RDB03373 - Human HLA-B*54:01 cDNA

Resource data sheet
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Human HLA-B*54:01 cDNA

Human HLA-B*54:01 cDNA from human EBV-transformed B cell line cDNA


Catalog number	RDB03373
Resource name	Human HLA-B*54:01 cDNA
Alternative name	HLA-B*5401
Clone info.	Human HLA-B*54:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human MHC class I B cDNA
Depositor	Akatsuka, Yoshiki \|
Other clones in our bank	human HLA-B (NCBI Gene 3106) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB03373	Human HLA-B*54:01 cDNA	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB03373z.seq from Depositor

Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB03373_A3G8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp	RDB03373_A3G8a.seq
>03373_03373_A3G8_T7_20bp_E06_15.ab1 1 CGTACAGCGT TACTTAAGCT TCACCCGGAC TCAGAATCTC CTCAGACACC GAGATGCGGG 61 TCACGGCACC CCGAACCCTC CTCCTGCTGC TCTGGGGGGC CCTGGCCCTG ACCGAGACCT 121 GGGCCGGCTC CCACTCCATG AGGTATTTCT ACACCGCCAT GTCCCGGCCC GGCCGCGGGG 181 AGCCCCGCTT CATCGCAGTG GGCTACGTGG ACGACACGCA GTTCGTGCGG TTCGACAGCG 241 ACGCCGCGAG TCCGAGAGGG GAGCCGCGGG CGCCGTGGGT GGAGCAGGAG GGGCCGGAGT 301 ATTGGGACCG GAACACACAG ATCTACAAGG CCCAGGCACA GACTGACCGA GAGAGCCTGC 361 GGAACCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACTTGG CAGACGATGT 421 ATGGCTGCGA CCTGGGGCCG GACGGGCGCC TCCTCCGCGG GCATAACCAG TTAGCCTACG 481 ACGGCAAGGA TTACATCGCC CTGAACGAGG ACCTGAGCTC CTGGACCGCG GCGGACACCG 541 CGGCTCAGAT CACCCAGCGC AAGTGGGAGG CGGCCCGTGT GGCGGAGCAG CTGAGAGCCT 601 ACCTGGAGGG CACGTGCGTG GAGTGGCTCC GCAGATACCT GGAGAACGGG AAGGAGACGC 661 TGCAGCGCGC GGACCCCCCA AAGACACACG TGACCCACCA CCCCATCTCT GACCATGAGG 721 CCACCCTGAG GTGCTGGGCC CTGGGCTTCT ACCCTGCGGA GATCACACTG ACCTGGCAGC 781 GGGATGGCGA GGACCAAACT CAGGACACTG AGCTTGTGGA GACCAGACCA GCAGGAGATA 841 GAACCTTCCA GAAGTGGGCA GCTGTGGTGG TGCCTTCTGG AGAAGAGCAG AGATACACAT 901 GCCATGTACA GCATGAGGGG CTGCCGAAGC CCCTCACCCT GAGATGGGAG CCATCTTCCC 961 AGTCCACCAT CCCCATCGTG GGCATTGTTG CTGGGCCTGG CTGTCCTAGC AGTTGTGGTC 1021 ATCGGGAGCT GTGGTCGCTA CTGTGATGTG TAGGAGAAGA AGCTCAAGTG GAAAAGGAAG 1081 GGAGCTACTC TCAAGGCTGC GTCCAGCGAA CAGTGGCCAA GGCTCTGAAT GTTGTCTCCT 1141 CCACAAGCCT GCAAAAGGCT GAAAAACAGC TGACTGCTGA AGGAACTGGA AGAAATGTAC 1201 CCG //
Primer: BGH_rev	RDB03373_A3G8b.seq
>03373_03373_A3G8_BGHrev_F06_18.ab1 1 CTTACGGGGA CTCTAGACTC GAGCGGCCGC ATCTCAGTCC CTCACAAGAC AGCTGTCTCA 61 GGCTTTTCAA GCTGTGAGAG ACACATCAGA GCCCTGGGCA CTGTCGCTGG ACGCAGCCTG 121 AGAGTAGCTC CCTCCTTTTC CACCTGAGCT CTTCCTCCTA CACATCACAG TAGCGACCAC 181 AGCTCCGATG ACCACAACTG CTAGGACAGC CAGGCCAGCA ACAATGCCCA CGATGGGGAT 241 GGTGGACTGG GAAGATGGCT CCCATCTCAG GGTGAGGGGC TTCGGCAGCC CCTCATGCTG 301 TACATGGCAT GTGTATCTCT GCTCTTCTCC AGAAGGCACC ACCACAGCTG CCCACTTCTG 361 GAAGGTTCTA TCTCCTGCTG GTCTGGTCTC CACAAGCTCA GTGTCCTGAG TTTGGTCCTC 421 GCCATCCCGC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG AAGCCCAGGG CCCAGCACCT 481 CAGGGTGGCC TCATGGTCAG AGATGGGGTG GTGGGTCACG TGTGTCTTTG GGGGGTCCGC 541 GCGCTGCAGC GTCTCCTTCC CGTTCTCCAG GTATCTGCGG AGCCACTCCA CGCACGTGCC 601 CTCCAGGTAG GCTCTCAGCT GCTCCGCCAC ACGGGCCGCC TCCCACTTGC GCTGGGTGAT 661 CTGAGCCGCG GTGTCCGCCG CGGTCCAGGA GCTCAGGTCC TCGTTCAGGG CGATGTAATC 721 CTTGCCGTCG TAGGCTAACT GGTTATGCCC GCGGAGGAGG CGCCCGTCCG GCCCCAGGTC 781 GCAGCCATAC ATCGTCTGCC AAGTGTGAGA CCCGGCCTCG CTCTGGTTGT AGTAGCCGCG 841 CAGGTTCCGC AGGCTCTCTC GGTCAGTCTG TGCCTGGGCC TTGTAGATCT GTGTGTTCCG 901 GTCCCAATAC TCCGGCCCCT CCTGCTCCAC CCACGGCGCC CGCGGCTCCC CTCTCGGACT 961 CGCGGCGTCG CTGTCGAACC GCACGAACTG CGTGTCGTCC ACGTAGCCCA CTGCGATGAA 1021 GCGGGGCTCC CCGCGGGCCG GGCCGGGACA TGGCGGTGTA GAAATACCCT CATGGAGTGG 1081 GAGCGACCAG TCTCGGGTCA GGACAAGGAC TCCCTCAGAG CCAGCAAGGA GGAGGATTCG 1141 GGGGGTGTCG TGAACCCGGC CAAT //
Primer: SV40pro_F	RDB03373_A3G8c.seq
>03373_03373_A3G8_SV40pro_F_B03_06.ab1 1 CTTGTCTGGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT 121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTTCTATCGC 901 CTTTCTTGAC GAGTTTCTTT CTGAGCGGGA CTCTGGGGGT TTCGAAATGA CCCGACCAGC 961 GACGCCCATC CTTGCCATCA CGAGATTTCC GAATTCAACG ACGCGTTCTA TGGAATGTGG 1021 CCTTCGGGAT CGTTTCTGGA ACGCGCTGTA TGATCTCAGC GCGGGCAATC TCATGCTGGG 1081 A //

Please visit Sequencing and PCR primers for primer information.

References

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl