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Human HLA-B*51:01:01 cDNA

Human HLA-B*51:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02881
Resource name Human HLA-B*51:01:01 cDNA
Alternative name HLA-B*51011
Clone info. Human HLA-B*51:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02881 Human HLA-B*51:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02881z.seq
Electronic file Map RDB02881.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13720_A5Llp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: insert 5'
Sequence file: RDB13720_A5Lld.seq check
>02881_13720_A9L2F_CMV-Forward_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN NNNNNTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA
   61 ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC
  121 CGGACTCAGA ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCGCCCCGAA CCGTCCTCCT
  181 GCTGCTCTGG GGGGCAGTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA
  241 TTTCTACACC GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATTG CAGTGGGCTA
  301 CGTGGACGAC ACCCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GGACGGAGCC
  361 CCGGGCGCCA TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGAACA CACAGATCTT
  421 CAAGACCAAC ACACAGACTT ACCGAGAGAA CCTGCGGATC GCGCTCCGCT ACTACAACCA
  481 GAGCGAGGCC GGGTCTCACA CTTGGCAGAC GATGTATGGC TGCGACGTGG GGCCGGACGG
  541 GCGCCTCCTC CGCGGGCATA ACCAGTACGC CTACGACGGC AAAGATTACA TCGCCCTGAA
  601 CGAGGACCTG AGCTCCTGGA CCGCGGCGGA CACCGCGGCT CANATCACCC AGCGCAAGTG
  661 GGAGGCGGCC CGTGAGGCGG AGCAGCTGAG AGCCTACCTG GAGGGCCTGT GCGTGGAGTG
  721 NCTCCGCAGA CACCTGGAGA ACGGGAAGGA NANGCTGCAG CGCGCGGANC CCCCNNAGAC
  781 ACACGTGANC CACNNCCCCG TCTCTGANNN TGANGCCANC CTGAGGTGCT GGGCCCNNGG
  841 CTTCTACCCT GNNGAGATCA CNCNGANNTN NNNNCNGGAN GGCGANGNAN CNAANCTCNN
  901 GNNNNCNNAG CTNGTGNANN NCANACNNNN NNGNANANAG AANCNTNNCN NAANNNGNNN
961 NNCTNNGNNG NNGNCNTTCT NNNNAANANC NNNNNNNNNN
//
Primer: BGH_rev2
Region: insert 3'
Sequence file: RDB13720_A5Lle.seq check
>02881_13720_A9L2F_BGH_rev2_E02_14_ABI24.ab1
    1 NNNNNNNNNN CNNNNNNNNN NCTNGNNNNN NNGGGTTTAA ACGGGCCCTC TAGACTCGAG
   61 CGGCCGCATC TCAGTCCCTC ACAAGACAGC TGTCTCAGGC TTTTCAAGCT GTGAGAGACA
  121 CATCAGAGCC CTGGGCACTG TCGCTGGACG CAGCCTGAGA GTAGCTCCCT CCTTTTCCAC
  181 CTGAGCTCTT CCTCCTACAC ATCACAGTAG CGACCACAGC TCCGATGACC ACAACTGCTA
  241 GGACAGCCAG GCCAGCAACA ATGCCCACGA TGGGGATGGT GGACTGGGAA GATGGCTCCC
  301 ATCTCAGGGT GAGGGGCTTC GGCAGCCCCT CATGCTGTAC ATGGCATGTG TATCTCTGCT
  361 CTTCTCCAGA AGGCACCACC ACAGCTGCCC ACTTCTGGAA GGTTCTATCT CCTGCTGGTC
  421 TGGTCTCCAC AAGCTCAGTG TCCTGAGTTT GGTCCTCGCC ATCCCGCTGC CAGGTCAGTG
  481 TGATCTCCGC AGGGTAGAAG CCCAGGGCCC AGCACCTCAG GGTGGCCTCA TGGTCAGAGA
  541 CGGGGTGGTG GGTCACGTGT GTCTTTGGGG GGTCCGCGCG CTGCAGCGTC TCCTTCCCGT
  601 TCTCCAGGTG TCTGCGGAGC CACTCCACGC ACAGGCCCTC CAGGTAGGCT CTCAGCTGCT
  661 CCGCCTCACG GGCCGCCTCC CACTTGCGCT GGGTGATCTG AGCCGCGGTG TCCGCCGCGG
  721 TCCAGGAGCT CAGGTCCTCG TTCANGGCGA TGTAATCTTT GCCGTCGTAN GCGTACTGGT
  781 TATGCCCGCG GANGANGCGC CCGTCCGGCN NNACGTCGCA GCCATACATC GTCTGCCAAG
  841 NGTGAGANCC GGCCTCGCTN TGGTTGTANT ANCNGANCGC GATCCGCANN TTNNCTCGGN
  901 AAGNNTGTGT GTTGGNNTNG AAGANCTGNG TGTTCCNGTN CNNNNANTCN NGNCCNNNCN
961 NNNNNATCCN TGGNGNCNNG GGNNNNNNNN NN
//
Primer: SV40pro_F
Region: SV40pro_ori, NeoR
Sequence file: RDB13720_A5Llf.seq check
>02881_13720_A5Ll_SV40pro_F_B05_05.ab1
    1 CCCGGGCCCC TGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TTCGAAATGA CCGACCAAGC
  961 GACGCCCAAC CCTGCCATCA CGAGATTTTC GATTCCCACC GCCGCCTTTC TAATGGAAGA
 1021 TTGGGGCTTC GGCATCGGTT TTCGGACGGT GGCTGGATGA ATCCTCCAGC GCGGGGGAAT
1081 TCCTCCAT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.03.03

GNP_filter3_RDBDEP_html_200228.pl