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pcHNDN (#RDB02986)

Expression vector for human necdin

Clone info. Human necdin genomic DNA containing coding region, which can be transcribed by CMV promoter.
Vector backbone pCDNA3.1 (+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Human necdin Genomic DNA
Reference of insert sequence AB007828 (DDBJ accession number)
Depositor|Developer Yoshikawa, Kazuaki |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene, 213, 65-72 (1998)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Gene, 213, 65-72 (1998))。

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RDB02986 pcHNDN DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcHNDN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02986).

Reference section:

Nakada, Y., Taniura, H., Uetsuki, T., Inazawa, J., Yoshikawa, K., The human chromosomal gene for necdin, a neuronal growth suppressor, in the Prader-Willi syndrome deletion region. Gene, 213, 65-72 (1998). PMID 9630521. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12971_A4Ja.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12971_A4Jaa.seq check
>02986_12971_A4Ja_CMV-Foward_F06_18.ab1
    1 TGGCCCATCG GTACCACTCC CGCCCCATTT GACGCAAGTG GGCGGGTAGG CGTGTACCCG
   61 CGGGAGGTCT ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT
  121 ATCGAAATTA ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGCGTTT AAACTTAAGC
  181 TTGGTACCGA GCTCGGATCC ACTAGTCCAG TGTGGTGGAA TTCAAGAGCT CCTGGACGCA
  241 GAGGCCCTGC CCTTGCCAGA CGGCGCAGAC ATGTCAGAAC AAAGTAAGGA TCTGAGCGAC
  301 CCTAACTTTG CAGCCGAGGC CCCCAACTCC GAGGTGCACA GCAGCCCTGG GGTTTCGGAG
  361 GGGGTTCCTC CGTCCGCGAC CCTGGCAGAG CCGCAGAGCC CTCCTCTAGG CCCGACGGCC
  421 GCTCCGCAGG CCGCGCCGCC TCCCCAGGCC CCGAACGACG AGGGCGACCC GAAGGCCCTG
  481 CAGCAGGCTG CGGAGGAGGG CCGCGCCCAC CAGGCCCCGA GCGCGGCCCA GCCGGGCCCG
  541 GCACCGCCAG CCCCGGCGCA GCTGGTGCAG AAGGCGCACG AGCTCATGTG GTACGTGCTG
  601 GTCAAGGACC AGAAGAAGAT GATCATCTGG TTTCCAGACA TGGTGAAAGA TGTCATCGGC
  661 AGCTACAAGA AGTGGTGCAG GAGCATCCTC CGGCGCACCA GCCTCATCCT CGCCCGGGTG
  721 TTCGGGCTGC ACCTGAGGCT AACCAGCCTG CACACCATGG AGTTTGCGCT GGTCAAAGCG
  781 CTGGAGCCCG AGGAGCTGGA CAGGGTGGCG CTGAGCAACC GCATGCCCAT GACAGGCCTC
  841 CTGCTCATGA TCCTGAGCCT CATCTACGTG AAGGGCCGCG GCGCCAGAGA GAGCGCCGTC
  901 TTGGAAACGT GCTGCGCATC CTGGGGCTGC CGGCCCTGGA AGAAGCACTC CACCTTTCGG
  961 GGACGTGCGG AAGCTCAATC ACTGAGGAGT TCGTCCCAAA TGAAATTACC TGAAGTACCA
 1021 GCGCGTCCCA TACGTGGGAG CCGCCCGATT ACGAGTTCCT TTTTGGGGCT CCCCGGGCCA
 1081 GCCGGCGAAT CACCAAGATG CCAAATTCAT GGAGGTTCCT TGGCCCAGGG TCTTTTAAGA
 1141 AGAACTCCCC AGGCCTGGGT CCCTTCTCCG GAAATACAGA AGGAAGCCTC TCTGGGAGAG
1201 GGAGAGCTCC AAGAATCCTT TCTTGGTTCG
//
Primer: BGH_rev
Sequence file: RDB12971_A4Jab.seq check
>02986_12971_A4Ja_BGH_rev_G06_21.ab1
    1 GGGGAATTCT TTTGGGGGCC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG ATATCTGCAG
   61 AATTCCCTTC CACAGCCCTG GTGAGGGTCA GAAACCATTC ATCTGCCTCC AGACTTTGCT
  121 AGTCCTCAGA GACACTGCTG CGAGGGTAGT GGGCAGTGGG ATTAGCCTCC CGCAGAGCTC
  181 TGGCCTCCTC CAGAGCTTCT CTGTATCGGG AGGGCCAGGC CTGGGGGTCT TTCTTAAAGA
  241 CCCTGGCCAG GAACTCCATG ATTTGCATCT TGGTGATTTC GCGGCTGGCC CGGGAGCCCC
  301 AAAAGAACTC GTATTCGGGC GGCTCCACGT ATGGGACGCG CTGGTACTTC AGGTAATTCA
  361 TTTGGACGAA CTCCTCAGTG ATGAGCTTCC GCACGTCCCC GAAGGTGGAG TGCTTCTTCC
  421 AGGGCCGCAG CCCCAGGATG CGCAGCACGT TCCAGACGGC GCTCTCTCTG GCGCCGCGGC
  481 CCTTCACGTA GATGAGGCTC AGGATCATGA GCAGGAGGCC TGTCATGGGC ATGCGGTTGC
  541 TCAGCGCCAC CCTGTCCAGC TCCTCGGGCT CCAGCGCTTT GACCAGCGCA AACTCCATGG
  601 TGTGCAGGCT GGTTAGCCTC AGGTGCAGCC CGAACACCCG GGCGAGGATG AGGCTGGTGC
  661 GCCGGAGGAT GCTCCTGCAC CACTTCTTGT AGCTGCCGAT GACATCTTTC ACCATGTCTG
  721 GAAACCAGAT GATCATCTTC TTCTGGTCCT TGACCAGCAC GTACCACATG AGCTCGTGCG
  781 CCTTCTGCAC CAGCTGCGCC CGGGGCTGGC GGTGCCGGGC CCGGCTGGGC CGCGCTCGGG
  841 GCCTGGTGGG CGCGGCCCTC CTCCGCAGCC TGCTGCAGGG CCTTCGGGTC GCCCTCGTCG
  901 TTCGGGGCCT GGGGAGGCGG CGCGGCCCTG CGGAGCGGCC GTCGGGCCCT AGAGGAGGGC
  961 TCTGCGGCTC TGCCAGGGTC GCGGACGGAG GAACCCCCCT CCGAAACCCA GGGCTGCTGT
 1021 GCACCTCGGA GTTGGGGGCC TCGGCTGCAA AGTTAGGGTC GCTCAAAATC CTTACTTGTC
 1081 TTGAACATGT CTTGCCGCTG TTCTGGGCAA GGGCAAGGCC CTTCTGGCGT TCAAGAGGCT
 1141 CTGATTCCAC CACAACTGGG ACTAGTGAAT CCGAAGCTCT CGGGTTACAC CAGAGCTCAT
1201 TAAAGAGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakada, Y., The human chromosomal gene for necdin, a neuronal growth suppressor, in the Prader-Willi syndrome deletion region. Gene, 213, 65-72 (1998). PMID 9630521. [link to RRC of NBRP]