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Human HLA-C*07:02 cDNA

Human HLA-C*07:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02887
Resource name Human HLA-C*07:02 cDNA
Alternative name HLA-Cw*0702
Clone info. Human HLA-C*07:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0702 cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02887 Human HLA-C*07:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02887z.seq
Electronic file Map RDB02887.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18718_B1Bmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB18718_B1Bma.seq check
>02887_18718__B1Bm_2_CMV-Forward_A03_03_ABI24.ab1
    1 NNNNNNNNNN NTNNNNNNNN TGGGCGGTAG GCGTGTACGG TGGGAGGNNN NNNNAAGCAG
   61 AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA
  121 CTATAGGGAG ACCCAAGCTG GCTAGCGTTT AAACTTAAGC TTTTCTCCCC AGACGCCGAG
  181 ATGCGGGTCA TGGCGCCCCG AGCCCTCCTC CTGCTGCTCT CGGGAGGCCT GGCCCTGACC
  241 GAGACCTGGG CCTGCTCCCA CTCCATGAGG TATTTCGACA CCGCCGTGTC CCGGCCCGGC
  301 CGCGGAGAGC CCCGCTTCAT CTCAGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC
  361 GACAGCGACG CCGCGAGTCC GAGAGGGGAG CCGCGGGCGC CGTGGGTGGA GCAGGAGGGG
  421 CCGGAGTATT GGGACCGGGA GACACAGAAG TACAAGCGCC AGGCACAGGC TGACCGAGTG
  481 AGCCTGCGGA ACCTGCGCGG CTACTACAAC CAGAGCGAGG ACGGGTCTCA CACCCTCCAG
  541 ANGANGTCTG GCTGCGACCT GGGGCCCGAC GGGCGCCTCC TCCGCGGGTA TGANCAGTCC
  601 GCCTACGACG GCNAGGNNTA CATCNCCCTG AACNAGGANN NGCGCTCCTG GANCGNCNCG
  661 GANNNNGCGG NTNNNATNNC CCNNNGNNNG NNGNNNNNNC NNNGNGNNNN ANCNNCNNNN
721 NNNNANNNGN NNGGNNNNNN NNNN
//
Primer: bGH_rev3
Region: bGHpA,insert 3'
Sequence file: RDB18718_B1Bmb.seq check
>02887_18718__B1Bm_2_bGH_rev3_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGG NNGGGCNNAC NANNGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCG TCTCAGGCTT
  121 TACAAGTGAT GAGAGACTCA TCAGAGCCCT GGGCACTGTT GCTGCACGCA GCCTGAGAGC
  181 AGCTCCCTCC TTTTCCACCT GAGCTCTTCC TCCTACACAT CATAGCGGTG ACCACAGCTC
  241 CAAGGACAGC TAGGACAACC AGGACAGCCA GGCCAGCAAC GATGCCCATG ATGGGGATGG
  301 TGGGCTGGGA AGATGGCTCC CAGCTCAGGG TGAGGGGCTC TTGCAGCCCC TCGTGCTGCA
  361 TATGGCACGT GTATCTCTGC TCTTGTCCAG AAGGCACCAC CACAGCTGCC CACTTCTGGA
  421 AGGTTCCATC TCCTGCTGGC CTGGTCTCCA CAAGCTCGGT GTCCTGGGTC TGGTCCTCCC
  481 CATCCCGCTG CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC CAGCACCTCN
  541 GGGTGGCCTC ATGGTCAGAG AGGGGGTGGT GGGTCACGTG TGTCTTTGGG GGNTCTGNGC
  601 GCTGCANCGT CTCCTTCCCG TTCNNNNGGT ATCTGCGGAN CCNCTCCACG CACGTGNCCT
  661 CCNGGTNNGN TCTCNNCTGC TNCNCCNCNN GGNNNGCCNN CNACNTNNNN NNGGNNGANN
721 NNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB18718_B1Bmc.seq check
>02887_18718__B1Bm_2_SV40pro_ori_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTC TGCCTCTGAG CTATTCNNGA AGTAGTGAGG
   61 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
  121 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
  181 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
  241 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
  301 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
  361 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
  421 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
  481 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
  541 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
  601 GGAAGCCGGN CTTGTCNATC NGGATGATCT GGNNNAAGAN NATCNNGGCT CNCGNCNNCC
  661 NAACTGTTCN NCNNGCTNNN GNGCGNANGN CCNANGNNNG NNNNNNTNNN GNNCCNNGNN
721 NNNGCNNGNN NNNCNANNNN NNNGNNGNNA AANNNNNNNN NTTNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2021.03.05

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