Resource data sheet
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Human HLA-A*26:03 cDNA

Human HLA-A*26:03 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03366
Resource name Human HLA-A*26:03 cDNA
Alternative name HLA-A*2603
Clone info. Human HLA-A*26:03 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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RDB03366 Human HLA-A*26:03 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03366z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB03366_A3Fap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_long RDB03366_A3Faa.seq
>3366_3366_A3Fa_T7long_A03_03.ab1
    1 GCAAAGGCCT GGGCTAGCGT TTAACTTAAG CTTGATTCTC CCCAGACGCC GAGGATGGCC
   61 GTCATGGCGC CCCGAACCCT CGTCCTGCTA CTCTCGGGGG CCCTGGCCCT GACCCAGACC
  121 TGGGCGGGCT CCCACTCCAT GAGGTATTTC TACACCTCCG TGTCCCGGCC CGGCCGCGGG
  181 GAGCCCCGCT TCATCGCCGT GGGCTACGTG GACGACACGC AGTTCGTGCG GTTCGACAGC
  241 GACGCCGCGA GCCAGAGGAT GGAGCCGCGG GCGCCGTGGA TAGAGCAGGA GGGGCCGGAG
  301 TATTGGGACC GGAACACACG GAATGTGAAG GCCCACTCAC AGACTCACCG AGTGGACCTG
  361 GGGACCCTGC GCGGCTACTA CAACCAGAGC GAGGACGGTT CTCACACCAT CCAGAGGATG
  421 TATGGCTGCG ACGTGGGGCC GGACGGGCGC TTCCTCCGCG GGTACCAGCA GGACGCTTAC
  481 GACGGCAAGG ATTACATCGC CCTGAACGAG GACCTGCGCT CTTGGACCGC GGCGGACATG
  541 GCGGCTCAGA TCACCCAGCG CAAGTGGGAG ACGGCCCATG AGGCGGAGCA GTGGAGAGCC
  601 TACCTGGAGG GCCGGTGCGT GGAGTGGCTC CGCAGATACC TGGAGAACGG GAAGGAGACG
  661 CTGCAGCGCA CGGACGCCCC CAAGACGCAT ATGACTCACC ACGCTGTCTC TGACCATGAG
  721 GCCACCCTGA GGTGCTGGGC CCTGAGCTTC TACCCTGCGG AGATCACACT GACCTGGCAG
  781 CGGGATGGGG AGGACCAGAC CCAGGACACG GAGCTCGTGG AGACCAGGCC TGCAGGGGAT
  841 GGGACCTTCC AGAAGTGGGC GTCTGTGGTG GTGCCTTCTG GACAGGAGCA GAGATACACC
  901 TGCCATGTGC AGCATGAGGG TCTGCCCAAG CCCCTCACCC TGAGATGGGA GCCGTCTTCC
  961 CAGCCCACCA TCCCCATCGT GGGCATCATT GCTGGCCTGG TTTCTCTTTG GAGCTGTGAT
 1021 CGCTGGGAGC TGTGGTCGCT GCTGTGATGT GCAGAGAAGA GCTCAGAATA GAAAAGGAGG
 1081 GAGCTACTCT CAGCTGCAAG CCAGTGACAG TGACCCAATG GCTCCTGAAT ATGTTCTCCT
1141 CACACAGGCC ATTGGTTA
//
Primer: BGH_rev RDB03366_A3Fab.seq
>3366_3366_A3Fa_BGHrev_B03_06.ab1
    1 CTTTACGGTA CCTCTAGACT CGAGCGGCCG CACAAGGCAG CTGTCTCACA CTTTACAAGC
   61 TGTGAGAGAC ATATCAGAGC CCTGGGCACT GTCACTGCTT GCAGCCTGAG AGTAGCTCCC
  121 TCCTTTTCTA TCTGAGCTCT TCCTCCTCCA CATCACAGCA GCGACCACAG CTCCAGCGAT
  181 CACAGCTCCA AAGAGAACCA GGCCAGCAAT GATGCCCACG ATGGGGATGG TGGGCTGGGA
  241 AGACGGCTCC CATCTCAGGG TGAGGGGCTT GGGCAGACCC TCATGCTGCA CATGGCAGGT
  301 GTATCTCTGC TCCTGTCCAG AAGGCACCAC CACAGACGCC CACTTCTGGA AGGTCCCATC
  361 CCCTGCAGGC CTGGTCTCCA CGAGCTCCGT GTCCTGGGTC TGGTCCTCCC CATCCCGCTG
  421 CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCTCAGGGCC CAGCACCTCA GGGTGGCCTC
  481 ATGGTCAGAG ACAGCGTGGT GAGTCATATG CGTCTTGGGG GCGTCCGTGC GCTGCAGCGT
  541 CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACCGGCCCT CCAGGTAGGC
  601 TCTCCACTGC TCCGCCTCAT GGGCCGTCTC CCACTTGCGC TGGGTGATCT GAGCCGCCAT
  661 GTCCGCCGCG GTCCAAGAGC GCAGGTCCTC GTTCAGGGCG ATGTAATCCT TGCCGTCGTA
  721 AGCGTCCTGC TGGTACCCGC GGAGGAAGCG CCCGTCCGGC CCCACGTCGC AGCCATACAT
  781 CCTCTGGATG GTGTGAGAAC CGTCCTCGCT CTGGTTGTAG TAGCCGCGCA GGGTCCCCAG
  841 GTCCACTCGG TGAGTCTGTG AGTGGGCCTT CACATTCCGT GTGTTCCGGT CCCAATACTC
  901 CGGCCCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCATC CTCTGGCTCG CGGCGTCGCT
  961 GTCGAACCGC ACGAACTGCG TGTCGTCCAC GTAGCCCACG GCGATGAAGC GGGGCTCCCC
 1021 GCGGCCCGGG CCGGGACACG GAGGTGTAGA AATACCTCAT GGAGTGGAGC CCGCCCAGGG
 1081 TCTGGGTCAG GGCCAGGGCC CCCGAGAGTT AGCAAGAACG AAGGATCGGG GCGGCATGAC
1141 GGACCAATCC CTTGCGGGCG ATCCTGGGGG GGAG
//
Primer: SV40pro_F_V2 RDB03366_A3Fac.seq
>03366_03366_A3Fa_SV40pro_F_V2_G05_20.ab1
    1 TGGAACTTTT TTTGGAGCCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGT ATATCCATTT
   61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
  121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
  181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
  241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
  301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
  361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
  421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
  481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
  541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
  601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
  661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
  721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
  781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
  841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
  901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC
  961 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGGA
 1021 TGATCCTCCA GCGCGGGGAT CTCATGCTGA GTCTTCGCCC CACCCCCCAA CTTGTTATGC
 1081 CAGCCTTATA ATGGGATACC AATAAAGCCA ATAGCATCAA CAACTTCACA AATAAAGGCA
1141 ATTTTTTTTT G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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