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Human HLA-B*52:01:01 cDNA

Human HLA-B*52:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02882
Resource name Human HLA-B*52:01:01 cDNA
Alternative name HLA-B*52011
Clone info. Human HLA-B*52:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          RDB02882 Human HLA-B*52:01:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02882z.seq

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18216_B0F2p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB18216_B0F2a.seq check
          >02882_18216_B0F2_2_CMV_Forward_A08_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTTG NNGCNATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAGCTTCAC CCGGACTCAG
            181 AATCTCCTCA GACGCCGAGA TGCGGGTCAC GGCGCCCCGA ACCGTCCTCC TGCTGCTCTG
            241 GGGGGCAGTG GCCCTGACCG AGACCTGGGC CGGCTCCCAC TCCATGAGGT ATTTCTACAC
            301 CGCCATGTCC CGGCCCGGCC GCGGGGAGCC CCGCTTCATC GCAGTGGGCT ACGTGGACGA
            361 CACCCAGTTC GTGAGGTTCG ACAGCGACGC CGCGAGTCCG AGGACGGAGC CCCGGGCGCC
            421 ATGGATAGAG CAGGAGGGGC CGGAGTATTG GGACCGGGAG ACACAGATCT CCAAGACCAA
            481 CACACAGACT TACCGAGAGA ACCTGCGGAT CGCGCTCCGC TACTACAACC AGAGCGAGGC
            541 CGGGTCTCAC ACTTGGCAGA CGATGTATGG CTGCGACGTG GGGCCGGACG GGCGCCTCCT
            601 CCGCGGGCAT AACCAGTACG CCTACGACGG CAAAGATTAC ATCGCCCTGA ACGAGGACCT
            661 GAGCTCCTGG ACCGCGGCGG ACACCGCGGC TCANATCACC CAGCGCAAGT GGGAGGCGGC
            721 CCGTGNNGCG GANCAGCTGA NAGCCTACCT GGAGGGCCTG TGCGTGGANT GGCTCCGCAG
            781 ACACCTGNAG AACGGGAAGG AGANGCTGCA GCGCGCGGAN CCCCCNAAGA NACACGTGAN
            841 CCACCACCCC GTCTCTGANC NTGANGNCNC CCTGANGNGC TGGGCCCNGG GNTTCNANCC
            901 TGNGGANATC ACNNNGNNNN NNNNNCGGGN TGGNNANGAN CNAACTCNNN NNCNNANCTN
          961 GNNNNNANNN NANCANCNNN ANANANAANN TTNNANNNNG GNNAGCNNNN NNNNNN
          //
          Primer: bGH_rev3
          Region: bGHpA,insert 3'
          Sequence file: RDB18216_B0F2b.seq check
          >02882_18216_B0F2_2_bGH_rev3_C08_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGGNNGGG CNNACNACAG ATGGCTGGCA ACTAGAAGGC
             61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCATCTCA
            121 GTCCCTCACA AGACAGCTGT CTCAGGCTTT TCAAGCTGTG AGAGACACAT CAGAGCCCTG
            181 GGCACTGTCG CTGGACGCAG CCTGAGAGTA GCTCCCTCCT TTTCCACCTG AGCTCTTCCT
            241 CCTACACATC ACAGTAGCGA CCACAGCTCC GATGACCACA ACTGCTAGGA CAGCCAGGCC
            301 AGCAACAATG CCCACGATGG GGATGGTGGA CTGGGAAGAT GGCTCCCATC TCAGGGTGAG
            361 GGGCTTCGGC AGCCCCTCAT GCTGTACATG GCATGTGTAT CTCTGCTCTT CTCCAGAAGG
            421 CACCACCACA GCTGCCCACT TCTGGAAGGT TCTATCTCCT GCTGGTCTGG TCTCCACAAG
            481 CTCAGTGTCC TGAGTTTGGT CCTCGCCATC CCGCTGCCAG GTCAGTGTGA TCTCCGCAGG
            541 GTAGAAGCCC AGGGCCCAGC ACCTCAGGGT GGCCTCATGG TCAGAGACGG GGTGGTGGGT
            601 CACGTGTGTC TTTGGGGGGT CCGCGCGCTG CAGCGTCTCC TTCCCGTTCT CCAGGTGTCT
            661 GCGGAGCCAC TCCACGCACA GGCCCTCCAG GTAGGCTCTC AGCTGCTCCG CCTCACGGGC
            721 CGCCTCCCAC TTGCGCTGGG TGATCTGAGC CGCGGTGTCC GCCGCGGTCC AGGANCTCAN
            781 GTCCTCGTTC ANGGCGATGT AATCTTTGCC GTCGTANGCG TACTGGTTAT GCCCGCGGAN
            841 GNNGCGCCCG TCCGGCCCCA CGTCNCANCC ATACATCGTC TGNCNAGTGT GAGANCCGGC
            901 CTCGCNNTGG TTGTANTANC GNANCGCGNT NCGNNNNTTC TNNCNGNAAG TCTGNGNGTT
            961 GGNCTNNGNA NNTNTGNGTC NNCCGGNCNN NNNANNNCNN NCCNNNCTGC TNNNTCCATG
          1021 GNNNCNNGGG NNNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18216_B0F2c.seq check
          >02882_18216_B0F2_2_SV40pro_ori_F_E08_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNCNC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
             61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
            121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
            181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
            241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
            301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
            361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
            421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
            481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
            541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
            601 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
            661 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGNTC TCGTCGTGAC CCATGGCGAT
            721 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
            781 CGGCTGGGTG TGGCGGANCG CTATCANGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
            841 GAGCTTGNCG GCGAATNNNC TGACCGCTTC CTCGNGCTTT ACGGTATCGC CGCNCCCGAN
            901 TCGCANCGCA TCGCCNTCNA TCGCCTTCNT GNNNAGTNCN TCNGANCGGG NNTCTGGGGT
            961 TCNAAATGAC CNANCAANCG NNNCCNNNNC NGNNNTCNCN ANNNTNCNAN TNNNNNGNCN
          1021 NCNNNNNNNN NANNNNNNGN NNTNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl