Resource data sheet
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Human HLA-B*51:01:01 cDNA

Human HLA-B*51:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02881
Resource name Human HLA-B*51:01:01 cDNA
Alternative name HLA-B*51011
Clone info. Human HLA-B*51:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02881 Human HLA-B*51:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02881z.seq from Depositor
Electronic file Map RDB02881.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13720_A5Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13720_A5Lla.seq
>02881_13720_A5Ll_T7_20bp_H04_22.ab1
    1 CGACCATCCG ATTACTTAGC TTCACCCGGA CTCAGAATCT CCTCAGACGC CGAGATGCGG
   61 GTCACGGCGC CCCGAACCGT CCTCCTGCTG CTCTGGGGGG CAGTGGCCCT GACCGAGACC
  121 TGGGCCGGCT CCCACTCCAT GAGGTATTTC TACACCGCCA TGTCCCGGCC CGGCCGCGGG
  181 GAGCCCCGCT TCATTGCAGT GGGCTACGTG GACGACACCC AGTTCGTGAG GTTCGACAGC
  241 GACGCCGCGA GTCCGAGGAC GGAGCCCCGG GCGCCATGGA TAGAGCAGGA GGGGCCGGAG
  301 TATTGGGACC GGAACACACA GATCTTCAAG ACCAACACAC AGACTTACCG AGAGAACCTG
  361 CGGATCGCGC TCCGCTACTA CAACCAGAGC GAGGCCGGGT CTCACACTTG GCAGACGATG
  421 TATGGCTGCG ACGTGGGGCC GGACGGGCGC CTCCTCCGCG GGCATAACCA GTACGCCTAC
  481 GACGGCAAAG ATTACATCGC CCTGAACGAG GACCTGAGCT CCTGGACCGC GGCGGACACC
  541 GCGGCTCAGA TCACCCAGCG CAAGTGGGAG GCGGCCCGTG AGGCGGAGCA GCTGAGAGCC
  601 TACCTGGAGG GCCTGTGCGT GGAGTGGCTC CGCAGACACC TGGAGAACGG GAAGGAGACG
  661 CTGCAGCGCG CGGACCCCCC AAAGACACAC GTGACCCACC ACCCCGTCTC TGACCATGAG
  721 GCCACCCTGA GGTGCTGGGC CCTGGGCTTC TACCCTGCGG AGATCACACT GACCTGGCAG
  781 CGGGATGGCG AGGACCAAAC TCAGGACACT GAGCTTGTGG AGACCAGACC AGCAGGAGAA
  841 TAAGAACCTT TCCAGAAGTG GGCAGCTGTG GTGGTGCCTT CTGGAGAAGA AGCAGAGATA
  901 CACATGCCCA TGTACAGCAT GAGGGGCTGC CGAAAGCCCC CTCACCCTGA GATGGGAGCA
  961 TCTCCCAGTT CAACATCCCC ATCGTGGGGC CATGGAGCTT GCTGCTGTCT AGCCAGTTGT
1021 GGTCATCGGA CTGTGTCGCT ACTGTGAAGG GTA
//
Primer: BGH_rev RDB13720_A5Llb.seq
>02881_13720_A5Ll_BGHrev_A05_02.ab1
    1 GTAGGTACGG GCGTCTAGAC TCGAGCGGCC GCATCTCAGT CCCTCACAAG ACAGCTGTCT
   61 CAGGCTTTTC AAGCTGTGAG AGACACATCA GAGCCCTGGG CACTGTCGCT GGACGCAGCC
  121 TGAGAGTAGC TCCCTCCTTT TCCACCTGAG CTCTTCCTCC TACACATCAC AGTAGCGACC
  181 ACAGCTCCGA TGACCACAAC TGCTAGGACA GCCAGGCCAG CAACAATGCC CACGATGGGG
  241 ATGGTGGACT GGGAAGATGG CTCCCATCTC AGGGTGAGGG GCTTCGGCAG CCCCTCATGC
  301 TGTACATGGC ATGTGTATCT CTGCTCTTCT CCAGAAGGCA CCACCACAGC TGCCCACTTC
  361 TGGAAGGTTC TATCTCCTGC TGGTCTGGTC TCCACAAGCT CAGTGTCCTG AGTTTGGTCC
  421 TCGCCATCCC GCTGCCAGGT CAGTGTGATC TCCGCAGGGT AGAAGCCCAG GGCCCAGCAC
  481 CTCAGGGTGG CCTCATGGTC AGAGACGGGG TGGTGGGTCA CGTGTGTCTT TGGGGGGTCC
  541 GCGCGCTGCA GCGTCTCCTT CCCGTTCTCC AGGTGTCTGC GGAGCCACTC CACGCACAGG
  601 CCCTCCAGGT AGGCTCTCAG CTGCTCCGCC TCACGGGCCG CCTCCCACTT GCGCTGGGTG
  661 ATCTGAGCCG CGGTGTCCGC CGCGGTCCAG GAGCTCAGGT CCTCGTTCAG GGCGATGTAA
  721 TCTTTGCCGT CGTAGGCGTA CTGGTTATGC CCGCGGAGGA GGCGCCCGTC CGGCCCCACG
  781 TCGCAGCCAT ACATCGTCTG CCAAGTGTGA GACCCGGCCT CGCTCTGGTT GTAGTAGCGG
  841 AGCGCGATCC GCAGGTTCTC TCGGTAAGTC TGTGTGTTGG TCTTGAAGAT CTGTGTGTTC
  901 CGGTCCCAAT ACTCCCGGCC CCTCCCTGCT CTATCCATGG CGCCCGGGGG CCTCCGTCCT
  961 CGGACTCGCG GCGTCGCTGT CGAACCTCCA CGACTGGTGT CGTCCACGTT AGCCCACTGC
 1021 AATGAAGCCG GGGCTTCCCG CGGATCGGTC CGGACATTGC GATGTAAGAA AATAACCCTC
1081 CAATGGAATG
//
Primer: SV40pro_F RDB13720_A5Llc.seq
>02881_13720_A5Ll_SV40pro_F_B05_05.ab1
    1 CCCGGGCCCC TGGACATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TTCGAAATGA CCGACCAAGC
  961 GACGCCCAAC CCTGCCATCA CGAGATTTTC GATTCCCACC GCCGCCTTTC TAATGGAAGA
 1021 TTGGGGCTTC GGCATCGGTT TTCGGACGGT GGCTGGATGA ATCCTCCAGC GCGGGGGAAT
1081 TCCTCCAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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