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pGST-AID (#RDB03001)

Plasmid vector to produce recombinant mouse AID protein fused with GST

Clone info. Plasmid vector to produce recombinant mouse AID protein fused with GST.
Vector backbone pGEX-4T-1 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse AID cDNA
Depositor|Developer Honjo, Tasuku | Muramatsu, Masamichi |
 

Distribution information

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Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp).
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RDB03001 pGST-AID DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGST-AID was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03001).

Reference section:

Muramatsu, M., Sankaranand, V.S., Anant, S., Sugai, M., Kinoshita, K., Davidson, N.O., Honjo, T., Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem., 274, 18470-18476 (1999). PMID 10373455. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12827_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F
Sequence file: RDB12827_A4Gia.seq check
>03001_12827_A4Gi_pGEX_F_C10_07.ab1
    1 GGCATCATCC AATCGGATCT GGTTCCGCGT GGATCCCCGG AATTCGCCAT GGACAGCCTT
   61 CTGATGAAGC AAAAGAAGTT TCTTTACCAT TTCAAAAATG TCCGCTGGGC CAAGGGACGG
  121 CATGAGACCT ACCTCTGCTA CGTGGTGAAG AGGAGAGATA GTGCCACCTC CTGCTCACTG
  181 GACTTCGGCC ACCTTCGCAA CAAGTCTGGC TGCCACGTGG AATTGTTGTT CCTACGCTAC
  241 ATCTCAGACT GGGACCTGGA CCCGGGCCGG TGTTACCGCG TCACCTGGTT CACCTCCTGG
  301 AGCCCGTGCT ATGACTGTGC CCGGCACGTG GCTGAGTTTC TGAGATGGAA CCCTAACCTC
  361 AGCCTGAGGA TTTTCACCGC GCGCCTCTAC TTCTGTGAAG ACCGCAAGGC TGAGCCTGAG
  421 GGGCTGCGGA GACTGCACCG CGCTGGGGTC CAGATCGGGA TCATGACCTT CAAAGACTAT
  481 TTTTACTGCT GGAATACATT TGTAGAAAAT CGTGAAAGAA CTTTCAAAGC CTGGGAAGGG
  541 CTACATGAAA ATTCTGTCCG GCTAACCAGA CAACTTCGGC GCATCCTTTT GCCCTTGTAC
  601 GAAGTCGATG ACTTGCGAGA TGCATTTCGT ATGTTGGGAT TTTGACTCGA GCGGCCGCAT
  661 CGTGACTGAC TGACGATCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC
  721 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC
  781 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT
  841 AGCGATAGCG GAGTGTATAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA
  901 TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT
  961 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
 1021 AGACAATAAC CCTGATAAAT GCTTCATAAT ATTGAAAAAA GGAGAGTATG AGTTATTCAC
 1081 ATTTCCGTGT CGCCCTTTAC TCCTTTTTTG CCGCATTTTG CCCGTTCTTG CTTTTTGCTC
1141 ACCCAGAAAA ACGCCTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Muramatsu, M., Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem., 274, 18470-18476 (1999). PMID 10373455. [link to RRC of NBRP]