Resource data sheet
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Human HLA-B*52:01:01 cDNA

Human HLA-B*52:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02882
Resource name Human HLA-B*52:01:01 cDNA
Alternative name HLA-B*52011
Clone info. Human HLA-B*52:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02882 Human HLA-B*52:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02882z.seq from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02882_A2Kip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB02882_A2Kia.seq
>02882_02882_A2Ki_T7_20bp_E08_14.ab1
    1 CAAGCTGGCT AGCGTTTAAA CTTAGCTTCA CCCGGACTCA GAATCTCCTC AGACGCCGAG
   61 ATGCGGGTCA CGGCGCCCCG AACCGTCCTC CTGCTGCTCT GGGGGGCAGT GGCCCTGACC
  121 GAGACCTGGG CCGGCTCCCA CTCCATGAGG TATTTCTACA CCGCCATGTC CCGGCCCGGC
  181 CGCGGGGAGC CCCGCTTCAT CGCAGTGGGC TACGTGGACG ACACCCAGTT CGTGAGGTTC
  241 GACAGCGACG CCGCGAGTCC GAGGACGGAG CCCCGGGCGC CATGGATAGA GCAGGAGGGG
  301 CCGGAGTATT GGGACCGGGA GACACAGATC TCCAAGACCA ACACACAGAC TTACCGAGAG
  361 AACCTGCGGA TCGCGCTCCG CTACTACAAC CAGAGCGAGG CCGGGTCTCA CACTTGGCAG
  421 ACGATGTATG GCTGCGACGT GGGGCCGGAC GGGCGCCTCC TCCGCGGGCA TAACCAGTAC
  481 GCCTACGACG GCAAAGATTA CATCGCCCTG AACGAGGACC TGAGCTCCTG GACCGCGGCG
  541 GACACCGCGG CTCAGATCAC CCAGCGCAAG TGGGAGGCGG CCCGTGAGGC GGAGCAGCTG
  601 AGAGCCTACC TGGAGGGCCT GTGCGTGGAG TGGCTCCGCA GACACCTGGA GAACGGGAAG
  661 GAGACGCTGC AGCGCGCGGA CCCCCCAAAG ACACACGTGA CCCACCACCC CGTCTCTGAC
  721 CATGAGGCCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGCGGAGAT CACACTGACC
  781 TGGCAGCGGG ATGGCGAGGA CCAAACTCAG GACACTGAGC TTGTGGAGAC CAGACCAGCA
  841 GGAGATAGAA CCTTCCAGAA GTGGGCAGCT GTGGTGGTGC CTTCTGGAGA AGAGCAGAGA
  901 TACACATGCC ATGTACAGCA TGAGGGGCTG CCGAAGCCCC TCACCCTGAG ATGGGAGCCA
  961 TCTTCCCAGT CCACCATCCC CATCGTGGGC ATTGTTGCTG GCCTGGCTGT CCCTAGCAGT
 1021 TGTGGTCATC GGGAGCTGTG GGTCGCTACT GTGAATGTGT AGGAGGAAGA AGCTCAGTGG
 1081 AAAAGGAGGG AGCTACTCTC AGGCTGCGTC AGCGACAGTG CCAGGGCTCT GAAATGGTGT
1141 CCTCCTCCAA CAAGCC
//
Primer: BGH_rev RDB02882_A2Kib.seq
>02882_02882_A2Ki_BGHrev_F08_17.ab1
    1 GATTCGGGAC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC TCACAAGACA GCTGTCTCAG
   61 GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC TGTCGCTGGA CGCAGCCTGA
  121 GAGTAGCTCC CTCCTTTTCC ACCTGAGCTC TTCCTCCTAC ACATCACAGT AGCGACCACA
  181 GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA CAATGCCCAC GATGGGGATG
  241 GTGGACTGGG AAGATGGCTC CCATCTCAGG GTGAGGGGCT TCGGCAGCCC CTCATGCTGT
  301 ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA CCACAGCTGC CCACTTCTGG
  361 AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG TGTCCTGAGT TTGGTCCTCG
  421 CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA AGCCCAGGGC CCAGCACCTC
  481 AGGGTGGCCT CATGGTCAGA GACGGGGTGG TGGGTCACGT GTGTCTTTGG GGGGTCCGCG
  541 CGCTGCAGCG TCTCCTTCCC GTTCTCCAGG TGTCTGCGGA GCCACTCCAC GCACAGGCCC
  601 TCCAGGTAGG CTCTCAGCTG CTCCGCCTCA CGGGCCGCCT CCCACTTGCG CTGGGTGATC
  661 TGAGCCGCGG TGTCCGCCGC GGTCCAGGAG CTCAGGTCCT CGTTCAGGGC GATGTAATCT
  721 TTGCCGTCGT AGGCGTACTG GTTATGCCCG CGGAGGAGGC GCCCGTCCGG CCCCACGTCG
  781 CAGCCATACA TCGTCTGCCA AGTGTGAGAC CCGGCCTCGC TCTGGTTGTA GTAGCGGAGC
  841 GCGATCCGCA GGTTCTCTCG GTAAGTCTGT GTGTTGGTCT TGGAGATCTG TGTCTCCCGG
  901 TCCCAATACT CCGGCCCCTC CTGCTCTATC CATGGCGCCC GGGGCTCCGT CCTCGGACTC
  961 GCGGCGTCGC TGTCGAACCT CACGAACTGG GTGTCGTCCA CGTAGCCCAC TGCGATGAAG
 1021 CGGGGCTCCC CGCGGCCCGG GCCGGGACAT GGCGGTGTAG AAAATACCCT CATGGAGTGG
 1081 AGCCGGCCCA GGGTCTCGGG TCAGACACTG GCCCCCCAGA GCCAGCAAGA GGACGGATTC
1141 GGGGCCGCCC GGGTGAATCT TG
//
Primer: SV40pro_F RDB02882_A2Kic.seq
>02882_02882_A2Ki_SV40pro_F_D02_11.ab1
    1 CACCGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGAATC AGGATGATCT
  601 GGACGAAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGCAT GCCTGCTTTG CCGAAATATC
  721 ATGGTGGAAA ATGGGCCGCT TTTTCTGGAA TTCTTCGAGT GTGGGACAGG CGTGGGGGTG
781 GGCGGAAGCT CCTATCAAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl