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Human HLA-C*08:01 cDNA

Human HLA-C*08:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02888
Resource name Human HLA-C*08:01 cDNA
Alternative name HLA-Cw*0801
Clone info. Human HLA-C*08:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0801 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*0801

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB02888 Human HLA-C*08:01 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02888z.seq
          Remarks, protocol and/or map (gif) RDB02888.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19618_B2Enp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB19618_B2Ena.seq check
          >02888_19618_B2En_2_CMV_Forward_F07_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
             61 NNNTNNNNNN GCAGANCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA
            121 ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTTTCT
            181 CCCCAGACGC CGAGATGCGG GTCATGGCGC CCCGAACCCT CATCCTGCTG CTCTCGGGAG
            241 CCCTGGCCCT GACCGAGACC TGGGCCTGCT CCCACTCCAT GAGGTATTTC TACACCGCCG
            301 TGTCCCGGCC CGGCCGCGGA GAGCCCCGCT TCATCGCAGT GGGCTACGTG GACGACACGC
            361 AGTTCGTGCA GTTCGACAGC GACGCCGCGA GTCCAAGAGG GGAGCCGCGG GCGCCGTGGG
            421 TGGAGCAGGA GGGGCCGGAG TATTGGGACC GGGAGACACA GAAGTACAAG CGCCAGGCAC
            481 AGACTGACCG AGTGAGCCTG CGGAACCTGC GCGGCTACTA CAACCAGAGC GAGGCCGGGT
            541 CTCACACCCT CCAGAGGATG TATGGCTGCG ACCTGGGGCC CGACGGGCGC CTCCTCCGCG
            601 GGTATAACCA GTTCGCCTAC GACGGCAAGG NTTACATCGC CCTGAATGAG GACCTGCGCT
            661 CCTGGACCGC CGCGGACACG GCGGCTCANA TCACCCAGCG CAAGTGGGAG GCGGCCCGTA
            721 CGGCGGANCA NCTGAGAGCC TACCTGGAGG GCACGTGCGT GGAGTGGNTC CGCANATACC
            781 TGGAGAACGG GAAGAAGANG CTGCNNCGCG CGGAACACCC AAAGACACAC GTGACCCACC
            841 NTCCCGTCTC TGANCNTGAN GNCNCCCTGA NGTGCTGGNN NNTGGGCTTC TACCCTGCNG
            901 ANNTNNCACT NNNNTGGNNG CNGGNTGGCN NGGANNNAAC TCNNNNNNNN ANNTTGNGGA
          961 NNNNNNNNNN
          //
          Primer: bGH_rev3
          Region: bGH pA,insert3'
          Sequence file: RDB19618_B2Enb.seq check
          >02888_19618_B2En_2_bGH_rev3_G04_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNGGGGG NNGGNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC
             61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGACTCGA GCGGCCGCGT CTCAGGCTTT
            121 ACAAGCGATG AGAGACTCAT CAGAGCCCTG GGCACTGTTG CTGGACGCAG CCTGAGAGCA
            181 GCTCCCTCCT TTTCCACCTG AGCTCTTCCT CCTACACATC ACAACAGCCA TCACAGCTCC
            241 TAGGACAGCT AGGACAGCCA GGACAGCCAG GCCAGCAACG ATGCCCACGA TGGGGATGGT
            301 GGGCTGGGAA GATGGCCCCC ATCTCAGGGT GAGGGGCTCT GGCAGCCCCT CGTGCTGCAC
            361 ATGGCACGTG TATCTCTGCT CTTCTCCAGA AGGCACCACC ACAGCTGCCC ACTTCTGGAA
            421 GGTTCCATCT CCTGCTGGCC TGGTCTCCAC AAGCTCGGTG TCCTGAGTTT GGTCCTCGCC
            481 ATCCCGCTGC CAGGTCAGTG TGATCTCCGC AGGGTAGAAG CCCAGGGCCC AGCACCTCAG
            541 GGTGGCCTCA TGGTCAGAGA CGGGATGGTG GGTCACGTGT GTCTTTGGGT GTTCCGCGCG
            601 CTGCAGCGTC TTCTTCCCGT TCTCCAGGTA TCTGCGGAGC CACTCCACGC ACGTGCCCTC
            661 CAGGTAGGCT CTCAGCTGCT CCGCCGTACG GGCCGCCTCC CACTTGCGCT GGGTGATCTG
            721 AGCCGCCGTG TCCGCGGCGG TCCAGGANCG CANGTCCTCA TTCAGGGCGA TGTAATCCTT
            781 GCCGTCGTAN GCGAACTGGT TATACCCGCG GANGNNGCGC CCGTCNGGCC CCANGTCGCA
            841 GCCATACATC CTCTGGANGG TGTGAGANCC GGCCTCGCTC TGGTTGTAGT ANCCGCGCNN
            901 GNNNCGCNNN NCACTCNGNC AGTCTGTGCC TGGCGCTTGT ACTTCTGTGT CNNCNGGNCC
          961 CNNTANNNCN GCCCNNNNNG NNNNNCCNNN NNNNCNNNNN NNCCNNNNNN NNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB19618_B2Enc.seq check
          >02888_19618_B2En_2_SV40pro_ori_F_H07_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCTCTGCCT CTGNNCTANT CNNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
            121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
            181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
            241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
            301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
            361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
            421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
            481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
            541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
            601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
            661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
            721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NTCGACTGTG
            781 GCCGGCTGGG TGTGGCGGAN CGCTATCNNG ACATAGCGTT NGCTANCCGT GATATTGCTG
            841 AAGANCTTGG CNGCNAATGG GCTGANCGCT NCCTCNNGCT TTACGGTATC NCCGCNCCNN
            901 NNNNNCANCN NNTCNCCNTC TATCNNNNTC NNGNNNANTT CNTCTNANCN GGANTNTGGG
          961 NNNCGAAATG ANNNANNNAN CNANNNCNNN ANNNTNNNNN NNNNANNNTN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.06.14

          GNP_filter3_RDBDEP_html_220520.pl