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Human HLA-B*54:01 cDNA

Human HLA-B*54:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03373
Resource name Human HLA-B*54:01 cDNA
Alternative name HLA-B*5401
Clone info. Human HLA-B*54:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03373 Human HLA-B*54:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB03373z.seq

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15309_A7H1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB15309_A7H1a.seq check
>03373_15309_A7H1_CMV-Forward_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTGN NGCNNTGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTCAC CCGGACTCAG
  181 AATCTCCTCA GACACCGAGA TGCGGGTCAC GGCACCCCGA ACCCTCCTCC TGCTGCTCTG
  241 GGGGGCCCTG GCCCTGACCG AGACCTGGGC CGGCTCCCAC TCCATGAGGT ATTTCTACAC
  301 CGCCATGTCC CGGCCCGGCC GCGGGGAGCC CCGCTTCATC GCAGTGGGCT ACGTGGACGA
  361 CACGCAGTTC GTGCGGTTCG ACAGCGACGC CGCGAGTCCG AGAGGGGAGC CGCGGGCGCC
  421 GTGGGTGGAG CAGGAGGGGC CGGAGTATTG GGACCGGAAC ACACAGATCT ACAAGGCCCA
  481 GGCACAGACT GACCGAGAGA GCCTGCGGAA CCTGCGCGGC TACTACAACC AGAGCGAGGC
  541 CGGGTCTCAC ACTTGGCAGA CGATGTATGG CTGCGACCTG GGGCCGGACG GGCGCCTCCT
  601 CCGCGGGCAT AACCAGTTAG CCTACGACGG CAAGGATTAC ATCGCCCTGA ACGAGGACCT
  661 GAGCTCCTGG ACCGCGGCGG ANACCGCGGC TCAGATCACC CAGCGCAAGT GGGAGGCGGC
  721 CCGTGTGGCG GANCAGCTGA NAGCCTACCT GGNNGGCACG TGCGTGGAGT GNNTCCGCAG
  781 ATACCTGGAG AACGGGAAGG AGACGCTGCA GCGCGCGGAC CCCCCAAAGA CACACGNGAC
  841 CCACCACCCC ATCTCTGACC ATGNNGNCAC CCTGANGNGC TGGGCCCNNG GNNNCTACCC
  901 NGCNGANATC ACANNNNNCN NGNNANCGGG NTGGNGANGN NCNAACTCNN NANNNNNNNN
961 TTGNGGANAN NNNANCANCN NNNNNNNNN
//
Primer: BGH_rev2
Region: bGHpA,insert 3'
Sequence file: RDB15309_A7H1b.seq check
>03373_15309_A7H1_BGH_rev2_G02_20_ABI24.ab1
    1 NNNNNNNNNG NNNNNNGGGG GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC
  121 TCACAAGACA GCTGTCTCAG GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC
  181 TGTCGCTGGA CGCAGCCTGA GAGTAGCTCC CTCCTTTTCC ACCTGAGCTC TTCCTCCTAC
  241 ACATCACAGT AGCGACCACA GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA
  301 CAATGCCCAC GATGGGGATG GTGGACTGGG AAGATGGCTC CCATCTCAGG GTGAGGGGCT
  361 TCGGCAGCCC CTCATGCTGT ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA
  421 CCACAGCTGC CCACTTCTGG AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG
  481 TGTCCTGAGT TTGGTCCTCG CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA
  541 AGCCCAGGGC CCAGCACCTC AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACGT
  601 GTGTCTTTGG GGGGTCCGCG CGCTGCAGCG TCTCCTTCCC GTTCTCCAGG TATCTGCGGA
  661 GCCACTCCAC GCACGTGCCC TCCAGGTAGG CTCTCAGCTG CTCCGCCACA CGGGCCGCCT
  721 CCCACTTGCG CTGGGTGATC TGAGCCGCGG TGTCCGCCGC GGTCCANGAN CTCANGTCCT
  781 CGTTCANGGC GATGTAATCC TTGCCGTCGT NNNTAACTGG TTATGCCCGC GGANGANGCG
  841 CCCGTCCNGN CCCAGGNCGC ANCCNTACAT CGTCTGCCAA GNGTGAGANC NNGCCTCGNN
  901 CNNGTNGNAG TAGCNGCGCN NNNNCGCNNN CTNTNNNGNN CAGTCNGTGN CNGGNNNNNN
961 NANNNNNNNN NN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB15309_A7H1c.seq check
>03373_15309_B0FtS_SV40pro_ori_F_G03_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG CCTCNGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
  181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
  241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
  301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
  361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
  421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
  481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
  541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
  601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
  661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGANGNT CTCGTCGTGA CCCATGGCGA
  721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACNGTGG
  781 CCGGCTNGGT GTGGCGGANC GCTATCANGA NATANCGTTG GCTACCNGNG ATATTGCTGA
  841 AGANCTNNNN NGCGAANGGG CNNNNNGCTN NCNCNNNNNT NACGGNATCG CCNCNNCNNA
  901 NTCNCNNCGN ATCNNCNTNC NNNCNNCNNN NNNGNNNANN NNNTNNNNNN NNGNNNNNNN
961 GGGNNNNNNN AANNNNNCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl