Resource data sheet
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Human HLA-C*12:02:02 cDNA

Human HLA-C*12:02:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02889
Resource name Human HLA-C*12:02:02 cDNA
Alternative name HLA-Cw*12022
Clone info. Human HLA-C*12:02:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*12022 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02889 Human HLA-C*12:02:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02889z.seq from Depositor
Electronic file Map RDB02889.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02889_A4Amp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB02889_A4Ama.seq
>02889_02889_A4Am_T7_20bp_A09_03.ab1
    1 CAAAGCTGGC TAGCGTTTAA CTTAAGCTTT TCTCCCCAGA CGCCGAGATG CGGGTCATGG
   61 CGCCCCGAAC CCTCATCCTG CTGCTCTCGG GAGCCCTGGC CCTGACCGAG ACCTGGGCCT
  121 GCTCCCACTC CATGAGGTAT TTCTACACCG CCGTGTCCCG GCCCGGCCGC GGAGAGCCCC
  181 GCTTCATCGC AGTGGGCTAC GTGGACGACA CGCAGTTCGT GCGGTTCGAC AGCGACGCCG
  241 CGAGTCCAAG AGGGGAGCCG CGGGCGCCGT GGGTGGAGCA GGAGGGGCCG GAGTATTGGG
  301 ACCGGGAGAC ACAGAAGTAC AAGCGCCAGG CACAGGCTGA CCGAGTGAGC CTGCGGAACC
  361 TGCGCGGCTA CTACAACCAG AGCGAGGCCG GGTCTCACAC CCTCCAGAGG ATGTACGGCT
  421 GCGACCTGGG GCCCGACGGG CGCCTCCTCC GCGGGTATGA CCAGTCCGCC TACGACGGCA
  481 AGGATTACAT CGCCCTGAAC GAGGACCTGC GCTCCTGGAC CGCTGCGGAC ACGGCGGCTC
  541 AGATCACCCA GCGCAAGTGG GAGGCGGCCC GTGAGGCGGA GCAGTGGAGA GCCTACCTGG
  601 AGGGCACGTG CGTGGAGTGG CTCCGCAGAT ACCTGGAGAA CGGGAAGGAG ACGCTGCAGC
  661 GCGCGGAACA CCCAAAGACA CACGTGACCC ACCATCCCGT CTCTGACCAT GAGGCCACCC
  721 TGAGGTGCTG GGCCCTGGGC TTCTACCCTG CGGAGATCAC ACTGACCTGG CAGCGGGATG
  781 GCGAGGACCA AACTCAGGAC ACCGAGCTTG TGGAGACCAG GCCAGCAGGA GATGGAACCT
  841 TCCAGAAGTG GGCAGCTGTG GTGGTGCCTT CTGGAGAAGA GCAGAGATAC ACGTGCCATG
  901 TGCAGCACGA GGGGCTGCCA GAGCCCCTCA CCCTGAGATG GGAGCCATCT TCCCAGCCCA
  961 CCATCCCCAT CGTGGGCATC GTTGCTGGGC CTGGCTGTCC TGGCTGTCCT AGCTGTCCTA
 1021 GGAGCTGTGA TGGCTGTTGT GATGTGTAAG AAGAAGAAGC TCAAGGTGGA AAAGGAGGGG
 1081 AGCTGCTCTT CAGCTGCGTC CAGCAACAGT TGCCTAGGCT CTGGATGAGT CTCTCATCGC
1141 TTGTAAAGCC TGGAAAACGC GGGACGCCTC TCGGAGATCT A
//
Primer: BGH_rev RDB02889_A4Amb.seq
>02889_02889_A4Am_BGHrev_B09_06.ab1
    1 CTTTTAACGG GGTCCTCTAG ACTCGAGCGG CCGCGTCTCA GGCTTTACAA GCGATGAGAG
   61 ACTCATCAGA GCCCTGGGCA CTGTTGCTGG ACGCAGCCTG AGAGCAGCTC CCTCCTTTTC
  121 CACCTGAGCT CTTCCTCCTA CACATCACAA CAGCCATCAC AGCTCCTAGG ACAGCTAGGA
  181 CAGCCAGGAC AGCCAGGCCA GCAACGATGC CCACGATGGG GATGGTGGGC TGGGAAGATG
  241 GCTCCCATCT CAGGGTGAGG GGCTCTGGCA GCCCCTCGTG CTGCACATGG CACGTGTATC
  301 TCTGCTCTTC TCCAGAAGGC ACCACCACAG CTGCCCACTT CTGGAAGGTT CCATCTCCTG
  361 CTGGCCTGGT CTCCACAAGC TCGGTGTCCT GAGTTTGGTC CTCGCCATCC CGCTGCCAGG
  421 TCAGTGTGAT CTCCGCAGGG TAGAAGCCCA GGGCCCAGCA CCTCAGGGTG GCCTCATGGT
  481 CAGAGACGGG ATGGTGGGTC ACGTGTGTCT TTGGGTGTTC CGCGCGCTGC AGCGTCTCCT
  541 TCCCGTTCTC CAGGTATCTG CGGAGCCACT CCACGCACGT GCCCTCCAGG TAGGCTCTCC
  601 ACTGCTCCGC CTCACGGGCC GCCTCCCACT TGCGCTGGGT GATCTGAGCC GCCGTGTCCG
  661 CAGCGGTCCA GGAGCGCAGG TCCTCGTTCA GGGCGATGTA ATCCTTGCCG TCGTAGGCGG
  721 ACTGGTCATA CCCGCGGAGG AGGCGCCCGT CGGGCCCCAG GTCGCAGCCG TACATCCTCT
  781 GGAGGGTGTG AGACCCGGCC TCGCTCTGGT TGTAGTAGCC GCGCAGGTTC CGCAGGCTCA
  841 CTCGGTCAGC CTGTGCCTGG CGCTTGTACT TCTGTGTCTC CCGGTCCCAA TACTCCGGCC
  901 CCTCCTGCTC CACCCACGGC GCCCGCGGCT CCCCTCTTGG ACTCGCGGCG TCGCTGTCGA
  961 ACCGCACGAA CTGCGTGTCG TCCACGTAGC CCACTGCGAT GAAGCGGGGC TCTCCGCGGC
 1021 CCGGGCCGGG ACACGGCGGT GTAGAAATAC CTCATGGAGT GGGAGCAGCC CAAGGTCTCG
 1081 GTCAGGCCAA GGCTTCCGAG AGCAGCAGAT GAGGATTCGG GGCGCATGAA CCGGCAATCT
1141 CGCGTTCTGA GAGAAAAGGC TTTAGGTTAA AACGCCTAAC CAGCCTTGTG GGGATC
//
Primer: SV40pro_F RDB02889_A4Amc.seq
>02889_02889_A4Am_SV40pro_F_C04_07.ab1
    1 ACCTGGTCCT GGGGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
 1021 CGGGATCGTT TTCCGGACGC CGGCTGGAAT GATCTCAGCG GCGGGGATCT CATGCTGGAA
 1081 GTCTCGCCCA CCCCACTGGT TATGCAGCTA TATGGTTACC ATTAAGGCAT TAGCATTCAC
1141 TAACTTCCAC CAAACTAAAA AG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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