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Human HLA-B*15:01:01 cDNA

Human HLA-B*15:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02875
Resource name Human HLA-B*15:01:01 cDNA
Alternative name HLA-B*15011
Clone info. Human HLA-B*15:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB02875 Human HLA-B*15:01:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02875z.seq
          Remarks, protocol and/or map (gif) RDB02875.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18376_B0Hbp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB18376_B0Hba.seq check
          >02875_18376_B0Hb_2_CMV-Forward_A07_01_ABI24.ab1
              1 NNNNNNNNNN NNNTTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTCACCCG GACTCAGAAT
            181 CTCCTCAGAC GCCGAGATGC GGGTCACGGC GCCCCGAACC GTCCTCCTGC TGCTCTCGGG
            241 AGCCCTGGCC CTGACCGAGA CCTGGGCCGG CTCCCACTCC ATGAGGTATT TCTACACCGC
            301 CATGTCCCGG CCCGGCCGCG GGGAGCCCCG CTTCATCGCA GTGGGCTACG TGGACGACAC
            361 CCAGTTCGTG AGGTTCGACA GCGACGCCGC GAGTCCGAGG ATGGCGCCCC GGGCGCCATG
            421 GATAGAGCAG GAGGGGCCGG AGTATTGGGA CCGGGAGACA CAGATCTCCA AGACCAACAC
            481 ACAGACTTAC CGAGAGAGCC TGCGGAACCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG
            541 GTCTCACACC CTCCAGAGGA TGTACGGCTG CGACGTGGGG CCGGACGGGC GCCTCCTCCG
            601 CGGGCATGAC CAGTCCGCCT ACGACGGCAA GGNNTACATC GCCCTGAACG AGGACCTGAG
            661 CTCCTGGACC GCGGCGGACA CGGCGGCTCA GATCACCCAG CGCAAGTGGG AGGCGGCCCG
            721 TGNNGCGGAN CAGTGGAGAG CCTACCTGGA NGGCCTGTGC GTGGAGTGNC TCCGCAGATA
            781 CCTGGAGAAC GGGAANGANA NGCTGCAGCG CGCGGANCCC CCAAAGACAC ATGTGANCCA
            841 CCACCCCNTC TCTGANCATG ANGCCACCCT GNNGTGCTGG GCCCTGGGCT TCTANCCTGC
            901 GGANATNACA CNGNNNNNGC AGCGGGNNNG CGANGANCNA ANNNNNNANN CCGANCTNGT
            961 GNNNANCAGA NCAGCNNNNN NANNNNCNNT NNNNNNNNNG GCNNCNNNNG NNNNNNCNNN
          1021 NNNGNNNNNN NNNN
          //
          Primer: bGH_rev3
          Region: bGHpA,insert 3'
          Sequence file: RDB18376_B0Hbb.seq check
          >02875_18376_B0Hb_2_bGH_rev3_B10_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGGNN GNNGGNNNNN NACAGATGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC
            121 TCACAAGACA GCTGTCTCAG GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC
            181 TGTCGCTGGA CGCAGCCTGA GAGTAGCTCC CTCCTTTTCC ACCTGAGCTC TTCCTCCTAC
            241 ACATCACAGT AGCGACCACA GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA
            301 CAATGCCCAC GATGGGGATG GTGGACTGGG AAGATGGCTC CCATCTCAGG GTGAGGGGCT
            361 TCGGCAGCCC CTCATGCTGT ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA
            421 CCACAGCTGC CCACTTCTGG AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCGG
            481 TGTCCTGAGT TTGGTCCTCG CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA
            541 AGCCCAGGGC CCAGCACCTC AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACAT
            601 GTGTCTTTGG GGGGTCCGCG CGCTGCAGCG TCTCCTTCCC GTTCTCCAGG TATCTGCGGA
            661 GCCACTCCAC GCACAGGCCC TCCAGGTANG CTCTCCACTG CTCCGCCTCA CGGGCCGCCT
            721 CCCACTTGCG CTGGGTGATC TGANCCGCCG TGTCCGCCGC GGTCCAGGAN CTCANGTCCT
            781 CGTTCANGGC GATGTAATCC TTGCCGTCGT NNNGGNNTGG TCATGCCCGC GGANGANGCG
            841 CCCGTCCGGC CCCACGTCGC ANCCGTACNT CCNCNNGANG GNGTGANANC CNGCCNNGCN
            901 NNNGNTGNAN TANCCGCGCN NNTTCCNNNN NTCTNNCGGN NANNCTGNNN NNNNGNNNNN
          961 NNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18376_B0Hbc.seq check
          >02875_18376_B0Hb_2_SV40pro_ori_F_C07_07_ABI24.ab1
              1 NNNNNNNNNN NNNCCNCTGC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG CTTTTTTGGA
             61 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA
            121 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG
            181 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT
            241 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC
            301 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG
            361 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG
            421 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA
            481 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA
            541 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT
            601 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC
            661 AGGCTCAAGG CGCGCATGCC CGACGGCGAN GATCTCGTCG TGANCCATGG CGATGCCTGC
            721 TTGCCGAATA TCATGGTGGA AAATGGNCGC TTTTCTGGAN TCATCGACTG TGGCCGGCTG
            781 GGTGTGGCGG ANCGCTATCA NGANATAGCG TTGGCTACNC GNGANNNNGC TGAANANCTT
            841 GGCGGCGAAN GGGCTGANCG CTNNCTCGNG CTTTNCNGTA TCGCCGCTCC NNANNCNCAN
            901 CGCATCGCCN TCNATCGNNN TNNNGNNNAN TTCNTNNNNN CNGGNNNNNN GGGNNCNNAA
          961 TGANNNANNN NNCGANNNCN NNNCNNGNNN TCNCGANNNT NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl