCCTCCTACA CATCACAGTA GCGACCACAG 181 CTCCGATGAC CACAACTGCT AGGACAGCCA GGCCAGCAAC AATGCCCACG ATGGGGATGG 241 TGGACTGGGA AGATGGCTCC CATCTCAGGG TGAGGGGCTT CGGCAGCCCC TCATGCTGTA 301 CATGGCATGT GTATCTCTGC TCTTCTCCAG AAGGCACCAC CACAGCTGCC CACTTCTGGA 361 AGGTTCTATC TCCTGCTGGT CTGGTCTCCA CAAGCTCAGT GTCCTGAGTT TGGTCCTCGC 421 CATCCCGCTG CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC CAGCACCTCA 481 GGGTGGCCTC ATGGTCAGAG ATGGGGTGGT GGGTCACGTG TGTCTTTGGG GGGTCCGCGC 541 GCTGCAGCGT CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT 601 CCAGGTAGGC TCTCAGCTGC TCCGCCACAC GGGCCGCCTC CCACTTGCGC TGGGTGATCT 661 GAGCCGCGGT GTCCGCCGCG GTCCAGGAGC TCAGGTCCTC GTTCAGGGCG ATGTAATCCT 721 TGCCGTCGTA GGCTAACTGG TTATGCCCGC GGAGGAGGCG CCCGTCCGGC CCCAGGTCGC 781 AGCCATACAT CGTCTGCCAA GTGTGAGACC CGGCCTCGCT CTGGTTGTAG TAGCCGCGCA 841 GGTTCCGCAG GCTCTCTCGG TCAGTCTGTG CCTGGGCCTT GTAGATCTGT GTGTTCCGGT 901 CCCAATACTC CGGCCCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCTCT CTCGGACTCG 961 CGGCGTCGCT GTCGAACCTC ACGAACTGCG TGTCGTCCAC GTAGCCCACT GCGATGAAGC 1021 GGGGGCTCCC CGCGGGCCGG GCGGGACATG GGCGGTGTAG AAATACCCTC ATGGGAGTGG 1081 GGAGCCCGGC CCAAGACTCG ATCAAGGACC CAGGGACCCC CCCAGAAGCA GTCAAGGAAA 1141 GGGAAAAGGG GGT // |
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| Primer: SV40pro_F_V2 | RDB13687_A5Jgc.seq |
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>03374_13687_A5Jg_SV40pro_F_V2_H06_24.ab1 1 GAGCCCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 901 GGGGGTTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATCAC 961 GGCGCTTCTA TGGAAAGGTG GCTTCGGAAT CGTTTTCGGG ACGGCGCCTG ATGATCTCAG 1021 CGCGGGGAAT CTCATGCTGA ATCTCGTCCA CCCGACCTTG TTTCATG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. |
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2018.12.11
GNP_filter3_RDBDEP_html_181012.pl