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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human PACE4 cDNA (#RDB02938)

Expression vector of human PACE4

Alternative name Human SPC4 cDNA
Clone info. Full length human PACE4 cDNA. Expression was confirmed by depositor as follows: immunoprecipitation and Western blotting.
Vector backbone pALTER-MAX (Plasmid)
Size of vector backbone 5,534 bp
Selectable markers Cm^r
Gene/insert name Human PACE4 cDNA
Depositor|Developer Matsuda, Yoshiko | Tsuji, Akihiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Nagahama, M. et al., FEBS Lett, 434, 155-159 (1998)) are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって文献 (Nagahama, M. et al., FEBS Lett, 434, 155-159 (1998)) の引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02938 Human PACE4 cDNA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Human PACE4 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02938).

Reference section:

Tsuji, A., Hashimoto, E., Ikoma, T., Taniguchi, T., Mori, K., Nagahama, M., Matsuda, Y., Inactivation of proprotein convertase, PACE4, by alpha1-antitrypsin Portland (alpha1-PDX), a blocker of proteolytic activation of bone morphogenetic protein during embryogenesis: evidence that PACE4 is able to form an SDS-stable acyl intermediate with alpha1-PDX. J. Biochem, 126, 591-603 (1999). PMID 10467177. [link to RRC of NBRP]
Nagahama, M., Taniguchi, T., Hashimoto, E., Imamaki, A., Mori, K., Tsuji, A., Matsuda, Y., Biosynthetic processing and quaternary interactions of proprotein convertase SPC4 (PACE4). FEBS Lett, 434, 155-159 (1998). PMID 9738469. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17895_B0A8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,intron,T7pro,insert5'
Sequence file: RDB17895_B0A8a.seq check
>02938_17895_B0A8_3_CMV-Forward_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNGCC CNNTTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT
   61 CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCACT AGAAGCTTTA TTGCGGTAGT
  121 TTATCACAGT TAAATTGCTA ACGCAGTCAG TGCTTCTGAC ACAACAGTCT CGAACTTAAG
  181 CTGCAGTGAC TCTCTTAAGG TAGCCTTGCA GAAGTTGGTC GTGAGGCACT GGGCAGGTAA
  241 GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG TCGAGACAGA
  301 GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC TGACATCCAC TTTGCCTTTC
  361 TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ACTTAATACG
  421 ACTCACTATA GGCTAGCCTC GAGAATTCAC GCGTGGTACC TCTAGAGTCG ACTGCTACCC
  481 GGGCGCCGCG CGAGCCTGTC GCCGCTATGC CTCCGCGCGC GCCGCCTGCG CCCGGGCCCC
  541 GGCCGCCGCC CCGGGCCGCC GCCGCCACCG ACACCGCCGC GGGCGCGGGG GGNGCGGGGG
  601 NCGCGGNNNN NGCCGGCGGN CCCGGGTTCC GGCCGCTCGC GCCGCGTCCC TGGCGCTGGC
661 TGCTGCTGCT GGCGCTGCNN GCCNNNNNNN NNN
//
Primer: f1ori_R3 (Pr0142)
Region: SV40poly(A)
Sequence file: RDB17895_B0A8b.seq check
>02938_17895_B0A8_3_f1ori_R3_C07_03_ABI08.ab1
    1 NNNNNNNNNN NNNNGTTGGG ANGGNGNNCG GTGCGGGCCT CTTCGCTATT ACGCCNGCCC
   61 GGATCGATCC TTATCGGATT TTACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT
  121 CCCACATCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT
  181 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC
  241 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT
  301 CTGCTCGAAG CATTAACCCT CACTAAAGGG AACTTTGCGG CCGCCCGGGT CGACTGCTAC
  361 CTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTNN
421 NNNNNNNN
//
Primer: PASE4-O_F1 (Pr0809)
Region: insert mid
Sequence file: RDB17895_B0A8c.seq check
>02938_17895_B0A8_3_PASE4-O_F1_A07_01_ABI08.ab1
    1 NNNNNNNNNN NNNNGNNNNT CTTNNTTNNG TTCTGCTGCC GCACGTGCCT CCTGGCCGGG
   61 TAAGGGTGCC TAGCTGCCCA CAGAGGGCAG GCACTCCCAT CCATCCATCC GTCCACCTTC
  121 CTCCAGACTG TCGGCCAGAG TCTGTTTCAG GAGCGGCGCC CTGCACCTGA CAGCTTTATC
  181 TCCCCAGGAG CAGCATCTCT GAGCACCCAA GCCAGGTGGG TGGTGGCTCT TAAGGAGGTG
  241 TTCCTAAAAT GGTGATATCC TCTCAAATGC TGCTTGTTGG CTCCAGTCTT CCGACAAACT
  301 AACAGGAACA AAATGAATTC TGGGAATCCA CAGCTCTGGC TTTGGAGCAG CTTCTGGGAC
  361 CATAAGTTTA CTGAATCTTC AAGACCAAAG CAGAAAAGAA AGGCGCTTGG CATCACACAT
  421 CACTCTTCTC CCCGTGCTTT TCTGCGGCTG TGTAGTAAAT CTCCCCGGCC CAGCTGGCGA
  481 ACCCTGGGCC ATCCTCACAT GTGACAAAGG GCCAGCAGTC TACCTGCTCG TTGCCTGCCA
  541 CTGAGCAGTC TGGGGACGGT TTGGTCAGAC TATAAATAAG ATAGGTTTGA GGGCATAAAA
  601 TGTATGACCA CTGGGGCCGG AGTATCTATT TCTACATAGT CAGCTACTTC TGAAACTGCA
  661 GCAGTGGCTT AGAAAGTCCA ATTCCAAAGC CAGACCAGAA GANTCTATCC CCCGCAGCGC
  721 TCTCCTTTGA GCAAGCCGAG CTCTCCTTGT TACCGTGTTC TGTCTGTGTC TTCAGGAGTC
  781 TCATGGCCTG AACGANCACC TCGANCTGNT GCAGANCCTT CTGANGANAN GCAACAGGAN
  841 GCATTCTGTG GCCAGCCAAA ANGNACCCCG ATGGCCAAGC AATTCCTCTG NACAAAATGT
  901 AAAGNCAGCC ATGCATTGTN NATCATCCAT CACTTCCNNT TTTANGNAAT TGNNTTNNAA
  961 ANANNNTTNN NCNNNNNCNN NNANNNNNNN NTTTTNNNNN NNNNANNNNC NNNNNNNNNN
//
Primer: PASE4-B_R (Pr0805)
Region: insert mid
Sequence file: RDB17895_B0A8d.seq check
>02938_17895_B0A8_3_PASE4-B_R_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCTGG CGNANGANCN TAATTTGGGG CCNGGTCAGG GTGATTTCTC
   61 TCTATGCCAT CATCAAGGAT GGTGACCACC ACGTTTTTTC CTGTGTAGCC CCTCTTCCAC
  121 GCTGCCTGGA CATTCATTTC CGACCGGCAG CGACTGTTCT TGTCGCCACA ATGCAGGTAC
  181 CACATGTTGG ACCAAATGGG GTCGTTGAAG TAAAGGGCCT GCGGGTCACT TCGCACCTGT
  241 CTCTTCACCC TTCGTTTCAC TTCCTGTTGC TGGAGCCATT TCACCTGGGG GTCCATTCTG
  301 AGGAAGGTGT GAGGGCCTCT GCTACTCAAG GTTGATCTTT TAAAGGTTTT GCTGTGATAA
  361 AAATGGTAGT AATCTTCCAG GTTTCCAATC TGGCCCAAGT TGAGGTAGCC GTGCGCCGCC
  421 GCCACGCGGT CCGCCTCGGC CGGGCCGCCC AGCACTTGCA CCGCCCAGTG GTTGGTGTAG
  481 ACGGGGCGCG GCGGGGGCGC GGAGCAGGCG GCAGGCAGCG CCAGCAGCAG CAGCCAGCGC
  541 CAGGGACGCG GCGCGAGCGG CCGGAACCCG GGCCCGCCGG CGCCCCCCGC GCCCCCCGCG
  601 CCCCCCGCGC CCGCGGCGGT GTCGGTGGCG GCGGCGGCCC GGGGCGGCGG CCGGGGCCCG
  661 GGCGCANGCG GCGCGCGCGG ANGCATAGCG GCGACAGGCT CGCGCGGCGC CCGGGTAGCA
  721 GTCGACTCTA GANGTACCAC GCGTGAATTC TCGANGCTAG CCTATAGTGA GTCGTATTAA
  781 GTACTCTAGC CTTAAGANCT GTAATTGAAC TGGGGANTGG ACACCTGTGG ANAGAAAGGN
  841 AAAGTGGATG TCAGTAAGAN CAATANGTGC CTATCANAAA CGCNAGANNC NNCTCTGTCT
901 CGANNAGCCC NGTTTCNATN NNNCNNNNTN AACNNNNNNN NNN
//
Primer: PASE4-M_F (Pr0806)
Region: insert mid
Sequence file: RDB17895_B0A8e.seq check
>02938_17895_B0A8_3_PASE4-M_F_F09_18_ABI24.ab1
    1 NNNNNNNNNN GNNNNNNNNN GGCTCCNTTT TNNNNNNGGG CATCTGGGAA TGGCGGGNNA
   61 GAGGGGGNNT ACTGCTCGTG CGATGGCTAC ACCAACAGCA TCTACACCAT CTCCGTCAGC
  121 AGCGCCACCG AGAATGGCTA CAAGCCCTGG TACCTGGAAG AGTGTGCCTC CACCCTGGCC
  181 ACCACCTACA GCAGTGGGGC CTTTTATGAG CGAAAAATCG TCACCACGGA TCTGCGTCAG
  241 CGCTGTACCG ATGGCCACAC TGGGACCTCA GTCTCTGCCC CCATGGTGGC GGGCATCATC
  301 GCCTTGGCTC TAGAAGCAAA CAGCCAGTTA ACCTGGAGGG ACGTCCAGCA CCTGCTAGTG
  361 AAGACATCCC GGCCGGCCCA CCTGAAAGCG AGCGACTGGA AAGTGAACGG CGCGGGTCAT
  421 AAAGTTAGCC ATTTCTATGG ATTTGGTTTG GTGGACGCAG AAGCTCTCGT TGTGGAGGCA
  481 AAGAAGTGGA CAGCAGTGCC ATCGCAGCAC ATGTGCGTGG CCGCCTCGGA CAAGAGACCC
  541 AGGAGCATCC CCTTAGTGCA GGTGCTGCGG ACTACGGCCC TGACCAGCGC CTGCGCGGAG
  601 CACTCGGACC AGCGGGTGGT CTACTTGGAG CACGTGGTGG TTCGCACCTC CATCTCACAC
  661 CCACGCCGAG GAGACCTCCA GATCTACCTG GTTTCTCCCT CGGGAACCAA GTCTCAACTT
  721 TTGGCAAAGA GGTTGCTGGA TCTTTCCNAT GAAGGGTTTA CAAACTGGGA ANTCATGACT
  781 GTCCACTGCT GNNGANAAAA NGCTGAANGG CAGTGGANCT TGGAAATNCN AGANCTGCCA
  841 TCCCANGNNC GCAACCCGGN NAANCAAGGG AANTTGAAAG AATGGANCNN CATACNGTAT
  901 GGCNCAGCAG ANCANCCNNA CCACNCCTTC ANNNCCCNTC ANNNCNGNNN GCNNNNGNNN
961 NNNNNNN
//
Primer: PASE4-E_F (Pr0807)
Region: insert mid
Sequence file: RDB17895_B0A8f.seq check
>02938_17895_B0A8_3_PASE4-E_F_G09_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCNCGCGGNN GCTGGAGCTC TCAGCCCCAG AGCTGGAGCC
   61 NCCCAAGGCT GCCCTGTCAC CCTCCCAGGT GGAAGTTCCT GAAGATGAGG AAGATTACAC
  121 AGCTCAATCC ACCCCAGGCT CTGCTAATAT TTTACAGACC AGTGTGTGCC ATCCGGAGTG
  181 TGGTGACAAA GGCTGTGATG GCCCCAATGC AGACCAGTGC TTGAACTGCG TCCACTTCAG
  241 CCTGGGGAGT GTCAAGACCA GCAGGAAGTG CGTGAGTGTG TGCCCCTTGG GCTACTTTGG
  301 GGACACAGCA GCAAGACGCT GTCGCCGGTG CCACAAGGGG TGTGAGACCT GCTCCAGCAG
  361 AGCTGCGACG CAGTGCCTGT CTTGCCGCCG CGGGTTCTAT CACCACCAGG AGATGAACAC
  421 CTGTGTGACC CTCTGTCCTG CAGGATTTTA TGCTGATGAA AGTCAGAAAA ATTGCCTTAA
  481 ATGCCACCCA AGCTGTAAAA AGTGCGTGGA TGAACCTGAG AAATGTACTG TCTGTAAAGA
  541 AGGATTCAGC CTTGCACGGG GCAGCTGCAT TCCTGACTGT GAGCCAGGCA CCTACTTTGA
  601 CTCAGAGCTG ATCAGATGTG GGGAATGCCA TCACACCTGC GGAACCTGCG TGGGGCCAGG
  661 CAGAGAAGAG TGCATTCACT GTGCGAAAAA CTTCCACTTC CACGACTGGA AGTGTGTGCC
  721 AGCCTGTGGT GAGGGCTTCT ACNCAGAANA GNNGCCGGGC TTGCNNCACA AAGTGTGTCG
  781 AANGTGTGAC GANAACTGCT TGANCTGTGC ANGCTCNAGC ANGAACTGTA NCANGTGTAA
  841 GNNGGGCTTC ACACAGCTGG GGNCCTNCTG CATCACCNAC CACACNNNCA GCNNCGCTNA
  901 NNANNNNNTC TNNNANANNG TGAANNNCNA NNNGNNNNNC NNNNNNNANC NNTNNNTTCA
961 NNNCNGCNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,cat pro,CmR
Sequence file: RDB17895_B0A8g.seq check
>02938_17895_B0A8_3_SV40pro_ori_F_H09_24_ABI24.ab1
    1 NNNNNNNNNN GCCGCCTCNN CCTCTGNNCT ATTCNNGAAG TAGTGAGGAG GCTTTTTTGG
   61 AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGGCT
  121 AGCGAATAAA TACCTGTGAC GGAAGATCAC TTCGCAGAAT AAATAAATCC TGGTGTCCCT
  181 GTTGATACCG GGAAGCCCTG GGCCAACTTT TGGCGAAAGT GAGACGTTGA TCGGCACGTA
  241 AGAGGTTCCA ACTTTCACCA TAATCAAATA AGATCACTAC CGGGCGTATT TTTTGAGTTA
  301 TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG ATATACCACC
  361 GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC AGTTGCTCAA
  421 TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC CGTAAAGAAA
  481 AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT GAATGCTCAT
  541 CCGGAACTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG TGTTCACCCT
  601 TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG TGAATACCAC
  661 GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA CGGTGAAAAC
  721 CTGGCCTATT TCNNTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCANC CNATCCCTNN
  781 GTGANTTTCA CCAGTTTTGA TTTAAACGTG GCCNATATGG NCAACTTCTT CGCNNNCGTT
  841 TNNANNNTNN NNNAATATTA TACNNNAGGN NANNAGGNGC TGATGCNNCN GGNNATNCNN
  901 NTTCATCANG NNNTTNNNNA TGGCTTNNNN NNCNNNNGNN NGCTNNNNGN ANNNNNNNNN
961 N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuji, A., Inactivation of proprotein convertase, PACE4, by alpha1-antitrypsin Portland (alpha1-PDX), a blocker of proteolytic activation of bone morphogenetic protein during embryogenesis: evidence that PACE4 is able to form an SDS-stable acyl intermediate with alpha1-PDX. J. Biochem, 126, 591-603 (1999). PMID 10467177. [link to RRC of NBRP]
original Nagahama, M., Biosynthetic processing and quaternary interactions of proprotein convertase SPC4 (PACE4). FEBS Lett, 434, 155-159 (1998). PMID 9738469. [link to RRC of NBRP]