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Human HLA-B*40:02 cDNA

Human HLA-B*40:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02878
Resource name Human HLA-B*40:02 cDNA
Alternative name HLA-B*4002
Clone info. Human HLA-B*40:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02878 Human HLA-B*40:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02878z.seq
Electronic file Map RDB02878.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18239_B0Gdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,insert 5'
Sequence file: RDB18239_B0Gda.seq check
>02878_18239_B0Gd_3_CMV-Forward_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCN NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTCACCCG GACTCAGAAT
  181 CTCCTCAGAC GCCGAGATGC GGGTCACGGC GCCCCGAACC CTCCTCCTGC TGCTCTGGGG
  241 GGCAGTGGCC CTGACCGAGA CCTGGGCTGG CTCCCACTCC ATGAGGTATT TCCACACCTC
  301 CGTGTCCCGG CCCGGCCGCG GGGAGCCCCG CTTCATCACC GTGGGCTACG TGGACGACAC
  361 GCTGTTCGTG AGGTTCGACA GCGACGCCAC GAGTCCGAGG AAGGAGCCGC GGGCGCCATG
  421 GATAGAGCAG GAGGGGCCGG AGTATTGGGA CCGGGAGACA CAGATCTCCA AGACCAACAC
  481 ACAGACTTAC CGAGAGAGCC TGCGGAACCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG
  541 GTCTCACACC CTCCAGAGCA TGTACGGCTG CGACGTGGGG CCGGACGGGC GCCTCCTCCG
  601 CGGGCATAAC CAGTACGCCT ACGACGGCAA GGATTACATC GCCCTGAACG AGGACCTGCG
  661 CTCCTGGACC GCCGCGGACA CGGCGGCTCA GATCACCCAG CGCAAGTGGG ANGCGGCCCG
  721 TGTGGCGGAN CAGCTGAGAG CCTACCTGGA GGGCGAGTGC GTGGAGTGGC TCCGCAGATA
  781 CCTGGANAAC GGGAAGGAGA CGCTGCAGCG CGCGGANCCC CCNAAGANAC ACGTGACCCA
  841 CCACCNCATC TCTGACCATG ANGNCNCCCN GANGTGCTGG GCCCTNGGCT NCTACCCTGC
  901 GGANNTCACA CTGNNCTGNC AGCGGGNNGG CGANGNNCAA ACTCNGGACA CTGANNTTGN
  961 GNANANCANA NCANCNNNAN ANNNANNNNT NNANANNNGG GCAGCTNNNG NNGNNGNCNN
1021 NNNNNNNN
//
Primer: BGH_rev2
Region: bGHpA,insert 3'
Sequence file: RDB18239_B0Gdb.seq check
>02878_18239_B0Gd_3_BGH_rev2_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNG GNGGNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGCGGCC GCATCTCAGT
  121 CCCTCACAAG ACAGCTGTCT CAGGCTTTTC AAGCTGTGAG AGACACATCA GAGCCCTGGG
  181 CACTGTCGCT GCACGCAGCC TGAGAGTAGC TCCCTCCTTT TCCACCTGAG CTCTTCCTCC
  241 TACACATCAC AGCAGCGACC ACAGCTCCGA TGACCACAAC TGCTAGGACA GCCAGGCCAG
  301 CAACAATGCC CACGATGGGG ACGGTGGACT GGGAAGACGG CTCCCATCTC AGGGTGAGGG
  361 GCTTCGGCAG CCCCTCATGC TGTACATGGC ATGTGTATCT CTGCTCTTCT CCAGAAGGCA
  421 CCACCACAGC TGCCCACTTC TGGAAGGTTC TATCTCCTGC TGGTCTGGTC TCCACAAGCT
  481 CAGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT CAGTGTGATC TCCGCAGGGT
  541 AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC AGAGATGGGG TGGTGGGTCA
  601 CGTGTGTCTT TGGGGGGTCC GCGCGCTGCA GCGTCTCCTT CCCGTTCTCC AGGTATCTGC
  661 GGAGCCACTC CACGCACTCG CCCTCCAGGT AGGCTCTCAG CTGCTCCGCC ACACGGGCCG
  721 CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CCGTGTCCGC GGCGGTCCAG GANCGCANGT
  781 CCTCGTTCAN GGCGATGTAA TCCTTGCCGT CGTANGCGTA CTGGTTATGC CCGCGGANGA
  841 NGCGCCCGTC CGGCNNNACG TCGCANCCGT ACATGCTCTG GANGGTGTGA GANCCGGCCT
  901 CGCTCTGGTT GTAGTANCCG CGCANGTTNC GCANGNTCTC NCNNTAANTC TGTGTGNTGG
  961 NCTTGGANNN NTGTGTNNNC CGGNCCNNNN ANTCCGGNCC NNNCNGNNNT ATCNATGGNN
1021 NCNNNNGNNN NNTNNNNNNG NNNNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB18239_B0Gdc.seq check
>02878_18239_B0Gd_3_SV40pro_ori_F_H08_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
  121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
  541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
  601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
  661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGATCTC GTCGTGANCC ATGGCGATGC
  721 CTGCTTGCCG AATATCATGG TGNNNNNGGC CGCTTTTCTG GANTCATCGA CTGTGGCCGG
  781 CTGGGTGTGG CGGANNGCTA TCAGGANATA GCGTTGGCTA NCCGTGANAN TGCTGNANAN
  841 CTTGGCGGCG AATGGGCTGA NCGCTTCCTC NNGCTTTACG GTATCGCCGC NCCCGATTCN
  901 CANCGCATCG CCNNCNATCG CNNTNNNNAC NANNNCNNCN GANCGGNNNT NNGGGGNTNN
961 NAANNGANNN ANNNNNCNAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl