Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-B*40:01:02 cDNA

Human HLA-B*40:01:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02877
Resource name Human HLA-B*40:01:02 cDNA
Alternative name HLA-B*40012
Clone info. Human HLA-B*40:01:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02877 Human HLA-B*40:01:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02877z.seq
Electronic file Map RDB02877.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13916_A6Aqp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB13916_A6Aqc.seq check
>02877_13916_B0GgF_CMV-Forward_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTGGGCG GTANGCGTGT ACGGTGGGAN GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
  181 ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCACCCCGAA CCGTCCTCCT GCTGCTCTCG
  241 GCGGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCCACACC
  301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCA CCGTGGGCTA CGTGGACGAC
  361 ACGCTGTTCG TGAGGTTCGA CAGCGACGCC ACGAGTCCGA GGAAGGAGCC GCGGGCGCCA
  421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGGAGA CACAGATCTC CAAGACCAAC
  481 ACACAGACTT ACCGAGNNAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA GAGCGAGGCC
  541 GGGTCTCACA CCCTCCAGAG GATGTACGGC TGCGACGTGG GGCCGGACGG GCGCCTCCTC
  601 CGCGGGCATA ACCAGTACGC CTACGACGGC NAGGNNTACA TCGCCCTGAA CGANGNNCTG
  661 CGCTCCTGGA CCGCCGCGGA CACGGCGGCT CANATCTCCC AGCGCANGTT GGANGCGGCC
  721 CGTGTGGCGG ANCAGCTGAN NNCCTACCTG GNNGGCGANT GCGNGGNNNG GCTCCNCAGA
  781 TACCTGNANA ACGGGAANGN NAAGCTNGNN CGCGCNNACC CCCCCNAAGN NNCNCNNNNC
  841 CNNCCNCCCC NNTCNNNNAN NNNNNNNCCN CCNNNNNNNN CNGGNNCNNN GNNTNNNNNN
901 NNNN
//
Primer: BGH_rev2
Region: bGH pA, insert 3'
Sequence file: RDB13916_A6Aqd.seq check
>02877_13916_B0GgF_BGH_rev2_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNGGNGGNN GGGCNNNCAA CAGNTGGCTG GCAACTAGAA GGCACAGTCG
   61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCATC TCAGTCCCTC
  121 ACAAGACAGC TGTCTCAGGC TTTTCAAGCT GTGAGAGACA CATCAGAGCC CTGGGCACTG
  181 TCGCTGCACG CAGCCTGAGA GTAGCTCCCT CCTTTTCCAC CTGAACTCTT CCTCCTACAC
  241 ATCACAGCAG CGACCACAGC TCCGATGACC ACAACTGCTA GGACAGCCAG GCCAGCAACA
  301 ATGCCCACGA TGGGGACGGT GGACTGGGAA GACGGCTCCC ATCTCAGGGT GAGGGGCTTC
  361 GGCAGCCCCT CATGCTGTAC ATGGCATGTG TATCTCTGCT CTTCTCCAGA AGGCACCACC
  421 ACAGCTGCCC ACTTCTGGAA GGTTCTATCT CCTGCTGGTC TGGTCTCCAC AAGCTCAGTG
  481 TCCTGAGTTT GGTCCTCGCC ATCCCGCTGC CAGGTCAGTG TGATCTCCGC AGGGTAGAAA
  541 CCCAGGGCCC AGCACCTCAN GGTGGCCTCA TGGTCAGAGA TGGGGTGGTG GGTCACGTGT
  601 GTCTTTGGGG GGTCAGCGCG CTCCAGCTTG TCCTTCCCGT TCTCCAGGTA TCTGCGGANC
  661 CACTCCACGC ACTCGCCCTC CAGGTANGCT CTCANCTGCT CCGCCACACG GGCCGCCTCC
  721 NACTTGCGCT GGGNNANCTG ANCCGCCGTG TNCGCGGCGG TCCANGANCG CNNNCCTCGT
  781 TCNNGGCGAT GTAATCCTTG CCGTCNNNNN GTACNGGTNN TGCCCGCGGA NGANGNGCCN
  841 NNNNNGNCCC NCNNCNCANN NNNACNTCCN NNNNNNGGNN NNANANCCNG NCNTCNNNNN
901 NGNNNNNNNN NN
//
Primer: SV40pro_F
Region: SV40 peo_ori, NeoR
Sequence file: RDB13916_A6Aqe.seq check
>02877_13916_B0GgF_SV40pro_ori_F_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNCCGCCNC TGCCTCTGAN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
  121 CAAGAGACAG GATGAGGNTC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT
  181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC
  241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
  301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC
  361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
  421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG
  481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
  541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
  601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
  661 GCCAGGCTCN AGGCGCGCAT GCCCGACGGC GANGNTCTCG TCGTGACCCA TGGCGATGCC
  721 TGCTTGCCGA ATATCATGGN GGAAANTGGN CGCTTTNCTG GANTCATCNN CNGNGGCCGG
  781 CNGGGNGTGG CNGNNCGCTN TCNNNANNTA NCGTTNGCTA NNNNNNANNT TGCNGNNNNN
841 CTNGNNGGNN NANNGGGNNN NNNGNNTNCN NCNNNNNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl