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Human HLA-B*51:01:01 cDNA

Human HLA-B*51:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02881
Resource name Human HLA-B*51:01:01 cDNA
Alternative name HLA-B*51011
Clone info. Human HLA-B*51:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB02881 Human HLA-B*51:01:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02881z.seq
          Remarks, protocol and/or map (gif) RDB02881.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18215_B0F2p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB18215_B0F2a.seq check
          >02881_18215_B0F2_1_CMV_Forward_A07_01_ABI24.ab1
              1 NNNNNNNNNN NNNNTTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTCACCC GGACTCAGAA
            181 TCTCCTCAGA CGCCGAGATG CGGGTCACGG CGCCCCGAAC CGTCCTCCTG CTGCTCTGGG
            241 GGGCAGTGGC CCTGACCGAG ACCTGGGCCG GCTCCCACTC CATGAGGTAT TTCTACACCG
            301 CCATGTCCCG GCCCGGCCGC GGGGAGCCCC GCTTCATTGC AGTGGGCTAC GTGGACGACA
            361 CCCAGTTCGT GAGGTTCGAC AGCGACGCCG CGAGTCCGAG GACGGAGCCC CGGGCGCCAT
            421 GGATAGAGCA GGAGGGGCCG GAGTATTGGG ACCGGAACAC ACAGATCTTC AAGACCAACA
            481 CACAGACTTA CCGAGAGAAC CTGCGGATCG CGCTCCGCTA CTACAACCAG AGCGAGGCCG
            541 GGTCTCACAC TTGGCAGACG ATGTATGGCT GCGACGTGGG GCCGGACGGG CGCCTCCTCC
            601 GCGGGCATAA CCAGTACGCC TACGACGGCA AAGATTACAT CGCCCTGAAC GAGGACCTGA
            661 GCTCCTGGAC CGCGGCGGAC ACCGCGGCTC AGATCACCCA GCGCAAGTGG GAGGCGGCCC
            721 GTGNGGCGGA GCAGCTGAGA GCCTACCTGG AGGGCCTGTG CGTGGAGTGG CTCCGCAGAC
            781 ACCTGGAGAA CGGGAAGGAG ANGCTGCAGC GCGCGGACCC CCCNAAGACA CACGTGACCC
            841 ACCACCCCGT CTCTGANCAT GANGCCACCC TGANGTGCTG GGCCCTGGGC TTCNANCCTG
            901 CGGANATCAC ACNNNNCTGN CAGCGGGNTG GCGANGNNCN AACTCNNNNA CNNANCTTGN
            961 GGANNNCAGA NCAGCNNNNA NANNNNCTTC CANNNNNNGG CNNCNNNNGN NGNNGNCNTN
          1021 NNNNNNNANA NNNNNNNNNN
          //
          Primer: bGH_rev3
          Region: bGHpA,insert 3'
          Sequence file: RDB18215_B0F2b.seq check
          >02881_18215_B0F2_1_bGH_rev3_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG GGGGNNGGNN AANAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGCGGCC GCATCTCAGT
            121 CCCTCACAAG ACAGCTGTCT CAGGCTTTTC AAGCTGTGAG AGACACATCA GAGCCCTGGG
            181 CACTGTCGCT GGACGCAGCC TGAGAGTAGC TCCCTCCTTT TCCACCTGAG CTCTTCCTCC
            241 TACACATCAC AGTAGCGACC ACAGCTCCGA TGACCACAAC TGCTAGGACA GCCAGGCCAG
            301 CAACAATGCC CACGATGGGG ATGGTGGACT GGGAAGATGG CTCCCATCTC AGGGTGAGGG
            361 GCTTCGGCAG CCCCTCATGC TGTACATGGC ATGTGTATCT CTGCTCTTCT CCAGAAGGCA
            421 CCACCACAGC TGCCCACTTC TGGAAGGTTC TATCTCCTGC TGGTCTGGTC TCCACAAGCT
            481 CAGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT CAGTGTGATC TCCGCAGGGT
            541 AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC AGAGACGGGG TGGTGGGTCA
            601 CGTGTGTCTT TGGGGGGTCC GCGCGCTGCA GCGTCTCCTT CCCGTTCTCC AGGTGTCTGC
            661 GGAGCCACTC CACGCACAGG CCCTCCAGGT AGGCTCTCAG CTGCTCCGCC TCACGGGCCG
            721 CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CGGTGTCCGC CGCGGTCCAN GANCTCANGT
            781 CCTCGTTCAN GGCGATGTAA TCTTTGCCGT CNTANGCGTA NTGNTTATGC CCGCGGANGA
            841 NGCGCCCGTC CGGCCCCACG TCGCANCNNT ACATCGTCTG NCNNNTGTGA NANCCGGCCN
            901 CGCNCTGGTT GTANTANCGG ANCNCGNTNC NNNNNTCNNT CGGNAAGTCT GNGTGTNGGT
            961 CTTGNNNNNC TGNGTGTNNN NGNCCNNNNA NNNCNNNNCN NNNNNGNNNT ATCCATNNNN
          1021 NCNNGGNNNN CNNNNNNNGN NNNNNNNGNN NNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18215_B0F2c.seq check
          >02881_18215_B0F2_1_SV40pro_ori_F_E07_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGCCN CCNCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
             61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
            121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
            181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
            241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
            301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
            361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
            421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
            481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
            541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
            601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
            661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGANGAT CTCGTCGTGA CCCATGGCGA
            721 TGCCTGCTTG CCGAATATCA TGGTGGANNA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
            781 CCGGCTGGGT GTGGCGGANC GCTATCANGA NATANCGTTG GCTACCCGTG ATATTGCTGN
            841 ANANCTTGGC GGCGAANGGN CTGANCGCTT CCTCGTGCTT TACNGNATCG CCGCNCCNNA
            901 NNNNCANCGC ATCGCNNTCN ATCGCNNTCN NGNNNANNNC NNCNNANCNG GNNTNNNGGN
          961 NNCNAAATGA NNNANNNNNC GANNNCNNNN CNNNNNNTNN NNNNNNTNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl