Resource data sheet
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Human HLA-A*01:01 cDNA

Human HLA-A*01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03368
Resource name Human HLA-A*01:01 cDNA
Alternative name HLA-A*0101
Clone info. Human HLA-A*01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03368 Human HLA-A*01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03368z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB03368_A2Kip1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_long RDB03368_A2Kia.seq
>03368_03368_A2Ki_T7Long_G09_21.ab1
    1 GAAAGGGCCT TGGGCTAGCG TTTAACTTAA GCTTGATTCT CCCCAGACGC CGAGGATGGC
   61 CGTCATGGCG CCCCGAACCC TCCTCCTGCT ACTCTCGGGG GCCCTGGCCC TGACCCAGAC
  121 CTGGGCGGGC TCCCACTCCA TGAGGTATTT CTTCACATCC GTGTCCCGGC CCGGCCGCGG
  181 GGAGCCCCGC TTCATCGCCG TGGGCTACGT GGACGACACG CAGTTCGTGC GGTTCGACAG
  241 CGACGCCGCG AGCCAGAAGA TGGAGCCGCG GGCGCCGTGG ATAGAGCAGG AGGGGCCGGA
  301 GTATTGGGAC CAGGAGACAC GGAATATGAA GGCCCACTCA CAGACTGACC GAGCGAACCT
  361 GGGGACCCTG CGCGGCTACT ACAACCAGAG CGAGGACGGT TCTCACACCA TCCAGATAAT
  421 GTATGGCTGC GACGTGGGGC CGGACGGGCG CTTCCTCCGC GGGTACCGGC AGGACGCCTA
  481 CGACGGCAAG GATTACATCG CCCTGAACGA GGACCTGCGC TCTTGGACCG CGGCGGACAT
  541 GGCAGCTCAG ATCACCAAGC GCAAGTGGGA GGCGGTCCAT GCGGCGGAGC AGCGGAGAGT
  601 CTACCTGGAG GGCCGGTGCG TGGACGGGCT CCGCAGATAC CTGGAGAACG GGAAGGAGAC
  661 GCTGCAGCGC ACGGACCCCC CCAAGACACA TATGACCCAC CACCCCATCT CTGACCATGA
  721 GGCCACCCTG AGGTGCTGGG CCCTGGGCTT CTACCCTGCG GAGATCACAC TGACCTGGCA
  781 GCGGGATGGG GAGGACCAGA CCCAGGACAC GGAGCTCGTG GAGACCAGGC CTGCAGGGGA
  841 TGGAACCTTC CAGAAGTGGG CGGCTGTGGT GGTGCCTTCT GGAGAGGAGC AGAGATACAC
  901 CTGCCATGTG CAGCATGAGG GTCTGCCCAA GCCCCTCACC CTGAGATGGG AGCTGTCTTC
  961 CCAGCCCACC ATCCCCATCG TGGGCATCAT TGCTGGGCCT GGTTCTCCTT GGAGCTGTGA
 1021 TCACTGGAGC TGTGGTCGCT GCCGTGATGT GAGAGAAGAG CTCAGAATAG AAAAAGAGGG
 1081 AGTTTACACC TCAAGCTGCA GCAGTGACCA GTGCCAGCTC TGGAATGGTT CTTCCTCACA
1141 GCCATGTAAG GTGTGAGACA CAGCCTGTCC TTGTGTGCGG AGCGCT
//
Primer: BGH_rev RDB03368_A2Kib.seq
>03368_03368_A2Ki_BGHrev_H09_24.ab1
    1 ATTTTATCGG GGTCCTCTAG ACTCGAGCGG CCGCACAAGG CAGCTGTCTC ACACTTTACA
   61 AGCTGTGAGA GACACATCAG AGCCCTGGGC ACTGTCACTG CTTGCAGCCT GAGTGTAACT
  121 CCCTCCTTTT CTATCTGAGC TCTTCCTCCT CCACATCACG GCAGCGACCA CAGCTCCAGT
  181 GATCACAGCT CCAAGGAGAA CCAGGCCAGC AATGATGCCC ACGATGGGGA TGGTGGGCTG
  241 GGAAGACAGC TCCCATCTCA GGGTGAGGGG CTTGGGCAGA CCCTCATGCT GCACATGGCA
  301 GGTGTATCTC TGCTCCTCTC CAGAAGGCAC CACCACAGCC GCCCACTTCT GGAAGGTTCC
  361 ATCCCCTGCA GGCCTGGTCT CCACGAGCTC CGTGTCCTGG GTCTGGTCCT CCCCATCCCG
  421 CTGCCAGGTC AGTGTGATCT CCGCAGGGTA GAAGCCCAGG GCCCAGCACC TCAGGGTGGC
  481 CTCATGGTCA GAGATGGGGT GGTGGGTCAT ATGTGTCTTG GGGGGGTCCG TGCGCTGCAG
  541 CGTCTCCTTC CCGTTCTCCA GGTATCTGCG GAGCCCGTCC ACGCACCGGC CCTCCAGGTA
  601 GACTCTCCGC TGCTCCGCCG CATGGACCGC CTCCCACTTG CGCTTGGTGA TCTGAGCTGC
  661 CATGTCCGCC GCGGTCCAAG AGCGCAGGTC CTCGTTCAGG GCGATGTAAT CCTTGCCGTC
  721 GTAGGCGTCC TGCCGGTACC CGCGGAGGAA GCGCCCGTCC GGCCCCACGT CGCAGCCATA
  781 CATTATCTGG ATGGTGTGAG AACCGTCCTC GCTCTGGTTG TAGTAGCCGC GCAGGGTCCC
  841 CAGGTTCGCT CGGTCAGTCT GTGAGTGGGC CTTCATATTC CGTGTCTCCT GGTCCCAATA
  901 CTCCGGCCCC TCCTGCTCTA TCCACGGCGC CCGCGGCTCC ATCTTCTGGC TCGCGGCGTC
  961 GCTGTCGAAC CGCACGAACT GCGTGTCGTC CACGTAGCCC ACGGGCGATG AAGCGGGGCT
 1021 CCCCGCGGGC CGGCCGGGAC ACGGATGTGA AGAAAATACC TCATGGAGTG GGAGCCCGCC
 1081 CAGGGTCTGG GGTCAGATAG CTCCGAGAGT AGCAGAGGAA GTTCCGGGCG CATGACCGCC
1141 ATCCTCGGCG TCCTGGGGGG AGAAATCCAG CGTTAAGGTT TAAACAC
//
Primer: SV40pro_F RDB03368_A2Kic.seq
>03368_03368_A2Ki_SV40pro_F_C11_08_ABI24.ab1
    1 CCTGTCTGGA CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  961 AACCTGCCCA TCACGAGATT TCGATTCCCA CCGCCGCCTT CTATGAAAGT TGGGCTTTCG
 1021 GAATCGTTTT CTGGACGCGC TGGATGATTC CTCAGCGCGG GATCTCATGC TGGAAGTCTC
1081 GCCACCCCGA CCTTGCTTAT TGCCAGCTTA TAATTGGCTT CA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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