Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-B*52:01:01 cDNA

Human HLA-B*52:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02882
Resource name Human HLA-B*52:01:01 cDNA
Alternative name HLA-B*52011
Clone info. Human HLA-B*52:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02882 Human HLA-B*52:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02882z.seq

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18216_B0F2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB18216_B0F2a.seq check
>02882_18216_B0F2_2_CMV_Forward_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG NNGCNATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAGCTTCAC CCGGACTCAG
  181 AATCTCCTCA GACGCCGAGA TGCGGGTCAC GGCGCCCCGA ACCGTCCTCC TGCTGCTCTG
  241 GGGGGCAGTG GCCCTGACCG AGACCTGGGC CGGCTCCCAC TCCATGAGGT ATTTCTACAC
  301 CGCCATGTCC CGGCCCGGCC GCGGGGAGCC CCGCTTCATC GCAGTGGGCT ACGTGGACGA
  361 CACCCAGTTC GTGAGGTTCG ACAGCGACGC CGCGAGTCCG AGGACGGAGC CCCGGGCGCC
  421 ATGGATAGAG CAGGAGGGGC CGGAGTATTG GGACCGGGAG ACACAGATCT CCAAGACCAA
  481 CACACAGACT TACCGAGAGA ACCTGCGGAT CGCGCTCCGC TACTACAACC AGAGCGAGGC
  541 CGGGTCTCAC ACTTGGCAGA CGATGTATGG CTGCGACGTG GGGCCGGACG GGCGCCTCCT
  601 CCGCGGGCAT AACCAGTACG CCTACGACGG CAAAGATTAC ATCGCCCTGA ACGAGGACCT
  661 GAGCTCCTGG ACCGCGGCGG ACACCGCGGC TCANATCACC CAGCGCAAGT GGGAGGCGGC
  721 CCGTGNNGCG GANCAGCTGA NAGCCTACCT GGAGGGCCTG TGCGTGGANT GGCTCCGCAG
  781 ACACCTGNAG AACGGGAAGG AGANGCTGCA GCGCGCGGAN CCCCCNAAGA NACACGTGAN
  841 CCACCACCCC GTCTCTGANC NTGANGNCNC CCTGANGNGC TGGGCCCNGG GNTTCNANCC
  901 TGNGGANATC ACNNNGNNNN NNNNNCGGGN TGGNNANGAN CNAACTCNNN NNCNNANCTN
961 GNNNNNANNN NANCANCNNN ANANANAANN TTNNANNNNG GNNAGCNNNN NNNNNN
//
Primer: bGH_rev3
Region: bGHpA,insert 3'
Sequence file: RDB18216_B0F2b.seq check
>02882_18216_B0F2_2_bGH_rev3_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGGNNGGG CNNACNACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCATCTCA
  121 GTCCCTCACA AGACAGCTGT CTCAGGCTTT TCAAGCTGTG AGAGACACAT CAGAGCCCTG
  181 GGCACTGTCG CTGGACGCAG CCTGAGAGTA GCTCCCTCCT TTTCCACCTG AGCTCTTCCT
  241 CCTACACATC ACAGTAGCGA CCACAGCTCC GATGACCACA ACTGCTAGGA CAGCCAGGCC
  301 AGCAACAATG CCCACGATGG GGATGGTGGA CTGGGAAGAT GGCTCCCATC TCAGGGTGAG
  361 GGGCTTCGGC AGCCCCTCAT GCTGTACATG GCATGTGTAT CTCTGCTCTT CTCCAGAAGG
  421 CACCACCACA GCTGCCCACT TCTGGAAGGT TCTATCTCCT GCTGGTCTGG TCTCCACAAG
  481 CTCAGTGTCC TGAGTTTGGT CCTCGCCATC CCGCTGCCAG GTCAGTGTGA TCTCCGCAGG
  541 GTAGAAGCCC AGGGCCCAGC ACCTCAGGGT GGCCTCATGG TCAGAGACGG GGTGGTGGGT
  601 CACGTGTGTC TTTGGGGGGT CCGCGCGCTG CAGCGTCTCC TTCCCGTTCT CCAGGTGTCT
  661 GCGGAGCCAC TCCACGCACA GGCCCTCCAG GTAGGCTCTC AGCTGCTCCG CCTCACGGGC
  721 CGCCTCCCAC TTGCGCTGGG TGATCTGAGC CGCGGTGTCC GCCGCGGTCC AGGANCTCAN
  781 GTCCTCGTTC ANGGCGATGT AATCTTTGCC GTCGTANGCG TACTGGTTAT GCCCGCGGAN
  841 GNNGCGCCCG TCCGGCCCCA CGTCNCANCC ATACATCGTC TGNCNAGTGT GAGANCCGGC
  901 CTCGCNNTGG TTGTANTANC GNANCGCGNT NCGNNNNTTC TNNCNGNAAG TCTGNGNGTT
  961 GGNCTNNGNA NNTNTGNGTC NNCCGGNCNN NNNANNNCNN NCCNNNCTGC TNNNTCCATG
1021 GNNNCNNGGG NNNNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB18216_B0F2c.seq check
>02882_18216_B0F2_2_SV40pro_ori_F_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNCNC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
  121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
  181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
  241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
  301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
  361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
  421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
  481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
  541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
  601 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
  661 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGNTC TCGTCGTGAC CCATGGCGAT
  721 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
  781 CGGCTGGGTG TGGCGGANCG CTATCANGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
  841 GAGCTTGNCG GCGAATNNNC TGACCGCTTC CTCGNGCTTT ACGGTATCGC CGCNCCCGAN
  901 TCGCANCGCA TCGCCNTCNA TCGCCTTCNT GNNNAGTNCN TCNGANCGGG NNTCTGGGGT
  961 TCNAAATGAC CNANCAANCG NNNCCNNNNC NGNNNTCNCN ANNNTNCNAN TNNNNNGNCN
1021 NCNNNNNNNN NANNNNNNGN NNTNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl