Resource data sheet
Prev.

Human HLA-B*15:01:01 cDNA

Human HLA-B*15:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02875
Resource name Human HLA-B*15:01:01 cDNA
Alternative name HLA-B*15011
Clone info. Human HLA-B*15:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02875 Human HLA-B*15:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02875z.seq from Depositor
Electronic file Map RDB02875.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13685_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13685_A5Jga.seq
>02875_13685_A5Jg_CMV-Forward_B05_05.ab1
    1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTCACCC GGACTCAGAA
  181 TCTCCTCAGA CGCCGAGATG CGGGTCACGG CGCCCCGAAC CGTCCTCCTG CTGCTCTCGG
  241 GAGCCCTGGC CCTGACCGAG ACCTGGGCCG GCTCCCACTC CATGAGGTAT TTCTACACCG
  301 CCATGTCCCG GCCCGGCCGC GGGGAGCCCC GCTTCATCGC AGTGGGCTAC GTGGACGACA
  361 CCCAGTTCGT GAGGTTCGAC AGCGACGCCG CGAGTCCGAG GATGGCGCCC CGGGCGCCAT
  421 GGATAGAGCA GGAGGGGCCG GAGTATTGGG ACCGGGAGAC ACAGATCTCC AAGACCAACA
  481 CACAGACTTA CCGAGAGAGC CTGCGGAACC TGCGCGGCTA CTACAACCAG AGCGAGGCCG
  541 GGTCTCACAC CCTCCAGAGG ATGTACGGCT GCGACGTGGG GCCGGACGGG CGCCTCCTCC
  601 GCGGGCATGA CCAGTCCGCC TACGACGGCA AGGATTACAT CGCCCTGAAC GAGGACCTGA
  661 GCTCCTGGAC CGCGGCGGAC ACGGCGGCTC AGATCACCCA GCGCAAGTGG GAGGCGGCCC
  721 GTGAGGCGGA GCAGTGGAGA GCCTACCTGG AGGGCCTGTG CGTGGAGTGG CTCCGCAGAT
  781 ACCTGGAGAA CGGGAAGGAG ACGCTGCAGC GCGCGGACCC CCCAAAGACA CATGTGACCC
  841 ACCACCCCAT CTCTGACCAT GAGGCCACCC TGAGGTGCTG GGCCCTGGGC TTCTACCCTG
  901 CGGAGATCAC ACTGACCTGG CAGCGGGATG GCGAGGACCA AACTCAGGAC ACCGAGCTTG
  961 TGGAGACCAG ACCAGCACGA GATAGAACCC TTCCAGAAGT GGGCAGCTGT GGGTGGTGCC
 1021 CTTCTGGAGA AGAGCAGAGA TACACATGCC CATGTACAGC ATGAAGGGCT GGCCGGAGCC
 1081 CCTCACCCTG AGATGGGAGT CAATCTTCCA TTCAACATCC CCTCATCGAT GGGGGCCAAT
1141 TTGG
//
Primer: BGH_rev RDB13685_A5Jgb.seq
>02875_13685_A5Jg_BGHrev_C05_08.ab1
    1 GTTCGGGGGA CTCTAGACTC GAGCGGCCGC ATCTCAGTCC CTCACAAGAC AGCTGTCTCA
   61 GGCTTTTCAA GCTGTGAGAG ACACATCAGA GCCCTGGGCA CTGTCGCTGG ACGCAGCCTG
  121 AGAGTAGCTC CCTCCTTTTC CACCTGAGCT CTTCCTCCTA CACATCACAG TAGCGACCAC
  181 AGCTCCGATG ACCACAACTG CTAGGACAGC CAGGCCAGCA ACAATGCCCA CGATGGGGAT
  241 GGTGGACTGG GAAGATGGCT CCCATCTCAG GGTGAGGGGC TTCGGCAGCC CCTCATGCTG
  301 TACATGGCAT GTGTATCTCT GCTCTTCTCC AGAAGGCACC ACCACAGCTG CCCACTTCTG
  361 GAAGGTTCTA TCTCCTGCTG GTCTGGTCTC CACAAGCTCG GTGTCCTGAG TTTGGTCCTC
  421 GCCATCCCGC TGCCAGGTCA GTGTGATCTC CGCAGGGTAG AAGCCCAGGG CCCAGCACCT
  481 CAGGGTGGCC TCATGGTCAG AGATGGGGTG GTGGGTCACA TGTGTCTTTG GGGGGTCCGC
  541 GCGCTGCAGC GTCTCCTTCC CGTTCTCCAG GTATCTGCGG AGCCACTCCA CGCACAGGCC
  601 CTCCAGGTAG GCTCTCCACT GCTCCGCCTC ACGGGCCGCC TCCCACTTGC GCTGGGTGAT
  661 CTGAGCCGCC GTGTCCGCCG CGGTCCAGGA GCTCAGGTCC TCGTTCAGGG CGATGTAATC
  721 CTTGCCGTCG TAGGCGGACT GGTCATGCCC GCGGAGGAGG CGCCCGTCCG GCCCCACGTC
  781 GCAGCCGTAC ATCCTCTGGA GGGTGTGAGA CCCGGCCTCG CTCTGGTTGT AGTAGCCGCG
  841 CAGGTTCCGC AGGCTCTCTC GGTAAGTCTG TGTGTTGGTC TTGGAGATCT GTGTCTCCCG
  901 GTCCCAATAC TCCGGCCCCT CCTGCTCTAT CCATGGCGCC CGGGGCGCCA TCCTCGGACT
  961 CGCGGCGTCG CTGTCGAACC TCACGGAACT GGGTGTCGTC CACGTAGCCC ACTGCGATGA
 1021 AGCGGGGCTT CCCCGCGGGC CGGGCCGGGA CATGGGCGGT GTAGAAATAC CTTCATGGAA
 1081 GTGGGGAGCC GACCAGATCT CGATCAGGCC CAGGGCCTCC CGAGAAGCCA GCCAAGGGGA
1141 AGAGG
//
Primer: SV40pro_F_V2 RDB13685_A5Jgc.seq
>02875_13685_A5Jg_SV40pro_F_V2_E09_15.ab1
    1 CTGCCCTTTT TTGGAGGCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGTA TATCCATTTT
   61 CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
  121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
  181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
  241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
  301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
  361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
  421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
  481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
  541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
  601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
  661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
  721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
  781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
  841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
  901 CTGGGGGTTC GAAAATGACC CGACCAAGCG ACGCCCCAAC CTGCCATCAC GAGATTTCGA
  961 TTTCCACCGC CCGCCCTTCT ATGAAAAGGT TTGGGGCTTC GGAAATCGTT TTTCCGGGGA
 1021 ACGCTGGCTG GGATGAATCC TCCAGCGCCG GGGATCTCAT GCTGGGAGTT CTTCGCCCAC
 1081 CCCACCTGTT TATTTGCAGC CTTATAATGG GTAACAATTA AGCCAAATAG CCATTCCACA
1141 CAAAGTTTTT CTAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl