Resource data sheet
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pcHNDN

Expression vector for human necdin

Catalog number RDB02986
Resource name pcHNDN
Clone info. Human necdin genomic DNA containing coding region, which can be transcribed by CMV promoter.
Vector backbone pCDNA3.1 (+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Human necdin Genomic DNA
Reference of nucleotide sequence AB007828 (DDBJ accession number)
Depositor Yoshikwa, Kazuaki |
 
Other clones in our bank human NDN (NCBI Gene 4692) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Gene, 213, 65-72 (1998)).
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RDB02986 pcHNDN DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Nakada, Y., The human chromosomal gene for necdin, a neuronal growth suppressor, in the Prader-Willi syndrome deletion region. Gene, 213, 65-72 (1998). PMID 9630521.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12971_A4Ja.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12971_A4Jaa.seq
>02986_12971_A4Ja_CMV-Foward_F06_18.ab1
    1 TGGCCCATCG GTACCACTCC CGCCCCATTT GACGCAAGTG GGCGGGTAGG CGTGTACCCG
   61 CGGGAGGTCT ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT
  121 ATCGAAATTA ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGCGTTT AAACTTAAGC
  181 TTGGTACCGA GCTCGGATCC ACTAGTCCAG TGTGGTGGAA TTCAAGAGCT CCTGGACGCA
  241 GAGGCCCTGC CCTTGCCAGA CGGCGCAGAC ATGTCAGAAC AAAGTAAGGA TCTGAGCGAC
  301 CCTAACTTTG CAGCCGAGGC CCCCAACTCC GAGGTGCACA GCAGCCCTGG GGTTTCGGAG
  361 GGGGTTCCTC CGTCCGCGAC CCTGGCAGAG CCGCAGAGCC CTCCTCTAGG CCCGACGGCC
  421 GCTCCGCAGG CCGCGCCGCC TCCCCAGGCC CCGAACGACG AGGGCGACCC GAAGGCCCTG
  481 CAGCAGGCTG CGGAGGAGGG CCGCGCCCAC CAGGCCCCGA GCGCGGCCCA GCCGGGCCCG
  541 GCACCGCCAG CCCCGGCGCA GCTGGTGCAG AAGGCGCACG AGCTCATGTG GTACGTGCTG
  601 GTCAAGGACC AGAAGAAGAT GATCATCTGG TTTCCAGACA TGGTGAAAGA TGTCATCGGC
  661 AGCTACAAGA AGTGGTGCAG GAGCATCCTC CGGCGCACCA GCCTCATCCT CGCCCGGGTG
  721 TTCGGGCTGC ACCTGAGGCT AACCAGCCTG CACACCATGG AGTTTGCGCT GGTCAAAGCG
  781 CTGGAGCCCG AGGAGCTGGA CAGGGTGGCG CTGAGCAACC GCATGCCCAT GACAGGCCTC
  841 CTGCTCATGA TCCTGAGCCT CATCTACGTG AAGGGCCGCG GCGCCAGAGA GAGCGCCGTC
  901 TTGGAAACGT GCTGCGCATC CTGGGGCTGC CGGCCCTGGA AGAAGCACTC CACCTTTCGG
  961 GGACGTGCGG AAGCTCAATC ACTGAGGAGT TCGTCCCAAA TGAAATTACC TGAAGTACCA
 1021 GCGCGTCCCA TACGTGGGAG CCGCCCGATT ACGAGTTCCT TTTTGGGGCT CCCCGGGCCA
 1081 GCCGGCGAAT CACCAAGATG CCAAATTCAT GGAGGTTCCT TGGCCCAGGG TCTTTTAAGA
 1141 AGAACTCCCC AGGCCTGGGT CCCTTCTCCG GAAATACAGA AGGAAGCCTC TCTGGGAGAG
1201 GGAGAGCTCC AAGAATCCTT TCTTGGTTCG
//
Primer: BGH_rev RDB12971_A4Jab.seq
>02986_12971_A4Ja_BGH_rev_G06_21.ab1
    1 GGGGAATTCT TTTGGGGGCC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG ATATCTGCAG
   61 AATTCCCTTC CACAGCCCTG GTGAGGGTCA GAAACCATTC ATCTGCCTCC AGACTTTGCT
  121 AGTCCTCAGA GACACTGCTG CGAGGGTAGT GGGCAGTGGG ATTAGCCTCC CGCAGAGCTC
  181 TGGCCTCCTC CAGAGCTTCT CTGTATCGGG AGGGCCAGGC CTGGGGGTCT TTCTTAAAGA
  241 CCCTGGCCAG GAACTCCATG ATTTGCATCT TGGTGATTTC GCGGCTGGCC CGGGAGCCCC
  301 AAAAGAACTC GTATTCGGGC GGCTCCACGT ATGGGACGCG CTGGTACTTC AGGTAATTCA
  361 TTTGGACGAA CTCCTCAGTG ATGAGCTTCC GCACGTCCCC GAAGGTGGAG TGCTTCTTCC
  421 AGGGCCGCAG CCCCAGGATG CGCAGCACGT TCCAGACGGC GCTCTCTCTG GCGCCGCGGC
  481 CCTTCACGTA GATGAGGCTC AGGATCATGA GCAGGAGGCC TGTCATGGGC ATGCGGTTGC
  541 TCAGCGCCAC CCTGTCCAGC TCCTCGGGCT CCAGCGCTTT GACCAGCGCA AACTCCATGG
  601 TGTGCAGGCT GGTTAGCCTC AGGTGCAGCC CGAACACCCG GGCGAGGATG AGGCTGGTGC
  661 GCCGGAGGAT GCTCCTGCAC CACTTCTTGT AGCTGCCGAT GACATCTTTC ACCATGTCTG
  721 GAAACCAGAT GATCATCTTC TTCTGGTCCT TGACCAGCAC GTACCACATG AGCTCGTGCG
  781 CCTTCTGCAC CAGCTGCGCC CGGGGCTGGC GGTGCCGGGC CCGGCTGGGC CGCGCTCGGG
  841 GCCTGGTGGG CGCGGCCCTC CTCCGCAGCC TGCTGCAGGG CCTTCGGGTC GCCCTCGTCG
  901 TTCGGGGCCT GGGGAGGCGG CGCGGCCCTG CGGAGCGGCC GTCGGGCCCT AGAGGAGGGC
  961 TCTGCGGCTC TGCCAGGGTC GCGGACGGAG GAACCCCCCT CCGAAACCCA GGGCTGCTGT
 1021 GCACCTCGGA GTTGGGGGCC TCGGCTGCAA AGTTAGGGTC GCTCAAAATC CTTACTTGTC
 1081 TTGAACATGT CTTGCCGCTG TTCTGGGCAA GGGCAAGGCC CTTCTGGCGT TCAAGAGGCT
 1141 CTGATTCCAC CACAACTGGG ACTAGTGAAT CCGAAGCTCT CGGGTTACAC CAGAGCTCAT
1201 TAAAGAGT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nakada, Y., The human chromosomal gene for necdin, a neuronal growth suppressor, in the Prader-Willi syndrome deletion region. Gene, 213, 65-72 (1998). PMID 9630521.

2018.12.10

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