Resource data sheet
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Human HLA-C*03:04:01 cDNA

Human HLA-C*03:04:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02885
Resource name Human HLA-C*03:04:01 cDNA
Alternative name HLA-Cw*03041
Clone info. Human HLA-C*03:04:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*03041 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02885 Human HLA-C*03:04:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02885z.seq from Depositor
Electronic file Map RDB02885.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02885_A4Cjp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB02885_A4Cja.seq
>02885_02885_A4Cj_T7_20bp_C02_08.ab1
    1 CGTTGACAGG CCGTTTAACT TAAGCTTTTC TCCCCAGACG CCGAGATGCG GGTCATGGCG
   61 CCCCGAACCC TCATCCTGCT GCTCTCGGGA GCCCTGGCCC TGACCGAGAC CTGGGCCGGC
  121 TCCCACTCCA TGAGGTATTT CTACACCGCT GTGTCCCGGC CCGGCCGCGG GGAGCCCCAC
  181 TTCATCGCAG TGGGCTACGT GGACGACACG CAGTTCGTGC GGTTCGACAG CGACGCCGCG
  241 AGTCCGAGAG GGGAGCCGCG GGCGCCGTGG GTGGAGCAGG AGGGGCCGGA GTATTGGGAC
  301 CGGGAGACAC AGAAGTACAA GCGCCAGGCA CAGACTGACC GAGTGAGCCT GCGGAACCTG
  361 CGCGGCTACT ACAACCAGAG CGAGGCCGGG TCTCACATCA TCCAGAGGAT GTATGGCTGC
  421 GACGTGGGGC CCGACGGGCG CCTCCTCCGC GGGTATGACC AGTACGCCTA CGACGGCAAG
  481 GATTACATCG CCCTGAACGA GGATCTGCGC TCCTGGACCG CCGCGGACAC GGCGGCTCAG
  541 ATCACCCAGC GCAAGTGGGA GGCGGCCCGT GAGGCGGAGC AGCTGAGAGC CTACCTGGAG
  601 GGCCTGTGCG TGGAGTGGCT CCGCAGATAC CTGAAGAATG GGAAGGAGAC GCTGCAGCGC
  661 GCGGAACACC CAAAGACACA CGTGACCCAC CATCCCGTCT CTGACCATGA GGCCACCCTG
  721 AGGTGCTGGG CCCTGGGCTT CTACCCTGCG GAGATCACAC TGACCTGGCA GTGGGATGGG
  781 GAGGACCAAA CTCAGGACAC TGAGCTTGTG GAGACCAGGC CAGCAGGAGA TGGAACCTTC
  841 CAGAAGTGGG CAGCTGTGGT GGTGCCTTCT GGAGAAGAGC AGAGATACAC GTGCCATGTG
  901 CAGCACGAGG GGGCTGCCGG AGCCCCTCAC CCTGAGATGG GAGCCGTCTT CCCAGCCCAC
  961 CATCCCCCAT CGTGGGCATC GTTGCTGGCC TGGCTGTCCT GGCCTGTCCT AGCTGTCCTA
 1021 GGAGCTGTGG TGGGCTGTTG TGATGTGTAG GGAGGGAAGA GCTCAGGTGG AAAAGGGAGG
 1081 GAGCTGCTCT CAGCTGCGTC CAGCCAACAG TGCCCAGGCT CTGAATGAGT CTCTCATCGC
1141 TTGTAAGCCC TGGAGAACGG CCGGCGCGCC TCTCCGAG
//
Primer: BGH_rev RDB02885_A4Cjb.seq
>02885_02885_A4Cj_BGHrev_D02_11.ab1
    1 CTTTATTCGG GGGCCCTCTA GACTCGAGGC GGCCGCGTCT CAGGCTTTAC AAGCGATGAG
   61 AGACTCATCA GAGCCCTGGG CACTGTTGCT GGACGCAGCC TGAGAGCAGC TCCCTCCTTT
  121 TCCACCTGAG CTCTTCCTCC TACACATCAC AACAGCCACC ACAGCTCCTA GGACAGCTAG
  181 GACAGCCAGG ACAGCCAGGC CAGCAACGAT GCCCACGATG GGGATGGTGG GCTGGGAAGA
  241 CGGCTCCCAT CTCAGGGTGA GGGGCTCCGG CAGCCCCTCG TGCTGCACAT GGCACGTGTA
  301 TCTCTGCTCT TCTCCAGAAG GCACCACCAC AGCTGCCCAC TTCTGGAAGG TTCCATCTCC
  361 TGCTGGCCTG GTCTCCACAA GCTCAGTGTC CTGAGTTTGG TCCTCCCCAT CCCACTGCCA
  421 GGTCAGTGTG ATCTCCGCAG GGTAGAAGCC CAGGGCCCAG CACCTCAGGG TGGCCTCATG
  481 GTCAGAGACG GGATGGTGGG TCACGTGTGT CTTTGGGTGT TCCGCGCGCT GCAGCGTCTC
  541 CTTCCCATTC TTCAGGTATC TGCGGAGCCA CTCCACGCAC AGGCCCTCCA GGTAGGCTCT
  601 CAGCTGCTCC GCCTCACGGG CCGCCTCCCA CTTGCGCTGG GTGATCTGAG CCGCCGTGTC
  661 CGCGGCGGTC CAGGAGCGCA GATCCTCGTT CAGGGCGATG TAATCCTTGC CGTCGTAGGC
  721 GTACTGGTCA TACCCGCGGA GGAGGCGCCC GTCGGGCCCC ACGTCGCAGC CATACATCCT
  781 CTGGATGATG TGAGACCCGG CCTCGCTCTG GTTGTAGTAG CCGCGCAGGT TCCGCAGGCT
  841 CACTCGGTCA GTCTGTGCCT GGCGCTTGTA CTTCTGTGTC TCCCGGTCCC AATACTCCGG
  901 CCCCTCCTGC TCCACCCACG GCGCCCGCGG CTCCCCTCTC GGACTCGCGG CGTCGCTGTC
  961 GAAACCGCAC GAACTGCGTG TCGTCCACGT AGCCCACTGC GATGAAGTGG GGGCTCCCCG
 1021 CGGCCGGGGC CGGGACACAG CGGTGTAGAA ATACCCTCAT GGAGTGGGAG CCGGCCCAGT
 1081 CCTCGGTTCA GGGCCCAGGG CTCCCCGAAG AAGCCAGCAG GAATGGAGGG TTCGGGGCGC
 1141 AATGACCGCA TCTCGGCGTC TGGGGGAAGA AAAAGGCTTA AGGCTTAAAA ACGGCCTATG
1201 A
//
Primer: SV40pro_F RDB02885_A4Cjc.seq
>02885_02885_A4Cj_SV40pro_F_G03_21.ab1
    1 CCCTGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  961 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT TCTATGAAAG GTTGGGCTTC
 1021 GGGAATCGTT TTCCGGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG
 1081 AGTCTCGCCC ACCCCAAACT TTGTTAATGG CAGCCTTATA TGTACAAATA AAGCATAGCA
1141 TCACCAAACT TTCCCCAAAT TAAAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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