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Human HLA-C*14:03 cDNA

Human HLA-C*14:03 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02890
Resource name Human HLA-C*14:03 cDNA
Alternative name HLA-Cw*1403
Clone info. Human HLA-C*14:03 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*1403 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02890 Human HLA-C*14:03 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02890z.seq from Depositor
Electronic file Map RDB02890.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13725_A5Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13725_A5Lla.seq
>02890_13725_A5Ll_T7_20bp_D07_10.ab1
    1 CGTAGCATCG TTTACTTAGC TTTTCTCCCC AGACGCCGAG ATGCGGGTCA TGGCGCCCCG
   61 AACCCTCATC CTGCTGCTCT CGGGAGCCCT GGCCCTGACC GAGACCTGGG CCTGCTCCCA
  121 CTCCATGAGG TATTTCTCCA CATCCGTGTC CCGGCCCGGC CGCGGGGAGC CCCACTTCAT
  181 CGCAGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC GACAGCGACG CCGCGAGTCC
  241 AAGAGGGGAG CCGCGGGCGC CGTGGGTGGA GCAGGAGGGG CCGGAGTATT GGGACCGGGA
  301 GACACAGAAG TACAAGCGCC AGGCACAGAC TGACCGAGTG AGCCTGCGGA ACCTGCGCGG
  361 CTACTACAAC CAGAGCGAGG CCGGGTCTCA CACCCTCCAG TGGATGTTTG GCTGCGACCT
  421 GGGGCCCGAC GGGCGCCTCC TCCGCGGGTA TGACCAGTCC GCCTACGACG GCAAGGATTA
  481 CATCGCCCTG AACGAGGATC TGCGCTCCTG GACCGCCGCG GACACGGCGG CTCAGATCAC
  541 CCAGCGCAAG TGGGAGGCGG CCCGTGAGGC GGAGCAGCGG AGAGCCTACC TGGAGGGCAC
  601 GTGCGTGGAG TGGCTCCGCA GATACCTGGA GAACGGGAAG GAGACGCTGC AGCGCGCGGA
  661 ACACCCAAAG ACACACGTGA CCCACCATCC CGTCTCTGAC CATGAGGCCA CCCTGAGGTG
  721 CTGGGCCCTG GGCTTCTACC CTGCGGAGAT CACACTGACC TGGCAGTGGG GATGGGGGAG
  781 GACCAAACTC AGGACACCGA GCTTGTGGAG ACCAGGCCAG CAGGAGATGG AACCTTCCAG
  841 AAGTGGGCAG CTGTGGTGGT GCCTTCTGGA GAAGAGCAGA GATACACGTG CCATGTGCAG
  901 CACGAGGGGC TGCCGGAGCC CCTCACCCCT GAGATGGGAG CCGTCTTTCC CAGCCCCACC
  961 ATCCCCCATC GTGGGCAATC GTTTGCTGGT CCTTGCTGTC CCTTACTGTC CTAGCCTGGT
1021 CTTAAGAAGC TGTGATGGCC TGTGTGATGT TGTTAGGGAG GGCAAC
//
Primer: BGH_rev RDB13725_A5Llb.seq
>02890_13725_A5Ll_T7_BGHrev_D04_10.ab1
    1 CTTTTTAGGG GGGCTTCTAG ACTCGAGCGG CCGCGTCTCA GGCTTTACAA GCGATGAGAG
   61 ACTCATCAGA GCCCTGGGCA CTGTTGCTGG ACGCAGCCTG AGAGCAGCTC CCTCCTTTTC
  121 CACCTGAGCT CTTCCTCCTA CACATCACAA CAGCCACCAC AGCTCCTAGG ACAGCTAGGA
  181 CAGCCAGGAC AGCCAGGCCA GCAACGATGC CCACGATGGG GATGGTGGGC TGGGAAGACG
  241 GCTCCCATCT CAGGGTGAGG GGCTCCGGCA GCCCCTCGTG CTGCACATGG CACGTGTATC
  301 TCTGCTCTTC TCCAGAAGGC ACCACCACAG CTGCCCACTT CTGGAAGGTT CCATCTCCTG
  361 CTGGCCTGGT CTCCACAAGC TCGGTGTCCT GAGTTTGGTC CTCCCCATCC CACTGCCAGG
  421 TCAGTGTGAT CTCCGCAGGG TAGAAGCCCA GGGCCCAGCA CCTCAGGGTG GCCTCATGGT
  481 CAGAGACGGG ATGGTGGGTC ACGTGTGTCT TTGGGTGTTC CGCGCGCTGC AGCGTCTCCT
  541 TCCCGTTCTC CAGGTATCTG CGGAGCCACT CCACGCACGT GCCCTCCAGG TAGGCTCTCC
  601 GCTGCTCCGC CTCACGGGCC GCCTCCCACT TGCGCTGGGT GATCTGAGCC GCCGTGTCCG
  661 CGGCGGTCCA GGAGCGCAGA TCCTCGTTCA GGGCGATGTA ATCCTTGCCG TCGTAGGCGG
  721 ACTGGTCATA CCCGCGGAGG AGGCGCCCGT CGGGCCCCAG GTCGCAGCCA AACATCCACT
  781 GGAGGGTGTG AGACCCGGCC TCGCTCTGGT TGTAGTAGCC GCGCAGGTTC CGCAGGCTCA
  841 CTCGGTCAGT CTGTGCCTGG CGCTTGTACT TCTGTGTCTC CCGGTCCCAA TACTCCGGCC
  901 CCTCCTGCTC CACCCACGGC GCCCGCGGCT CCCCTCTTGG ACTCGCGGCG TCGCTGTCGA
  961 AACCGCACGA ACTGCGTGTC GTCCACGTAG CCCACTGCGA TGAAGTGGGG CTCCCCGCGG
 1021 CGGCCGGGAC ACGGATGTGG AGAAATACCT CATGGAGTGG GAGCAGGGCC AGTCTCGTCA
 1081 GACAGGCTCC CGAAGAGCAA GCAGGAATGA AGGTCCGGGC GCCATGACCC GCAATTCTCG
1141 GGCGTCTTGG GGGGAGAAAC
//
Primer: SV40pro_F RDB13725_A5Llc.seq
>02890_13725_A5Ll_SV40pro_F_E04_13.ab1
    1 CCTCCGGCCT TTTAACCAAT CCGGAGGTAG TGAGGAGGCT TTTTTGGGAG GCCTAGGCTT
   61 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG
  121 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
  181 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
  241 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
  301 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
  361 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
  421 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
  481 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
  541 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
  601 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
  661 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
  721 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA
  781 CCGCTATCAG GACATAGCGT TGGCTACCCC GTGATATTGC TGAGAGCTTG GCGGCGAATG
  841 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGGCCT
  901 TCTATCGCCT TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA TGACCGACCA
  961 AGCGACGCCA ACCTGCATCA CGAGATTTCG AATTCCACCG GCCGCTTCTA TGGAAAGGTT
 1021 GGGGCTTCCG GATCGTTTTC GGGACGCGGC CTGGAATGGA TCTTCAGCGG CGGGGGATCT
 1081 CATGCTTGAA GTCCTTCGCC ACCCAACCTG GTATGCAGCT ATAATGTTTA CAATTAAGCC
1141 AATAGCATTC CAGAATTTGC G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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