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Human HLA-B*40:02 cDNA

Human HLA-B*40:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02878
Resource name Human HLA-B*40:02 cDNA
Alternative name HLA-B*4002
Clone info. Human HLA-B*40:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          RDB02878 Human HLA-B*40:02 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02878z.seq
          Remarks, protocol and/or map (gif) RDB02878.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18239_B0Gdp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB18239_B0Gda.seq check
          >02878_18239_B0Gd_3_CMV-Forward_F08_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGCN NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTCACCCG GACTCAGAAT
            181 CTCCTCAGAC GCCGAGATGC GGGTCACGGC GCCCCGAACC CTCCTCCTGC TGCTCTGGGG
            241 GGCAGTGGCC CTGACCGAGA CCTGGGCTGG CTCCCACTCC ATGAGGTATT TCCACACCTC
            301 CGTGTCCCGG CCCGGCCGCG GGGAGCCCCG CTTCATCACC GTGGGCTACG TGGACGACAC
            361 GCTGTTCGTG AGGTTCGACA GCGACGCCAC GAGTCCGAGG AAGGAGCCGC GGGCGCCATG
            421 GATAGAGCAG GAGGGGCCGG AGTATTGGGA CCGGGAGACA CAGATCTCCA AGACCAACAC
            481 ACAGACTTAC CGAGAGAGCC TGCGGAACCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG
            541 GTCTCACACC CTCCAGAGCA TGTACGGCTG CGACGTGGGG CCGGACGGGC GCCTCCTCCG
            601 CGGGCATAAC CAGTACGCCT ACGACGGCAA GGATTACATC GCCCTGAACG AGGACCTGCG
            661 CTCCTGGACC GCCGCGGACA CGGCGGCTCA GATCACCCAG CGCAAGTGGG ANGCGGCCCG
            721 TGTGGCGGAN CAGCTGAGAG CCTACCTGGA GGGCGAGTGC GTGGAGTGGC TCCGCAGATA
            781 CCTGGANAAC GGGAAGGAGA CGCTGCAGCG CGCGGANCCC CCNAAGANAC ACGTGACCCA
            841 CCACCNCATC TCTGACCATG ANGNCNCCCN GANGTGCTGG GCCCTNGGCT NCTACCCTGC
            901 GGANNTCACA CTGNNCTGNC AGCGGGNNGG CGANGNNCAA ACTCNGGACA CTGANNTTGN
            961 GNANANCANA NCANCNNNAN ANNNANNNNT NNANANNNGG GCAGCTNNNG NNGNNGNCNN
          1021 NNNNNNNN
          //
          Primer: BGH_rev2
          Region: bGHpA,insert 3'
          Sequence file: RDB18239_B0Gdb.seq check
          >02878_18239_B0Gd_3_BGH_rev2_G08_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNNG GNGGNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGCGGCC GCATCTCAGT
            121 CCCTCACAAG ACAGCTGTCT CAGGCTTTTC AAGCTGTGAG AGACACATCA GAGCCCTGGG
            181 CACTGTCGCT GCACGCAGCC TGAGAGTAGC TCCCTCCTTT TCCACCTGAG CTCTTCCTCC
            241 TACACATCAC AGCAGCGACC ACAGCTCCGA TGACCACAAC TGCTAGGACA GCCAGGCCAG
            301 CAACAATGCC CACGATGGGG ACGGTGGACT GGGAAGACGG CTCCCATCTC AGGGTGAGGG
            361 GCTTCGGCAG CCCCTCATGC TGTACATGGC ATGTGTATCT CTGCTCTTCT CCAGAAGGCA
            421 CCACCACAGC TGCCCACTTC TGGAAGGTTC TATCTCCTGC TGGTCTGGTC TCCACAAGCT
            481 CAGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT CAGTGTGATC TCCGCAGGGT
            541 AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC AGAGATGGGG TGGTGGGTCA
            601 CGTGTGTCTT TGGGGGGTCC GCGCGCTGCA GCGTCTCCTT CCCGTTCTCC AGGTATCTGC
            661 GGAGCCACTC CACGCACTCG CCCTCCAGGT AGGCTCTCAG CTGCTCCGCC ACACGGGCCG
            721 CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CCGTGTCCGC GGCGGTCCAG GANCGCANGT
            781 CCTCGTTCAN GGCGATGTAA TCCTTGCCGT CGTANGCGTA CTGGTTATGC CCGCGGANGA
            841 NGCGCCCGTC CGGCNNNACG TCGCANCCGT ACATGCTCTG GANGGTGTGA GANCCGGCCT
            901 CGCTCTGGTT GTAGTANCCG CGCANGTTNC GCANGNTCTC NCNNTAANTC TGTGTGNTGG
            961 NCTTGGANNN NTGTGTNNNC CGGNCCNNNN ANTCCGGNCC NNNCNGNNNT ATCNATGGNN
          1021 NCNNNNGNNN NNTNNNNNNG NNNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18239_B0Gdc.seq check
          >02878_18239_B0Gd_3_SV40pro_ori_F_H08_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
             61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
            121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
            181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
            241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
            301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
            361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
            421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
            481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
            541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
            601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
            661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGATCTC GTCGTGANCC ATGGCGATGC
            721 CTGCTTGCCG AATATCATGG TGNNNNNGGC CGCTTTTCTG GANTCATCGA CTGTGGCCGG
            781 CTGGGTGTGG CGGANNGCTA TCAGGANATA GCGTTGGCTA NCCGTGANAN TGCTGNANAN
            841 CTTGGCGGCG AATGGGCTGA NCGCTTCCTC NNGCTTTACG GTATCGCCGC NCCCGATTCN
            901 CANCGCATCG CCNNCNATCG CNNTNNNNAC NANNNCNNCN GANCGGNNNT NNGGGGNTNN
          961 NAANNGANNN ANNNNNCNAN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl