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Human HLA-B*51:01:01 cDNA

Human HLA-B*51:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02881
Resource name Human HLA-B*51:01:01 cDNA
Alternative name HLA-B*51011
Clone info. Human HLA-B*51:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02881 Human HLA-B*51:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02881z.seq
Electronic file Map RDB02881.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18215_B0F2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB18215_B0F2a.seq check
>02881_18215_B0F2_1_CMV_Forward_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNTTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTCACCC GGACTCAGAA
  181 TCTCCTCAGA CGCCGAGATG CGGGTCACGG CGCCCCGAAC CGTCCTCCTG CTGCTCTGGG
  241 GGGCAGTGGC CCTGACCGAG ACCTGGGCCG GCTCCCACTC CATGAGGTAT TTCTACACCG
  301 CCATGTCCCG GCCCGGCCGC GGGGAGCCCC GCTTCATTGC AGTGGGCTAC GTGGACGACA
  361 CCCAGTTCGT GAGGTTCGAC AGCGACGCCG CGAGTCCGAG GACGGAGCCC CGGGCGCCAT
  421 GGATAGAGCA GGAGGGGCCG GAGTATTGGG ACCGGAACAC ACAGATCTTC AAGACCAACA
  481 CACAGACTTA CCGAGAGAAC CTGCGGATCG CGCTCCGCTA CTACAACCAG AGCGAGGCCG
  541 GGTCTCACAC TTGGCAGACG ATGTATGGCT GCGACGTGGG GCCGGACGGG CGCCTCCTCC
  601 GCGGGCATAA CCAGTACGCC TACGACGGCA AAGATTACAT CGCCCTGAAC GAGGACCTGA
  661 GCTCCTGGAC CGCGGCGGAC ACCGCGGCTC AGATCACCCA GCGCAAGTGG GAGGCGGCCC
  721 GTGNGGCGGA GCAGCTGAGA GCCTACCTGG AGGGCCTGTG CGTGGAGTGG CTCCGCAGAC
  781 ACCTGGAGAA CGGGAAGGAG ANGCTGCAGC GCGCGGACCC CCCNAAGACA CACGTGACCC
  841 ACCACCCCGT CTCTGANCAT GANGCCACCC TGANGTGCTG GGCCCTGGGC TTCNANCCTG
  901 CGGANATCAC ACNNNNCTGN CAGCGGGNTG GCGANGNNCN AACTCNNNNA CNNANCTTGN
  961 GGANNNCAGA NCAGCNNNNA NANNNNCTTC CANNNNNNGG CNNCNNNNGN NGNNGNCNTN
1021 NNNNNNNANA NNNNNNNNNN
//
Primer: bGH_rev3
Region: bGHpA,insert 3'
Sequence file: RDB18215_B0F2b.seq check
>02881_18215_B0F2_1_bGH_rev3_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GGGGNNGGNN AANAACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGCGGCC GCATCTCAGT
  121 CCCTCACAAG ACAGCTGTCT CAGGCTTTTC AAGCTGTGAG AGACACATCA GAGCCCTGGG
  181 CACTGTCGCT GGACGCAGCC TGAGAGTAGC TCCCTCCTTT TCCACCTGAG CTCTTCCTCC
  241 TACACATCAC AGTAGCGACC ACAGCTCCGA TGACCACAAC TGCTAGGACA GCCAGGCCAG
  301 CAACAATGCC CACGATGGGG ATGGTGGACT GGGAAGATGG CTCCCATCTC AGGGTGAGGG
  361 GCTTCGGCAG CCCCTCATGC TGTACATGGC ATGTGTATCT CTGCTCTTCT CCAGAAGGCA
  421 CCACCACAGC TGCCCACTTC TGGAAGGTTC TATCTCCTGC TGGTCTGGTC TCCACAAGCT
  481 CAGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT CAGTGTGATC TCCGCAGGGT
  541 AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC AGAGACGGGG TGGTGGGTCA
  601 CGTGTGTCTT TGGGGGGTCC GCGCGCTGCA GCGTCTCCTT CCCGTTCTCC AGGTGTCTGC
  661 GGAGCCACTC CACGCACAGG CCCTCCAGGT AGGCTCTCAG CTGCTCCGCC TCACGGGCCG
  721 CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CGGTGTCCGC CGCGGTCCAN GANCTCANGT
  781 CCTCGTTCAN GGCGATGTAA TCTTTGCCGT CNTANGCGTA NTGNTTATGC CCGCGGANGA
  841 NGCGCCCGTC CGGCCCCACG TCGCANCNNT ACATCGTCTG NCNNNTGTGA NANCCGGCCN
  901 CGCNCTGGTT GTANTANCGG ANCNCGNTNC NNNNNTCNNT CGGNAAGTCT GNGTGTNGGT
  961 CTTGNNNNNC TGNGTGTNNN NGNCCNNNNA NNNCNNNNCN NNNNNGNNNT ATCCATNNNN
1021 NCNNGGNNNN CNNNNNNNGN NNNNNNNGNN NNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB18215_B0F2c.seq check
>02881_18215_B0F2_1_SV40pro_ori_F_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGCCN CCNCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
  181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
  241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
  301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
  361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
  421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
  481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
  541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
  601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
  661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGANGAT CTCGTCGTGA CCCATGGCGA
  721 TGCCTGCTTG CCGAATATCA TGGTGGANNA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
  781 CCGGCTGGGT GTGGCGGANC GCTATCANGA NATANCGTTG GCTACCCGTG ATATTGCTGN
  841 ANANCTTGGC GGCGAANGGN CTGANCGCTT CCTCGTGCTT TACNGNATCG CCGCNCCNNA
  901 NNNNCANCGC ATCGCNNTCN ATCGCNNTCN NGNNNANNNC NNCNNANCNG GNNTNNNGGN
961 NNCNAAATGA NNNANNNNNC GANNNCNNNN CNNNNNNTNN NNNNNNTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl