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Human HLA-B*08:01 cDNA

Human HLA-B*08:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03369
Resource name Human HLA-B*08:01 cDNA
Alternative name HLA-B*0801
Clone info. Human HLA-B*08:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          RDB03369 Human HLA-B*08:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB03369z.seq

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13691_A5Jgp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert5'
          Sequence file: RDB13691_A5Jgd.seq check
          >03369_13691_A5Jg_CMV-Forward_C06_09.ab1
              1 CCTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTCACCC GGACTCAGAA
            181 TCTCCTCAGA CGCCGAGATG CTGGTCATGG CGCCCCGAAC CGTCCTCCTG CTGCTCTCGG
            241 CGGCCCTGGC CCTGACCGAG ACCTGGGCCG GCTCCCACTC CATGAGGTAT TTCGACACCG
            301 CCATGTCCCG GCCCGGCCGC GGGGAGCCCC GCTTCATCTC AGTGGGCTAC GTGGACGACA
            361 CGCAGTTCGT GAGGTTCGAC AGCGACGCCG CGAGTCCGAG AGAGGAGCCG CGGGCGCCGT
            421 GGATAGAGCA GGAGGGGCCG GAGTATTGGG ACCGGAACAC ACAGATCTTC AAGACCAACA
            481 CACAGACTGA CCGAGAGAGC CTGCGGAACC TGCGCGGCTA CTACAACCAG AGCGAGGCCG
            541 GGTCTCACAC CCTCCAGAGC ATGTACGGCT GCGACGTGGG GCCGGACGGG CGCCTCCTCC
            601 GCGGGCATAA CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAC GAGGACCTGC
            661 GCTCCTGGAC CGCGGCGGAC ACCGCGGCTC AGATCACCCA GCGCAAGTGG GAGGCGGCCC
            721 GTGTGGCGGA GCAGGACAGA GCCTACCTGG AGGGCACGTG CGTGGAGTGG CTCCGCAGAT
            781 ACCTGGAGAA CGGGAAGGAC ACGCTGGAGC GCGCGGACCC CCCAAAGACA CACGTGACCC
            841 ACCACCCCAT CTCTGACCAT GAGGCCACCC TGAGGTGCTG GGCCCTGGGC TTCTACCCTG
            901 CGGAGATCAC ACTGACCTGG CAGCGGGATG GCGAGGACCA AACTCAGGAC ACTGAGCTTG
            961 TGGAGACCAG ACCAGCAGGA GATAGAACCC TTCCAGAAGT GGGCAGCTGT GGTGTGCCTT
           1021 CTGGAGAGAG CAGAGAATAC ACATGCCATG TACAGCATGA GGGCTGCGAG CCCCTCACCT
          1081 GAGATGGAGC CGTCTCCAGT TCACGGTCCC CCATCGGGGC CAATTGTTTG CCTTGC
          //
          Primer: BGH_rev2
          Region: insert3'
          Sequence file: RDB13691_A5Jge.seq check
          >03369_13691_A9L2F_BGH_rev2_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNCNNNGCTN NNNNNNNGGN NTTAAACGGG CCCTCTAGAC TCGAGCGGCC
             61 GCATCTCAGT CCCTCACAAG ACAGCTGTCT CAGGCTTTTC AAGCTGTGAG AGACACATCA
            121 GAGCCCTGGG CACTGTCGCT GCACGCAGCC TGAGAGTAGC TCCCTCCTTT TCCACCTGAG
            181 CTCTTCCTCC TACACATCAC AGCAGCGACC ACAGCTCCGA TGACCACAAC TGCTAGGACA
            241 GCCAGGCCAG CAACAATGCC CACGATGGGG ACGGTGGACT GGGAAGACGG CTCCCATCTC
            301 AGGGTGAGGG GCTTCGGCAG CCCCTCATGC TGTACATGGC ATGTGTATCT CTGCTCTTCT
            361 CCAGAAGGCA CCACCACAGC TGCCCACTTC TGGAAGGTTC TATCTCCTGC TGGTCTGGTC
            421 TCCACAAGCT CAGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT CAGTGTGATC
            481 TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC AGAGATGGGG
            541 TGGTGGGTCA CGTGTGTCTT TGGGGGGTCC GCGCGCTCCA GCGTGTCCTT CCCGTTCTCC
            601 AGGTATCTGC GGAGCCACTC CACGCACGTG CCCTCCAGGT AGGCTCTGTC CTGCTCCGCC
            661 ACACGGGCCG CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CGGTGTCCGC CGCGGTCCAG
            721 GANCGCANGT CCTCGTTCNG GGCGATGTAA TCCTTGCCGT CGTANGCGTA CTGGTTATGC
            781 CCGCNGAGGA NGCGCCCGNC CGGNCCCNCG TCNCAGCCGT ACATGCTCTG GANGGTGTGA
            841 NANCCGGCCT CGCNCNGGTT GTANTANCCG CNCAGGNNCC GCANGCTCTC TCGGTCNGTC
            901 TGTGTGTNNG TNTNNNNNAT NNGTGNGTTC CGGTCCCANN ACTCNNGCCC NNNNNNNNNN
          //
          Primer: SV40pro_F_V2
          Region: NeoR
          Sequence file: RDB13691_A5Jgf.seq check
          >03369_13691_A5Jg_SV40pro_F_V2_E06_15.ab1
              1 GTAGGCTTTT TTGGAGCCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
             61 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
            121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
            181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
            241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
            301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
            361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
            421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
            481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
            541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
            601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
            661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
            721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
            781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
            841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
            901 CTGGGGTTCG AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA
            961 CCGCCGCCTT CTATGAAAAG GTTGGGCTTT CGGAATCGTT TTCCGGGACG CCGGCTGATG
           1021 ATCCTCCAGC GGCGGGGATC TCATGCTGAG TTCTTCGCCA ACCCCGACCT TGTTTATTGC
          1081 AGCTTATATG GTTACCAATA CGCCATAAGC ATCCACAATT TTTCCACCAA AGTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl