Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGST-AID (#RDB03001)

Plasmid vector to produce recombinant mouse AID protein fused with GST

Clone info. Plasmid vector to produce recombinant mouse AID protein fused with GST.
Vector backbone pGEX-4T-1 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse AID cDNA
Depositor|Developer Honjo, Tasuku | Muramatsu, Masamichi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp).
When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。
理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03001 pGST-AID DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGST-AID was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03001).

Reference section:

Muramatsu, M., Sankaranand, V.S., Anant, S., Sugai, M., Kinoshita, K., Davidson, N.O., Honjo, T., Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem., 274, 18470-18476 (1999). PMID 10373455. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12827_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F
Sequence file: RDB12827_A4Gia.seq check
>03001_12827_A4Gi_pGEX_F_C10_07.ab1
    1 GGCATCATCC AATCGGATCT GGTTCCGCGT GGATCCCCGG AATTCGCCAT GGACAGCCTT
   61 CTGATGAAGC AAAAGAAGTT TCTTTACCAT TTCAAAAATG TCCGCTGGGC CAAGGGACGG
  121 CATGAGACCT ACCTCTGCTA CGTGGTGAAG AGGAGAGATA GTGCCACCTC CTGCTCACTG
  181 GACTTCGGCC ACCTTCGCAA CAAGTCTGGC TGCCACGTGG AATTGTTGTT CCTACGCTAC
  241 ATCTCAGACT GGGACCTGGA CCCGGGCCGG TGTTACCGCG TCACCTGGTT CACCTCCTGG
  301 AGCCCGTGCT ATGACTGTGC CCGGCACGTG GCTGAGTTTC TGAGATGGAA CCCTAACCTC
  361 AGCCTGAGGA TTTTCACCGC GCGCCTCTAC TTCTGTGAAG ACCGCAAGGC TGAGCCTGAG
  421 GGGCTGCGGA GACTGCACCG CGCTGGGGTC CAGATCGGGA TCATGACCTT CAAAGACTAT
  481 TTTTACTGCT GGAATACATT TGTAGAAAAT CGTGAAAGAA CTTTCAAAGC CTGGGAAGGG
  541 CTACATGAAA ATTCTGTCCG GCTAACCAGA CAACTTCGGC GCATCCTTTT GCCCTTGTAC
  601 GAAGTCGATG ACTTGCGAGA TGCATTTCGT ATGTTGGGAT TTTGACTCGA GCGGCCGCAT
  661 CGTGACTGAC TGACGATCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC
  721 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC
  781 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT
  841 AGCGATAGCG GAGTGTATAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA
  901 TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT
  961 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
 1021 AGACAATAAC CCTGATAAAT GCTTCATAAT ATTGAAAAAA GGAGAGTATG AGTTATTCAC
 1081 ATTTCCGTGT CGCCCTTTAC TCCTTTTTTG CCGCATTTTG CCCGTTCTTG CTTTTTGCTC
1141 ACCCAGAAAA ACGCCTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Muramatsu, M., Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem., 274, 18470-18476 (1999). PMID 10373455. [link to RRC of NBRP]