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Human HLA-C*07:02 cDNA

Human HLA-C*07:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02887
Resource name Human HLA-C*07:02 cDNA
Alternative name HLA-Cw*0702
Clone info. Human HLA-C*07:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0702 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02887 Human HLA-C*07:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02887z.seq from Depositor
Electronic file Map RDB02887.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13724_A5Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13724_A5Lla.seq
>02887_13724_A5Ll_T7_20bp_A04_01.ab1
    1 CGGGAGCAAT GCGGTTTAAA CTTAAGCTTT TCTCCCCAGA CGCCGAGATG CGGGTCATGG
   61 CGCCCCGAGC CCTCCTCCTG CTGCTCTCGG GAGGCCTGGC CCTGACCGAG ACCTGGGCCT
  121 GCTCCCACTC CATGAGGTAT TTCGACACCG CCGTGTCCCG GCCCGGCCGC GGAGAGCCCC
  181 GCTTCATCTC AGTGGGCTAC GTGGACGACA CGCAGTTCGT GCGGTTCGAC AGCGACGCCG
  241 CGAGTCCGAG AGGGGAGCCG CGGGCGCCGT GGGTGGAGCA GGAGGGGCCG GAGTATTGGG
  301 ACCGGGAGAC ACAGAAGTAT AAGCGCCAGG CACAGGCTGA CCGAGTGAGC CTGCGGAACC
  361 TGCGCGGCTA CTACAACCAG AGCGAGGACG GGTCTCACAC CCTCCAGAGG ATGTCTGGCT
  421 GCGACCTGGG GCCCGACGGG CGCCTCCTCC GCGGGTATGA CCAGTCCGCC TACGACGGCA
  481 AGGATTACAT CGCCCTGAAC GAGGACCTGC GCTCCTGGAC CGCCGCGGAC ACCGCGGCTC
  541 AGATCACCCA GCGCAAGTTG GAGGCGGCCC GTGCGGCGGA GCAGCTGAGA GCCTACCTGG
  601 AGGGCACGTG CGTGGAGTGG CTCCGCAGAT ACCTGGAGAA CGGGAAGGAG ACGCTGCAGC
  661 GCGCAGAACC CCCAAAGACA CACGTGACCC ACCACCCCCT CTCTGACCAT GAGGCCACCC
  721 TGAGGTGCTG GGCCCTGGGC TTCTACCCTG CGGAGATCAC ACTGACCTGG CAGCGGGATG
  781 GGGAGGACCA GACCCAGGAC ACCGAGCTTG TGGGAGACCA GGGCCAGCAG GAGATGGGAA
  841 CCTTTCCAGA AGTGGGGCAG CTGTGGTGGT GCCTTCTGGA CAAGAGCAGA GATACACGTG
  901 CCATATGCAG CACGAGGGGC TGCAAGAGCC CCCTCACCCT GGAGGCTGGG AGCCATCTTC
  961 CCAGCCCCAA CCATCCCCAT CATGGGGCAT CGTTGCTGGC CTGGCTGTCC TGGGTTGTCC
 1021 TAAGCTGTCC CTTGGAAGCT GTGGGTCAAC GGCTATGGAT GGTGTAGGAG AGAGCTCAAG
1081 GTGGAAAAGG AGGGGGAGCT GGCTTCTCCA GGGCCTGGCG TTGTGCAGCC A
//
Primer: BGH_rev RDB13724_A5Llb.seq
>02887_13724_A5Ll_T7_BGHrev_B04_04.ab1
    1 CGTATTCGGG GGCCTCTAGA CTCGAGCGGC CGCGTCTCAG GCTTTACAAG TGATGAGAGA
   61 CTCATCAGAG CCCTGGGCAC TGTTGCTGCA CGCAGCCTGA GAGCAGCTCC CTCCTTTTCC
  121 ACCTGAGCTC TTCCTCCTAC ACATCATAGC GGTGACCACA GCTCCAAGGA CAGCTAGGAC
  181 AACCAGGACA GCCAGGCCAG CAACGATGCC CATGATGGGG ATGGTGGGCT GGGAAGATGG
  241 CTCCCAGCTC AGGGTGAGGG GCTCTTGCAG CCCCTCGTGC TGCATATGGC ACGTGTATCT
  301 CTGCTCTTGT CCAGAAGGCA CCACCACAGC TGCCCACTTC TGGAAGGTTC CATCTCCTGC
  361 TGGCCTGGTC TCCACAAGCT CGGTGTCCTG GGTCTGGTCC TCCCCATCCC GCTGCCAGGT
  421 CAGTGTGATC TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC
  481 AGAGAGGGGG TGGTGGGTCA CGTGTGTCTT TGGGGGTTCT GCGCGCTGCA GCGTCTCCTT
  541 CCCGTTCTCC AGGTATCTGC GGAGCCACTC CACGCACGTG CCCTCCAGGT AGGCTCTCAG
  601 CTGCTCCGCC GCACGGGCCG CCTCCAACTT GCGCTGGGTG ATCTGAGCCG CGGTGTCCGC
  661 GGCGGTCCAG GAGCGCAGGT CCTCGTTCAG GGCGATGTAA TCCTTGCCGT CGTAGGCGGA
  721 CTGGTCATAC CCGCGGAGGA GGCGCCCGTC GGGCCCCAGG TCGCAGCCAG ACATCCTCTG
  781 GAGGGTGTGA GACCCGTCCT CGCTCTGGTT GTAGTAGCCG CGCAGGTTCC GCAGGCTCAC
  841 TCGGTCAGCC TGTGCCTGGC GCTTGTACTT CTGTGTCTCC CGGTCCCAAT ACTCCGGCCC
  901 CTCCTGCTCC ACCCACGGCG CCCGCGGCTC CCCTCTCGGA CTCGCGGCGT CGCTGTCGAA
  961 CCGCACGAAA CTGCGTGTCG TCCACGTAGC CCACTGAGAT GAAGCGGGGC TCTCCGCGGC
 1021 CGGGCCGGGA CACGCCGGTG TCGAAAATAC CTCATGAGTG GAGCAGGCCC CAGGTCTCGG
 1081 TCAGGACCAG GCCTCCCGGA AGAGCAGCAG AGGAAGGGCT TCGGGGGCGC CCATGAACTC
1141 GCAATTTCTC GGCGTTCCTG GGGGGAAGAA AAAAGGCCCT TTA
//
Primer: SV40pro_F RDB13724_A5Llc.seq
>02887_13724_A5Ll_SV40pro_F_C06_09.ab1
    1 CATTGTCCCG GGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
  661 ATGCCCGACG GCGAGGGATC TCGTCGTGAC CCAATGGCGA TGCCTGCTTG CCGAAATATC
  721 ATGGTGGAAA ATGGGCCGCT TTTTCTGGAT TCATCGACTG TGGCCCGAGC TGGGGTGGTG
781 GAAGACCGCC GATCCACGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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