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Human HLA-C*03:03:01 cDNA

Human HLA-C*03:03:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02884
Resource name Human HLA-C*03:03:01 cDNA
Alternative name HLA-Cw*03031
Clone info. Human HLA-C*03:03:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*03031 cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02884 Human HLA-C*03:03:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02884z.seq from Depositor
Electronic file Map RDB02884.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13722_A5Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13722_A5Lla.seq
>02884_13722_A5Ll_T7_20bp_C05_08.ab1
    1 CGTACCAGGC GCTTACTTAG CTTTTCTCCC CAGACGCCGA GATGCGGGTC ATGGCGCCCC
   61 GAACCCTCAT CCTGCTGCTC TCGGGAGCCC TGGCCCTGAC CGAGACCTGG GCCGGCTCCC
  121 ACTCCATGAG GTATTTCTAC ACCGCTGTGT CCCGGCCCGG CCGCGGGGAG CCCCACTTCA
  181 TCGCAGTGGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC GCCGCGAGTC
  241 CGAGAGGGGA GCCGCGGGCG CCGTGGGTGG AGCAGGAGGG GCCGGAGTAT TGGGACCGGG
  301 AGACACAGAA GTACAAGCGC CAGGCACAGA CTGACCGAGT GAGCCTGCGG AACCTGCGCG
  361 GCTACTACAA CCAGAGCGAG GCCAGGTCTC ACATCATCCA GAGGATGTAT GGCTGCGACG
  421 TGGGGCCCGA CGGGCGCCTC CTCCGCGGGT ATGACCAGTA CGCCTACGAC GGCAAGGATT
  481 ACATCGCCCT GAACGAGGAT CTGCGCTCCT GGACCGCCGC GGACACGGCG GCTCAGATCA
  541 CCCAGCGCAA GTGGGAGGCG GCCCGTGAGG CGGAGCAGCT GAGAGCCTAC CTGGAGGGCC
  601 TGTGCGTGGA GTGGCTCCGC AGATACCTGA AGAATGGGAA GGAGACGCTG CAGCGCGCGG
  661 AACACCCAAA GACACACGTG ACCCACCATC CCGTCTCTGA CCATGAGGCC ACCCTGAGGT
721 GCTGGGCCCT GGGCTTCTAC CCTGCGGAGA TCACACTGAC CTGGCAGTG
//
Primer: BGH_rev RDB13722_A5Llb.seq
>02884_13722_A5Ll_BGHrev_D05_11.ab1
    1 GGGGACCGGG GGGCCTCTAA ACTCGAGCGG CCGCGTCTCA GGCTTTACAA GCGATGAGAG
   61 ACTCATCAGA GCCCTGGGCA CTGTTGCTGG ACGCAGCCTG AGAGCAGCTC CCTCCTTTTC
  121 CACCTGAGCT CTTCCTCCTA CACATCACAA CAGCCACCAC AGCTCCTAGG ACAGCTAGGA
  181 CAGCCAGGAC AGCCAGGCCA GCAACGATGC CCACGATGGG GATGGTGGGC TGGGAAGACG
  241 GCTCCCATCT CAGGGTGAGG GGCTCCGGCA GCCCCTCGTG CTGCACATGG CACGTGTATC
  301 TCTGCTCTTC TCCAGAAGGC ACCACCACAG CTGCCCACTT CTGGAAGGTT CCATCTCCTG
  361 CTGGCCTGGT CTCCACAAGC TCAGTGTCCT GAGTTTGGTC CTCCCCATCC CACTGCCAGG
  421 TCAGTGTGAT CTCCGCAGGG TAGAAGCCCA GGGCCCAGCA CCTCAGGGTG GCCTCATGGT
  481 CAGAGACGGG ATGGTGGGTC ACGTGTGTCT TTGGGTGTTC CGCGCGCTGC AGCGTCTCCT
  541 TCCCATTCTT CAGGTATCTG CGGAGCCACT CCACGCACAG GCCCTCCAGG TAGGCTCTCA
  601 GCTGCTCCGC CTCACGGGCC GCCTCCCACT TGCGCTGGGT GATCTGAGCC GCCGTGTCCG
  661 CGGCGGTCCA GGAGCGCAGA TCCTCGTTTC AGGGGCGATG TAATCCTTGC CGTCGTAGGC
721 GTACTGGGAC ATAACGCGCA GGAGGGAGGC GCCCTTCA
//
Primer: SV40pro_F RDB13722_A5Llc.seq
>02884_13722_A5Ll_SV40pro_F_E05_14.ab1
    1 CCCCGGTCCC TGGGCCATTT CCAGAATAAT GGAGGGAGGC TTTTTTGGAA GGCCCTAGGC
   61 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTATTC GGATCTGATC AAGAGACAGG
  121 ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
  181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
  241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
  301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
  361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
  421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
  481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
  541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
  601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
  661 GGCGCGCATG CCCGACGGCG GAGGGATCTC GTCGTGACCC ATGGGCGATG CCTGCTTGCC
  721 CGAAATATCA TGGTGGAAAA ATGGCCGCTT TTTCTGGATT TCATCGACTG TGGCCGGCTG
  781 GGGTGTGGCG GACCGCTATC AAGGACATAG GCGTTGGCTA CCCCGTGATA TTGCTGAAGA
  841 GCTTGGGCGG CGAATGGGGC TGACCGCTTC CCTCGTGCTT TACGGGTATC GCCGCCTCCC
  901 CGATTCGCAA GCGGCATCGC CTTTCTATCG GCCTTTCTTT GACGAAGTTT CTTCTGAGCG
  961 GGAACTCTGG GGGGTTTCGA AAATGACCGA ACCAGGCGAC GCCCCAAACC CTGGCCATCA
 1021 CACGAAGAAT TTTCTGAATT TCCCACCGGC CCGGACCTTT CTTATGGAAC AGGTTGGGGA
 1081 GCTTATCGGG AAGTTCGGTT TTTTTTCCCG GGGACCGCCT GGGCCTTTGG AAAGTGGATC
1141 CATCCCCGGA CCGCGCAGGA GGAATGTCCT CGTCAATT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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