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Human HLA-B*44:03:01 cDNA

Human HLA-B*44:03:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02879
Resource name Human HLA-B*44:03:01 cDNA
Alternative name HLA-B*44031
Clone info. Human HLA-B*44:03:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02879 Human HLA-B*44:03:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02879z.seq
Electronic file Map RDB02879.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15773_A8Aqp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB15773_A8Aqd.seq check
>02879_15773_A8Aq_CMV-Forward_A01_01_ABI24.ab1
    1 CTGACACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
  181 ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCGCCCCGAA CCCTCCTCCT GCTGCTCTGG
  241 GGGGCAGTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
  301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCA CCGTGGGCTA CGTGGACGAC
  361 ACGCTGTTCG TGAGGTTCGA CAGCGACGCC ACGAGTCCGA GGAAGGAGCC GCGGGCGCCA
  421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGGAGA CACAGATCTC CAAGACCAAC
  481 ACACAGACTT ACCGAGAGAA CCTGCGCACC GCGCTCCGCT ACTACAACCA GAGCGAGGCC
  541 GGGTCTCACA TCATCCAGAG GATGTACGGC TGCGACGTGG GGCCGGACGG GCGCCTCCTC
  601 CGCGGGTATG ACCAGGACGC CTACGACGGC AAGGATTACA TCGCCCTGAA CGAGGACCTG
  661 AGCTCCTGGA CCGCGGCGGA CACCGCGGCT CAGATCACCC AGCGCAAGTG GGAGGCGGCC
  721 CGTGTGGCGG AGCAGCTGAG AGCCTACCTG GAGGGCCTGT GCGTGGAGTC GCTCCGCAGA
  781 TACCTGGAGA ACGGGAAGGA GACGCTGCAG CGCGCGGACC CCCCAAAGAC ACATGTGACC
  841 CACCACCCCA TCTCTGACCA TGAGGTCACC CTGAGGTGCT GGGCCCTGGG CTTCTACCCT
  901 GCGGAGATCA CACTGACCTG GCAGCGGGAT GGCGAGGACC AAACTCAGGA CACCGAGCTT
  961 GTGAGACCAG ACCAGCAGGA GATAGACCCT TTCCAGAGTG GCAGCCTGTG TTGGTGCTTC
 1021 TGGAGAGAGC CAGAGATAAC ACATGGCCCA TGTCACAGCA TGAGGACTGG TCGAAGCTCT
1081 TTCATCCTTG AG
//
Primer: BGH_rev2
Region: bGH p(A), insert 3'
Sequence file: RDB15773_A8Aqe.seq check
>02879_15773_A8Aq_BGH_rev2_B01_04_ABI24.ab1
    1 CATCCAGGGT AAAGGAGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCATCTCA
  121 GTCCCTCACA AGACAGCTGT CTCAGGCTTT TCAAGCTGTG AGAGACACAT CAGAGCCCTG
  181 GGCACTGTCG CTGCACGCAG CCTGAGAGTA GCTCCCTCCT TTTCCACCTG AGCTCTTCCT
  241 CCTACACATC ACAGCAGCGA CCACAGCTCC GATGACCACA ACTGCTAGGA CAGCCAGGCC
  301 AGCAACAATG CCCACGATGG GGACGGTGGA CTGGGAAGAC GGCTCCCATC TCAGGGTGAG
  361 GGGCTTCGGC AGCCCCTCAT GCTGTACATG GCATGTGTAT CTCTGCTCTT CTCCAGAAGG
  421 CACCACCACA GCTGCCCACT TCTGGAAGGT TCTATCTCCT GCTGGTCTGG TCTCCACAAG
  481 CTCGGTGTCC TGAGTTTGGT CCTCGCCATC CCGCTGCCAG GTCAGTGTGA TCTCCGCAGG
  541 GTAGAAGCCC AGGGCCCAGC ACCTCAGGGT GACCTCATGG TCAGAGATGG GGTGGTGGGT
  601 CACATGTGTC TTTGGGGGGT CCGCGCGCTG CAGCGTCTCC TTCCCGTTCT CCAGGTATCT
  661 GCGGAGCGAC TCCACGCACA GGCCCTCCAG GTAGGCTCTC AGCTGCTCCG CCACACGGGC
  721 CGCCTCCCAC TTGCGCTGGG TGATCTGAGC CGCGGTGTCC GCCGCGGTCC AGGAGCTCAG
  781 GTCCTCGTTC AGGGCGATGT AATCCTTGCC GTCGTAGGCG TCCTGGTCAT ACCCGCGGAG
  841 GAGGCGCCCG TCCGGCCCCA CGTCGCAGCC GTACATCCTC TGGATGATGT GAGACCCGGC
  901 CTCGCTCTGG TTTGTAGTAG CGGAGCGCGG TGCGCAGGTT CTCTCGGTAA GTCTGTGTGT
  961 TGGTCTTGGA GATCTGTGTC TCCCGGGTCC CAATACTCCG GCCCCTCCTG CCTCTATCCA
 1021 TGGGCGCCGC GGCTCCTTTC TCGGACTCGT TGACGTCGCT GTCGGAAACT CACGGACCAG
1081 CCGTGTTCGT TCCAACGTAG CTCCAACGGG TGGATTGA
//
Primer: SV40pro_ori_F
Region: SV40 Pro_ori, NeoR
Sequence file: RDB15773_A8Aqf.seq check
>02879_15773_A9L2F_SV40pro_ori_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCCNC TGCCTCTGAN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
  121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT
  181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC
  241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
  301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC
  361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
  421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG
  481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
  541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
  601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
  661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GANGATCTCG TCGTGACCCN TGGCGATGCC
  721 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GANTCATCGA CTGTGGCCGG
  781 CTGGGTGTGG CGGANCGCTA TCANGANATA GCGTTGGCTA CCCGNGATAT TGCTGAAGAN
  841 CTTGGCGGCG AATGGGCTGA NCGCTTNCNC GNGCTTTACN GTATCGCCGC NNCNNANTCN
  901 CANCGCATCG CCNTCNATNG CNNTNNNNNN NANNTCNNNN NANCGGGNNN CNNGGNNNCN
961 AAANGANNNA NNNNGCGNNN CCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl