Resource data sheet
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Human HLA-B*48:01 cDNA

Human HLA-B*48:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03372
Resource name Human HLA-B*48:01 cDNA
Alternative name HLA-B*4801
Clone info. Human HLA-B*48:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03372 Human HLA-B*48:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03372z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13719_A5Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13719_A5Lla.seq
>03372_13719_A5Ll_T7_20bp_F04_16.ab1
    1 GATTGGAGGG CGGTTTAAAC TTTAAGGCTT TCACCCGGAC TCAGAAATCT CCTCAGACGC
   61 CGAGATGCTG GTCATGGCGC CCCGAACCGT CCTCCTGCTG CTCTCGGCGG CCCTGGCCCT
  121 GACCGAGACC TGGGCCGGCT CCCACTCCAT GAGGTATTTC TACACCTCCG TGTCCCGGCC
  181 CGGCCGCGGG GAGCCCCGCT TCATCTCAGT GGGCTACGTG GACGACACCC AGTTCGTGAG
  241 GTTCGACAGC GACGCCGCGA GTCCGAGAGA GGAGCCGCGG GCGCCGTGGA TAGAGCAGGA
  301 GGGGCCGGAG TATTGGGACC GGGAGACACA GATCTCCAAG ACCAACACAC AGACTTACCG
  361 AGAGAGCCTG CGGAACCTGC GCGGCTACTA CAACCAGAGC GAGGCCGGGT CTCACACCCT
  421 CCAGAGCATG TACGGCTGCG ACGTGGGGCC GGACGGGCGC CTCCTCCGCG GGCATAACCA
  481 GTACGCCTAC GACGGCAAGG ATTACATCGC CCTGAACGAG GACCTGCGCT CCTGGACCGC
  541 CGCGGACACG GCGGCTCAGA TCTCCCAGCG CAAGTTGGAG GCGGCCCGTG TGGCGGAGCA
  601 GCTGAGAGCC TACCTGGAGG GCGAGTGCGT GGAGTGGCTC CGCAGATACC TGGAGAACGG
  661 GAAGGACAAG CTGGAGCGCG CTGACCCCCC AAAGACACAC GTGACCCACC ACCCCATCTC
  721 TGACCATGAG GCCACCCTGA GGTGCTGGGC CCTGGGTTTC TACCCTGCGG AGATCACACT
  781 GACCTGGCAG CGGGATGGCG AGGACCAAAC TCAGGACACT GAGCTTGTGG AGACCAGACC
  841 AGCAGGAGAT AGAACCTTCC AGAAGTGGGA CAGGCTGTGG TGGTGCCTTT CTGGAGAAGA
  901 GGCAGGAGAT ACACATGCCA TGTACAGCAT GAGGGGGCTG CCGAAGCCCC TCACCCTGAG
  961 ATGGGAGCGG TCTTTCCCAG TCCACCGGTC CCCATCGTGG GGCATTGTTG CTGGGCCTGG
 1021 CTGTCCTAGC AGTTGGTGGT CCATCGGAAG CTGTGGGTCG CCTTGCTGTG ATTGTGTAAG
 1081 AAGGAAGAAG TTTCAGGGTG AAAAGAAAGG GAGCTACTTC TCAAGGCTGC ATGCAACGGA
1141 ACAGTGTGCC AAGGGGCTCT CTGGAATGTG TGTCTCTCTC ACACATCCTT GGTGAAAC
//
Primer: BGH_rev RDB13719_A5Llb.seq
>03372_13719_A5Ll_T7_BGHrev_G04_19.ab1
    1 GATCGGGTCC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC TCACAAGACA GCTGTCTCAG
   61 GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC TGTCGCTGCA CGCAGCCTGA
  121 GAGTAGCTCC CTCCTTTTCC ACCTGAACTC TTCCTCCTAC ACATCACAGC AGCGACCACA
  181 GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA CAATGCCCAC GATGGGGACG
  241 GTGGACTGGG AAGACGGCTC CCATCTCAGG GTGAGGGGCT TCGGCAGCCC CTCATGCTGT
  301 ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA CCACAGCTGT CCACTTCTGG
  361 AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG TGTCCTGAGT TTGGTCCTCG
  421 CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA AACCCAGGGC CCAGCACCTC
  481 AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACGT GTGTCTTTGG GGGGTCAGCG
  541 CGCTCCAGCT TGTCCTTCCC GTTCTCCAGG TATCTGCGGA GCCACTCCAC GCACTCGCCC
  601 TCCAGGTAGG CTCTCAGCTG CTCCGCCACA CGGGCCGCCT CCAACTTGCG CTGGGAGATC
  661 TGAGCCGCCG TGTCCGCGGC GGTCCAGGAG CGCAGGTCCT CGTTCAGGGC GATGTAATCC
  721 TTGCCGTCGT AGGCGTACTG GTTATGCCCG CGGAGGAGGC GCCCGTCCGG CCCCACGTCG
  781 CAGCCGTACA TGCTCTGGAG GGTGTGAGAC CCGGCCTCGC TCTGGTTGTA GTAGCCGCGC
  841 AGGTTCCGCA GGCTCTCTCG GTAAGTCTGT GTGTTGGTCT TGGAGATCTG TGTCTCCCGG
  901 TCCCAATACT CCGGCCCCTC CTGCTCTATC CACGGCGCCC GCGGCTCCTC TCTCGGACTC
  961 GCGGGCGTCG CTGTCGAACC TCACGAACTG GGTGTCGTCC ACGTAGCCCC ACTGAGATGA
 1021 AGCGGGGCTC CCCCGCGGCC CGGGCCGGGA CCACGGAGTG TAGAAATACC CTCATGGGAG
 1081 TGGGGAGCCG GCCCAGGTCC TCGGTCAGGG ACCAAGGTGC GAGAGCAGCA GAGGACGATC
1141 GGGGGCGCCC ATGAACCAAG CCAATCTCTC TCGGGCGT
//
Primer: SV40pro_F RDB13719_A5Llc.seq
>03372_13719_A5Ll_SV40pro_F_A08_02.ab1
    1 CATTCGGTCC CGTGGCTTTC AGAGGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
  121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
  661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  901 TCGCCTTCTT GACGAGTTTC TTCTGAGCGG GACTCTGGGG GTTTCGAAAT GACCGACCAA
  961 GCGAACGCCC ACCTGCCATC ACGAGATTCC GATTTCACGG CCGCTTTCTA TGGAAGGATG
1021 GCTCCGAATC GTTTTCGGAC GCTGGCTGTA TGATCTCAGC GTCTGGAAAT TC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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