Human MAZ cDNA is cloned into EcoRI site of pcDNA3
Alternative name | pCMV-MAZi |
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Clone info. | Human MAZ cDNA is cloned into EcoRI site of pcDNA3. |
Comment | The DNA fragment corresponds to 1..1760nt of NM_002383.4. Known differences (at least): T to C substitution at 4nt, 29 bp(GAGCCCGGGCCCCGCGCGGCCCGCGCCCC) deletion at 96 to 124nt, C to T substitution at 149nt, 15 bp(GGCGGCAGCGGCAGC) deletion at 1493 to 1507nt. In the case of a DNA fragment corresponding to 1..1738nt of D85131.1. Known differences (at least): T to C substitution at 8nt, T to CGC substitution at 14nt, insertion of G between 15 and 16nt, insertion of G between 31 and 32nt, insertion of G between 34 and 35nt, insertion of G between 53 and 54nt, T to CG substitution at 68nt, insertion of G between 74 and 75nt, insertion of G between 81 and 82nt, insertion of C between 82 and 83nt, deletion of A at 97nt, deletion of C at 99nt, insertion of 7 bp (CGGGGGC) at 123 to 124nt, insertion of C between 127 and 128nt, insertion of G between 131 and 132nt, deletion of G at 141nt, insertion of C between 144 and 145nt, insertion of G between 147 and 148nt, insertion of G between 160 and 161nt, T to G substitution at 241nt. |
Vector backbone | pcDNA3 (Plasmid) |
Size of vector backbone | 5.446 bp |
Selectable markers | Amp^r |
Gene/insert name | Human MAZ cDNA |
Depositor|Developer | Yokoyama, Kazunari | Song, Jun | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | Manuscripts for the publication shall be made through the discussion between Developer/Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).). Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。共著とする。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem. Biophys. Res. Commun., 226, 801-809 (1996).)。本リソースを使用して商業利用する場合は、寄託者と改めて契約する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB03219 | pCMV-MAZ | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCMV-MAZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03219). |
Reference section:
Tsutsui, H., Sakatsume, O., Itakura, K., Yokoyama, K.K., Members of the MAZ family: a novel cDNA clone for MAZ from human pancreatic islet cells. Biochem. Biophys. Res. Commun., 226, 801-809 (1996). PMID 8831693. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18960_B1Dkp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: inseert 5' Sequence file: RDB18960_B1Dka.seq ![]() |
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>03219_18960_B1Dk_2_CMV-Forward_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNACCCACNG CTTACNGGCT TATCGAAATT AATACGACTC 61 ACTATAGGGA GACCCAAGCT TGGTACCGAG CTCGGATCCA CTAGTAACGG CCGCCAGTGT 121 GCTGGAATTC CCGGGGTCGC CGCGGCGCTC CGCGGCCCGC AAGGCGCCCT CCTTTCCTCC 181 CTCCCGCCGG CCGGGGTGCG CGGGCGGCGG GGCGGCCCGC GGGCCATGCG TTCGGCGCGG 241 CCCAGCCCGG CCGGCCGGGG GCGGCGCCCC CGGCCCCCGC TGAGCCCTGG GGGCCCCGCT 301 GCGGCCGAGG CCATGTTCCC GGTGTTTCCT TGCACGCTGC TGGCCCCCCC CTTCCCCGTG 361 CTGGGCCTGG ACTCCCGGGG GGTGGGCGGC CTCATGAACT CCTTCCCGCC ACCTCAGGGT 421 CACGCCCAGA ACCCCCTGCA GGTCGGGGCT GANCTCCAGT CCCGCTTCTT TGCCTCCCAG 481 GGCTGCGCCC AGAGTCCATT CCAGGCCGCG CCGGCGCCCC CGCCCACGCC CCANGCCCCN 541 GCGGCCGANC CCCTCCAGGT GGANTTGCTC CCGGTGCTCG CCGCCGCCCA GGANTCCGCC 601 GCGGCTGCTG CNGCCGCTGC CGCCGCTGCT GCCNNNNTCN CNNNCNNNCC CCNNGNCCCN 661 NNNNNNNNN // |
Primer: bGH_rev3 (Pr0885) Region: insert 3' Sequence file: RDB18960_B1Dkb.seq ![]() |
>03219_18960_B1Dk_2_bGH_rev3_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NCNNNAGCAT TTAGGTGACA CTATAGAATA GGGCCCTCTA 61 GATGCATGCT CGAGCGGCCG CCAGTGTGAT GGATATCTGC AGAATTCCCC TAAGAAAACA 121 TTTCTTCCTC CTTTCCTTCT GGAGGCTCCT TGGTTGGTAG GGAAATAGGA GTTGGTGGGA 181 AAAGAGGGGG GAGAGGAGCT TGTACCAAGG GACTCTACTC CATTCTCCCC TCTCCCCCGC 241 AACCAACTTG GAGCTCACCA GGGTTGGGAG GGAAGTGGCT GAGAGCTCAC AGGCACCCCC 301 TCCGCCCCCG AGAGGGAGCC CACAGCTGTG GGAGGGGCTG CTACTGCTGC CGCTGCTGCC 361 GCTGCCGCCG CCATTGGACA AACCTCACCA GTACCTTTGT TGCAGAGCTC ACAGACATGG 421 TGAGGACCCT GGCTGTGCAC CTTCATGTGG TCCGAAATAT AAGCCGAGCT CAGCATCTTG 481 CCACACACGT GACATGGCAC TTTCTCCTCN TGTCGTACTG TGTGCGCCCG CAGCCNATCC 541 TTCNTGGCGA AAGCTGNCCT CNCATTTCTC ACATTTGAAN GGCCGTTCTG TTGANTGCNC 601 TTGNCTGANN TGANNGTTGA NGTGATNNNG CCNGGANNNN CTCTTGCCAN NNNNNNGNNN 661 CNNNNNNNNG GNNNNNNGNN NNNNNNCNNN NNNNNNN // |
Primer: SV40pro_ori_F (Pr0731) Region: NeoR Sequence file: RDB18960_B1Dkc.seq ![]() |
>03219_18960_B1Dk_2_SV40pro_ori_F_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NANAANCNNC CGGGAGCTTN NATATCCATT TTCGGATCNG 61 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT 121 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 181 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 241 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG 301 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAANGGACT 361 GGCTGCTATT GGGCGAANTG CCNGGGCANG NNCTCCTGTC NTCTCACCTT GCTCCTGCCN 421 ANAAAGTATC CNTCATGGNT GANGCNANGC NGCGGCTGNN TACNCTTGAT NCNGCTACCN 481 GCCNNTTNNA CNNNCNAGCN AANNNTCNNN TCNANNGANC NNNTANNNNN NNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Tsutsui, H., Members of the MAZ family: a novel cDNA clone for MAZ from human pancreatic islet cells. Biochem. Biophys. Res. Commun., 226, 801-809 (1996). PMID 8831693. [link to RRC of NBRP] |
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user_report | Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res., 41(7):4049-4064 (2013). PMID 23471001. [link to RRC of NBRP] |
user_report | Tsui K.H., Mechanisms by which Interleukin-6 Regulates Prostate Specific Antigen Gene Expression in Prostate LNCaP Carcinoma Cells. J. Androl. 32 (4): 383-393 (2011). PMID 21051589. [link to RRC of NBRP] |
user_report | Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711. [link to RRC of NBRP] |
user_report | Milena, M., Involvement of ubiquitous and tale transcription factors, as well as liganded RXR alpha, in the regulation of human SOX2 gene expression in the NT2/D1 embryonal carcinoma cell line. Arch. Biol. Sci., Belgrade, 62, 199-210 (2010). [link to RRC of NBRP] |
user_report | Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603. [link to RRC of NBRP] |
user_report | Frensing T., Characterization of a neuregulin-1 gene promoter: positive regulation of type I isoforms by NF-kappaB. Biochim. Biophys. Acta - Gene Regul. Mech., 1779, 139-144 (2008). PMID 18082154. [link to RRC of NBRP] |
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