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pCMX-N/RBP-J (#RDB03021)

Expression vector of mouse RBP-J

Alternative name pCMX-N/RBP-2
Clone info. Expression vector of mouse RBP-J under the control of CMV promoter. Dominant negative form [pCMX-N/RBP-J (R218H), RDB03022] and constitutive active form (pCMX-N/VP16-RBP-J, RDB03023) are available.
Vector backbone pCMX-N (Plasmid)
Size of vector backbone 4,536 bp
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Depositor|Developer Honjo, Tasuku | Kuroda, Kazuki |
 
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Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03021 pCMX-N/RBP-J DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMX-N/RBP-J was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03021).

Reference section:

Kuroda, K., Tani, S., Tamura, K., Minoguchi, S., Kurooka, H., Honjo, T., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12301_A3Jm.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12301_A3Jma.seq check
>03021_12301_A3Jm_T7-20bp_C10_07.ab1
    1 CACAATGTCT CTAGAGGATA TCGAATTCCT GCAGCCCAAT ATGCCCTCCG GTTTTCCTCA
   61 GTCTCCACGT ACGTCCCCGA GGGCGCGTCC CAAAACCCGG ATAACCGGAG CGCTCCCCAT
  121 GGACTACTCG GAGGGCTTGT CCGCGGAGGA GCGGCCTGCG CATGCTCCAT CGGCGGGGAA
  181 GTTTGGTGAG CGGCCTCCAC CCAAACGACT CACTAGGGAA GCTATGCGAA ATTATTTAAA
  241 AGAACGAGGG GATCAAACAG TGCTCATTCT TCATGCAAAA GTTGCACAGA AGTCTTACGG
  301 AAATGAAAAA CGATTTTTTT GCCCTCCTCC TTGTGTGTAT CTTATGGGCA GTGGTTGGAA
  361 GAAAAAAAAA GAGCAAATGG AACGAGATGG ATGTTCTGAA CAAGAGTCTC AACCCTGTGC
  421 GTTTATTGGA ATAGGAAACA GTGACCAAGA AATGCAGCAG CTGAACCTCG AGGGGAAGAA
  481 CTACTGCACA GCCAAAACAT TGTACATATC TGATTCAGAC AAGAGAAAAC ATTTCATGTT
  541 GTCTGTAAAG ATGTTCTATG GCAACAGCGA TGACATTGGT GTGTTCCTCA GCAAGCGGAT
  601 AAAGGTCATC TCCAAACCCT CCAAAAAGAA GCAGTCACTG AAGAATGCTG ACTTGTGCAT
  661 TGCTTCAGGA ACGAAGGTGG CACTGTTCAA TCGCCTTCGG TCCCAGACAG TTAGTACCAG
  721 GTACCTGCAT GTAGAAGGAG GGAATTTCCA CGCCAGTTCA CAACAGTGGG GAGCATTTTA
  781 CATCCATCTC TTGGACGACG ACGAGTCGGA AGGAGAGGAG TTCACAGTTA GAGATGGCTA
  841 CATCCATTAC GGGCAGACTG TCAAGCTTGT GTGCTCAGTG ACTGGCATGG CACTCCCAAG
  901 ATTGATAATT AGGAAAGTTG ATAAGCAGAC GGCATTACTG GATGCAGACG ACCCTGTATC
  961 ACAACTCCAC AAATGTGCAT TTTACCTTAA GGATACAGAA AGAATGTACT TGTGCCCTTC
 1021 TCAGAAGATA ATCCCAATTT CAGCCACTCC ATGTCCAAAG ACAAATAAGG AAATGATAAA
 1081 CGATGGAGCT TCCTGGACAT CATAGCAAGG ACCAGCAGAA TACACGTTTC TTATTGAGGA
1141 ATGGGCCCCG GATCCCTGTG GC
//
Primer:
Sequence file: RDB12301_A3Jmb.seq check
>03021_12301_A3Jm_pAxEFR2_A01_01.ab1
    1 CGGGAAAAAA TGGCTTTATT TGGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
   61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
  121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
  181 ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTTTTCC ATAATTTTCT TGTATAGCAG
  241 TGCAGCTTTT TCCTTTGTGG TGTAAATAGC AAAGCAAGCA AGAGTTCTAT TACTAAACAC
  301 AGCATGACTC AAAAAACTTA GCAATTCTGA AGGAAAGTCC TTGGGGTCTT CTACCTTTCT
  361 CTTCTTTTTT GGAGGAGTAG AATGTTGAGA GTCAGCAGTA GCCTCATCAT CACTAGATGG
  421 CATTTCTTCT GAGCAAAACA GGTTTTCCTC ATTAAAGGCA TTCCACCACT GCTCCCATTC
  481 ATCAGTTCCA TAGGTTGGAA TCTAAAATAC ACAAACAATT AGAATCAGTA GTTTAACACA
  541 TTATACACTT AAAAATTTTA TATTTACCTT AGAGCTTTAA ATCTCTGTAG GTAGTTTGTC
  601 CAATTATGTC ACACCACAGA AGTAAGGTTC CTTCACAAAG ATCCTCTAGC TACCTAGCTA
  661 GCTGGCCAGG ATCCCGTCGA CGGATCCCCA ACTTTTTGCT GCATTCAAGT CAGTTTCAGT
  721 CCCAGCAAAA AGACGGTAGT TAGGACACCA CGGTTGCTGT GGACGATGTG ACACTGGTAG
  781 AATTTGTGCT GGCATTTGTG TAATTCCCCT CGCTGTTTGT GTTTGACTCA TTGGAGGGCA
  841 CTTGGCTGGA GTTGGCTCTG AGAATCGCTC CTGCAGCGCT GCAGTGTGGC CTCGGCCCTG
  901 GCTCTGGTGT GTAGGTGAAG GTAAGGCTGG TGGAGTAAAT GACCCCGTCA TTTCGGACCA
  961 AAGTCACTGG AACCTGGACT GGCTGGCGGA CCCATCTCCA ACCCTTCCCG GAATGCAGAA
 1021 ATGTCTGGGA CCACACAGAG CATGCTCTCT CACATCTGTA CATTGTTTCG GCTTCTACAT
 1081 CCCCAACCAC ACTCGTAATT GGAGTAAAGT TTTGTCCTGT AAGTCCAGGC ATGGCCTACG
1141 TTCCCCCGCC GCCATTCACT GAAAGACTTT TCTTACCGAA CACA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuroda, K., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785. [link to RRC of NBRP]
user_report Sakamoto K., Reduction of NOTCH1 expression pertains to maturation abnormalities of keratinocytes in squamous neoplasms. Lab. Invest., 92 (5): 688-702 (2012). PMID 22330335. [link to RRC of NBRP]
user_report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448. [link to RRC of NBRP]
user_report Lee, Y.J., Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells. Stem Cells and Cloning: Advances and Applications, 3: 165-173 (2010). PMID 24198522. [link to RRC of NBRP]
user_report Sakamoto K., Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells. J. Cell Sci., 121, 1613-1623 (2008). PMID 18430783. [link to RRC of NBRP]
user_report Sakamoto K., Distinct roles of EGF repeats for the Notch signaling system. Exp. Cell Res., 302, 281-291 (2005). PMID 15561108. [link to RRC of NBRP]