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Human HLA-A*01:01 cDNA

Human HLA-A*01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03368
Resource name Human HLA-A*01:01 cDNA
Alternative name HLA-A*0101
Clone info. Human HLA-A*01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB03368 Human HLA-A*01:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB03368z.seq

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14970_A2Kip1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB14970_A7E1d.seq check
          >03368_14970_B0GhF_CMV-Forward_F08_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTNNN NGCNNTGGGC GGTANGCGTG TACGGNGGGN NGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGAT TCTCCCCAGA
            181 CGCCGAGGAT GGCCGTCATG GCGCCCCGAA CCCTCCTCCT GCTACTCTCG GGGGCCCTGG
            241 CCCTGACCCA GACCTGGGCG GGCTCCCACT CCATGAGGTA TTTCTTCACA TCCGTGTCCC
            301 GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CCGTGGGCTA CGTGGACGAC ACGCAGTTCG
            361 TGCGGTTCGA CAGCGACGCC GCGAGCCAGA AGATGGAGCC GCGGGCGCCG TGGATAGAGC
            421 AGGAGGGGCC GGAGTATTGG GACCAGGAGA CACGGAATAT GAAGGCCCAC TCACAGACTG
            481 ACCGAGCGAA CCTGGGGACC CTGCGCGGCT ACTACAACCA GAGCGAGGAC GGTTCTCACA
            541 CCATCCAGAT AATGTATGGC TGCGACGTGG GGCCGGACGG GCGCTTCCTC CGCGGGTACC
            601 GGCAGGACGC CTACGACGGC AAGGNTTACA TCGCCCTGAA CGAGGACCTG CGCTCTTGGA
            661 CCGCGGCGGA CATGGCAGCT CAGATCACCA AGCGCAAGTG GGANGCGGTC CATGCGGCGG
            721 ANCAGCGGAN AGTCTACCTG GAGGGCCGGT GCGTGGACGG GCTCCGCANA TACCTGGANA
            781 ACGGGAANGA NANGCTGCAG CGCACGGNNC CCCCCCAAGA NACATATGAN CCACCACNNC
            841 ATCTCNGNNC NTNNNGCCNC CCNNNNGNGC NNGNCCCNNG NNTTCNACCN NGNNGNNNTC
          901 ACNNNNNNNN NN
          //
          Primer: BGH_rev2
          Region: bGH pA, insert 3'
          Sequence file: RDB14970_A7E1e.seq check
          >03368_14970_B0GhF_BGH_rev2_A08_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGGG GNNGGNNNNN NACAGATGGC TGGCAACTAG AANGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCA CAAGGCAGCT
            121 GTCTCACACT TTACAAGCTG TGAGAGACAC ATCAGAGCCC TGGGCACTGT CACTGCTTGC
            181 AGCCTGAGTG TAACTCCCTC CTTTTCTATC TGAGCTCTTC CTCCTCCACA TCACGGCAGC
            241 GACCACAGCT CCAGTGATCA CAGCTCCAAG GAGAACCAGG CCAGCAATGA TGCCCACGAT
            301 GGGGATGGTG GGCTGGGAAG ACAGCTCCCA TCTCAGGGTG AGGGGCTTGG GCAGACCCTC
            361 ATGCTGCACA TGGCAGGTGT ATCTCTGCTC CTCTCCAGAA GGCACCACCA CAGCCGCCCA
            421 CTTCTGGAAG GTTCCATCCC CTGCAGGCCT GGTCTCCACG AGCTCCGTGT CCTGGGTCTG
            481 GTCCTCCCCA TCCCGCTGCC AGGTCAGTGT GATCTCCGCA GGGTAGAAGC CCAGGGCCCA
            541 GCACCTCAGG GTGGCCTCAT GGTCAGAGAT GGGGTGGTGG GTCATATGTG TCTTGGGGGG
            601 GTCCGTGCGC TGCAGCGTCT CCTTCCCGTT CTCCAGGTAT CTGCGGANCC CGTCCACGCA
            661 CCGGCCCTCC AGGTAGACTC TCCGCTGCTC CGCCGCATGG ACCGCCTCCC ACTTGCGCTT
            721 GGTGATCTGA NCTGCCATGT CCGNNGCGGN CCAAGANCGC ANGNCCTCGT TCANGGCGAT
            781 GTAATCCTNG CCGTCNNNNN NNCCTGCCNG NACCCGNNGA NGNANCGCCN GNCCGGNCCN
          841 ACNNCNNANC CNTACNTTAT CNNGNNNGNN GTGANNNNNN NNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB14970_A7E1f.seq check
          >03368_14970_B0GhF_SV40pro_ori_F_B08_05_ABI24.ab1
              1 NNNNNNNNNN NNNNGCCGCC NCTGCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT
             61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
            121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
            181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
            241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
            301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
            361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
            421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
            481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
            541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
            601 GTCTTGTCGA TCAGGATGAT CTGGACGAAN AGCATCAGGG GCTCGCGCCA GCCGAACTGT
            661 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGANGNNCT CGTCGTGACC CATGGCGATG
            721 CCTGCTNGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACNGNGGNN
            781 GGCTGGGNGT GGCGGNNCGC TATCNNNACN TANNGTTGGC NACCNNNGAT ANNGCNNAAG
            841 ANCTNNGNGG NNAANNGNNN NNNNNCTNCC NCNNNCTTNN NNNNATCGNN GCNNCNNNNN
          901 NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl