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Human HLA-B*54:01 cDNA

Human HLA-B*54:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03373
Resource name Human HLA-B*54:01 cDNA
Alternative name HLA-B*5401
Clone info. Human HLA-B*54:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB03373 Human HLA-B*54:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB03373z.seq

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15309_A7H1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB15309_A7H1a.seq check
          >03373_15309_A7H1_CMV-Forward_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTGN NGCNNTGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTCAC CCGGACTCAG
            181 AATCTCCTCA GACACCGAGA TGCGGGTCAC GGCACCCCGA ACCCTCCTCC TGCTGCTCTG
            241 GGGGGCCCTG GCCCTGACCG AGACCTGGGC CGGCTCCCAC TCCATGAGGT ATTTCTACAC
            301 CGCCATGTCC CGGCCCGGCC GCGGGGAGCC CCGCTTCATC GCAGTGGGCT ACGTGGACGA
            361 CACGCAGTTC GTGCGGTTCG ACAGCGACGC CGCGAGTCCG AGAGGGGAGC CGCGGGCGCC
            421 GTGGGTGGAG CAGGAGGGGC CGGAGTATTG GGACCGGAAC ACACAGATCT ACAAGGCCCA
            481 GGCACAGACT GACCGAGAGA GCCTGCGGAA CCTGCGCGGC TACTACAACC AGAGCGAGGC
            541 CGGGTCTCAC ACTTGGCAGA CGATGTATGG CTGCGACCTG GGGCCGGACG GGCGCCTCCT
            601 CCGCGGGCAT AACCAGTTAG CCTACGACGG CAAGGATTAC ATCGCCCTGA ACGAGGACCT
            661 GAGCTCCTGG ACCGCGGCGG ANACCGCGGC TCAGATCACC CAGCGCAAGT GGGAGGCGGC
            721 CCGTGTGGCG GANCAGCTGA NAGCCTACCT GGNNGGCACG TGCGTGGAGT GNNTCCGCAG
            781 ATACCTGGAG AACGGGAAGG AGACGCTGCA GCGCGCGGAC CCCCCAAAGA CACACGNGAC
            841 CCACCACCCC ATCTCTGACC ATGNNGNCAC CCTGANGNGC TGGGCCCNNG GNNNCTACCC
            901 NGCNGANATC ACANNNNNCN NGNNANCGGG NTGGNGANGN NCNAACTCNN NANNNNNNNN
          961 TTGNGGANAN NNNANCANCN NNNNNNNNN
          //
          Primer: BGH_rev2
          Region: bGHpA,insert 3'
          Sequence file: RDB15309_A7H1b.seq check
          >03373_15309_A7H1_BGH_rev2_G02_20_ABI24.ab1
              1 NNNNNNNNNG NNNNNNGGGG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC
            121 TCACAAGACA GCTGTCTCAG GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC
            181 TGTCGCTGGA CGCAGCCTGA GAGTAGCTCC CTCCTTTTCC ACCTGAGCTC TTCCTCCTAC
            241 ACATCACAGT AGCGACCACA GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA
            301 CAATGCCCAC GATGGGGATG GTGGACTGGG AAGATGGCTC CCATCTCAGG GTGAGGGGCT
            361 TCGGCAGCCC CTCATGCTGT ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA
            421 CCACAGCTGC CCACTTCTGG AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG
            481 TGTCCTGAGT TTGGTCCTCG CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA
            541 AGCCCAGGGC CCAGCACCTC AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACGT
            601 GTGTCTTTGG GGGGTCCGCG CGCTGCAGCG TCTCCTTCCC GTTCTCCAGG TATCTGCGGA
            661 GCCACTCCAC GCACGTGCCC TCCAGGTAGG CTCTCAGCTG CTCCGCCACA CGGGCCGCCT
            721 CCCACTTGCG CTGGGTGATC TGAGCCGCGG TGTCCGCCGC GGTCCANGAN CTCANGTCCT
            781 CGTTCANGGC GATGTAATCC TTGCCGTCGT NNNTAACTGG TTATGCCCGC GGANGANGCG
            841 CCCGTCCNGN CCCAGGNCGC ANCCNTACAT CGTCTGCCAA GNGTGAGANC NNGCCTCGNN
            901 CNNGTNGNAG TAGCNGCGCN NNNNCGCNNN CTNTNNNGNN CAGTCNGTGN CNGGNNNNNN
          961 NANNNNNNNN NN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB15309_A7H1c.seq check
          >03373_15309_B0FtS_SV40pro_ori_F_G03_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG CCTCNGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
             61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
            121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
            181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
            241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
            301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
            361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
            421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
            481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
            541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
            601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
            661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGANGNT CTCGTCGTGA CCCATGGCGA
            721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACNGTGG
            781 CCGGCTNGGT GTGGCGGANC GCTATCANGA NATANCGTTG GCTACCNGNG ATATTGCTGA
            841 AGANCTNNNN NGCGAANGGG CNNNNNGCTN NCNCNNNNNT NACGGNATCG CCNCNNCNNA
            901 NTCNCNNCGN ATCNNCNTNC NNNCNNCNNN NNNGNNNANN NNNTNNNNNN NNGNNNNNNN
          961 GGGNNNNNNN AANNNNNCNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl