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Human HLA-A*24:20 cDNA

Human HLA-A*24:20 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03365
Resource name Human HLA-A*24:20 cDNA
Alternative name HLA-A*2420
Clone info. Human HLA-A*24:20 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03365 Human HLA-A*24:20 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB03365z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14004_A6D4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14004_A6D4a.seq
>03365_14004_A6D4_CMV-Forward_D10_10.ab1
    1 CTTACTCGCC CATTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA
   61 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC
  121 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGATTCTC CCCAGACGCC
  181 GAGGATGGCC GTCATGGCGC CCCGAACCCT CGTCCTGCTA CTCTCGGGGG CCCTGGCCCT
  241 GACCCAGACC TGGGCAGGCT CCCAATCCAT GAGGTATTTC TCCACATCCG TGTCCCGGCC
  301 CGGCCGCGGG GAGCCCCGCT TCATCGCCGT GGGCTACGTG GACGACACGC AGTTCGTGCG
  361 GTTCGACAGC GACGCCGCGA GCCAGAGGAT GGAGCCGCGG GCGCCGTGGA TAGAGCAGGA
  421 GGGGCCGGAG TATTGGGACG AGGAGACAGG GAAAGTGAAG GCCCACTCAC AGACTGACCG
  481 AGAGAACCTG CGGATCGCGC TCCGCTACTA CAACCAGAGC GAGGCCGGTT CTCACACCCT
  541 CCAGATGATG TTTGGCTGCG ACGTGGGGTC GGACGGGCGC TTCCTCCGCG GGTACCACCA
  601 GTACGCCTAC GACGGCAAGG ATTACATCGC CCTGAAAGAG GACCTGCGCT CTTGGACCGC
  661 GGCGGACATG GCGGCTCAGA TCACCAAGCG CAAGTGGGAG GCGGCCCATG TGGCGGAGCA
  721 GCAGAGAGCC TACCTGGAGG GCACGTGCGT GGACGGGCTC CGCAGATACC TGGAGAACGG
  781 GAAGGAGACG CTGCAGCGCA CGGACCCCCC CAAGACACAT ATGACCCACC ACCCCATCTC
  841 TGACCATGAG GCCACTCTGA GATGCTGGGC CCTGGGCTTC TACCCTGCGG AGATCACACT
  901 GACCTGGCAG CGGGATGGGG GAGGACCAGA CCCAGGACAC GGAGCTTGTG GAGACCAGGC
  961 CTGCACGGGA TGGGAACCCT TCCAGAAGTG GGCAGCTGTG GGTGGGTACC CTTTCTGGGA
 1021 GAGGAGCAGA GATACACCTG CCAATGTGCA GCAATGAAGG TCTGCCCGAG CCCCTCAACC
1081 CTGAGATGGG AGCCATCGTC CCAGGCCACG GTTCCCCAAT TCGATGGGGC ATCATTGGC
//
Primer: BGH_rev2 RDB14004_A6D4b.seq
>03365_14004_A6D4_BGH_rev2_E10_13.ab1
    1 CATCGTAGAG CACGGGGGAG GGGCAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA
   61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCACAA GGCAGCTGTC
  121 TCACACTTTA CAAGCTGTGA GAGACACATC AGAGCCCTGG GCACTGTCAC TGCTTGCAGC
  181 CTGAGAGTAG CTCCCTCCTT TTCTATCTGA GCTGTTCCTC CTCCACATCA CAGCAGCGAC
  241 CACAGCTCCA GTGATCACAG CTCCAAGGAG AACCAGGCCA GCAATGATGC CCACGATGGG
  301 GACGGTGGGC TGGGAAGATG GCTCCCATCT CAGGGTGAGG GGCTTGGGCA GACCCTCATG
  361 CTGCACATGG CAGGTGTATC TCTGCTCCTC TCCAGAAGGT ACCACCACAG CTGCCCACTT
  421 CTGGAAGGTT CCATCCCCTG CAGGCCTGGT CTCCACAAGC TCCGTGTCCT GGGTCTGGTC
  481 CTCCCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG TAGAAGCCCA GGGCCCAGCA
  541 TCTCAGAGTG GCCTCATGGT CAGAGATGGG GTGGTGGGTC ATATGTGTCT TGGGGGGGTC
  601 CGTGCGCTGC AGCGTCTCCT TCCCGTTCTC CAGGTATCTG CGGAGCCCGT CCACGCACGT
  661 GCCCTCCAGG TAGGCTCTCT GCTGCTCCGC CACATGGGCC GCCTCCCACT TGCGCTTGGT
  721 GATCTGAGCC GCCATGTCCG CCGCGGTCCA AGAGCGCAGG TCCTCTTTCA GGGCGATGTA
  781 ATCCTTGCCG TCGTAGGCGT ACTGGTGGTA CCCGCGGAGG AAGCGCCCGT CCGACCCCAC
  841 GTCGCAGCCA AACATCATCT GGAGGGTGTG AGAACCGGCC TCGCTCTGGT TGTAGTAGCG
  901 GAGCGCGATC CGCACGTTCT CTCGGTCAGT CTGTGAGTGG GCCTTCACTT TCCCTGTCTC
  961 CTCGTCCCAA TACTCCGGGC CCCTCCTGCT CTATCCACGG CGCCGCGGCT CATCCTCTGG
 1021 GCTCGCGGCG TTCGCTGTCG ATGCACGAAC TTGCGTGTCG TCACGTAGCC CACGCGATGA
1081 GCGAGCTCCT CGCGGCCGGA CCGGACACGG CATGGTGGTA GA
//
Primer: SV40pro_F_V2 RDB14004_A6D4c.seq
>03365_14004_A6D4_SV40pro_F_V2_F10_16.ab1
    1 TGGGCCTTTT GGAGCCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
   61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
  841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
  901 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC
  961 CGCCGCCTTC TATGAAAAGG TTGGGCTTCG GGAATCGTTT TCCCGGACGC CGGGCTGGGA
 1021 ATGATCCTCC CAGCGCGGGG AATCTCATGG CTGAGTCTTC GCCCACCCCG ACCTGTTATG
1081 CAGCTTATAT GATTACTAAT AAGCATAGCC ATCACAATTT CCACAAGTAA AGCCAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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