Resource data sheet
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Human HLA-B*07:02:01 cDNA

Human HLA-B*07:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02874
Resource name Human HLA-B*07:02:01 cDNA
Alternative name HLA-B*07021
Clone info. Human HLA-B*07:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-B (NCBI Gene 3106) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02874 Human HLA-B*07:02:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02874z.seq from Depositor
Electronic file Map RDB02874.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13690_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13690_A5Jga.seq
>02874_13690_A5Jg_CMV-Forward_G04_19.ab1
    1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTCACCC GGACTCAGAA
  181 TCTCCTCAGA CGCCGAGATG CTGGTCATGG CGCCCCGAAC CGTCCTCCTG CTGCTCTCGG
  241 CGGCCCTGGC CCTGACCGAG ACCTGGGCCG GCTCCCACTC CATGAGGTAT TTCTACACCT
  301 CCGTGTCCCG GCCCGGCCGC GGGGAGCCCC GCTTCATCTC AGTGGGCTAC GTGGACGACA
  361 CCCAGTTCGT GAGGTTCGAC AGCGACGCCG CGAGTCCGAG AGAGGAGCCG CGGGCGCCGT
  421 GGATAGAGCA GGAGGGGCCG GAGTATTGGG ACCGGAACAC ACAGATCTAC AAGGCCCAGG
  481 CACAGACTGA CCGAGAGAGC CTGCGGAACC TGCGCGGCTA CTACAACCAG AGCGAGGCCG
  541 GGTCTCACAC CCTCCAGAGC ATGTACGGCT GCGACGTGGG GCCGGACGGG CGCCTCCTCC
  601 GCGGGCATGA CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAC GAGGACCTGC
  661 GCTCCTGGAC CGCCGCGGAC ACGGCGGCTC AGATCACCCA GCGCAAGTGG GAGGCGGCCC
  721 GTGAGGCGGA GCAGCGGAGA GCCTACCTGG AGGGCGAGTG CGTGGAGTGG CTCCGCAGAT
  781 ACCTGGAGAA CGGGAAGGAC AAGCTGGAGC GCGCTGACCC CCCAAAGACA CACGTGACCC
  841 ACCACCCCAT CTCTGACCAT GAGGCCACCC TGAGGTGCTG GGCCCTGGGT TTCTACCCTG
  901 CGGAGATCAC ACTGACCTGG CAGCGGGATG GCGAGGACCA AACTCAGGAC ACTGAGCTTT
  961 GTGGAGACCA GACCAGCAGA GATAGAACCT TTCCAGAAGT GGGCAGCTGT GGTGGTGCCT
 1021 TCTGGAGAAG GAGCAGAGAT ACACATGCAT GTACAGCATG AGGGGCTGCG GAGGCCCCTC
 1081 ACCCTGAGAT GGGAGCCGTC TCCCAGTCAC GATCCCCAAT CGTTGGCATT GTTTGGCTGG
1141 GACCTTGACG
//
Primer: BGH_rev RDB13690_A5Jgb.seq
>02874_13690_A5Jg_BGHrev_H04_22.ab1
    1 GATTCGGGTC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC TCACAAGACA GCTGTCTCAG
   61 GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC TGTCGCTGCA CGCAGCCTGA
  121 GAGTAGCTCC CTCCTTTTCC ACCTGAACTC TTCCTCCTAC ACATCACAGC AGCGACCACA
  181 GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA CAATGCCCAC GATGGGGACG
  241 GTGGACTGGG AAGACGGCTC CCATCTCAGG GTGAGGGGCT TCGGCAGCCC CTCATGCTGT
  301 ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA CCACAGCTGC CCACTTCTGG
  361 AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG TGTCCTGAGT TTGGTCCTCG
  421 CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA AACCCAGGGC CCAGCACCTC
  481 AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACGT GTGTCTTTGG GGGGTCAGCG
  541 CGCTCCAGCT TGTCCTTCCC GTTCTCCAGG TATCTGCGGA GCCACTCCAC GCACTCGCCC
  601 TCCAGGTAGG CTCTCCGCTG CTCCGCCTCA CGGGCCGCCT CCCACTTGCG CTGGGTGATC
  661 TGAGCCGCCG TGTCCGCGGC GGTCCAGGAG CGCAGGTCCT CGTTCAGGGC GATGTAATCC
  721 TTGCCGTCGT AGCGTACTGG TCATGCCCGC GGAGGAGGCG CCCGTCCGGC CCCACGTCGC
  781 AGCCGTACAT GCTCTGGAGG GTGTGAGACC CGGGCCTCGC TCTGGGTTGT AGTAGCCGCG
  841 CAGGTTCCGC AGGCTCTCTC GGGTCAGTCT GTGCCTGGGC CTTGTAGATC TGTGTGTTCC
  901 CGGGTCCCAA TACTCCGGGC CCCTCTGCTC TATCCACGGG CGCCGCGGCT CTCTCTCGGA
  961 CTCGCGGGCG TCGCTGTCGA TCCTCACGGA AACTGGGTGT CCGTTCAACG TAGCCCACTG
 1021 GAGATTGGAA GCCGGGGCTT CCCCCCGCGA CCAGGACTGG CAACTCGGAA GTGTTAGAAA
1081 TAACCCTCAT TG
//
Primer: SV40pro_F_V2 RDB13690_A5Jgc.seq
>02874_13690_A5Jg_SV40pro_F_V2_A05_02.ab1
    1 CAGCGCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
   61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
  841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
  901 GGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA
  961 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC CGGCTGGATG
 1021 ATCCTCCAGC GCGGGGGATC TCATGCTGGA GTTCTTCGCC CCACCCCACT GTTATTGCAG
 1081 CTTATAATGG TACCACTAAG CCAATTAGCA TCACAAATTT CCACAAAATA AAGCCAACTT
1141 CATCTC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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