Resource data sheet
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D2 (#RDB03180)

Reporter construct for mouse Dnmt1 genomic region (-220/+79)

Alternative name 300
Clone info. Reporter construct for mouse Dnmt1 genomic region spaning -220 to +79nt followed by luciferase. (+1 for transcription initiation).
Vector backbone pGL3-basic (Plasmid)
Size of vector backbone 4,818 bp
Selectable markers Amp^r
Gene/insert name Mouse DNA methyltransferase (cytosine-5) 1 Genomic DNA
Depositor|Developer Yokoyama, Kazunari | Kishikawa, Shotaro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Manuscripts for the publication shall be made through the discussion between Depositor and Recipient Investigator. Recipient investigator shall include Depositor/Developer as co-author in manuscript for the publication. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Eur. J. Biochem., 269, 2961-2970 (2002)). Recipient Investigator shall negotiate with Developer/Depositor when patent application and the commercialization for the products based upon Material, derivatives thereof their use is considered.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 使用にあたっては常に密な連絡をとり、発表等に関しても事前に合意を要する(Eur. J. Biochem., 269, 2961-2970 (2002).) 。共著とする。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。本リソースを使用して商業利用する場合は、寄託者と改めて契約する。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB03180 D2 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The D2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03180).

Reference section:

Kishikawa, S., Murata, T., Kimura, H., Shiota, K., Yokoyama, K.K., Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins. Eur. J. Biochem., 269, 2961-2970 (2002). PMID 12071960. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kishikawa, S., Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins. Eur. J. Biochem., 269, 2961-2970 (2002). PMID 12071960. [link to RRC of NBRP]