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Human HLA-B*46:01 cDNA

Human HLA-B*46:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02880
Resource name Human HLA-B*46:01 cDNA
Alternative name HLA-B*4601
Clone info. Human HLA-B*46:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02880 Human HLA-B*46:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02880z.seq
Electronic file Map RDB02880.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13718_A5Llp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB13718_A5Lld.seq check
>02880_13718_B0GgF_CMV-Forward_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTNNGNCG GTANNNNNGT ACGGNGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
  181 ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCGCCCCGAA CCGTCCTCCT GCTGCTCTCG
  241 GGAGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
  301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA CGTGGACGAC
  361 ACCCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GGATGGCGCC CCGGGCGCCA
  421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGGAGA CACAGAAGTA CAAGCGCCAG
  481 GCACAGACTG ACCGAGTGAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA GAGCGAGGCC
  541 GGGTCTCACA CCCTCCAGAG GATGTACGGC TGCGACGTGG GGCCGGACGG GCGCCTCCTC
  601 CGCGGGCATG ACCAGTCCGC CTACGACGGC AAGGATTACA TCGCCCTGAA CGANGACCTG
  661 AGCTCCTGGA CCGCGGCGGA CACGGCGGCT CAGATCACCC AGCGCAAGTG GGAGGCGGCC
  721 CGTGAGGCGG ANCAGTGGAN ANCCTACCTG GANGGCCTGT GCGNGGANNG GCTCCGCAGA
  781 TACNNGGNNA ACGGGAANGA NANGCTGCAG CGCGCGNNNC CCCCCAAAGA CACATGTGAN
  841 CCNNNACCCC ATCNNNNANN NTGNNNNCNN CCCNNNNNNN CNGGGCCNNN GNCNTNNNNC
901 CNNNCNGNAN ATNNNNNNNN NN
//
Primer: BGH_rev2
Region: bGH pA, insert 3'
Sequence file: RDB13718_A5Lle.seq check
>02880_13718_B0GgF_BGH_rev2_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNNNCNAC AGNTGGCTGG CAACTAGAAG GCACAGTCGA
   61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCATCT CAGTCCCTCA
  121 CAAGACAGCT GTCTCAGGCT TTTCAAGCTG TGAGAGACAC ATCAGAGCCC TGGGCACTGT
  181 CGCTGGACGC AGCCTGAGAG TAGCTCCCTC CTTTTCCACC TGAGCTCTTC CTCCTACACA
  241 TCACAGTAGC GACCACAGCT CCGATGACCA CAACTGCTAG GACAGCCAGG CCAGCAACAA
  301 TGCCCACGAT GGGGATGGTG GACTGGGAAG ATGGCTCCCA TCTCAGGGTG AGGGGCTTCG
  361 GCAGCCCCTC ATGCTGTACA TGGCATGTGT ATCTCTGCTC TTCTCCAGAA GGCACCACCA
  421 CAGCTGCCCA CTTCTGGAAG GTTCTATCTC CTGCTGGTCT GGTCTCCACA AGCTCGGTGT
  481 CCTGAGTTTG GTCCTCGCCA TCCCGCTGCC AGGTCAGTGT GATCTCCGCA GGGTAGAAGC
  541 CCAGGGCCCA GCACCTCAGG GTGGCCTCAT GGTCAGAGAT GGGGTGGTGG GTCACATGTG
  601 TCTTTGGGGG GTCCGCGCGC TGCAGCGTCT CCTTCCCGTT CTCCAGGTAT CTGCGGANCC
  661 ACTCCACGCA CANGCCCTCC NGGTANGCTC TCCACTGCTC CGCCTCACGG GCCGCCTCCC
  721 ACTTGCGCTG GGTGATCTGA NCCGCCGNGT CCGCCGCGGT CCAGGANCTC NNNCCTCNTT
  781 CAGGGCGATG TAATCCTTGN CGTCNNANGC GGACTGNTCA TGNCCGCGGA NGANNNGCCN
  841 NNNCNNNCCC ACNTCGNANC CGNACNTNCT CNGGNNGGNN NNANANCNNG NCNNCNNTNN
901 NGNNNNANTA NNNNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40pro_ori, NeoR
Sequence file: RDB13718_A5Llf.seq check
>02880_13718_B0GgF_SV40pro_ori_F_H07_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNGC CTCTGANCTA NTCNNGAAGT AGTGAGGAGG CTTTTTTGGA
   61 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA
  121 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG
  181 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT
  241 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC
  301 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG
  361 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG
  421 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA
  481 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA
  541 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT
  601 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC
  661 AGGCTCAAGG CGCGCATGCC CGACGGCGAN GATCTCGTCG NGACCCATGG CGATGCCNGC
  721 TTGCCGAATA TCATGGTGGA AAATGGNCGC TTTTCTGGAN TCNTCGACNG NGGNCGGCTG
  781 GGNGTGGCGG ANCGCTATCN NNACNTANNG TTGGCTANCC GNGATATTGC TGAAGANCTT
  841 GGNNGNCNAA NGGGCNNANN NNTTCCNNNN GCTTTACGGN NTCNNNNNNN CNNANTNNNA
901 NNNNATCNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl