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Human HLA-A*03:01 cDNA

Human HLA-A*03:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03367
Resource name Human HLA-A*03:01 cDNA
Alternative name HLA-A*0301
Clone info. Human HLA-A*03:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          RDB03367 Human HLA-A*03:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB03367z.seq

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13684_A5Jgp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert5'
          Sequence file: RDB13684_A5Jgd.seq check
          >03367_13684_A5Jg_CMV-Forward_H05_23.ab1
              1 CGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTGATTCT CCCCAGACGC
            181 CGAGGATGGC CGTCATGGCG CCCCGAACCC TCCTCCTGCT ACTCTCGGGG GCCCTGGCCC
            241 TGACCCAGAC CTGGGCGGGC TCCCACTCCA TGAGGTATTT CTTCACATCC GTGTCCCGGC
            301 CCGGCCGCGG GGAGCCCCGC TTCATCGCCG TGGGCTACGT GGACGACACG CAGTTCGTGC
            361 GGTTCGACAG CGACGCCGCG AGCCAGAGGA TGGAGCCGCG GGCGCCGTGG ATAGAGCAGG
            421 AGGGGCCGGA GTATTGGGAC CAGGAGACAC GGAATGTGAA GGCCCAGTCA CAGACTGACC
            481 GAGTGGACCT GGGGACCCTG CGCGGCTACT ACAACCAGAG CGAGGCCGGT TCTCACACCA
            541 TCCAGATAAT GTATGGCTGC GACGTGGGGT CGGACGGGCG CTTCCTCCGC GGGTACCGGC
            601 AGGACGCCTA CGACGGCAAG GATTACATCG CCCTGAACGA GGACCTGCGC TCTTGGACCG
            661 CGGCGGACAT GGCGGCTCAG ATCACCAAGC GCAAGTGGGA GGCGGCCCAT GAGGCGGAGC
            721 AGTTGAGAGC CTACCTGGAT GGCACGTGCG TGGAGTGGCT CCGCAGATAC CTGGAGAACG
            781 GGAAGGAGAC GCTGCAGCGC ACGGACCCCC CCAAGACACA TATGACCCAC CACCCCATCT
            841 CTGACCATGA GGCCACCCTG AGGTGCTGGG CCCTGGGCTT CTACCCTGCG GAGATCACAC
            901 TGACCTGGCA GCGGGATGGG GGAGGACCAG ACCCAGGACA CGGAGCTCGT GGAGACCAGG
            961 CCTGCAGGGA TGGAAACCTT CCAGAAGTGG GCGGCTGTGG TGGTGCCCTT CTGGAGAGGA
           1021 GCAGAGATAC ACCTGCCCAT GTGCAGCATG AGGTCTGCCC AGCCCCTCAC CCTGAGATGG
           1081 GGAGCTGTCT CCAAGCCAAC AATTCCCCAA TCGTGGGCAT CCATTGCCTG GACCTGGTAT
          //
          Primer: BGH_rev2
          Region: bGHp(A),insert3'
          Sequence file: RDB13684_A5Jge.seq check
          >03367_13684_A9L2F_BGH_rev2_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNTTA AACGGGCCCT CTAGACTCGA GCGGCCGCAC
             61 AAGGCAGCTG TCTCACACTT TACAAGCTGT GAGGGACACA TCAGAGCCCT GGGCACTGTC
            121 ACTGCTTGCA GCCTGAGTGT AACTCCCTCC TTTTCTATCT GAGCTCTTCC TCCTCCACAT
            181 CACGGCAGCG ACCACAGCTC CAGTGATCAC AGCTCCAAGG AGAACCAGGC CAGCAATGAT
            241 GCCCACGATG GGGATGGTGG GCTGGGAAGA CAGCTCCCAT CTCAGGGTGA GGGGCTTGGG
            301 CAGACCCTCA TGCTGCACAT GGCAGGTGTA TCTCTGCTCC TCTCCAGAAG GCACCACCAC
            361 AGCCGCCCAC TTCTGGAAGG TTCCATCCCC TGCAGGCCTG GTCTCCACGA GCTCCGTGTC
            421 CTGGGTCTGG TCCTCCCCAT CCCGCTGCCA GGTCAGTGTG ATCTCCGCAG GGTAGAAGCC
            481 CAGGGCCCAG CACCTCAGGG TGGCCTCATG GTCAGAGATG GGGTGGTGGG TCATATGTGT
            541 CTTGGGGGGG TCCGTGCGCT GCAGCGTCTC CTTCCCGTTC TCCAGGTATC TGCGGAGCCA
            601 CTCCACGCAC GTGCCATCCA GGTAGGCTCT CAACTGCTCC GCCTCATGGG CCGCCTCCCA
            661 CTTGCGCTTG GTGATCTGAG CCGCCATGTC CGCCGCGGTC CANNNANCGC NNGTCCTCGT
            721 TCAGGGCGAT GTAATCCTTG CCGTCGTNNN GTCCTGCCGG TACCCGCGGA GNANNNNCCC
            781 GTCCNANCCC ACGTCNNAGC CATACNTTAT CNGGANGGTG TGANNNNNNG NCTCNCTCNN
            841 GTNNTANNNN NNNCGNNNNG GNNCCNNNNN NNNNTCNNNN CANNCTGTGN NNTNGGGNCN
          901 TNNNNATTNN NGNGTCNCCN NGNNNCCANN NANNNNNNNN CNTNCNNNNN NNNNN
          //
          Primer: SV40pro_F_V2
          Region: NeoR
          Sequence file: RDB13684_A5Jgf.seq check
          >03367_13684_A5Jg_SV40pro_F_V2_B06_06.ab1
              1 GACCACTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
             61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
            121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
            181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
            241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
            301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
            361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
            421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
            481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
            541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
            601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
            661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
            721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
            781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
            841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
            901 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC
            961 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CCGGGACGCC CGGCTGGGAT
           1021 GATCCTCCCA GCGCGGGGGA TCTCATGCTG GAGTTCTTCG CCCCACCCCC ACTGTTAATT
           1081 GCAAGCTTAT ATGTTACAAT AAGCATAGCA TCCACCAAAT TTCCACAGTT AAAAAGGCAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl