Resource data sheet
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Human HLA-B*54:01 cDNA

Human HLA-B*54:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03373
Resource name Human HLA-B*54:01 cDNA
Alternative name HLA-B*5401
Clone info. Human HLA-B*54:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03373 Human HLA-B*54:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03373z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB03373_A3G8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB03373_A3G8a.seq
>03373_03373_A3G8_T7_20bp_E06_15.ab1
    1 CGTACAGCGT TACTTAAGCT TCACCCGGAC TCAGAATCTC CTCAGACACC GAGATGCGGG
   61 TCACGGCACC CCGAACCCTC CTCCTGCTGC TCTGGGGGGC CCTGGCCCTG ACCGAGACCT
  121 GGGCCGGCTC CCACTCCATG AGGTATTTCT ACACCGCCAT GTCCCGGCCC GGCCGCGGGG
  181 AGCCCCGCTT CATCGCAGTG GGCTACGTGG ACGACACGCA GTTCGTGCGG TTCGACAGCG
  241 ACGCCGCGAG TCCGAGAGGG GAGCCGCGGG CGCCGTGGGT GGAGCAGGAG GGGCCGGAGT
  301 ATTGGGACCG GAACACACAG ATCTACAAGG CCCAGGCACA GACTGACCGA GAGAGCCTGC
  361 GGAACCTGCG CGGCTACTAC AACCAGAGCG AGGCCGGGTC TCACACTTGG CAGACGATGT
  421 ATGGCTGCGA CCTGGGGCCG GACGGGCGCC TCCTCCGCGG GCATAACCAG TTAGCCTACG
  481 ACGGCAAGGA TTACATCGCC CTGAACGAGG ACCTGAGCTC CTGGACCGCG GCGGACACCG
  541 CGGCTCAGAT CACCCAGCGC AAGTGGGAGG CGGCCCGTGT GGCGGAGCAG CTGAGAGCCT
  601 ACCTGGAGGG CACGTGCGTG GAGTGGCTCC GCAGATACCT GGAGAACGGG AAGGAGACGC
  661 TGCAGCGCGC GGACCCCCCA AAGACACACG TGACCCACCA CCCCATCTCT GACCATGAGG
  721 CCACCCTGAG GTGCTGGGCC CTGGGCTTCT ACCCTGCGGA GATCACACTG ACCTGGCAGC
  781 GGGATGGCGA GGACCAAACT CAGGACACTG AGCTTGTGGA GACCAGACCA GCAGGAGATA
  841 GAACCTTCCA GAAGTGGGCA GCTGTGGTGG TGCCTTCTGG AGAAGAGCAG AGATACACAT
  901 GCCATGTACA GCATGAGGGG CTGCCGAAGC CCCTCACCCT GAGATGGGAG CCATCTTCCC
  961 AGTCCACCAT CCCCATCGTG GGCATTGTTG CTGGGCCTGG CTGTCCTAGC AGTTGTGGTC
 1021 ATCGGGAGCT GTGGTCGCTA CTGTGATGTG TAGGAGAAGA AGCTCAAGTG GAAAAGGAAG
 1081 GGAGCTACTC TCAAGGCTGC GTCCAGCGAA CAGTGGCCAA GGCTCTGAAT GTTGTCTCCT
 1141 CCACAAGCCT GCAAAAGGCT GAAAAACAGC TGACTGCTGA AGGAACTGGA AGAAATGTAC
1201 CCG
//
Primer: BGH_rev RDB03373_A3G8b.seq
>03373_03373_A3G8_BGHrev_F06_18.ab1
    1 CTTACGGGGA CTCTAGACTC GAGCGGCCGC ATCTCAGTCC CTCACAAGAC AGCTGTCTCA
   61 GGCTTTTCAA GCTGTGAGAG ACACATCAGA GCCCTGGGCA CTGTCGCTGG ACGCAGCCTG
  121 AGAGTAGCTC CCTCCTTTTC CACCTGAGCT CTTCCTCCTA CACATCACAG TAGCGACCAC
  181 AGCTCCGATG ACCACAACTG CTAGGACAGC CAGGCCAGCA ACAATGCCCA CGATGGGGAT
  241 GGTGGACTGG GAAGATGGCT CCCATCTCAG GGTGAGGGGC TTCGGCAGCC CCTCATGCTG
  301 TACATGGCAT GTGTATCTCT GCTCTTCTCC AGAAGGCACC ACCACAGCTG CCCACTTCTG
  361 GAAGGTTCTA TCTCCTGCTG GTCTGGTCTC CACAAGCTCA GTGTCCTGAG TTTGGTCCTC
  421 GCCATCCCGC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG AAGCCCAGGG CCCAGCACCT
  481 CAGGGTGGCC TCATGGTCAG AGATGGGGTG GTGGGTCACG TGTGTCTTTG GGGGGTCCGC
  541 GCGCTGCAGC GTCTCCTTCC CGTTCTCCAG GTATCTGCGG AGCCACTCCA CGCACGTGCC
  601 CTCCAGGTAG GCTCTCAGCT GCTCCGCCAC ACGGGCCGCC TCCCACTTGC GCTGGGTGAT
  661 CTGAGCCGCG GTGTCCGCCG CGGTCCAGGA GCTCAGGTCC TCGTTCAGGG CGATGTAATC
  721 CTTGCCGTCG TAGGCTAACT GGTTATGCCC GCGGAGGAGG CGCCCGTCCG GCCCCAGGTC
  781 GCAGCCATAC ATCGTCTGCC AAGTGTGAGA CCCGGCCTCG CTCTGGTTGT AGTAGCCGCG
  841 CAGGTTCCGC AGGCTCTCTC GGTCAGTCTG TGCCTGGGCC TTGTAGATCT GTGTGTTCCG
  901 GTCCCAATAC TCCGGCCCCT CCTGCTCCAC CCACGGCGCC CGCGGCTCCC CTCTCGGACT
  961 CGCGGCGTCG CTGTCGAACC GCACGAACTG CGTGTCGTCC ACGTAGCCCA CTGCGATGAA
 1021 GCGGGGCTCC CCGCGGGCCG GGCCGGGACA TGGCGGTGTA GAAATACCCT CATGGAGTGG
 1081 GAGCGACCAG TCTCGGGTCA GGACAAGGAC TCCCTCAGAG CCAGCAAGGA GGAGGATTCG
1141 GGGGGTGTCG TGAACCCGGC CAAT
//
Primer: SV40pro_F RDB03373_A3G8c.seq
>03373_03373_A3G8_SV40pro_F_B03_06.ab1
    1 CTTGTCTGGG ACATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTTCTATCGC
  901 CTTTCTTGAC GAGTTTCTTT CTGAGCGGGA CTCTGGGGGT TTCGAAATGA CCCGACCAGC
  961 GACGCCCATC CTTGCCATCA CGAGATTTCC GAATTCAACG ACGCGTTCTA TGGAATGTGG
 1021 CCTTCGGGAT CGTTTCTGGA ACGCGCTGTA TGATCTCAGC GCGGGCAATC TCATGCTGGG
1081 A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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