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Human HLA-C*01:02 cDNA

Human HLA-C*01:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02883
Resource name Human HLA-C*01:02 cDNA
Alternative name HLA-Cw*0102
Clone info. Human HLA-C*01:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*0102 cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-C (NCBI Gene 3107) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02883 Human HLA-C*01:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02883z.seq
Electronic file Map RDB02883.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13699_A5Jrp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB13699_A5Jra.seq check
>02883_13699_A5Jr_CMV-Forward_A11_02.ab1
    1 CGGTCCCTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTTTCT CCCCAGACGC
  181 CGAGATGCGG GTCATGGCGC CCCGAACCCT CATCCTGCTG CTCTCGGGAG CCCTGGCCCT
  241 GACCGAGACC TGGGCCTGCT CCCACTCCAT GAAGTATTTC TTCACATCCG TGTCCCGGCC
  301 TGGCCGCGGA GAGCCCCGCT TCATCTCAGT GGGCTACGTG GACGACACGC AGTTCGTGCG
  361 GTTCGACAGC GACGCCGCGA GTCCGAGAGG GGAGCCGCGG GCGCCGTGGG TGGAGCAGGA
  421 GGGGCCGGAG TATTGGGACC GGGAGACACA GAAGTACAAG CGCCAGGCAC AGACTGACCG
  481 AGTGAGCCTG CGGAACCTGC GCGGCTACTA CAACCAGAGC GAGGCCGGGT CTCACACCCT
  541 CCAGTGGATG TGTGGCTGCG ACCTGGGGCC CGACGGGCGC CTCCTCCGCG GGTATGACCA
  601 GTACGCCTAC GACGGCAAGG ATTACATCGC CCTGAACGAG GACCTGCGCT CCTGGACCGC
  661 CGCGGACACC GCGGCTCAGA TCACCCAGCG CAAGTGGGAG GCGGCCCGTG AGGCGGAGCA
  721 GCGGAGAGCC TACCTGGAGG GCACGTGCGT GGAGTGGCTC CGCAGATACC TGGAGAACGG
  781 GAAGGAGACG CTGCAGCGCG CGGAACACCC AAAGACACAC GTGACCCACC ATCCCGTCTC
  841 TGACCATGAG GCCACCCTGA GGTGCTGGGC CCTGGGCTTC TACCCTGCGG AGATCACACT
  901 GACCTGGCAG TGGGATGGGG AGGACCAAAC TCAGGACACC GAGCTTGTGG AGACCAGGCC
  961 AGCAGGAGAT GGAAACCTTC CAGAAGTGGG CAGCTGTGAT GGGTGCCCTT CTGGAGAAGA
 1021 GCAGAGATAC ACGTGCCCAT GTGCAGCACG AGGGGGCTGC CCGGGAGCCC CCTTCAACCT
1081 TGAGATGGGT AGCCCATCCT TCCCAGACCC CAACCAATTC TCA
//
Primer: BGH_rev2
Region: insert 3'
Sequence file: RDB13699_A5Jrd.seq check
>02883_13699_A9L2F_BGH_rev2_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN TTAAACGGGC CCTCTAGACT CGAGCGGCCG CGTCTCAGGC
   61 TTTACAAGCG ATGAGAGACT CATCAGAGCC CTGGGCACTG TTGCTGGACG CAGCCTGAGA
  121 GCAGCTCCCT CCTTTTCCAC CTGAGCTCTT CCTCCTACAC ATCACAACAG CCACCACAGC
  181 TCCTAGGACA GCTAGGACAG CCAGGACAGC CAGGCCAGCA ACGATGCCCA CGATGGGGAT
  241 GGTGGGCTGG GAAGACGGCT CCCATCTCAG GGTGAGGGGC TCCGGCAGCC CCTCGTGCTG
  301 CACATGGCAC GTGTATCTCT GCTCTTCTCC AGAAGGCACC ATCACAGCTG CCCACTTCTG
  361 GAAGGTTCCA TCTCCTGCTG GCCTGGTCTC CACAAGCTCG GTGTCCTGAG TTTGGTCCTC
  421 CCCATCCCAC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG AAGCCCAGGG CCCAGCACCT
  481 CAGGGTGGCC TCATGGTCAG AGACGGGATG GTGGGTCACG TGTGTCTTTG GGTGTTCCGC
  541 GCGCTGCAGC GTCTCCTTCC CGTTCTCCAG GTATCTGCGG AGCCACTCCA CGCACGTGCC
  601 CTCCAGGTAG GCTCTCCGCT GCTCCGCCTC ACGGGCCGCC TCCCACTTGC GCTGGGTGAT
  661 CTGAGCCGCG GTGTCCGCGG CGGTCCAGGA NCGCAGGTCC TCGTTCAGGG CGATGTAATC
  721 CTTGCCGTCG TANGCGTACT GGTCATACCC GCGGANGAGG CGCCCGNCGG GCCCCANGNC
  781 GCAGCCACAC ATCCNCTGNA NGGNGTGAGA CCCGGCCTCG CTCTGGTTGT AGTANCCGCN
  841 CANGTTNNGC ANGCTCACTC NGGTCNGTCT GTGCNNGNNG CTTGNACNTT NNGTGTCTNC
901 NNGGNCCCAN NANNCCNGNC CCNNNNNGNN NCANCCANNN NNNNNNN
//
Primer: SV40pro_F_V2
Region: NeoR
Sequence file: RDB13699_A5Jrc.seq check
>02883_13699_A5Jr_SV40pro_F_V2_C11_08.ab1
    1 GTAGGCCTTT TTGGAGGCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
   61 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
  121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
  181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
  241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
  301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
  361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
  421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
  481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
  541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
  601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
  661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
  721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
  781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
  841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
  901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC
  961 ACCCGCCGCC CTTCTATGAA AGGGTTGGGC TTCGGAATCG TTTTTCCGGG ACGCCGGGCT
 1021 GGATGATCCC TCCAGCGCGG GGGATCTCAT GCCTGGAGTT CCTTCGGCCC AACCCCCAAA
1081 CCTGTTACTG GCCAGCCTTA TAAATTGCTT TAACAAATTA AAGGCCAATT AGGTCAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.
user report Ichise, H., NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs. Stem Cell Reports 9 (3): 853-867 (2017). PMID 28867344.

2020.03.03

GNP_filter3_RDBDEP_html_200228.pl