Resource data sheet
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Human HLA-B*35:01:01 cDNA

Human HLA-B*35:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02876
Resource name Human HLA-B*35:01:01 cDNA
Alternative name HLA-B*35011
Clone info. Human HLA-B*35:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02876 Human HLA-B*35:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02876z.seq from Depositor
Electronic file Map RDB02876.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13692_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13692_A5Jga.seq
>02876_13692_A5Jg_CMV-Forward_E05_14.ab1
    1 CGTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
  181 ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCGCCCCGAA CCGTCCTCCT GCTGCTCTGG
  241 GGGGCAGTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
  301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA CGTGGACGAC
  361 ACCCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GGACGGAGCC CCGGGCGCCA
  421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGAACA CACAGATCTT CAAGACCAAC
  481 ACACAGACTT ACCGAGAGAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA GAGCGAGGCC
  541 GGGTCTCACA TCATCCAGAG GATGTATGGC TGCGACCTGG GGCCCGACGG GCGCCTCCTC
  601 CGCGGGCATG ACCAGTCCGC CTACGACGGC AAGGATTACA TCGCCCTGAA CGAGGACCTG
  661 AGCTCCTGGA CCGCGGCGGA CACCGCGGCT CAGATCACCC AGCGCAAGTG GGAGGCGGCC
  721 CGTGTGGCGG AGCAGCTGAG AGCCTACCTG GAGGGCCTGT GCGTGGAGTG GCTCCGCAGA
  781 TACCTGGAGA ACGGGAAGGA GACGCTGCAG CGCGCGGACC CCCCAAAGAC ACACGTGACC
  841 CACCACCCCG TCTCTGACCA TGAGGCCACC CTGAGGTGCT GGGCCCTGGG CTTCTACCCT
  901 GCGGAGATCA CACTGACCTG GCAGCGGGAT GGCGAGGACC AAACTCAGGA CACTGAGCTT
  961 GTGGAGACCA GACCAGCAGA GATAGAAACC TTCCAGAAGT GGGCAGCTGT GGTGGTGCCT
 1021 TCTGGGAGAA AGAGCAGAGA TACACATGCC CATGTACAGC ATTGAGGGGC TGCCCGAAGC
 1081 CCCTCACCCT TGGAGATTGG GAGCCATCCT TCCCAGTCCA CCATCCCCAA ATTCGGTGGG
1141 GCCAA
//
Primer: BGH_rev RDB13692_A5Jgb.seq
>02876_13692_A5Jg_BGHrev_F05_17.ab1
    1 GATTCGGGAC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC TCACAAGACA GCTGTCTCAG
   61 GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC TGTCGCTGGA CGCAGCCTGA
  121 GAGTAGCTCC CTCCTTTTCC ACCTGAGCTC TTCCTCCTAC ACATCACAGT AGCGACCACA
  181 GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA CAATGCCCAC GATGGGGATG
  241 GTGGACTGGG AAGATGGCTC CCATCTCAGG GTGAGGGGCT TCGGCAGCCC CTCATGCTGT
  301 ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA CCACAGCTGC CCACTTCTGG
  361 AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG TGTCCTGAGT TTGGTCCTCG
  421 CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA AGCCCAGGGC CCAGCACCTC
  481 AGGGTGGCCT CATGGTCAGA GACGGGGTGG TGGGTCACGT GTGTCTTTGG GGGGTCCGCG
  541 CGCTGCAGCG TCTCCTTCCC GTTCTCCAGG TATCTGCGGA GCCACTCCAC GCACAGGCCC
  601 TCCAGGTAGG CTCTCAGCTG CTCCGCCACA CGGGCCGCCT CCCACTTGCG CTGGGTGATC
  661 TGAGCCGCGG TGTCCGCCGC GGTCCAGGAG CTCAGGTCCT CGTTCAGGGC GATGTAATCC
  721 TTGCCGTCGT AGGCGGACTG GTCATGCCCG CGGAGGAGGC GCCCGTCGGG CCCCAGGTCG
  781 CAGCCATACA TCCTCTGGAT GATGTGAGAC CCGGCCTCGC TCTGGTTGTA GTAGCCGCGC
  841 AGGTTCCGCA GGCTCTCTCG GTAAGTCTGT GTGTTGGTCT TGAAGATCTG TGTGTTCCGG
  901 TCCCAATACT CCGGCCCCTC CTGCTCTATC CATGGCGCCC GGGGCTCCGT CCTCGGACTC
  961 GCGGCGTCGC TGTCGAACCC TCACGAAACT GGGGTGTCGT CCACGTAGCC CACTGCGATG
 1021 AAAGCGGGGC CTCCCCGCGG CCGGGCCGGG AACATGACGG GTGGTAGAAA TACCTCATGC
1081 AGTTGGGAGC GGGCTCAGGA TCTCGGATCA AGGACCACTG GATTAC
//
Primer: SV40pro_F_V2 RDB13692_A5Jgc.seq
>02876_13692_A5Jg_SV40pro_F_V2_G05_20.ab1
    1 GTAAGAACTT TTTGGAGGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGT ATATCCATTT
   61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
  121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
  181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
  241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
  301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
  361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
  421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
  481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
  541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
  601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
  661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
  721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
  781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
  841 CTCCCGATTC GCAGCGCATC GGCCTTCTAT CGGCCTTCTT GACGAGTTTC TTCTGAGCGG
  901 GACTCTGGGG GTTTCGAAAT GAACCGACCA AGCGGACGGC CCAACCCTGC CATCACGAGA
  961 TTTTCGAATT CCACCCGCCC GCCCTTCTAT GGAAAGGGTT GGGGCTTTCG GGAATCGGTT
 1021 TTTCCCGGGG ACGGCCCGGC CTGGGATGAA TCCTTCCCAG CGCGGGGGGG ATACTCATGC
1081 CTGGGGAAGG TTC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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