Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

crb2+ (#RDB02950)

Plasmid clone of fission yeast Crb2, SPBC342.05

Alternative name rhp9+
Clone info. Plasmid clone of fission yeast (S. pombe) Crb2, a checkpoint protein.
Vector backbone pBluescript KS(+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name S. pombe crb2 Genomic DNA
Reference of insert sequence D86478 (DDBJ accession number)
Depositor|Developer Yanagida, Mitsuhiro |
 
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Genes & Dev., 11, 3387-3400 (1997)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes & Dev., 11, 3387-3400 (1997))。

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RDB02950 crb2+ DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The crb2+ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02950).

Reference section:

Saka, Y., Esashi, F., Matsusaka, T., Mochida, S., Yanagida, M., Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1. Genes & Dev., 11, 3387-3400 (1997). PMID 9407031. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13008_A4Ka.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB13008_A4Kaa.seq check
>02950_13008_A4Ka_2_M13_-40_B10_04.ab1
    1 AAGCCCGCGA TTGTATACGA CTCACTATAG GGCGAATTGG AGCTCCACCG CGGTGGCGGC
   61 CGCTCTAGAA CTAGTGGATC CCCCGGGCTG CAGTGATCGG ACGGGAACTG GTGTTTTCGC
  121 CTAGGTATGG CCGTAGACAG CTGTTAAGTT TTAATAAAGT ATATAAACGA TAGTTATCAC
  181 TCATTAGGAA TTCGAATGTA TGTTGGCTCC ACTACGAATA AGATATGAAT GTTAGCAATA
  241 TAACCTGAAA CTATATTTGA CATTCTTTTT TTAGAAATAT CATTAAACAA GCAAAATCTA
  301 TTTTCTATGA TTGTTTCAAA AAGTCAAGAT GAAAAGTTCA AAACTCAAAT TTAAAATTAC
  361 TTCTTCAACA TTACTGATTC TAACAACATA AATCTCCTAT CGAACGTATT AATAAAAGTG
  421 GAAACATGCT GTAGTTTGTA CATCAGGTCA AACAATTATT AATAAAATTA GAAAATAAGT
  481 TTTTCTACCT TGAGCCCTTC ATTTCCATAT TAATATATAT ATATATATGA TTAGCCAAAT
  541 AGCTGGTGTT GTAACTAAAC GTTCTTAACT GCTTTCATAT AAAGTAACGG TGCAACGTGT
  601 TACTTATGCA GAGCTTACAA CAGATAAATC GAAATTTGGG TTAAGCCCTG GTTAAATTTG
  661 TAGTTCTGAC AATTGTGAGG TATTTTAGAT GTTTTCAATA TTTTGTTTGA AAGTTTAACA
  721 ATATTATGGA GGTGAATGAT ACCTCTTTGC ATAAAGGATT TGGCCTTGAC ATAAATTCTC
  781 AACGTGTATT TGGAGCACAA GCAGCAATTT CTCGTAACAA TTATTCAAAA GTGAATGCTT
  841 CAATTAATCC TTCCCCTCCT AGGAGTAATG ATAATTCTAA TAAGGAGTTT AGTTATTCTA
  901 AGGATGTGAA TAACAATGGT GCTGTTGAGG AGCTAAGTCT TACGCAGTTG TTTGAAGTAC
  961 CTAGTCAAGC TGCTTTTGCA AAACAGAGTT CCCAGGACAT TTCGGATGAT GAACTTATTC
 1021 AACATGATTC TCGTAAGTGA TATCGTCGCC CTTATTCACC CAGCAGACAC ATACAGTGTT
 1081 TAAAACGCTT ATACGATCGG TCATCAGTAT TATCGACCAT GAAAGCTGTG GACTTCTTCA
1141 GATGTATCTA AATCCTTTCC AAATTCCCTT TTCCCCCAAT
//
Primer: Reverse2
Sequence file: RDB13008_A4Kab.seq check
>02950_13008_A4Ka_2_Reverse2_E10_13.ab1
    1 AAGGTAAAAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
   61 ACCGGGCCCC CCCTCGCTAG CATACCTTGC AAACGATTCA ATATCACAAG TTTGAATCGA
  121 GACAAGTACA CCGTATTCAA ATACATTTCC TATAAGGAAG ATTAGAGGTC TGTTCTTAGA
  181 TAACTATACA AATAACATAC TGGCAAAACT AGCTTTCTCT TTATCGCTAG TAGGGACGAA
  241 GAGGTTCTGC TTAGCAAGTT CGACTTTCAG TTTCAGAAGT TCCTTTTTAC AAGCATTCCA
  301 ATCCTTTCTG TCATAAAGAT CTGCTAAATG GTTCAAGTCT AATGTACTCA TATTGATGTG
  361 TATTGAGAGT GTAATAAGTT GGAACTTTTA AGAGAGCTGT AGAAACAACA CCTCTTGCCA
  421 TGCATCTCAG AATAGTCATC AATAACGAAC ACACTATAAA GGTTTACCAG CAAAAATTAA
  481 AGCTCCTTAC GGATTTTGAC GTTGTTTTAT TAGATTCATT ATATCACTTA AGCAATTGTA
  541 ACATAGTATT CACTCACAAT AATTGCTGTA GTTGCGTTAA CTCTCTTTAT CCTTTAAACT
  601 AAAGAATATG CCGGATCTTT GGTCAGAAAG TATATTAGAA GGCAGAAAAT TGAGTATTTA
  661 TCATCTTAAA AGTACTTTAG AGAAATGTCC ATTTCTATTT GGTCAAAGTA AGAAGTCTAA
  721 GGATTTTCAA TTTGGGAGTC ATCTTTTTTT GGTAGAGGAA CAAAATGTAT TTATTTTTGG
  781 CATGGAATGC ATAGTTTACG AAAAAAACAA AGAGTTTATA GTATTTGTAT CTAAAGCAGA
  841 TTCTACCGGA TTCGGCAGCA AAGGGGTATC TTGTAACTCT CTTGCTTTTT GCTGCTTAGT
  901 GACACTTATT GACGGTCTTA GAAAACAAGG AGCCGAAAAT GTAACTTTGA CTCTGTTTGC
  961 GATTGCTCAA GGTCAATATT TATTTCCTGA GTCGGTGGAC AATGGACAAA AGCATGTGCT
 1021 GAATGATTCT GGGTTTGTTA CGCTGGGTGG GGTAAATTGT TGCAAAAACC TCAGAGTATT
 1081 TATACTGACA GTGAGCACCA ACGATTCCCG GAAAGCCAAA GATCCCACAT AACTCCTAGC
1141 CTTAATTTTA ATTTGTTTCC CTGTGACCTG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Saka, Y., Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1. Genes & Dev., 11, 3387-3400 (1997). PMID 9407031. [link to RRC of NBRP]