Resource data sheet
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Human HLA-B*44:03:01 cDNA

Human HLA-B*44:03:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02879
Resource name Human HLA-B*44:03:01 cDNA
Alternative name HLA-B*44031
Clone info. Human HLA-B*44:03:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02879 Human HLA-B*44:03:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02879z.seq from Depositor
Electronic file Map RDB02879.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02879_A2Klp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_long RDB02879_A2Kla.seq
>02879_02879_A2Kl_T7Long_C08_08.ab1
    1 CAAGCCTGGC TAGCGTTTAA CTTAAGCTTC ACCCGGACTC AGAATCTCCT CAGACGCCGA
   61 GATGCGGGTC ACGGCGCCCC GAACCCTCCT CCTGCTGCTC TGGGGGGCAG TGGCCCTGAC
  121 CGAGACCTGG GCCGGCTCCC ACTCCATGAG GTATTTCTAC ACCGCCATGT CCCGGCCCGG
  181 CCGCGGGGAG CCCCGCTTCA TCACCGTGGG CTACGTGGAC GACACGCTGT TCGTGAGGTT
  241 CGACAGCGAC GCCACGAGTC CGAGGAAGGA GCCGCGGGCG CCATGGATAG AGCAGGAGGG
  301 GCCGGAGTAT TGGGACCGGG AGACACAGAT CTCCAAGACC AACACACAGA CTTACCGAGA
  361 GAACCTGCGC ACCGCGCTCC GCTACTACAA CCAGAGCGAG GCCGGGTCTC ACATCATCCA
  421 GAGGATGTAC GGCTGCGACG TGGGGCCGGA CGGGCGCCTC CTCCGCGGGT ATGACCAGGA
  481 CGCCTACGAC GGCAAGGATT ACATCGCCCT GAACGAGGAC CTGAGCTCCT GGACCGCGGC
  541 GGACACCGCG GCTCAGATCA CCCAGCGCAA GTGGGAGGCG GCCCGTGTGG CGGAGCAGCT
  601 GAGAGCCTAC CTGGAGGGCC TGTGCGTGGA GTCGCTCCGC AGATACCTGG AGAACGGGAA
  661 GGAGACGCTG CAGCGCGCGG ACCCCCCAAA GACACATGTG ACCCACCACC CCATCTCTGA
  721 CCATGAGGTC ACCCTGAGGT GCTGGGCCCT GGGCTTCTAC CCTGCGGAGA TCACACTGAC
  781 CTGGCAGCGG GATGGCGAGG ACCAAACTCA GGACACCGAG CTTGTGGAGA CCAGACCAGC
  841 AGGAGATAGA ACCTTCCAGA AGTGGGCAGC TGTGGTGGTG CCTTCTGGAG AAGAGCAGAG
  901 ATACACATGC CATGTACAGC ATGAGGGGCT GCCGAAGCCC CTCACCCTGA GATGGGAGCC
  961 GTCTTCCCAG TCCACCGTCC CCATCGTGGG CATTGTTGCT GGCCTGGCTG TCCTAGCAGT
 1021 TTGTGGTCAT CGGAGCTGTG GGTCGCTGCT GTGATGTGTA GGAAGGAAGA GCTCAGTGGA
 1081 AAAAGGAGGA GCTTACTCTC AGGCTGCATG CCAGCGAACA GTACCCAAGG GCTTCTTGCA
1141 TGGTGATCCT CCCTCACACA AGGCT
//
Primer: BGH_rev RDB02879_A2Klb.seq
>02879_02879_A2Kl_BGHrev_D08_11.ab1
    1 GTTTCGGACT CTAGACTCGA GCGGCCGCAT CTCAGTCCCT CACAAGACAG CTGTCTCAGG
   61 CTTTTCAAGC TGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCGCTGCAC GCAGCCTGAG
  121 AGTAGCTCCC TCCTTTTCCA CCTGAGCTCT TCCTCCTACA CATCACAGCA GCGACCACAG
  181 CTCCGATGAC CACAACTGCT AGGACAGCCA GGCCAGCAAC AATGCCCACG ATGGGGACGG
  241 TGGACTGGGA AGACGGCTCC CATCTCAGGG TGAGGGGCTT CGGCAGCCCC TCATGCTGTA
  301 CATGGCATGT GTATCTCTGC TCTTCTCCAG AAGGCACCAC CACAGCTGCC CACTTCTGGA
  361 AGGTTCTATC TCCTGCTGGT CTGGTCTCCA CAAGCTCGGT GTCCTGAGTT TGGTCCTCGC
  421 CATCCCGCTG CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC CAGCACCTCA
  481 GGGTGACCTC ATGGTCAGAG ATGGGGTGGT GGGTCACATG TGTCTTTGGG GGGTCCGCGC
  541 GCTGCAGCGT CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CGACTCCACG CACAGGCCCT
  601 CCAGGTAGGC TCTCAGCTGC TCCGCCACAC GGGCCGCCTC CCACTTGCGC TGGGTGATCT
  661 GAGCCGCGGT GTCCGCCGCG GTCCAGGAGC TCAGGTCCTC GTTCAGGGCG ATGTAATCCT
  721 TGCCGTCGTA GGCGTCCTGG TCATACCCGC GGAGGAGGCG CCCGTCCGGC CCCACGTCGC
  781 AGCCGTACAT CCTCTGGATG ATGTGAGACC CGGCCTCGCT CTGGTTGTAG TAGCGGAGCG
  841 CGGTGCGCAG GTTCTCTCGG TAAGTCTGTG TGTTGGTCTT GGAGATCTGT GTCTCCCGGT
  901 CCCAATACTC CGGCCCCTCC TGCTCTATCC ATGGCGCCCG CGGCTCCTTC CTCGGACTCG
  961 TGGCGTCGCT GTCGAACCTC ACGAACAGCG TGTCGTCCAC GTAGCCCACG GGTGATGAAG
 1021 CGGGGCTCCC CGCGGCCCGG ACCGGGACAT GGCGGGTGTA GAAAATACCC TCATGGTAGT
 1081 GGAGCCGGCC CAGGGTCTCG GGTTCAAGGA CACTGGCCCC CCAGAGCCAG GCAGAGCAGG
1141 GATTCGGGGG GCCGCCCGAT GAACCCCCGG CA
//
Primer: SV40pro_F RDB02879_A2Klc.seq
>02879_02879_A2Kl_SV40pro_F_C02_08.ab1
    1 CACGTCTGGC TCAGAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT
   61 CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG
  121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
  181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
  241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
  301 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
  361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
  421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
  481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
  541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA
  661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
  721 TGGCCGCTTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
  781 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
  841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCCTT CTATCGCCTT
  901 TCTTTGACGA GTTTCTTCTG AGCGGGACTC TGGGGGTTTC GAAATGACCC GACCCAGCGA
  961 CGCCCCAACC CTGCCAATCA CGAGATTTCG ATTCCACCGA TCGACGTCCT ATGGAATGGA
1021 TGGGCACCGT ATCGTTTCTG GACGCGGCTG GATGATTCAT CAGCGTCGGA AATCGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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