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Human HLA-C*04:01:01 cDNA

Human HLA-C*04:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02886
Resource name Human HLA-C*04:01:01 cDNA
Alternative name HLA-Cw*04011
Clone info. Human HLA-C*04:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*04011 cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02886 Human HLA-C*04:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02886z.seq
Electronic file Map RDB02886.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13723_A5Llp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB13723_A5Lla.seq check
>02886_13723_A9D2_CMV-Forward_B08_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTTGN NNCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTTTC TCCCCAGACG
  181 CCGAGATGCG GGTCATGGCG CCCCGAACCC TCATCCTGCT GCTCTCGGGA GCCCTGGCCC
  241 TGACCGAGAC CTGGGCCGGC TCCCACTCCA TGAGGTATTT CTCCACATCC GTGTCCTGGC
  301 CCGGCCGCGG GGAGCCCCGC TTCATCGCAG TGGGCTACGT GGACGACACG CAGTTCGTGC
  361 GGTTCGACAG CGACGCCGCG AGTCCAAGAG GGGAGCCGCG GGAGCCGTGG GTGGAGCAGG
  421 AGGGGCCGGA GTATTGGGAC CGGGAGACAC AGAAGTACAA GCGCCAGGCA CAGGCTGACC
  481 GAGTGAACCT GCGGAAACTG CGCGGCTACT ACAACCAGAG CGAGGACGGG TCTCACACCC
  541 TCCAGAGGAT GTTTGGCTGC GACCTGGGGC CGGACGGGCG CCTCCTCCGC GGGTATAACC
  601 AGTTCGCCTA CGACGGCAAG GATTACATCG CCCTGAACGA GGATCTGCGC TCCTGGACCG
  661 CCGCGGACAC GGCGGCTCAG ATCACCCAGC GCAAGTGGGA GGCGGCCCGT GAGGCGGANC
  721 AGCGGANAGC CTACCTGGAN GGCACGTGCG TGGAGTGGCT CCGCANATAC CTGGAGANNG
  781 GGAANGAGAN GCTGCAGCGC GCGGAACACC CANNGACACA CGTGACCCAC CATCCCGTCT
  841 CTGACCATGA NGNCNNCCTG ANGTGCTGGG CCNNGGGCTT CTACCCNGCG NANNTCACAC
901 TGNNCNNGNN NNNNNNNN
//
Primer: BGH_rev2
Region: bGH p(A), insert 3'
Sequence file: RDB13723_A5Llb.seq check
>02886_13723_A5Ll_BGHrev_D03_12.ab1
    1 CTGGTTACGG GGGGCCCTCT AGACTCGAGC GGCCGCGTCT CAGGCTTTAC AAGCGATGAG
   61 AGACTCATCA GAGCCCTGGG CACTGTTGCT GGACGCAGCC TGAGAGCAGC TCCCTCCTTT
  121 TCCACCTGAG CTCTTCCTCC TACACATCAC AACAGCCACC ATAGCTCCTA GGACAGCTAG
  181 GACAGCCAGG ACAGCCAGGC CAGCAACGAT GCCCACGATG GGGATGGTGG GCTGGGAAGA
  241 CGGCTTCCAT CTCAGGGTGA GGGGCTCCGG CAGCCCCTCG TGCTGAACAT GGCACGTGTA
  301 TCTCTGCTCT TCTCCAGAAG GCACCACCAC AGCTGCCCAC TTCTGGAAGG TTCCATCTCC
  361 TGCTGGCCTG GTCTCCACAA GCTCGGTGTC CTGAGTTTGG TCCTCCCCAT CCCACTGCCA
  421 GGTCAGTGTG ATCTCCGCAG GGTAGAAGCC CAGGGCCCAG CACCTCAGGG TGGCCTCATG
  481 GTCAGAGACG GGATGGTGGG TCACGTGTGT CTTTGGGTGT TCCGCGCGCT GCAGCGTCTC
  541 CTTCCCGTTC TCCAGGTATC TGCGGAGCCA CTCCACGCAC GTGCCCTCCA GGTAGGCTCT
  601 CCGCTGCTCC GCCTCACGGG CCGCCTCCCA CTTGCGCTGG GTGATCTGAG CCGCCGTGTC
  661 CGCGGCGGTC CAGGAGCGCA GATCCTCGTT CAGGGCGATG TAATCCTTGC CGTCGTAGGC
  721 GAACTGGTTA TACCCGCGGA GGAGGCGCCC GTCCGGCCCC AGGTCGCAGC CAAACATCCT
  781 CTGGAGGGTG TGAGACCCGT CCTCGCTCTG GTTGTAGTAG CCGCGCAGTT TCCGCAGGTT
  841 CACTCGGTCA GCCTGTGCCT GGCGCTTGTA CTTCTGTGTC TCCCGGGTCC CAATACTCCG
  901 GCCCCTCCTG CTCCACCCAC GGCTCCCGCG GCTCCCCTCT TGGACTCGCG GCGTCGCTGT
  961 CGAACCCGCA CGAACTGCGT GTCGTCCACG TAGCCCACTG CGATGAAGCG GGGCTCCCCG
 1021 CGGCCGGGCC AGGACACGGA TGTGGAAGAA ATACCCTCAT GGGAGTGGGA GCCGGGCCCA
 1081 GGTCTCGGTC AGGACAGGCC TCCCGAGGAG CAGCAAGGAT GAGGGTCGGG GCGCCCAATG
1141 AACCCGCAAT TCTTCGGGCG TCTCTGGGGG GGGAGAAAAA AGGGCC
//
Primer: SV40pro_F
Region: NeoR
Sequence file: RDB13723_A5Llc.seq check
>02886_13723_A5Ll_SV40pro_F_H05_23.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNGNNGCT TTTTTGGNNG CCTANGCTTT TGCAAAAAGC
   61 TCCCGGGNNC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC
  121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  601 AAGAGCATCA NGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG
  661 ACGGCGANGN TCTCGTCGTG ANCCATGGCG ATGCCTGCTT GCCGAATATC ATGGNGGAAA
  721 ATGNNCNGCT TTTCTGGANT CNTCGACTGN GGNCGGCTGG GTGTGGCGGA NCGCTATCNN
781 NACNTANCGT NGNCTANCCN NGANNNTGNT GNAGANCNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.
user report Banerjee, P.P., KIR2DL4-HLAG interaction at human NK cell-oligodendrocyte interfaces regulates IFN-gamma-mediated effects. Mol. Immunol. pii: S0161-5890 (18) 30835-6 (2018). PMID 30482463.
user report Ichise, H., NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs. Stem Cell Reports 9 (3): 853-867 (2017). PMID 28867344.

2020.10.02

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