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Human HLA-C*03:04:01 cDNA

Human HLA-C*03:04:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02885
Resource name Human HLA-C*03:04:01 cDNA
Alternative name HLA-Cw*03041
Clone info. Human HLA-C*03:04:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*03041 cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02885 Human HLA-C*03:04:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02885z.seq
Electronic file Map RDB02885.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17870_A9L2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert5'
Sequence file: RDB17870_A9L2a.seq check
>02885_17870_A9L2_3_CMV-Forward_C06_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNN GCNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTTTCT CCCCAGACGC
  181 CGAGATGCGG GTCATGGCGC CCCGAACCCT CATCCTGCTG CTCTCGGGAG CCCTGGCCCT
  241 GACCGAGACC TGGGCCGGCT CCCACTCCAT GAGGTATTTC TACACCGCTG TGTCCCGGCC
  301 CGGCCGCGGG GAGCCCCACT TCATCGCAGT GGGCTACGTG GACGACACGC AGTTCGTGCG
  361 GTTCGACAGC GACGCCGCGA GTCCGAGAGG GGAGCCGCGG GCGCCGTGGG TGGAGCAGGA
  421 GGGGCCGGAG TATTGGGACC GGGAGACACA GAAGTACAAG CGCCAGGCAC AGACTGACCG
  481 AGTGAGCCTG CGGAACCTGC GCGGCTACTA CAACCAGAGC GAGGCCGGGT CTCACATCAT
  541 CCAGAGGATG TATGGCTGCG ACGTGGGGCC CGACGGGCGC CTCCTCCGCG GGTATGACCA
  601 GTACGCCTAC GACGGCAAGG ATTACATCGC CCTGAACGAG GATCTGCGCT CCTGGACCGC
  661 CGCGGACACG GCGGCTCAGA TCACCCAGCG CAAGTGGGAG GCGGCCCGTG ANGCGGANCA
  721 GCTGANAGCC TACCTGGANG GCCTGTGCGT GGAGTGGCTC CGCAGATACC TGAAGAATGN
  781 GAANGAGACG CTGCANCGCG CGGAACACCC AAAGACACAC GTGACCCACC ATCNNGTCTC
  841 TGACCATGAN GCCACCNNNA NGTGCTGGGC CCTGGGCTTC TANCCNGCNN ANNTCACACT
  901 GNNCNNNCNN NGGNNNGGGG GANGNNCAAA CTCNNNANNN NNNNNTTGNG GNNANCNNGC
961 CNGCNNNANN TGGNNNCNTT NNNNNNNNNG NNNNNNNNNG NNNNNNNCNN NN
//
Primer: BGH_rev2
Region: bGHp(A),insert3'
Sequence file: RDB17870_A9L2b.seq check
>02885_17870_A9L2_3_BGH_rev2_D06_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNGNNGGGNN NNNACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CGTCTCAGGC
  121 TTTACAAGCG ATGAGAGACT CATCAGAGCC CTGGGCACTG TTGCTGGACG CAGCCTGAGA
  181 GCAGCTCCCT CCTTTTCCAC CTGAGCTCTT CCTCCTACAC ATCACAACAG CCACCACAGC
  241 TCCTAGGACA GCTAGGACAG CCAGGACAGC CAGGCCAGCA ACGATGCCCA CGATGGGGAT
  301 GGTGGGCTGG GAAGACGGCT CCCATCTCAG GGTGAGGGGC TCCGGCAGCC CCTCGTGCTG
  361 CACATGGCAC GTGTATCTCT GCTCTTCTCC AGAAGGCACC ACCACAGCTG CCCACTTCTG
  421 GAAGGTTCCA TCTCCTGCTG GCCTGGTCTC CACAAGCTCA GTGTCCTGAG TTTGGTCCTC
  481 CCCATCCCAC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG AAGCCCAGGG CCCAGCACCT
  541 CAGGGTGGCC TCATGGTCAG AGACGGGATG GTGGGTCACG TGTGTCTTTG GGTGTTCCGC
  601 GCGCTGCAGC GTCTCCTTCC CATTCTTCAG GTATCTGCGG AGCCACTCCA CGCACAGGCC
  661 CTCCAGGTAG GCTCTCAGCT GCTCCGCCTC ACGGGCCGCC TCCCACTTGC GCTGGGTGAT
  721 CTGAGCCGCC GTGTCCGCGG CGGTCCAGGA NCGCAGATCC TCGTTCAGGG CGATGTAATC
  781 CTTGCCGTCG TANGCGTACT GGTCATACCC GCGGNNGANG CGCCCGTCNG GCCCCACGTC
  841 NCANCCATAC ATCCTCTNGA TGATGTNANA NCCGGCCTCG CTCNGGTTGT AGTANCCGCG
  901 CNNNNNNCGC NNNNTCNNNC NNNCANNCNG NGCCTGNNNC TNNNANTTCT GNNNNNNCNN
961 NNNCCNATNN NNNNGNNNNN NCNN
//
Primer: SV40pro_F
Sequence file: RDB17870_A9L2c.seq check
>02885_17870_A9L2_3_SV40pro_F_E06_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANNNNTGNNG NNGNTTTTTT GGANGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGNGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGNCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGNCATA GCGTTGGCTA CNCGTGATAT TGCTGAAGAN CTTGGCGGCG AATNNNCTGA
  841 CCGCTTCCTC NNGCTTTACG GTATCGCCGC TCCCNANNCN CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC NANTTCTTCN NANCGGNNNT NNNGGGGTNC NAAATGNNNN ANNAANCNNN
961 NCCNNANCNN GNCNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.
user report Banerjee, P.P., KIR2DL4-HLAG interaction at human NK cell-oligodendrocyte interfaces regulates IFN-gamma-mediated effects. Mol. Immunol. pii: S0161-5890 (18) 30835-6 (2018). PMID 30482463.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl