Resource data sheet
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Human HLA-B*08:01 cDNA

Human HLA-B*08:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03369
Resource name Human HLA-B*08:01 cDNA
Alternative name HLA-B*0801
Clone info. Human HLA-B*08:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03369 Human HLA-B*08:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03369z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13691_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13691_A5Jga.seq
>03369_13691_A5Jg_CMV-Forward_C06_09.ab1
    1 CCTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTCACCC GGACTCAGAA
  181 TCTCCTCAGA CGCCGAGATG CTGGTCATGG CGCCCCGAAC CGTCCTCCTG CTGCTCTCGG
  241 CGGCCCTGGC CCTGACCGAG ACCTGGGCCG GCTCCCACTC CATGAGGTAT TTCGACACCG
  301 CCATGTCCCG GCCCGGCCGC GGGGAGCCCC GCTTCATCTC AGTGGGCTAC GTGGACGACA
  361 CGCAGTTCGT GAGGTTCGAC AGCGACGCCG CGAGTCCGAG AGAGGAGCCG CGGGCGCCGT
  421 GGATAGAGCA GGAGGGGCCG GAGTATTGGG ACCGGAACAC ACAGATCTTC AAGACCAACA
  481 CACAGACTGA CCGAGAGAGC CTGCGGAACC TGCGCGGCTA CTACAACCAG AGCGAGGCCG
  541 GGTCTCACAC CCTCCAGAGC ATGTACGGCT GCGACGTGGG GCCGGACGGG CGCCTCCTCC
  601 GCGGGCATAA CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAC GAGGACCTGC
  661 GCTCCTGGAC CGCGGCGGAC ACCGCGGCTC AGATCACCCA GCGCAAGTGG GAGGCGGCCC
  721 GTGTGGCGGA GCAGGACAGA GCCTACCTGG AGGGCACGTG CGTGGAGTGG CTCCGCAGAT
  781 ACCTGGAGAA CGGGAAGGAC ACGCTGGAGC GCGCGGACCC CCCAAAGACA CACGTGACCC
  841 ACCACCCCAT CTCTGACCAT GAGGCCACCC TGAGGTGCTG GGCCCTGGGC TTCTACCCTG
  901 CGGAGATCAC ACTGACCTGG CAGCGGGATG GCGAGGACCA AACTCAGGAC ACTGAGCTTG
  961 TGGAGACCAG ACCAGCAGGA GATAGAACCC TTCCAGAAGT GGGCAGCTGT GGTGTGCCTT
 1021 CTGGAGAGAG CAGAGAATAC ACATGCCATG TACAGCATGA GGGCTGCGAG CCCCTCACCT
1081 GAGATGGAGC CGTCTCCAGT TCACGGTCCC CCATCGGGGC CAATTGTTTG CCTTGC
//
Primer: BGH_rev RDB13691_A5Jgb.seq
>03369_13691_A5Jg_BGHrev_D06_12.ab1
    1 GTTCGGGGCT CTAGACTCGA GCGGCCGCAT CTCAGTCCCT CACAAGACAG CTGTCTCAGG
   61 CTTTTCAAGC TGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCGCTGCAC GCAGCCTGAG
  121 AGTAGCTCCC TCCTTTTCCA CCTGAGCTCT TCCTCCTACA CATCACAGCA GCGACCACAG
  181 CTCCGATGAC CACAACTGCT AGGACAGCCA GGCCAGCAAC AATGCCCACG ATGGGGACGG
  241 TGGACTGGGA AGACGGCTCC CATCTCAGGG TGAGGGGCTT CGGCAGCCCC TCATGCTGTA
  301 CATGGCATGT GTATCTCTGC TCTTCTCCAG AAGGCACCAC CACAGCTGCC CACTTCTGGA
  361 AGGTTCTATC TCCTGCTGGT CTGGTCTCCA CAAGCTCAGT GTCCTGAGTT TGGTCCTCGC
  421 CATCCCGCTG CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC CAGCACCTCA
  481 GGGTGGCCTC ATGGTCAGAG ATGGGGTGGT GGGTCACGTG TGTCTTTGGG GGGTCCGCGC
  541 GCTCCAGCGT GTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT
  601 CCAGGTAGGC TCTGTCCTGC TCCGCCACAC GGGCCGCCTC CCACTTGCGC TGGGTGATCT
  661 GAGCCGCGGT GTCCGCCGCG GTCCAGGAGC GCAGGTCCTC GTTCAGGGCG ATGTAATCCT
  721 TGCCGTCGTA GGCGTACTGG TTATGCCCGC GGAGGAGGCG CCCGTCCGGC CCCACGTCGC
  781 AGCCGTACAT GCTCTGGAGG GTGTGAGACC CGGCCTCGCT CTGGTTGTAG TAGCCGCGCA
  841 GGTTCCGCAG GCTCTCTCGG TCAGTCTGTG TGTTGGTCTT GAAGATCTGT GTGTTCCGGT
  901 CCCAATACTC CGGCCCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCTCT CTCGGACTCG
  961 CGGGCGTCGC TGTCGAACCC TCACGAACTG CGTGTCGTCC ACGTAGCCCA CTGAGATGAA
 1021 GCGGGGGCTC CCCGCGGGCC GGGCCGGGAA CATGGGCGGT GTCGAAATAC CTCATGGAAG
 1081 TGGGGAGCGG GCCAAGATCT CGGTCAGGGT CATGGACGGT CGTAGAGCAG CAGAGAAACG
1141 GATTCTCGGT GGGGGC
//
Primer: SV40pro_F_V2 RDB13691_A5Jgc.seq
>03369_13691_A5Jg_SV40pro_F_V2_E06_15.ab1
    1 GTAGGCTTTT TTGGAGCCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
   61 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
  121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
  181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
  241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
  301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
  361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
  421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
  481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
  541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
  601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
  661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
  721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
  781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
  841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
  901 CTGGGGTTCG AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA
  961 CCGCCGCCTT CTATGAAAAG GTTGGGCTTT CGGAATCGTT TTCCGGGACG CCGGCTGATG
 1021 ATCCTCCAGC GGCGGGGATC TCATGCTGAG TTCTTCGCCA ACCCCGACCT TGTTTATTGC
1081 AGCTTATATG GTTACCAATA CGCCATAAGC ATCCACAATT TTTCCACCAA AGTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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