Resource data sheet
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Human HLA-B*46:01 cDNA

Human HLA-B*46:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02880
Resource name Human HLA-B*46:01 cDNA
Alternative name HLA-B*4601
Clone info. Human HLA-B*46:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02880 Human HLA-B*46:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02880z.seq from Depositor
Electronic file Map RDB02880.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13718_A5Llp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13718_A5Lla.seq
>02880_13718_A5Ll_T7_20bp_F03_18.ab1
    1 CGTGGCCAGC GTTTAACTTA GCTTCACCCG GACTCAGAAT CTCCTCAGAC GCCGAGATGC
   61 GGGTCACGGC GCCCCGAACC GTCCTCCTGC TGCTCTCGGG AGCCCTGGCC CTGACCGAGA
  121 CCTGGGCCGG CTCCCACTCC ATGAGGTATT TCTACACCGC CATGTCCCGG CCCGGCCGCG
  181 GGGAGCCCCG CTTCATCGCA GTGGGCTACG TGGACGACAC CCAGTTCGTG AGGTTCGACA
  241 GCGACGCCGC GAGTCCGAGG ATGGCGCCCC GGGCGCCATG GATAGAGCAG GAGGGGCCGG
  301 AGTATTGGGA CCGGGAGACA CAGAAGTACA AGCGCCAGGC ACAGACTGAC CGAGTGAGCC
  361 TGCGGAACCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG GTCTCACACC CTCCAGAGGA
  421 TGTACGGCTG CGACGTGGGG CCGGACGGGC GCCTCCTCCG CGGGCATGAC CAGTCCGCCT
  481 ACGACGGCAA GGATTACATC GCCCTGAACG AGGACCTGAG CTCCTGGACC GCGGCGGACA
  541 CGGCGGCTCA GATCACCCAG CGCAAGTGGG AGGCGGCCCG TGAGGCGGAG CAGTGGAGAG
  601 CCTACCTGGA GGGCCTGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC GGGAAGGAGA
  661 CGCTGCAGCG CGCGGACCCC CCAAAGACAC ATGTGACCCA CCACCCCATC TCTGACCATG
  721 AGGCCACCCT GAGGTGCTGG GCCCTGGGCT TCTACCCTGC GGAGATCACA CTGACCTGGC
  781 AGCGGGATGG CGAGGACCAA ACTCAGGACA CCGAGCTTGT GGAGACCAGA CCAGCAGGAG
  841 ATAGAACCTT CCAGAAGTGG GCAGCTGTGG TGGTGCCTTC TGGAGAAGAG CAGAGATACA
  901 CATGCCATGT ACAGCATGAG GGGCTGCCGA AGCCCCTCAC CCTGAGATGG GAGCCATCTT
  961 CCCAGTCCAC CATCCCCATC GTGGGCATTG TTGCTGGCCC TGGCTGTCCT AGCAGTTGTG
 1021 GTCATCGGGA GCTGTGGTCG CTACTGTGAT GTGTAGAGGA GAGCTCAAGG TGGTAAAGAG
 1081 GAGCTACTCT CAGCTGCGTC CAGCGACAGT GCCAAGGGCT CTGATGTGTT CTCTCTCCAC
1141 AGGCTTGGAA AAAGTCG
//
Primer: BGH_rev RDB13718_A5Llb.seq
>02880_13718_A5Ll_BGHrev_F04_16.ab1
    1 GATCGGGGCG TCTAGACTCG AGCGGCCGCA TCTCAGTCCC TCACAAGACA GCTGTCTCAG
   61 GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC TGTCGCTGGA CGCAGCCTGA
  121 GAGTAGCTCC CTCCTTTTCC ACCTGAGCTC TTCCTCCTAC ACATCACAGT AGCGACCACA
  181 GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA CAATGCCCAC GATGGGGATG
  241 GTGGACTGGG AAGATGGCTC CCATCTCAGG GTGAGGGGCT TCGGCAGCCC CTCATGCTGT
  301 ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA CCACAGCTGC CCACTTCTGG
  361 AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCGG TGTCCTGAGT TTGGTCCTCG
  421 CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA AGCCCAGGGC CCAGCACCTC
  481 AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACAT GTGTCTTTGG GGGGTCCGCG
  541 CGCTGCAGCG TCTCCTTCCC GTTCTCCAGG TATCTGCGGA GCCACTCCAC GCACAGGCCC
  601 TCCAGGTAGG CTCTCCACTG CTCCGCCTCA CGGGCCGCCT CCCACTTGCG CTGGGTGATC
  661 TGAGCCGCCG TGTCCGCCGC GGTCCAGGAG CTCAGGTCCT CGTTCAGGGC GATGTAATCC
  721 TTGCCGTCGT AGGCGGACTG GTCATGCCCG CGGAGGAGGC GCCCGTCCGG CCCCACGTCG
  781 CAGCCGTACA TCCTCTGGAG GGTGTGAGAC CCGGGCCTCG CTCTGGGTTG TAGTAGCCGC
  841 GCAGGTTCCG CAGGCTCACT CGGGTCAGTC TGTGCCTGGC GCTGTACTTC TGTGTCTCCC
  901 GGGTCCCAAT ACTCGGCCCC TCTGCTCTAT CATGGCGCCG GGGCGCATCT CGGACTCGCG
  961 GCGTCGCTGT CGACCTCACG AACCTGGGTG TCGTTCAACG TTAGCTCACT GCGAATGAAG
1021 CGGGGCTTCC CCGCGACTGG ATCGAAGTGC GGTGTTAGTA ATA
//
Primer: SV40pro_F RDB13718_A5Llc.seq
>02880_13718_A5Ll_SV40pro_F_G04_19.ab1
    1 CGTGGTCCTT GGACATTCAG GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
  961 CGCCCAAACC TGCCCATCAC GAGATTTCGA TTTCACCCGC CGCCTTCCTA TGAAAGGTGG
 1021 GCTCGAAATC GGTTTCGGGA CGGCTGCTGA TGAATCGTCA GCGCGGGGAA TCCCTCAATG
1081 CCTTGAGT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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