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Human HLA-B*39:01:01 cDNA

Human HLA-B*39:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03371
Resource name Human HLA-B*39:01:01 cDNA
Alternative name HLA-B*39011
Clone info. Human HLA-B*39:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03371 Human HLA-B*39:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB03371z.seq
Electronic file Map RDB03371.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18240_B0Gdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB18240_B0Gda.seq check
>03371_18240_B0Gd_1_CMV_Forward_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTGN NGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTCA CCCGGACTCA
  181 GAATCTCCTC AGACGCCGAG ATGCTGGTCA TGGCGCCCCG AACCGTCCTC CTGCTGCTCT
  241 CGGCGGCCCT GGCCCTGACC GAGACCTGGG CCGGCTCCCA CTCCATGAGG TATTTCTACA
  301 CCTCCGTGTC CCGGCCCGGC CGCGGGGAGC CCCGCTTCAT CTCAGTGGGC TACGTGGACG
  361 ACACGCAGTT CGTGAGGTTC GACAGCGACG CCGCGAGTCC GAGAGAGGAG CCGCGGGCGC
  421 CGTGGATAGA GCAGGAGGGG CCGGAATATT GGGACCGGAA CACACAGATC TGCAAGACCA
  481 ACACACAGAC TGACCGAGAG AGCCTGCGGA ACCTGCGCGG CTACTACAAC CAGAGCGAGG
  541 CCGGGTCTCA CACCCTCCAG AGGATGTACG GCTGCGACGT GGGGCCGGAC GGGCGCCTCC
  601 TCCGCGGGCA TAACCAGTTC GCCTACGACG GCAAGGATTA CATCGCCCTG AACGAGGACC
  661 TGAGCTCCTG GACCGCGGCG GACACCGCGG CTCAGATCAC CCAGCGCAAG TGGGANGCGG
  721 CCCGTGTGGC GGANCAGCTG AGAACCTACC TGGAGGGCAC GTGCGTGGAG TGGCTCCGCA
  781 GATACCTGGA GAACGGGAAG GANANGCTGC AGCGCGCGGA CCCCCCNAAG ANACNTGTGA
  841 NCCACCACCC NNTCTCTGAN NNTGANGCCA CCCTGANGNG CTGGGCCCNG GGNTNCTACC
  901 CTGCGGANAT CACANNGANC NNNNNNCGGG NNNGCGAGGN NCAAACNNNN NNNNCNANCT
  961 TGNNGNANAN CANNNCNGCN NNNANANNNN NTTNCANANN NNGNCANCNN NNGNNGNNNN
1021 NNNNNN
//
Primer: BGH_rev2
Region: bGHpA, insert 3'
Sequence file: RDB18240_B0Gdb.seq check
>03371_18240_B0Gd_1_bGH_rev3_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGN NGNNGGNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC ATCTCAGTCC
  121 CTCACAAGAC AGCTGTCTCA GGCTTTTCAA GCTGTGAGAG ACACATCAGA GCCCTGGGCA
  181 CTGTCGCTGG ACGCAGCCTG AGAGTAGCTC CCTCCTTTTC CACCTGAACT CTTCCTCCTA
  241 CACATCACAG CAGCGACCAC AGCTCCGATG ACCACAACTG CTAGGACAGC CAGGCCAGCA
  301 ACAATGCCCA CGATGGGGAC GGTGGACTGG GAAGATGGCT CCCATCTCAG GGTGAGGGGC
  361 TTCGGCAGCC CCTCATGCTG TACATGGCAT GTGTATCTCT GCTCTTCTCC AGAAGGCACC
  421 ACCACAGCTG CCCACTTCTG GAAGGTTCTG TCTCCTGCTG GTCTGGTCTC CACAAGCTCG
  481 GTGTCCTGAG TTTGGTCCTC GCCATCCCGC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG
  541 AAGCCCAGGG CCCAGCACCT CAGGGTGGCC TCATGGTCAG AGATGGGGTG GTGGGTCACA
  601 TGTGTCTTTG GGGGGTCCGC GCGCTGCAGC GTCTCCTTCC CGTTCTCCAG GTATCTGCGG
  661 AGCCACTCCA CGCACGTGCC CTCCAGGTAG GTTCTCAGCT GCTCCGCCAC ACGGGCCGCC
  721 TCCCACTTGC GCTGGGTGAT CTGAGCCGCG GTGTCCGCCG CGGTCCAGGA GCTCANGTCC
  781 TCGTTCANGG CGATGTAATC CTTGCCGTCG TANGCGAACT GGTTATGCCC GCGGANGANG
  841 CGCCCGTCCG GCCCCACGTC GCANCCGTAC ATCCTCTGGA NGGTGTGAGA NCCGGCCTCG
  901 CTCTGGTTGN AGTANCCGCG CANGTTCCGC ANNNTCTCTC GGNCAGTCTG TGTGNTGNNC
  961 TTGCANATCT GTGTGTTCCG GNCCCAANAT TNCNNNNCCN NNCNGNNNNN TCCNNNNNGC
1021 CNNNNGNNNN NNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB18240_B0Gdc.seq check
>03371_18240_B0Gd_1_SV40pro_ori_F_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNNGNCNCC TCTGCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
  121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
  181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
  241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
  301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
  361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
  421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
  481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
  541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
  601 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT
  661 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG
  721 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGANTCATC GACTGTGGCC
  781 GGCTGGGTGT GGCGGANCGC TATCAGGACA TAGCGTTGGC TACNCGTGAT ATTGCTGAAG
  841 ANCTTGGCGG CGAANGNNCT GACCGCTTCC TCGNGCTTTA CNGTATCGCC GCTCCCGATT
  901 NNCAGCGCAT CGCCTTCNAT NGCCTTCTTG NNNANTTCTN CTGANCGGGN NTCTGGGGTN
  961 NNAAATGACN NANCNAGCGN NGCCNNANCN NGNCNTCNCG ANATTNCNAN TNNNNNNNCN
1021 NNNNTNNNAN NAANNNNNNG NNNTNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl