Resource data sheet
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Human HLA-C*08:01 cDNA

Human HLA-C*08:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02888
Resource name Human HLA-C*08:01 cDNA
Alternative name HLA-Cw*0801
Clone info. Human HLA-C*08:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0801 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02888 Human HLA-C*08:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02888z.seq from Depositor
Electronic file Map RDB02888.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02888_A2Kip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_long RDB02888_A2Kia.seq
>02888_02888_A2Ki_T7Long_G09_21.ab1
    1 CAAGCCTGGG CTAGCGTTTA ACTTAAGCTT TTCTCCCCAG ACGCCGAGAT GCGGGTCATG
   61 GCGCCCCGAA CCCTCATCCT GCTGCTCTCG GGAGCCCTGG CCCTGACCGA GACCTGGGCC
  121 TGCTCCCACT CCATGAGGTA TTTCTACACC GCCGTGTCCC GGCCCGGCCG CGGAGAGCCC
  181 CGCTTCATCG CAGTGGGCTA CGTGGACGAC ACGCAGTTCG TGCAGTTCGA CAGCGACGCC
  241 GCGAGTCCAA GAGGGGAGCC GCGGGCGCCG TGGGTGGAGC AGGAGGGGCC GGAGTATTGG
  301 GACCGGGAGA CACAGAAGTA CAAGCGCCAG GCACAGACTG ACCGAGTGAG CCTGCGGAAC
  361 CTGCGCGGCT ACTACAACCA GAGCGAGGCC GGGTCTCACA CCCTCCAGAG GATGTATGGC
  421 TGCGACCTGG GGCCCGACGG GCGCCTCCTC CGCGGGTATA ACCAGTTCGC CTACGACGGC
  481 AAGGATTACA TCGCCCTGAA TGAGGACCTG CGCTCCTGGA CCGCCGCGGA CACGGCGGCT
  541 CAGATCACCC AGCGCAAGTG GGAGGCGGCC CGTACGGCGG AGCAGCTGAG AGCCTACCTG
  601 GAGGGCACGT GCGTGGAGTG GCTCCGCAGA TACCTGGAGA ACGGGAAGAA GACGCTGCAG
  661 CGCGCGGAAC ACCCAAAGAC ACACGTGACC CACCATCCCG TCTCTGACCA TGAGGCCACC
  721 CTGAGGTGCT GGGCCCTGGG CTTCTACCCT GCGGAGATCA CACTGACCTG GCAGCGGGAT
  781 GGCGAGGACC AAACTCAGGA CACCGAGCTT GTGGAGACCA GGCCAGCAGG AGATGGAACC
  841 TTCCAGAAAG TGGCAGCTGT GGTGGTGCCT TCTGGAGAAA GAGCAGAGAT ACACGTGCCA
  901 TGTGCAGCAC GAGGGGCTGC CAGAGCCCCT CACCCTGAGA TGGGGGCCAT CTTCCCAGCC
  961 CACCATCCCC ATCGTGGCAT CGTTGCTGGG CCTGGCTGTC CTGGCTGTCC TAGCTGTCCT
 1021 AGGAGCTGTG ATGGGCTGTT GTGATGTGTA AGAGAAAGAG CTCAAGGTGG AAAACGAGGG
 1081 AGCTGCTCTC AGCTGGCGTT CAGCAACAGT GCCAGGGCTC TGAATGAAGT CTTCTCAATC
1141 GGCATTGTTA AAAAGA
//
Primer: BGH_rev RDB02888_A2Kib.seq
>02888_02888_A2Ki_BGHrev_H09_24.ab1
    1 GGTTACGGGG GACTCTAGAC TCGAGCGGCC GCGTCTCAGG CTTTACAAGC GATGAGAGAC
   61 TCATCAGAGC CCTGGGCACT GTTGCTGGAC GCAGCCTGAG AGCAGCTCCC TCCTTTTCCA
  121 CCTGAGCTCT TCCTCCTACA CATCACAACA GCCATCACAG CTCCTAGGAC AGCTAGGACA
  181 GCCAGGACAG CCAGGCCAGC AACGATGCCC ACGATGGGGA TGGTGGGCTG GGAAGATGGC
  241 CCCCATCTCA GGGTGAGGGG CTCTGGCAGC CCCTCGTGCT GCACATGGCA CGTGTATCTC
  301 TGCTCTTCTC CAGAAGGCAC CACCACAGCT GCCCACTTCT GGAAGGTTCC ATCTCCTGCT
  361 GGCCTGGTCT CCACAAGCTC GGTGTCCTGA GTTTGGTCCT CGCCATCCCG CTGCCAGGTC
  421 AGTGTGATCT CCGCAGGGTA GAAGCCCAGG GCCCAGCACC TCAGGGTGGC CTCATGGTCA
  481 GAGACGGGAT GGTGGGTCAC GTGTGTCTTT GGGTGTTCCG CGCGCTGCAG CGTCTTCTTC
  541 CCGTTCTCCA GGTATCTGCG GAGCCACTCC ACGCACGTGC CCTCCAGGTA GGCTCTCAGC
  601 TGCTCCGCCG TACGGGCCGC CTCCCACTTG CGCTGGGTGA TCTGAGCCGC CGTGTCCGCG
  661 GCGGTCCAGG AGCGCAGGTC CTCATTCAGG GCGATGTAAT CCTTGCCGTC GTAGGCGAAC
  721 TGGTTATACC CGCGGAGGAG GCGCCCGTCG GGCCCCAGGT CGCAGCCATA CATCCTCTGG
  781 AGGGTGTGAG ACCCGGCCTC GCTCTGGTTG TAGTAGCCGC GCAGGTTCCG CAGGCTCACT
  841 CGGTCAGTCT GTGCCTGGCG CTTGTACTTC TGTGTCTCCC GGTCCCAATA CTCCGGCCCC
  901 TCCTGCTCCA CCCACGGCGC CCGCGGCTCC CCTCTTGGAC TCGCGGCGTC GCTGTCGAAC
  961 TGCACGAACT GCGTGTCGTC CACGTAGCCC ACTGCGATGA AGCGGGGCTC TCCGCGGCCC
 1021 GGGCCCGGGA CACGGCGGTG TAGAAAATAC CTCATGGAGT GGAGCAGCCC AGGGTCTCGG
 1081 TCAGGACCAG GGCTCCCGAG AGCCAGCCAG GATGATCATC CGGGCGCCAA TGACCCGGCA
1141 TCTCGGCGAT CTTGGGGGGA G
//
Primer: SV40pro_F RDB02888_A2Kic.seq
>02888_02888_A2Ki_SV40pro_F_H03_24.ab1
    1 CCTGTCCTGA CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  961 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA
 1021 TCGTTTTCCG GACGCCGGCT GGATGATCTC AGCGCGGGGA TCTCATGCTG GAGTTCCTCG
 1081 CCCACCCCAC TGTTATGCAG CCTATATGCT ACCAATAAAG GCATAGCATC ACAATTCACC
1141 AATAGCATTT CGCACTGCCA ATCTATAGCT G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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