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Human HLA-C*12:02:02 cDNA

Human HLA-C*12:02:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02889
Resource name Human HLA-C*12:02:02 cDNA
Alternative name HLA-Cw*12022
Clone info. Human HLA-C*12:02:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*12022 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*12022

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB02889 Human HLA-C*12:02:02 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02889z.seq
          Remarks, protocol and/or map (gif) RDB02889.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19003_B1Eap1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB19003_B1Eaa.seq check
          >02889_19003_B1Ea_1_CMV-Forward_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNTGGG CGGTANGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTTT CTCCCCAGAC
            181 GCCGAGATGC GGGTCATGGC GCCCCGAACC CTCATCCTGC TGCTCTCGGG AGCCCTGGCC
            241 CTGACCGAGA CCTGGGCCTG CTCCCACTCC ATGAGGTATT TCTACACCGC CGTGTCCCGG
            301 CCCGGCCGCG GAGAGCCCCG CTTCATCGCA GTGGGCTACG TGGACGACAC GCAGTTCGTG
            361 CGGTTCGACA GCGACGCCGC GAGTCCAAGA GGGGAGCCGC GGGCGCCGTG GGTGGAGCAG
            421 GAGGGGCCGG AGTATTGGGA CCGGGAGACA CAGAAGTACA AGCGCCAGGC ACAGGCTGAC
            481 CGAGTGAGCC TGCGGAACCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG GTCTCACACC
            541 CTCCAGAGGA NGTACGGCTG CGACCTGGGG CCCGACGGGC GCCTCCTCCG CGGGTATGAC
            601 CAGTCCGCCT ACGACGGCNA GGNNTACNTC GCCCTGAACG AGGANCTGCG CTCCTGNANC
          661 GCTGCNGNNN NNGCGGCTCA NATNNCCCAG CGNAAGTGGG NNNNGGNCNN NNNNNNNN
          //
          Primer: bGH_rev3
          Region: bGHpA,insert 3'
          Sequence file: RDB19003_B1Eab.seq check
          >02889_19003_B1Ea_1_bGH_rev3_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGCAAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
             61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGACTCGA GCGGCCGCGT CTCAGGCTTT
            121 ACAAGCGATG AGAGACTCAT CAGAGCCCTG GGCACTGTTG CTGGACGCAG CCTGAGAGCA
            181 GCTCCCTCCT TTTCCACCTG AGCTCTTCCT CCTACACATC ACAACAGCCA TCACAGCTCC
            241 TAGGACAGCT AGGACAGCCA GGACAGCCAG GCCAGCAACG ATGCCCACGA TGGGGATGGT
            301 GGGCTGGGAA GATGGCTCCC ATCTCAGGGT GAGGGGCTCT GGCAGCCCCT CGTGCTGCAC
            361 ATGGCACGTG TATCTCTGCT CTTCTCCAGA AGGCACCACC ACAGCTGCCC ACTTCTGGAA
            421 GGTTCCATCT CCTGCTGGCC TGGTCTCCAC AAGCTCGGTG TCCTGAGTTT GGTCCTCGCC
            481 ATCCCGCTGC CAGGTCAGTG TGATCTCCGC AGGGTAGAAG CCCAGGGCCC AGCACCTCAG
            541 GGTGGCCTCA TGGTCAGAGA CGGGATGGTG GGTCACGTGT GTCTTTGGGT GTTCCGCGCG
            601 CTGCANNNNC TCCTTCCCGT TCTCCAGGTA TCTGCGGANC CNCTCNNCGC ACGTGNCCTC
            661 CAGGTANNNT CTCCACNGCT CCGNNTCNNN GNNCNNNCNN CNNCTTNNNN NGGNNNNNNN
          721 NNANNCNNCN NNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB19003_B1Eac.seq check
          >02889_19003_B1Ea_1_SV40pro_ori_F_D01_04_ABI08.ab1
              1 NNNNNNNNNN NCNNCCTCTG CCTCTGNNCT ATTCNNGAAG TAGTGAGGAG GCTTTTTTGG
             61 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA
            121 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
            181 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC
            241 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA
            301 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC
            361 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT
            421 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
            481 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC
            541 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT
            601 TGTCNATCAG GANGNNCTGG ACGAAGANCA TCANGGGCTC GCGCCNGCCG AACTGTTCGC
          661 CAGGCTCNNN GNGCGCATGC CCGANGGCNA NGNNCTCNNC NNGNNCNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl