Resource data sheet
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Human HLA-B*40:01:02 cDNA

Human HLA-B*40:01:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02877
Resource name Human HLA-B*40:01:02 cDNA
Alternative name HLA-B*40012
Clone info. Human HLA-B*40:01:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02877 Human HLA-B*40:01:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02877z.seq from Depositor
Electronic file Map RDB02877.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02877_A3G8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB02877_A3G8a.seq
>02877_02877_A3G8_T7_20bp_A06_03.ab1
    1 CGTTACAGCC GCTTACTTAA GCTTCACCCG GACTCAGAAT CTCCTCAGAC GCCGAGATGC
   61 GGGTCACGGC ACCCCGAACC GTCCTCCTGC TGCTCTCGGC GGCCCTGGCC CTGACCGAGA
  121 CCTGGGCCGG CTCCCACTCC ATGAGGTATT TCCACACCGC CATGTCCCGG CCCGGCCGCG
  181 GGGAGCCCCG CTTCATCACC GTGGGCTACG TGGACGACAC GCTGTTCGTG AGGTTCGACA
  241 GCGACGCCAC GAGTCCGAGG AAGGAGCCGC GGGCGCCATG GATAGAGCAG GAGGGGCCGG
  301 AGTATTGGGA CCGGGAGACA CAGATCTCCA AGACCAACAC ACAGACTTAC CGAGAGAGCC
  361 TGCGGAACCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG GTCTCACACC CTCCAGAGGA
  421 TGTACGGCTG CGACGTGGGG CCGGACGGGC GCCTCCTCCG CGGGCATAAC CAGTACGCCT
  481 ACGACGGCAA GGATTACATC GCCCTGAACG AGGACCTGCG CTCCTGGACC GCCGCGGACA
  541 CGGCGGCTCA GATCTCCCAG CGCAAGTTGG AGGCGGCCCG TGTGGCGGAG CAGCTGAGAG
  601 CCTACCTGGA GGGCGAGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC GGGAAGGACA
  661 AGCTGGAGCG CGCTGACCCC CCAAAGACAC ACGTGACCCA CCACCCCATC TCTGACCATG
  721 AGGCCACCCT GAGGTGCTGG GCCCTGGGTT TCTACCCTGC GGAGATCACA CTGACCTGGC
  781 AGCGGGATGG CGAGGACCAA ACTCAGGACA CTGAGCTTGT GGAGACCAGA CCAGCAGGAG
  841 ATAGAACCTT CCAGAAGTGG GCAGCTGTGG TGCTGCCTTC TGGAGAAGAG CAGAGATACA
  901 CATGCCATGT ACAGCATGAG GGGCTGCCGA AGCCCCTCAC CCTGAGATGG GAGCCGTCTT
  961 CCCAGTCCAC CGTCCCCATC GTGGGCATTG TTGCTGGCCT GGCTGTCCTA GCAGTTGTGG
 1021 TCATCGGGAG CTGTGGTCGC TGCTGTGATG TGTAGGAGGA AGAGTTCAAG TGGAAAAAGG
 1081 AGGGAGCTAC TCTCAAGCTG CGTGCAAGCG GACAGTGCCA GGCTCTGGAT GTGTTCTCTC
1141 ACAGCTTGCA AAAAGCCTGG AAAAAACAGG CC
//
Primer: BGH_rev RDB02877_A3G8b.seq
>02877_02877_A3G8_BGHrev_B06_06.ab1
    1 CATACGGGAC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC TCACAAGACA GCTGTCTCAG
   61 GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC TGTCGCTGCA CGCAGCCTGA
  121 GAGTAGCTCC CTCCTTTTCC ACCTGAACTC TTCCTCCTAC ACATCACAGC AGCGACCACA
  181 GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA CAATGCCCAC GATGGGGACG
  241 GTGGACTGGG AAGACGGCTC CCATCTCAGG GTGAGGGGCT TCGGCAGCCC CTCATGCTGT
  301 ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA CCACAGCTGC CCACTTCTGG
  361 AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG TGTCCTGAGT TTGGTCCTCG
  421 CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA AACCCAGGGC CCAGCACCTC
  481 AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACGT GTGTCTTTGG GGGGTCAGCG
  541 CGCTCCAGCT TGTCCTTCCC GTTCTCCAGG TATCTGCGGA GCCACTCCAC GCACTCGCCC
  601 TCCAGGTAGG CTCTCAGCTG CTCCGCCACA CGGGCCGCCT CCAACTTGCG CTGGGAGATC
  661 TGAGCCGCCG TGTCCGCGGC GGTCCAGGAG CGCAGGTCCT CGTTCAGGGC GATGTAATCC
  721 TTGCCGTCGT AGGCGTACTG GTTATGCCCG CGGAGGAGGC GCCCGTCCGG CCCCACGTCG
  781 CAGCCGTACA TCCTCTGGAG GGTGTGAGAC CCGGCCTCGC TCTGGTTGTA GTAGCCGCGC
  841 AGGTTCCGCA GGCTCTCTCG GTAAGTCTGT GTGTTGGTCT TGGAGATCTG TGTCTCCCGG
  901 TCCCAATACT CCGGCCCCTC CTGCTCTATC CATGGCGCCC GCGGCTCCTT CCTCGGACTC
  961 GTGGCGTCGC TGTCGAACCT CACGAACAGC GTGTCGTCCA CGTAGCCCAC GGGTGATGAA
 1021 GCGGGGCTCC CCGCGGGCCG GGCCGGGACA TGGCGGTGTG CAAATACCCT CATGGTAGTG
 1081 GAGTGACCAG TCTCGGATCA TGACAGGACG CGAGAGCCAG CCAGAAGACG GATTCGGGGG
1141 TGGCCGATTA CCCCCGCA
//
Primer: SV40pro_F RDB02877_A3G8c.seq
>02877_02877_A3G8_SV40pro_F_A02_02.ab1
    1 CTTGTCTTGA CATCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
  121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
  661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGTGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCC TTCTATCGCC
  901 TTTCTTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGGTTT CGAAATGACC CGACCCAGCG
  961 ACGCCCAAAC CTGCCAATCA ACGAGATTTC GATTCCACCG CCGGCGTCCT ATGGAAAGGT
 1021 TGGGGCTTCC GGATTCGTTT CAGAACGCTG ACTGCATGAT TCCTTCCAGC GTCGGGAACT
1081 CTCTCCATG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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