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Human HLA-B*44:03:01 cDNA

Human HLA-B*44:03:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02879
Resource name Human HLA-B*44:03:01 cDNA
Alternative name HLA-B*44031
Clone info. Human HLA-B*44:03:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB02879 Human HLA-B*44:03:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02879z.seq
          Remarks, protocol and/or map (gif) RDB02879.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15773_A8Aqp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert 5'
          Sequence file: RDB15773_A8Aqd.seq check
          >02879_15773_A8Aq_CMV-Forward_A01_01_ABI24.ab1
              1 CTGACACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
            181 ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCGCCCCGAA CCCTCCTCCT GCTGCTCTGG
            241 GGGGCAGTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
            301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCA CCGTGGGCTA CGTGGACGAC
            361 ACGCTGTTCG TGAGGTTCGA CAGCGACGCC ACGAGTCCGA GGAAGGAGCC GCGGGCGCCA
            421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGGAGA CACAGATCTC CAAGACCAAC
            481 ACACAGACTT ACCGAGAGAA CCTGCGCACC GCGCTCCGCT ACTACAACCA GAGCGAGGCC
            541 GGGTCTCACA TCATCCAGAG GATGTACGGC TGCGACGTGG GGCCGGACGG GCGCCTCCTC
            601 CGCGGGTATG ACCAGGACGC CTACGACGGC AAGGATTACA TCGCCCTGAA CGAGGACCTG
            661 AGCTCCTGGA CCGCGGCGGA CACCGCGGCT CAGATCACCC AGCGCAAGTG GGAGGCGGCC
            721 CGTGTGGCGG AGCAGCTGAG AGCCTACCTG GAGGGCCTGT GCGTGGAGTC GCTCCGCAGA
            781 TACCTGGAGA ACGGGAAGGA GACGCTGCAG CGCGCGGACC CCCCAAAGAC ACATGTGACC
            841 CACCACCCCA TCTCTGACCA TGAGGTCACC CTGAGGTGCT GGGCCCTGGG CTTCTACCCT
            901 GCGGAGATCA CACTGACCTG GCAGCGGGAT GGCGAGGACC AAACTCAGGA CACCGAGCTT
            961 GTGAGACCAG ACCAGCAGGA GATAGACCCT TTCCAGAGTG GCAGCCTGTG TTGGTGCTTC
           1021 TGGAGAGAGC CAGAGATAAC ACATGGCCCA TGTCACAGCA TGAGGACTGG TCGAAGCTCT
          1081 TTCATCCTTG AG
          //
          Primer: BGH_rev2
          Region: bGH p(A), insert 3'
          Sequence file: RDB15773_A8Aqe.seq check
          >02879_15773_A8Aq_BGH_rev2_B01_04_ABI24.ab1
              1 CATCCAGGGT AAAGGAGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
             61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCATCTCA
            121 GTCCCTCACA AGACAGCTGT CTCAGGCTTT TCAAGCTGTG AGAGACACAT CAGAGCCCTG
            181 GGCACTGTCG CTGCACGCAG CCTGAGAGTA GCTCCCTCCT TTTCCACCTG AGCTCTTCCT
            241 CCTACACATC ACAGCAGCGA CCACAGCTCC GATGACCACA ACTGCTAGGA CAGCCAGGCC
            301 AGCAACAATG CCCACGATGG GGACGGTGGA CTGGGAAGAC GGCTCCCATC TCAGGGTGAG
            361 GGGCTTCGGC AGCCCCTCAT GCTGTACATG GCATGTGTAT CTCTGCTCTT CTCCAGAAGG
            421 CACCACCACA GCTGCCCACT TCTGGAAGGT TCTATCTCCT GCTGGTCTGG TCTCCACAAG
            481 CTCGGTGTCC TGAGTTTGGT CCTCGCCATC CCGCTGCCAG GTCAGTGTGA TCTCCGCAGG
            541 GTAGAAGCCC AGGGCCCAGC ACCTCAGGGT GACCTCATGG TCAGAGATGG GGTGGTGGGT
            601 CACATGTGTC TTTGGGGGGT CCGCGCGCTG CAGCGTCTCC TTCCCGTTCT CCAGGTATCT
            661 GCGGAGCGAC TCCACGCACA GGCCCTCCAG GTAGGCTCTC AGCTGCTCCG CCACACGGGC
            721 CGCCTCCCAC TTGCGCTGGG TGATCTGAGC CGCGGTGTCC GCCGCGGTCC AGGAGCTCAG
            781 GTCCTCGTTC AGGGCGATGT AATCCTTGCC GTCGTAGGCG TCCTGGTCAT ACCCGCGGAG
            841 GAGGCGCCCG TCCGGCCCCA CGTCGCAGCC GTACATCCTC TGGATGATGT GAGACCCGGC
            901 CTCGCTCTGG TTTGTAGTAG CGGAGCGCGG TGCGCAGGTT CTCTCGGTAA GTCTGTGTGT
            961 TGGTCTTGGA GATCTGTGTC TCCCGGGTCC CAATACTCCG GCCCCTCCTG CCTCTATCCA
           1021 TGGGCGCCGC GGCTCCTTTC TCGGACTCGT TGACGTCGCT GTCGGAAACT CACGGACCAG
          1081 CCGTGTTCGT TCCAACGTAG CTCCAACGGG TGGATTGA
          //
          Primer: SV40pro_ori_F
          Region: SV40 Pro_ori, NeoR
          Sequence file: RDB15773_A8Aqf.seq check
          >02879_15773_A9L2F_SV40pro_ori_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCCNC TGCCTCTGAN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT
             61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
            121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT
            181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC
            241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
            301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC
            361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
            421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG
            481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
            541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
            601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
            661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GANGATCTCG TCGTGACCCN TGGCGATGCC
            721 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GANTCATCGA CTGTGGCCGG
            781 CTGGGTGTGG CGGANCGCTA TCANGANATA GCGTTGGCTA CCCGNGATAT TGCTGAAGAN
            841 CTTGGCGGCG AATGGGCTGA NCGCTTNCNC GNGCTTTACN GTATCGCCGC NNCNNANTCN
            901 CANCGCATCG CCNTCNATNG CNNTNNNNNN NANNTCNNNN NANCGGGNNN CNNGGNNNCN
          961 AAANGANNNA NNNNGCGNNN CCNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl