Resource data sheet
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Human HLA-B*38:01 cDNA

Human HLA-B*38:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03370
Resource name Human HLA-B*38:01 cDNA
Alternative name HLA-B*3801
Clone info. Human HLA-B*38:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03370 Human HLA-B*38:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03370z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB03370_A3Ihp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB03370_A3Iha.seq
>03370_03370_A3Ih_T7_20bp_F03_18.ab1
    1 CGTTCCATCC GTTTTAAACT TAAGCTTCAC CCGGACTCAG AATCTCCTCA GACGCCGAGA
   61 TGCTGGTCAT GGCGCCCCGA ACCGTCCTCC TGCTGCTCTC GGCGGCCCTG GCCCTGACCG
  121 AGACCTGGGC CGGCTCCCAC TCCATGAGGT ATTTCTACAC CTCCGTGTCC CGGCCCGGCC
  181 GCGGGGAGCC CCGCTTCATC TCAGTGGGCT ACGTGGACGA CACGCAGTTC GTGAGGTTCG
  241 ACAGCGACGC CGCGAGTCCG AGAGAGGAGC CGCGGGCGCC GTGGATAGAG CAGGAGGGGC
  301 CGGAATATTG GGACCGGAAC ACACAGATCT GCAAGACCAA CACACAGACT TACCGAGAGA
  361 ACCTGCGGAT CGCGCTCCGC TACTACAACC AGAGCGAGGC CGGGTCTCAC ACCCTCCAGA
  421 GGATGTACGG CTGCGACGTG GGGCCGGACG GGCGCCTCCT CCGCGGGCAT AACCAGTTCG
  481 CCTACGACGG CAAGGATTAC ATCGCCCTGA ACGAGGACCT GAGCTCCTGG ACCGCGGCGG
  541 ACACCGCGGC TCAGATCACC CAGCGCAAGT GGGAGGCGGC CCGTGTGGCG GAGCAGCTGA
  601 GAACCTACCT GGAGGGCACG TGCGTGGAGT GGCTCCGCAG ATACCTGGAG AACGGGAAGG
  661 AGACGCTGCA GCGCGCGGAC CCCCCAAAGA CACATGTGAC CCACCACCCC ATCTCTGACC
  721 ATGAGGCCAC CCTGAGGTGC TGGGCCCTGG GCTTCTACCC TGCGGAGATC ACACTGACCT
  781 GGCAGCGGGA TGGCGAGGAC CAAACTCAGG ACACCGAGCT TGTGGAGACC AGACCAGCAG
  841 GAGACAGAAC CTTCCAGAAA GTGGGCAGCT GTGGTGGTGC CTTCTGGAGA AGAGCAGAGA
  901 TACACATGCC ATGTACAGCA TGAGGGGCTG CCGAAGCCCC TCACCCTGAG ATGGGAGCCA
  961 TCTTCCCAGT CCACCGTCCC CATCGTGGGC ATTGTTGCTG GGCCTGGCTG TCCTAGCAGT
 1021 TGTGGTCATC GGAGCTGTGG TCGCTGCTGT GATGTGTAGG AAGAAGAGTT CAGGTGCAAA
 1081 AGGAAGGGAG CTACTCTCAG CTGCGTCCAG CGAACAGTGC CAGGCTCTGA TGTGTTCTCT
 1141 CACAAGCTTT GAAAAAGCCT TGAGAAACAG CGGGTTCTTT GTGTGGAAGG GAGAACCTTT
1201 GA
//
Primer: BGH_rev RDB03370_A3Ihb.seq
>03370_03370_A3Ih_BGHrev_G03_21.ab1
    1 CTTCTACGGG GTCTCTAGAC TCGAGCGGCC GCATCTCAGT CCCTCACAAG ACAGCTGTCT
   61 CAGGCTTTTC AAGCTGTGAG AGACACATCA GAGCCCTGGG CACTGTCGCT GGACGCAGCC
  121 TGAGAGTAGC TCCCTCCTTT TCCACCTGAA CTCTTCCTCC TACACATCAC AGCAGCGACC
  181 ACAGCTCCGA TGACCACAAC TGCTAGGACA GCCAGGCCAG CAACAATGCC CACGATGGGG
  241 ACGGTGGACT GGGAAGATGG CTCCCATCTC AGGGTGAGGG GCTTCGGCAG CCCCTCATGC
  301 TGTACATGGC ATGTGTATCT CTGCTCTTCT CCAGAAGGCA CCACCACAGC TGCCCACTTC
  361 TGGAAGGTTC TGTCTCCTGC TGGTCTGGTC TCCACAAGCT CGGTGTCCTG AGTTTGGTCC
  421 TCGCCATCCC GCTGCCAGGT CAGTGTGATC TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC
  481 CTCAGGGTGG CCTCATGGTC AGAGATGGGG TGGTGGGTCA CATGTGTCTT TGGGGGGTCC
  541 GCGCGCTGCA GCGTCTCCTT CCCGTTCTCC AGGTATCTGC GGAGCCACTC CACGCACGTG
  601 CCCTCCAGGT AGGTTCTCAG CTGCTCCGCC ACACGGGCCG CCTCCCACTT GCGCTGGGTG
  661 ATCTGAGCCG CGGTGTCCGC CGCGGTCCAG GAGCTCAGGT CCTCGTTCAG GGCGATGTAA
  721 TCCTTGCCGT CGTAGGCGAA CTGGTTATGC CCGCGGAGGA GGCGCCCGTC CGGCCCCACG
  781 TCGCAGCCGT ACATCCTCTG GAGGGTGTGA GACCCGGCCT CGCTCTGGTT GTAGTAGCGG
  841 AGCGCGATCC GCAGGTTCTC TCGGTAAGTC TGTGTGTTGG TCTTGCAGAT CTGTGTGTTC
  901 CGGTCCCAAT ATTCCGGCCC CTCCTGCTCT ATCCACGGCG CCCGCGGCTC CTCTCTCGGA
  961 CTCGCGGCGT CGCTGTCGAA CCTCACGAAC TGCGTGTCGT CCACGTAGCC CACTGAGATG
 1021 AAGCGGGGCT CCCCGCGGGC CGGGCCGGGA CACGGAAGGT GTAGAAAATA CCTCATGGAA
 1081 GTGGAGCCCG GGCCCAGGTC TCGGGTCAGG ACCAGGCCGC CCGAGAGCCA GCAGAGGACG
 1141 ATCGGGGCGC CATGACCAGG CATTCCTTCC GGGCGGTCTC GTGGAAGGGG AAGAAATTCT
//
Primer: SV40pro_F RDB03370_A3Ihc.seq
>03370_03370_A3Ih_SV40pro_F_H02_23.ab1
    1 CACGTCTGGA CATCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
  121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
  661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  781 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
  841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCCTT
  901 CTTGACGAGT TCTTCTGAGC GGGACTCTTG GGGGTTCGAA ATGACCGACC AAGCGACGCC
  961 CCAACCTGCC CATCACGGAG ATTTCGATTT CCCACGCCGT CTTCCTATGA AGGCTGGCTT
 1021 CCGAAATCGT TTTCCCGGAA CGCGGGCTGA TGAATCTTCA GCTGCGGGAT CTCAATGCCT
1081 GGTAGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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