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Human HLA-B*35:01:01 cDNA

Human HLA-B*35:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02876
Resource name Human HLA-B*35:01:01 cDNA
Alternative name HLA-B*35011
Clone info. Human HLA-B*35:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          RDB02876 Human HLA-B*35:01:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02876z.seq
          Remarks, protocol and/or map (gif) RDB02876.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13692_A5Jgp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert5'
          Sequence file: RDB13692_A5Jga.seq check
          >02876_13692_A5Jg_CMV-Forward_E05_14.ab1
              1 CGTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
            181 ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCGCCCCGAA CCGTCCTCCT GCTGCTCTGG
            241 GGGGCAGTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
            301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA CGTGGACGAC
            361 ACCCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GGACGGAGCC CCGGGCGCCA
            421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGAACA CACAGATCTT CAAGACCAAC
            481 ACACAGACTT ACCGAGAGAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA GAGCGAGGCC
            541 GGGTCTCACA TCATCCAGAG GATGTATGGC TGCGACCTGG GGCCCGACGG GCGCCTCCTC
            601 CGCGGGCATG ACCAGTCCGC CTACGACGGC AAGGATTACA TCGCCCTGAA CGAGGACCTG
            661 AGCTCCTGGA CCGCGGCGGA CACCGCGGCT CAGATCACCC AGCGCAAGTG GGAGGCGGCC
            721 CGTGTGGCGG AGCAGCTGAG AGCCTACCTG GAGGGCCTGT GCGTGGAGTG GCTCCGCAGA
            781 TACCTGGAGA ACGGGAAGGA GACGCTGCAG CGCGCGGACC CCCCAAAGAC ACACGTGACC
            841 CACCACCCCG TCTCTGACCA TGAGGCCACC CTGAGGTGCT GGGCCCTGGG CTTCTACCCT
            901 GCGGAGATCA CACTGACCTG GCAGCGGGAT GGCGAGGACC AAACTCAGGA CACTGAGCTT
            961 GTGGAGACCA GACCAGCAGA GATAGAAACC TTCCAGAAGT GGGCAGCTGT GGTGGTGCCT
           1021 TCTGGGAGAA AGAGCAGAGA TACACATGCC CATGTACAGC ATTGAGGGGC TGCCCGAAGC
           1081 CCCTCACCCT TGGAGATTGG GAGCCATCCT TCCCAGTCCA CCATCCCCAA ATTCGGTGGG
          1141 GCCAA
          //
          Primer: BGH_rev2
          Region: bGHp(A),insert3'
          Sequence file: RDB13692_A5Jgd.seq check
          >02876_13692_B0A7F_BGH_rev2_A03_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNNGGNNNNN NNACAGATGG CTGGCAACTA GAAGGCACAG
             61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC ATCTCAGTCC
            121 CTCACAAGAC AGCTGTCTCA GGCTTTTCAA GCTGTGAGAG ACACATCAGA GCCCTGGGCA
            181 CTGTCGCTGG ACGCAGCCTG AGAGTAGCTC CCTCCTTTTC CACCTGAGCT CTTCCTCCTA
            241 CACATCACAG TAGCGACCAC AGCTCCGATG ACCACAACTG CTAGGACAGC CAGGCCAGCA
            301 ACAATGCCCA CGATGGGGAT GGTGGACTGG GAAGATGGCT CCCATCTCAG GGTGAGGGGC
            361 TTCGGCAGCC CCTCATGCTG TACATGGCAT GTGTATCTCT GCTCTTCTCC AGAAGGCACC
            421 ACCACAGCTG CCCACTTCTG GAAGGTTCTA TCTCCTGCTG GTCTGGTCTC CACAAGCTCA
            481 GTGTCCTGAG TTTGGTCCTC GCCATCCCGC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG
            541 AAGCCCAGGG CCCAGCACCT CAGGGTGGCC TCATGGTCAG AGACGGGGTG GTGGGTCACG
            601 TGTGTCTTTG GGGGGTCCGC GCGCTGCAGC GTCTCCTTCC CGTTCTCCAG GTATCTGCGG
            661 AGCCACTCCA CGCACAGGCC CTCCAGGTAG GCTCTCAGCT GCTCCGCCAC ACGGGCCGCC
            721 TCCCACTTGC GCTGGGTGAT CTGANCCGCG GTGTCCGCCG CGGTCCAGGA GCTCANGTCC
            781 TCGTTCAGGG CGATGTAATC CTTGCCGTCG TANGCGGANT GGTCATGCCC GCGGANGANG
            841 CGCCNGTCNG GCCCCANGNC GCAGCCNTAC NTCCTCNNGA TGANGTGANA NCNGGCCTCG
            901 CTCTGGTTGT ANTANCCGCG CANGNNNCGC NNNNTCTCTC NNNNAGTCTG NGTGNTGGTC
          961 TTNNNNATCN NNGNNNTNCG GTCCNNNNNN TNCNGNCNNN NNNNNNN
          //
          Primer: SV40pro_F_V2
          Region: NeoR
          Sequence file: RDB13692_A5Jgc.seq check
          >02876_13692_A5Jg_SV40pro_F_V2_G05_20.ab1
              1 GTAAGAACTT TTTGGAGGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGT ATATCCATTT
             61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
            121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
            181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
            241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
            301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
            361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
            421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
            481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
            541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
            601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
            661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
            721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
            781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
            841 CTCCCGATTC GCAGCGCATC GGCCTTCTAT CGGCCTTCTT GACGAGTTTC TTCTGAGCGG
            901 GACTCTGGGG GTTTCGAAAT GAACCGACCA AGCGGACGGC CCAACCCTGC CATCACGAGA
            961 TTTTCGAATT CCACCCGCCC GCCCTTCTAT GGAAAGGGTT GGGGCTTTCG GGAATCGGTT
           1021 TTTCCCGGGG ACGGCCCGGC CTGGGATGAA TCCTTCCCAG CGCGGGGGGG ATACTCATGC
          1081 CTGGGGAAGG TTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl