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pcDNA3-p73alpha (#RDB02962)

Expression vector for human p73 alpha, based on pcDNA3

Clone info. Expression vector for human p73 alpha, based on pcDNA3. cDNA fragment 106 to 2095nt of NM_005427.3 is cloned.
Comment Known differences (at least): C to T substitution at 629nt (Thr, synonym) of NM_005427.3; C to T substitution at 1922nt (Gly, synonym) of NM_005427.3.
Vector backbone pcDNA 3.1(+) (Plasmid)
Size of vector backbone 5,400 bp
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human p73 cDNA
Depositor|Developer Shimotohno, Kunitada |
 
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RDB02962 pcDNA3-p73alpha DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-p73alpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02962).

Reference section:

Ueda, Y., Hijikata, M., Takagi, S., Chiba, T., Shimotohno, K., New p73 variants with altered C-terminal structures have varied transcriptional activities. Oncogene, 18, 4993-4998 (1999). PMID 10490834. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15723_A8A4p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15723_A8A4a.seq check
>02962_15723_A8A4_CMV-Forward_C10_07_ABI24.ab1
    1 CTTTTCAAAT CCCGCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTTGGT ACCGAGCTCG GATCCGGAAG ATGGCCCAGT
  181 CCACCGCCAC CTCCCCTGAT GGGGGCACCA CGTTTGAGCA CCTCTGGAGC TCTCTGGAAC
  241 CAGACAGCAC CTACTTCGAC CTTCCCCAGT CAAGCCGGGG GAATAATGAG GTGGTGGGCG
  301 GAACGGATTC CAGCATGGAC GTCTTCCACC TGGAGGGCAT GACTACATCT GTCATGGCCC
  361 AGTTCAATCT GCTGAGCAGC ACCATGGACC AGATGAGCAG CCGCGCGGCC TCGGCCAGCC
  421 CCTACACCCC AGAGCACGCC GCCAGCGTGC CCACCCACTC GCCCTACGCA CAACCCAGCT
  481 CCACCTTCGA CACCATGTCG CCGGCGCCTG TCATCCCCTC CAACACCGAC TACCCCGGAC
  541 CCCACCACTT TGAGGTCACT TTCCAGCAGT CCAGCACGGC CAAGTCAGCC ACCTGGACGT
  601 ACTCCCCGCT CTTGAAGAAA CTCTACTGCC AGATCGCCAA GACATGCCCC ATCCAGATCA
  661 AGGTGTCCAC CCCGCCACCC CCAGGCACTG CCATCCGGGC CATGCCTGTT TACAAGAAAG
  721 CGGAGCACGT GACCGACGTC GTGAAACGCT GCCCCAACCA CGAGCTCGGG AGGGACTTCA
  781 ACGAAGGACA GTCTGCTCCA GCCAGCCACC TCATCCGCGT GGAAGGCAAT AATCTCTCGC
  841 AGTATGTGGA TGACCCTGTC ACCGGCAGGC AGAGCGTCGT GGTGCCCTAT GAGCCACCAC
  901 AGGTGGGGGA CGGAATTCAC CACCATCCTG TACAACTTCA TGTGTAACAG CAGCTGTGTA
  961 GGGGGGCATG AACCGGGCGG GCCCATCCCT CATCATCATC ACCCTGGAGA TGCGGGATGG
 1021 GCAGGTGCTG GGCCGCCGTC CCTTTGAGGC CGCCATCTGC GCCTGTCCTG ACCGCGACGA
1081 AAGCTGAATG AAGAACCACT ACCGGGGAGT AAGCAGGATC T
//
Primer: BGH_rev2
Sequence file: RDB15723_A8A4b.seq check
>02962_15723_A8A4_BGH_rev2_D10_10_ABI24.ab1
    1 CGTGCCAGGG TCAAGAGGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
  121 AGATGCATGC TCGAGTCGAC TGGACACACA GGAAGGAGAG GGGAGGCAGC TTGGGTCTCT
  181 GGGCGGTGGC GCAGGCTGCA GCCAGGCGAG GCCCTCAGTG GATCTCGGCC TCCGTGAACT
  241 CCTCCTTGAT GGGCTGCTTG CGGGCCTTGC AGTCGGGCAG GTCGAAGCCG AAGTCCGCCC
  301 ACTCGTCAGG GCCACCGCCT GGGCCGCCGC GGTTGGGGAT GGTGATGGTG TGGCGCACGC
  361 GGAAGTGCAC GGCCTCCATG ACCCGCTGGC GCTGCAGTTC CCCTGAGCCG CCGATGGAGA
  421 TGGTGGCCGC GTTGCTAGAG CGGAGCAGCT GCTGCGCGGT GCTGTAGTCG TGGCCCTGCT
  481 TCAGGTCCTG CAGGCCCCGC CAGATGGTCA TGCGGTACTG CTCGGGGATC TTCAGGGCCC
  541 CCAGGTCCTC AATGGTCAGG TTCTGCAGGT GGTAAATGCT CTGTAACCCT TGGGAGGTGA
  601 AATACTCGAT GCAGTTTGGA CACCCCAATC CTGTTAAAAA ACTGACGAGG CTGGGGTCGG
  661 CGTGGTAGGG GGGTGGCGGA GTGCAGTGGG ACCCCGAGAC CATGGACTGG GCGCTGTGGC
  721 TGCTGCTCAT CTCGCCGTTG GCTGGCACTG CGTGGCCATG GTTGTTGAGC ATCCCGGGGC
  781 CCACGGGCCC CAGGTTGGGT GTAGCTGCCG AACTGTGCGG GGGAGGCTGG CCCACCAGCT
  841 GGTTGACGGA GGGCAGCTTG TTCATGCCCC CGTGCACCTT GTTCATGGGC GAGAGGACCG
  901 GCCCGTAGGA CGGGGGGCTG TAGGTGACTC GGCCTCTGTA GGAGCTGCTG CTGCTGCCGA
  961 TAGGAGTCCA CCAGTGGCTG CGGGCACCCA ACTCCATCAG CTCCAGGCTC TCTTTCAGCT
 1021 TTCATCAGGA TCTCAAAGTC TCCCGGCCTC GCCACCTGAA GGTAGTTACG TGTTCCTCGT
1081 CTCCATGACG TCGGCATCTC ACATCGGGTC ACCAAGGACG GGGACGGCCA GGGGGGG
//
Primer: SV40pro_F
Sequence file: RDB15723_A8A4c.seq check
>02962_15723_A8A4_SV40pro_F_E10_13_ABI24.ab1
    1 GCTTGGGGCT TGGGACTTTC CAGAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
  121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
  181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
  241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
  301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
  361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
  421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
  481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
  541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
  601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
  661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
  721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
  781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
  841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
  901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGG TTCGAAATGA CCGACCAAGC
  961 GACGCCCAAC CCTGCCATCA CGAGATTTCG ATTCCCACCG CCCGCCCTTC TATGAAGGTT
 1021 GGGGCTTTCG GAATCGTTTT TCCAGGACGT CGGCTGGGAT GATTCCCTCC AGCGCGGGGA
 1081 ATCTCAATGC TTGGAGCTCT CGTCAACCTC GACTGGTTAT GCAGCTTTAT AATTGGTTAA
1141 CA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ueda, Y., New p73 variants with altered C-terminal structures have varied transcriptional activities. Oncogene, 18, 4993-4998 (1999). PMID 10490834. [link to RRC of NBRP]