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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-MAZ

Human MAZ cDNA is cloned into EcoRI site of pcDNA3

Catalog number RDB03219
Resource name pCMV-MAZ
Alternative name pCMV-MAZi
Clone info. Human MAZ cDNA is cloned into EcoRI site of pcDNA3.
Comment The DNA fragment corresponds to 1..1760nt of NM_002383.4. Known differences (at least): T to C substitution at 4nt, 29 bp(GAGCCCGGGCCCCGCGCGGCCCGCGCCCC) deletion at 96 to 124nt, C to T substitution at 149nt, 15 bp(GGCGGCAGCGGCAGC) deletion at 1493 to 1507nt.
In the case of a DNA fragment corresponding to 1..1738nt of D85131.1. Known differences (at least): T to C substitution at 8nt, T to CGC substitution at 14nt, insertion of G between 15 and 16nt, insertion of G between 31 and 32nt, insertion of G between 34 and 35nt, insertion of G between 53 and 54nt, T to CG substitution at 68nt, insertion of G between 74 and 75nt, insertion of G between 81 and 82nt, insertion of C between 82 and 83nt, deletion of A at 97nt, deletion of C at 99nt, insertion of 7 bp (CGGGGGC) at 123 to 124nt, insertion of C between 127 and 128nt, insertion of G between 131 and 132nt, deletion of G at 141nt, insertion of C between 144 and 145nt, insertion of G between 147 and 148nt, insertion of G between 160 and 161nt, T to G substitution at 241nt.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.446 bp
Selectable markers Amp^r
Gene/insert name Human MAZ cDNA
Depositor|Developer Yokoyama, Kazunari | Song, Jun |
Other clones in our bank

External Database
Human MAZ

          Reference sequence
            D85131,NM_002383.4

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Manuscripts for the publication shall be made through the discussion between Developer/Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Biochem. Biophys. Res. Commun., 226, 801-809 (1996).) Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する。共著とする。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem. Biophys. Res. Commun., 226, 801-809 (1996).)。本リソースを使用して商業利用する場合は、寄託者と改めて契約する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB03219 pCMV-MAZ DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Tsutsui, H., Members of the MAZ family: a novel cDNA clone for MAZ from human pancreatic islet cells. Biochem. Biophys. Res. Commun., 226, 801-809 (1996). PMID 8831693.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12831_A4Gp.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Foward
          Sequence file: RDB12831_A4Gpa.seq check
          >03219_12831_A4Gp_CMV-Foward_A01_01.ab1
              1 ACGAAATCCC TTCCCGTCCC CAATTGAGGC AAATGGGCGG TAGGCAGTGC CCCGGTGGGA
             61 GGTCTAAATA AGCAGAGCTT CTGAAATAAA TAGAGAACCC ACTGCTTACT GGCTTATCGA
            121 AATTAATACT ACTCACTATA GGGAGACCCA AGCTTGGTAC CGAGCTCGGA TCCACTAGTA
            181 ACGGCCGCCA GTGTGCTGGA ATTCCCGGGG TCGCCGCGGC GCTCCGCGGC CCGCAAGGCG
            241 CCCTCCTTTC CTCCCTCCCG CCGGCCGGGG TGCGCGGGCG GCGGGGCGGC CCGCGGGCCA
            301 TGCGTTCGGC GCGGCCCAGC CCGGCCGGCC GGGGGCGGCG CCCCCGGCCC CCGCTGAGCC
            361 CTGGGGGCCC CGCTGCGGCC GAGGCCATGT TCCCGGTGTT TCCTTGCACG CTGCTGGCCC
            421 CCCCCTTCCC CGTGCTGGGC CTGGACTCCC GGGGGGTGGG CGGCCTCATG AACTCCTTCC
            481 CGCCACCTCA GGGTCACGCC CAGAACCTCC TGCAGGTCGG GGCTTAAGCT CCAGTCCCGC
            541 TTCTTGCCTC CCAGGGCTGC GCCCAGAGTC CATTCCAGGC CGCGCCGGCG CCCCGCCACG
            601 CCCCAGGCCC GGGGCCGAAC CCCCCAGTGA AATTGCTCCG TTTTCCCGTC CCCAAGATTC
            661 CCCGCGGTTG CTGGGCCCTG CGCCCATGCT GCCCCCGTCT TCCGGCCCCG GCCTTCCGCG
            721 CTTTTTTGGG GAAAAAGCGG CCCTAAAGAC CTTCGGGCCC TTCCCCCCCC CCCCCCCCAG
            781 GGTGGGGGCC CCGGGCCAAG GGCGGGCCCC CCCCCTCCCC CCCCCCAATT TTGCGGGGGG
            841 GGGGGGGCCC ACGCGGTTAT AACCCCAACC TTCAAAGGGT GCCGGGGGGC CCCCGGTGCG
            901 GTTTTTCTTT TGGGAAAAAA AAAAAAAAAG AGAGAGGGGG GCCCCACTTT TTCCCCCTTT
            961 TTGTGGCCCC CAGGGAGATT TTAAAAAGGG ATTAAATTTT GTGGGGAGGA GGGAGAAAAT
           1021 CTATCCTAAC AGGAAGAAAG AGGGGGGGGC GGGGGTCCCC TCCCCGGGGG GGATTAAAAA
           1081 AAAGCACAAC AGTGGGGGTG CTCTATCATG TATCTCTCTA TGGAGCGGCC CCCCCTTCAA
           1141 TAAATGATAA CAACCTGCGG ATGTGGAGTG CGAAGTAAAG TACCAGGGTG TTGGGGGTGT
          1201 GTTCCGGCGC GCGTATC
          //
          Primer: BGH_rev
          Sequence file: RDB12831_A4Gpb.seq check
          >03219_12831_A4Gp_BGH_rev_B01_04.ab1
              1 GTTCCTATCC TTTAAGGGTG AACTATAGAA TAGGGCCCTC TAGATGCATG CTCGAGCGGC
             61 CGCCAGTGTG ATGGATATCT GCAGAATTCC CCTAAGAAAA CATTTCTTCC TCCTTTCCTT
            121 CTGGAGGCTC CTTGGTTGGT AGGGAAATAG GAGTTGGTGG GAAAAGAGGG GGGAGAGGAG
            181 CTTGTACCAA GGGACTCTAC TCCATTCTCC CCTCTCCCCC GCAACCAACT TGGAGCTCAC
            241 CAGGGTTGGG AGGGAAGTGG CTGAGAGCTC ACAGGCACCC CCTCCGCCCC CGAGAGGGAG
            301 CCCACAGCTG TGGGAGGGGC TGCTACTGCT GCCGCTGCTG CCGCTGCCGC CGCCATTGGA
            361 CAAACCTCAC CAGTACCTTT GTTGCAGAGC TCACAGACAT GGTGAGGACC CTGGCTGTGC
            421 ACCTTCATGT GGTCCGAAAT ATAAGCCGAG CTCAGCATCT TGCCACACAC GTGACATGGC
            481 ACTTTCTCCT CGTGTCGTAC TGTGTGCGCC CGCAGCCGAT CCTTCGTGGC GAAAGCTGCC
            541 TCACATTTCT CACATTTGAA GGGCCGTTCT GTTGAGTGCA CTTGTCTGAC GTGACTGTTG
            601 AGGTGATCCG GCCGGGAGAA GCTCTTGCCA CAGTGGGAGC AGTTGTAGGG CTTGTGCACA
            661 GCGCCGTCAT GTGAGCGCAC GTGGTAGCTC ATGCGGTCCT TGCGCTTGAA GCGCTGCTGG
            721 CACACCGGGC ACTGGTAGGG CTTCTCGTCC GAGTGCGACA GCTTGTGTCG GTTCAGGTGG
            781 TAGACGTCGC GGAAGGCCTT GCCACACATC TCGCAGGCAT GGTTCTTCCG GATGCGCTTC
            841 CCCGAGGCGG TCGTGGTCAC CACGCCACCG GCGGCCACTG CGGCAGCGCC GCCGCCGGCA
            901 CCCGCCTCTC CCCCTCCCCC GCCGGCTCCG CTCAGCTGGG GCACGCTCAG AGGCTCAGGG
            961 CACCATGGTC GGCATCTTCA TAGCACCCGA GGGGACCCGG GCCGGGCCTT GGCTCCGTGT
           1021 GATGGCTTCG TTGCCTCCGG AGATTGTAGC CCGTCTTGAA CTCTGCGCCA CAGAGCGCAG
           1081 ATGTAGCCCT GCTCTTGTCC TTCTCTCAGG CAGACGCGAT CGGCCCACGG CGAACGTCGA
          1141 GTTTTGGGGG GCCTACGCA
          //
          Primer: SV40pro_F
          Sequence file: RDB12831_A4Gpc.seq check
          >03219_12831_A4Gp_SV40pro_F_C01_07.ab1
              1 CCCCCAATCC TTGACTTTTC CGAAGTACTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
             61 CAAAAAGCTC CCGGGAGCTT GTAAAACCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
            121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
            661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
            781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
            841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
            901 TCGCCTTCTT GACGAGTTCT TTCTGAGCGG GACTCTGGGG TTTCGAAATG ACCGACCAAG
            961 CGACGCCCAA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCCTTCT ATGAAAGGTT
           1021 GGGGCTTCGG AATTCGGTTT TTCCGGGACG CCCGGCTGGA ATGATTCTTC CAGCGCGGGG
           1081 AATCTTCATG CCTGGAGTTC TTTCCACCAC CCCAAACTTG ATTTATTGCC AGCCTTATAT
          1141 TGTACAATTA AAGGCAATAG GCCATTCACC AAATTCACAA AATAACAGCC CACT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tsutsui, H., Members of the MAZ family: a novel cDNA clone for MAZ from human pancreatic islet cells. Biochem. Biophys. Res. Commun., 226, 801-809 (1996). PMID 8831693.
          user_report Cogoi, S., MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice. Nucleic Acids Res., 41(7):4049-4064 (2013). PMID 23471001.
          user_report Tsui K.H., Mechanisms by which Interleukin-6 Regulates Prostate Specific Antigen Gene Expression in Prostate LNCaP Carcinoma Cells. J. Androl. 32 (4): 383-393 (2011). PMID 21051589.
          user_report Membrino, A., G4-DNA Formation in the HRAS Promoter and Rational Design of Decoy Oligonucleotides for Cancer Therapy. PLoS One. 6 (9): e24421 (2011). PMID 21931711.
          user_report Milena, M., Involvement of ubiquitous and tale transcription factors, as well as liganded RXR alpha, in the regulation of human SOX2 gene expression in the NT2/D1 embryonal carcinoma cell line. Arch. Biol. Sci., Belgrade, 62, 199-210 (2010).
          user_report Cogoi S., The KRAS promoter responds to Myc-associated zinc finger and poly(ADP-ribose) polymerase 1 proteins, which recognize a critical quadruplex-forming GA-element. J. Biol. Chem., 285, 22003-22016 (2010). PMID 20457603.
          user_report Frensing T., Characterization of a neuregulin-1 gene promoter: positive regulation of type I isoforms by NF-kappaB. Biochim. Biophys. Acta - Gene Regul. Mech., 1779, 139-144 (2008). PMID 18082154.

          2022.05.18

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