Resource data sheet
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Human HLA-A*03:01 cDNA

Human HLA-A*03:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03367
Resource name Human HLA-A*03:01 cDNA
Alternative name HLA-A*0301
Clone info. Human HLA-A*03:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03367 Human HLA-A*03:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03367z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13684_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13684_A5Jga.seq
>03367_13684_A5Jg_CMV-Forward_H05_23.ab1
    1 CGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTGATTCT CCCCAGACGC
  181 CGAGGATGGC CGTCATGGCG CCCCGAACCC TCCTCCTGCT ACTCTCGGGG GCCCTGGCCC
  241 TGACCCAGAC CTGGGCGGGC TCCCACTCCA TGAGGTATTT CTTCACATCC GTGTCCCGGC
  301 CCGGCCGCGG GGAGCCCCGC TTCATCGCCG TGGGCTACGT GGACGACACG CAGTTCGTGC
  361 GGTTCGACAG CGACGCCGCG AGCCAGAGGA TGGAGCCGCG GGCGCCGTGG ATAGAGCAGG
  421 AGGGGCCGGA GTATTGGGAC CAGGAGACAC GGAATGTGAA GGCCCAGTCA CAGACTGACC
  481 GAGTGGACCT GGGGACCCTG CGCGGCTACT ACAACCAGAG CGAGGCCGGT TCTCACACCA
  541 TCCAGATAAT GTATGGCTGC GACGTGGGGT CGGACGGGCG CTTCCTCCGC GGGTACCGGC
  601 AGGACGCCTA CGACGGCAAG GATTACATCG CCCTGAACGA GGACCTGCGC TCTTGGACCG
  661 CGGCGGACAT GGCGGCTCAG ATCACCAAGC GCAAGTGGGA GGCGGCCCAT GAGGCGGAGC
  721 AGTTGAGAGC CTACCTGGAT GGCACGTGCG TGGAGTGGCT CCGCAGATAC CTGGAGAACG
  781 GGAAGGAGAC GCTGCAGCGC ACGGACCCCC CCAAGACACA TATGACCCAC CACCCCATCT
  841 CTGACCATGA GGCCACCCTG AGGTGCTGGG CCCTGGGCTT CTACCCTGCG GAGATCACAC
  901 TGACCTGGCA GCGGGATGGG GGAGGACCAG ACCCAGGACA CGGAGCTCGT GGAGACCAGG
  961 CCTGCAGGGA TGGAAACCTT CCAGAAGTGG GCGGCTGTGG TGGTGCCCTT CTGGAGAGGA
 1021 GCAGAGATAC ACCTGCCCAT GTGCAGCATG AGGTCTGCCC AGCCCCTCAC CCTGAGATGG
 1081 GGAGCTGTCT CCAAGCCAAC AATTCCCCAA TCGTGGGCAT CCATTGCCTG GACCTGGTAT
//
Primer: BGH_rev RDB13684_A5Jgb.seq
>03367_13684_A5Jg_BGHrev_F09_18.ab1
    1 GTATACGGGT CTCTAGACTC GAGCGGCCGC ACAAGGCAGC TGTCTCACAC TTTACAAGCT
   61 GTGAGGGACA CATCAGAGCC CTGGGCACTG TCACTGCTTG CAGCCTGAGT GTAACTCCCT
  121 CCTTTTCTAT CTGAGCTCTT CCTCCTCCAC ATCACGGCAG CGACCACAGC TCCAGTGATC
  181 ACAGCTCCAA GGAGAACCAG GCCAGCAATG ATGCCCACGA TGGGGATGGT GGGCTGGGAA
  241 GACAGCTCCC ATCTCAGGGT GAGGGGCTTG GGCAGACCCT CATGCTGCAC ATGGCAGGTG
  301 TATCTCTGCT CCTCTCCAGA AGGCACCACC ACAGCCGCCC ACTTCTGGAA GGTTCCATCC
  361 CCTGCAGGCC TGGTCTCCAC GAGCTCCGTG TCCTGGGTCT GGTCCTCCCC ATCCCGCTGC
  421 CAGGTCAGTG TGATCTCCGC AGGGTAGAAG CCCAGGGCCC AGCACCTCAG GGTGGCCTCA
  481 TGGTCAGAGA TGGGGTGGTG GGTCATATGT GTCTTGGGGG GGTCCGTGCG CTGCAGCGTC
  541 TCCTTCCCGT TCTCCAGGTA TCTGCGGAGC CACTCCACGC ACGTGCCATC CAGGTAGGCT
  601 CTCAACTGCT CCGCCTCATG GGCCGCCTCC CACTTGCGCT TGGTGATCTG AGCCGCCATG
  661 TCCGCCGCGG TCCAAGAGCG CAGGTCCTCG TTCAGGGCGA TGTAATCCTT GCCGTCGTAG
  721 GCGTCCTGCC GGTACCCGCG GAGGAAGCGC CCGTCCGACC CCACGTCGCA GCCATACATT
  781 ATCTGGATGG TGTGAGAACC GGCCTCGCTC TGGTTGTAGT AGCCGCGCAG GGTCCCCAGG
  841 TCCACTCGGT CAGTCTGTGA CTGGGCCTTC ACATTCCGTG TCTCCTGGTC CCAATACTCC
  901 GGCCTCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCATC CTCTGGCTCG CGGCGTCGCT
  961 GTCGAACTCG CACGAACTGC GTGTCGTCCA CGTAGCTCCA CGGGCGATGA AGCGGGGCTT
 1021 CCTCCGCGGG CCCGGGCCGG GGACACGGGA TGTGAAGAAA ATACCTCATC AGTGGAGCCC
1081 GTCCTAGTCT GGGATCAGAT CAGGACTCCT CTGAGAGTTA GCCAAGGGAA GGAAAGTG
//
Primer: SV40pro_F_V2 RDB13684_A5Jgc.seq
>03367_13684_A5Jg_SV40pro_F_V2_B06_06.ab1
    1 GACCACTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
   61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
  841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
  901 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC
  961 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CCGGGACGCC CGGCTGGGAT
 1021 GATCCTCCCA GCGCGGGGGA TCTCATGCTG GAGTTCTTCG CCCCACCCCC ACTGTTAATT
 1081 GCAAGCTTAT ATGTTACAAT AAGCATAGCA TCCACCAAAT TTCCACAGTT AAAAAGGCAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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