Resource data sheet
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Human HLA-B*40:02 cDNA

Human HLA-B*40:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02878
Resource name Human HLA-B*40:02 cDNA
Alternative name HLA-B*4002
Clone info. Human HLA-B*40:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02878 Human HLA-B*40:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02878z.seq from Depositor
Electronic file Map RDB02878.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02878_A2Klp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_long RDB02878_A2Kla.seq
>02878_02878_A2Kl_T7Long_A08_02.ab1
    1 CAAGCTGGCT AGCGTTTAAC TTAAGCTTCA CCCGGACTCA GAATCTCCTC AGACGCCGAG
   61 ATGCGGGTCA CGGCGCCCCG AACCCTCCTC CTGCTGCTCT GGGGGGCAGT GGCCCTGACC
  121 GAGACCTGGG CTGGCTCCCA CTCCATGAGG TATTTCCACA CCTCCGTGTC CCGGCCCGGC
  181 CGCGGGGAGC CCCGCTTCAT CACCGTGGGC TACGTGGACG ACACGCTGTT CGTGAGGTTC
  241 GACAGCGACG CCACGAGTCC GAGGAAGGAG CCGCGGGCGC CATGGATAGA GCAGGAGGGG
  301 CCGGAGTATT GGGACCGGGA GACACAGATC TCCAAGACCA ACACACAGAC TTACCGAGAG
  361 AGCCTGCGGA ACCTGCGCGG CTACTACAAC CAGAGCGAGG CCGGGTCTCA CACCCTCCAG
  421 AGCATGTACG GCTGCGACGT GGGGCCGGAC GGGCGCCTCC TCCGCGGGCA TAACCAGTAC
  481 GCCTACGACG GCAAGGATTA CATCGCCCTG AACGAGGACC TGCGCTCCTG GACCGCCGCG
  541 GACACGGCGG CTCAGATCAC CCAGCGCAAG TGGGAGGCGG CCCGTGTGGC GGAGCAGCTG
  601 AGAGCCTACC TGGAGGGCGA GTGCGTGGAG TGGCTCCGCA GATACCTGGA GAACGGGAAG
  661 GAGACGCTGC AGCGCGCGGA CCCCCCAAAG ACACACGTGA CCCACCACCC CATCTCTGAC
  721 CATGAGGCCA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGCGGAGAT CACACTGACC
  781 TGGCAGCGGG ATGGCGAGGA CCAAACTCAG GACACTGAGC TTGTGGAGAC CAGACCAGCA
  841 GGAGATAGAA CCTTCCAGAA GTGGGCAGCT GTGGTGGTGC CTTCTGGAGA AGAGCAGAGA
  901 TACACATGCC ATGTACAGCA TGAGGGGCTG CCGAAGCCCC TCACCCTGAG ATGGGAGCCG
  961 TCTTCCCAGT CCACCGTCCC CATCGTGGGC ATTGTTGCTG GCCTGGCTGT CCTAGCAGTT
 1021 TGTGGTCATC GGGAGCTGTG GGTCGCTGCT GTGATGTGTA AGAGGAAGAG CTCAGTGGAA
 1081 AAAGGAGGGA GCTACTCTCA GGCCTGCCAT GCAGCGGACA GTACCCAGGG CCTTCTTGCA
1141 TGGGTGTTCT CCTTCA
//
Primer: BGH_rev RDB02878_A2Klb.seq
>02878_02878_A2Kl_BGHrev_B08_05.ab1
    1 GGTTCGGGAC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC TCACAAGACA GCTGTCTCAG
   61 GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC TGTCGCTGCA CGCAGCCTGA
  121 GAGTAGCTCC CTCCTTTTCC ACCTGAGCTC TTCCTCCTAC ACATCACAGC AGCGACCACA
  181 GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA CAATGCCCAC GATGGGGACG
  241 GTGGACTGGG AAGACGGCTC CCATCTCAGG GTGAGGGGCT TCGGCAGCCC CTCATGCTGT
  301 ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA CCACAGCTGC CCACTTCTGG
  361 AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG TGTCCTGAGT TTGGTCCTCG
  421 CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA AGCCCAGGGC CCAGCACCTC
  481 AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACGT GTGTCTTTGG GGGGTCCGCG
  541 CGCTGCAGCG TCTCCTTCCC GTTCTCCAGG TATCTGCGGA GCCACTCCAC GCACTCGCCC
  601 TCCAGGTAGG CTCTCAGCTG CTCCGCCACA CGGGCCGCCT CCCACTTGCG CTGGGTGATC
  661 TGAGCCGCCG TGTCCGCGGC GGTCCAGGAG CGCAGGTCCT CGTTCAGGGC GATGTAATCC
  721 TTGCCGTCGT AGGCGTACTG GTTATGCCCG CGGAGGAGGC GCCCGTCCGG CCCCACGTCG
  781 CAGCCGTACA TGCTCTGGAG GGTGTGAGAC CCGGCCTCGC TCTGGTTGTA GTAGCCGCGC
  841 AGGTTCCGCA GGCTCTCTCG GTAAGTCTGT GTGTTGGTCT TGGAGATCTG TGTCTCCCGG
  901 TCCCAATACT CCGGCCCCTC CTGCTCTATC CATGGCGCCC GCGGCTCCTT CCTCGGACTC
  961 GTGGCGTCGC TGTCGAACCT CACGAAACAG CGTGTCGTCC ACGTAGCCCA CGGGTGATGA
 1021 AGCGGGGCTC CCCGCGGGCC CGGGGCCGGG ACACGGAGGT GTGGAAATAC CTCATGTAGT
 1081 GGGGAGCCAG CCCAGGGTCT CGTCAGACAA CTGACCCCCC AGAGCCAGCC AGAGGCATGG
1141 ATTTCCGGGG GCGCCCAT
//
Primer: SV40pro_F RDB02878_A2Klc.seq
>02878_02878_A2Kl_SV40pro_F_B02_05.ab1
    1 CACGGTCTGG ACATCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCTT
  901 CTTGACGAGT TCTTCTGAGC GGACTCTGGG GGTTCGAAAT GACGACAGCG ACGCCCATCT
  961 TGCATCACGA GATTCGATCC AACGCGCTTC TATGGAACGT GGCTCGGATC GTTCAGGGAA
1021 CGCTGACTGA TGATCTCAGC CGGGATCTCA TGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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