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Human HLA-C*14:03 cDNA

Human HLA-C*14:03 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02890
Resource name Human HLA-C*14:03 cDNA
Alternative name HLA-Cw*1403
Clone info. Human HLA-C*14:03 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*1403 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*1403

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB02890 Human HLA-C*14:03 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02890z.seq
          Remarks, protocol and/or map (gif) RDB02890.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19619_B2Enp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: insert 5'
          Sequence file: RDB19619_B2Ena.seq check
          >02890_19619_B2En_2_CMV_Forward_F08_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
             61 NNGCAGANNT CNCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTTT CTCCCCAGAC
            181 GCCGAGATGC GGGTCATGGC GCCCCGAACC CTCATCCTGC TGCTCTCGGG AGCCCTGGCC
            241 CTGACCGAGA CCTGGGCCTG CTCCCACTCC ATGAGGTATT TCTCCACATC CGTGTCCCGG
            301 CCCGGCCGCG GGGAGCCCCA CTTCATCGCA GTGGGCTACG TGGACGACAC GCAGTTCGTG
            361 CGGTTCGACA GCGACGCCGC GAGTCCAAGA GGGGAGCCGC GGGCGCCGTG GGTGGAGCAG
            421 GAGGGGCCGG AGTATTGGGA CCGGGAGACA CAGAAGTACA AGCGCCAGGC ACAGACTGAC
            481 CGAGTGAGCC TGCGGAACCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG GTCTCACACC
            541 CTCCAGTGGA TGTTTGGCTG CGACCTGGGG CCCGACGGGC GCCTCCTCCG CGGGTATGAC
            601 CAGTCCGCCT ACGACGGCAA GGATTACATC GCCCTGAACG AGGATCTGCG CTCCTGGACC
            661 GCCGCGGACA CGGCGGCTCA GATCACCCAG CGCAAGTGGG AGGCGGCCCG TGNNGCGGAG
            721 CAGCGGANAG CCTACCTGGA GGGCACGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC
            781 GGGAANGAGA CGCTGCNGCG CGCGGAACAC CCNAAGACAC ACGTGACCCA CCATCCCGTC
            841 TCTGACCNTG ANNNCACCCT GANGTGCTGG GCCCTGGGCT TCTACCNTGC NGANNTCACN
            901 CTGANNTGGC AGTGGGATNG GGANGANNNA ACTCNNNACN NCNANNTTGN GGANNNNNNN
          961 NCNGCNNNNN NNGNNNCNTT NNNNANNNNG NNNNCTNNNG NNNNNNNNNN
          //
          Primer: bGH_rev3
          Region: bGH pA,insert3'
          Sequence file: RDB19619_B2Enb.seq check
          >02890_19619_B2En_2_bGH_rev3_G05_20_ABI24.ab1
              1 NNNNNNNNNN NNNGGNGGNN GGGCNNACNA CNGATGGCTG GCAACTAGAA GGCACAGTCG
             61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCGTC TCAGGCTTTA
            121 CAAGCGATGA GAGACTCATC AGAGCCCTGG GCACTGTTGC TGGACGCAGC CTGAGAGCAG
            181 CTCCCTCCTT TTCCACCTGA GCTCTTCCTC CTACACATCA CAACAGCCAC CACAGCTCCT
            241 AGGACAGCTA GGACAGCCAG GACAGCCAGG CCAGCAACGA TGCCCACGAT GGGGATGGTG
            301 GGCTGGGAAG ACGGCTCCCA TCTCAGGGTG AGGGGCTCCG GCAGCCCCTC GTGCTGCACA
            361 TGGCACGTGT ATCTCTGCTC TTCTCCAGAA GGCACCACCA CAGCTGCCCA CTTCTGGAAG
            421 GTTCCATCTC CTGCTGGCCT GGTCTCCACA AGCTCGGTGT CCTGAGTTTG GTCCTCCCCA
            481 TCCCACTGCC AGGTCAGTGT GATCTCCGCA GGGTAGAAGC CCAGGGCCCA GCACCTCAGG
            541 GTGGCCTCAT GGTCAGAGAC GGGATGGTGG GTCACGTGTG TCTTTGGGTG TTCCGCGCGC
            601 TGCAGCGTCT CCTTCCCGTT CTCCAGGTAT CTGCGGAGCC ACTCCACGCA CGTGCCCTCC
            661 AGGTAGGCTC TCCGCTGCTC CGCCTCACGG GCCGCCTCCC ACTTGCGCTG GGTGATCTGA
            721 GCCGCCGTGT CCGCGGCGGT CCAGGANCGC AGATCCTCGT TCANGGCGAT GTAATCCTTG
            781 CCGTCGTNNG CGGANTGGTC ATACCCGCGG ANGANGCGCC CGTCNGGCCC CANGTCGCAG
            841 CCNAACATCC ACTGGANGGT GTGAGANCCG GCCTCGCTCT GGTTGTAGTA GCCGCGCNNN
            901 TNNCGCNNNN CANTCGGNCA NTCTGTGNCN GGNGCTNGNA CTTCTGTGTN NNCCGGNNCN
            961 NNTANNNCNG CCCNNNCNNG NNNNNNCCNN NNNNNCNNNN NNNNCCNNNN TNNNNNNNNN
          1021 N
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB19619_B2Enc.seq check
          >02890_19619_B2En_2_SV40pro_ori_F_H05_23_ABI24.ab1
              1 NNNNNNNNNG NNNNNGCCGC CTCTGCCTCT GANCTNTTCN NGAAGTAGTG AGGAGGCTTT
             61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
            121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
            181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
            241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
            301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
            361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
            421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
            481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
            541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
            601 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
            661 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT
            721 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
            781 CGGCTGGGTG TGGCGGANCG CTATCANGAC ATAGCGTTGG CTANCCGTGA TATTGCTGAA
            841 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGNATCGC CGCTCCCGAN
            901 NCNCAGCGCA TCGCCTTCTA TCGCCTTCTN GANGANTTCT TCTGANCGGG NNTCTGGGGN
          961 NCGAAANGAN NGANCAAGCN ANNNCNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.06.14

          GNP_filter3_RDBDEP_html_220520.pl