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Human HLA-C*08:01 cDNA

Human HLA-C*08:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02888
Resource name Human HLA-C*08:01 cDNA
Alternative name HLA-Cw*0801
Clone info. Human HLA-C*08:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0801 cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02888 Human HLA-C*08:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02888z.seq
Electronic file Map RDB02888.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15310_A7H1p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB15310_A7H1c.seq check
>02888_15310_A7H1_CMV-Forward_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNTT GNNGCNNNGG GCGGTAGGCG TGTACGGTGG GNGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGCGTTTAAA CTTAAGCTTT TCTCCCCAGA
  181 CGCCGAGATG CGGGTCATGG CGCCCCGAAC CCTCATCCTG CTGCTCTCGG GAGCCCTGGC
  241 CCTGACCGAG ACCTGGGCCT GCTCCCACTC CATGAGGTAT TTCTACACCG CCGTGTCCCG
  301 GCCCGGCCGC GGAGAGCCCC GCTTCATCGC AGTGGGCTAC GTGGACGACA CGCAGTTCGT
  361 GCAGTTCGAC AGCGACGCCG CGAGTCCAAG AGGGGAGCCG CGGGCGCCGT GGGTGGAGCA
  421 GGAGGGGCCG GAGTATTGGG ACCGGGAGAC ACAGAAGTAC AAGCGCCAGG CACAGACTGA
  481 CCGAGTGAGC CTGCGGAACC TGCGCGGCTA CTACAACCAG AGCGAGGCCG GGTCTCACAC
  541 CCTCCAGAGG ATGTATGGCT GCGACCTGGG GCCCGACGGG CGCCTCCTCC GCGGGTATAA
  601 CCAGTTCGCC TACGACGGCA AGGNTTACAT CGCCCTGAAT GAGGACCTGC GCTCCTGGAC
  661 CGCCGCGGAC ACGGCGGCTC AGATCACCCA GCGCAAGTGG GANGCGGCCC GTACGGCGGA
  721 GCAGCTGANA GCCTACCTGG ANGGCACGTG CGTGGAGTGG CTCCGCAGAT ACCTGGAGAA
  781 CGGGAAGAAG ANGCTGCAGC GCGCGGAACA CCCAAAGACA CACGTGACCC ACCATCCCGN
  841 CTCTGANCAT GANGNCACCC NNNNGTGCTG GGCCCNGGGC TTCTANCCTG CGGAGANNAC
  901 ACNNANCNGN CNNCGGNNTG GCGNNGNNNN NANNNNNNNN NANCNNNCTN GNNGNNNNNC
961 ANNNCNNNNN NNNNN
//
Primer: BGH_rev2
Region: bGHpA,insert 3'
Sequence file: RDB15310_A7H1d.seq check
>02888_15310_A7H1_BGH_rev2_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGG GGGNNGGGNN NNNACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CGTCTCAGGC
  121 TTTACAAGCG ATGAGAGACT CATCAGAGCC CTGGGCACTG TTGCTGGACG CAGCCTGAGA
  181 GCAGCTCCCT CCTTTTCCAC CTGAGCTCTT CCTCCTACAC ATCACAACAG CCATCACAGC
  241 TCCTAGGACA GCTAGGACAG CCAGGACAGC CAGGCCAGCA ACGATGCCCA CGATGGGGAT
  301 GGTGGGCTGG GAAGATGGCC CCCATCTCAG GGTGAGGGGC TCTGGCAGCC CCTCGTGCTG
  361 CACATGGCAC GTGTATCTCT GCTCTTCTCC AGAAGGCACC ACCACAGCTG CCCACTTCTG
  421 GAAGGTTCCA TCTCCTGCTG GCCTGGTCTC CACAAGCTCG GTGTCCTGAG TTTGGTCCTC
  481 GCCATCCCGC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG AAGCCCAGGG CCCAGCACCT
  541 CAGGGTGGCC TCATGGTCAG AGACGGGATG GTGGGTCACG TGTGTCTTTG GGTGTTCCGC
  601 GCGCTGCAGC GTCTTCTTCC CGTTCTCCAG GTATCTGCGG AGCCACTCCA CGCACGTGCC
  661 CTCCAGGTAG GCTCTCAGCT GCTCCGCCGT ACGGGCCGCC TCCCACTTGC GCTGGGTGAT
  721 CTGAGCCGCC GTGTCCGCGG CGGTCCAGGA GCGCANGTCC TCATTCANGG CGATGTAATC
  781 CTTGCCGTCN TANGCGAACT GGTTATACCC GCGGNNGANG CGCCCGTCNG GCCCCNNGTC
  841 GCANNCATAC ATCCTCTGGN NGGTGTGAGA CCCGGCCTCN CTCTGGTNNN AGTANCCGCN
  901 CNNNTTNCNC NNNNCNCTCN GNCANNCNNN NCNNNGCNCN NGNANNTCNG NNNNNCCNNN
961 NNCNNNNNNN NCNNNNNNCN NNNNNCNNNN NNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB15310_A7H1f.seq check
>02888_15310_B0J6S_SV40pro_ori_F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNCNGCCTCT GNNCNNTTCN NGAAGTAGTG AGGANGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
  121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
  181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
  241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
  301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
  361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
  421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
  481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
  541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
  601 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
  661 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT
  721 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
  781 CGGCTGGGTG TGGCGGANCG CTATCANGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
  841 GANCTTGGCG GCGAATGGGN CTGACCGCTT CCTCGNGCTT NACGGTATCG CCGCTCCCGA
  901 TTCNCANCGC ATCNCCTTCT ATCGCNTTCT NNANGANTNC NTCNNANCGG NNNTCNGGGG
961 GTNCNAANTG ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2021.03.26

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