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Human HLA-B*07:02:01 cDNA

Human HLA-B*07:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02874
Resource name Human HLA-B*07:02:01 cDNA
Alternative name HLA-B*07021
Clone info. Human HLA-B*07:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human MHC class I B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB02874 Human HLA-B*07:02:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02874z.seq
          Remarks, protocol and/or map (gif) RDB02874.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13690_A5Jgp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert 5'
          Sequence file: RDB13690_A5Jga.seq check
          >02874_13690_A5Jg_CMV-Forward_G04_19.ab1
              1 NNNNNNNNNN NNNNNTNNNG CNNNGGGCGG TANGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTCACCC GGACTCAGAA
            181 TCTCCTCAGA CGCCGAGATG CTGGTCATGG CGCCCCGAAC CGTCCTCCTG CTGCTCTCGG
            241 CGGCCCTGGC CCTGACCGAG ACCTGGGCCG GCTCCCACTC CATGAGGTAT TTCTACACCT
            301 CCGTGTCCCG GCCCGGCCGC GGGGAGCCCC GCTTCATCTC AGTGGGCTAC GTGGACGACA
            361 CCCAGTTCGT GAGGTTCGAC AGCGACGCCG CGAGTCCGAG AGAGGAGCCG CGGGCGCCGT
            421 GGATAGAGCA GGAGGGGCCG GAGTATTGGG ACCGGAACAC ACAGATCTAC AAGGCCCAGG
            481 CACAGACTGA CCGAGAGAGC CTGCGGAACC TGCGCGGCTA CTACAACCAG AGCGAGGCCG
            541 GGTCTCACAC CCTCCAGAGC ATGTACGGCT GCGACGTGGG GCCGGACGGG CGCCTCCTCC
            601 GCGGGCATGA CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAC GAGGACCTGC
            661 GCTCCTGGAC CGCCGCGGAC ACGGCGGCTC AGATCACCCA GCGCAAGTGG GANGCGGCCC
            721 GTGAGGCGGA NCAGCGGANA GCCTACCTGG AGGGCGAGTG CGTGGAGTGG CTCCGCAGAT
            781 ACCTGGAGAA CGGGAAGGAN AAGCTGGANC GCGCTGANCC CCCNAAGANA CACGTGANCC
            841 ACCACCCCNT CTCTGANNNT GANGNCACCC NNNNGTGCTG GGCCCNNGGT TTCTACCNTG
            901 CGGANATNNC NNNNNNCNNN NNNCGGNNTG NNGANGANNN AANTNNNNAN NNTNNNNNTN
          961 NNNNANANNA GANNANCNNN NANNNNNNNN TNNNNNNNNN N
          //
          Primer: BGH_rev2
          Region: bGH pA, insert 3'
          Sequence file: RDB13690_A5Jgd.seq check
          >02874_13690_B0GgF_BGH_rev2_C08_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGGGG GNNGGNNNNN NACAGATGGC TGGCAACTAG AANGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCA TCTCAGTCCC
            121 TCACAAGACA GCTGTCTCAG GCTTTTCAAG CTGTGAGAGA CACATCAGAG CCCTGGGCAC
            181 TGTCGCTGCA CGCAGCCTGA GAGTAGCTCC CTCCTTTTCC ACCTGAACTC TTCCTCCTAC
            241 ACATCACAGC AGCGACCACA GCTCCGATGA CCACAACTGC TAGGACAGCC AGGCCAGCAA
            301 CAATGCCCAC GATGGGGACG GTGGACTGGG AAGACGGCTC CCATCTCAGG GTGAGGGGCT
            361 TCGGCAGCCC CTCATGCTGT ACATGGCATG TGTATCTCTG CTCTTCTCCA GAAGGCACCA
            421 CCACAGCTGC CCACTTCTGG AAGGTTCTAT CTCCTGCTGG TCTGGTCTCC ACAAGCTCAG
            481 TGTCCTGAGT TTGGTCCTCG CCATCCCGCT GCCAGGTCAG TGTGATCTCC GCAGGGTAGA
            541 AACCCAGGGC CCAGCACCTC AGGGTGGCCT CATGGTCAGA GATGGGGTGG TGGGTCACGT
            601 GTGTCTTTGG GGGGTCAGCG CGCTCCAGCT TGTCCTTCCC GTTCTCCAGG TATCTGCGGA
            661 NCCACTCCAC GCACTCGCCC TCCAGGTANG CTCTCCGCTG CTCCGCCTCA CGGGCCGCCT
            721 CCCACTTGCG CTGGGTGNTC TGANCCGCCG TGTCCGCGGC GGTCCAGGAN CGCANGTCCT
            781 CGTTCANGGC GATGTNATCC TTGCCGTCGN NNNGTACNNG NCATGCCCGC GGNNNNNGCG
            841 CCCGNCCNGC CCCACNTCGC ANCNNNACAT NCTNNNGNNG GNGNNANANC NNGCCNNNNN
          901 NNNNN
          //
          Primer: SV40pro_ori_f
          Region: SV40 pro_ori, NeoR
          Sequence file: RDB13690_A5Jge.seq check
          >02874_13690_B0GgF_SV40pro_ori_F_D08_11_ABI24.ab1
              1 NNNNNNNNNN NNGNCGCCNC TGCCTCTGNN CTATTCNNGA AGTAGTGAGG ANGCTTTTTT
             61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
            121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT
            181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC
            241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
            301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC
            361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
            421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG
            481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
            541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
            601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
            661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GANGNTCTCG TCGTGACCCA TGGCGATGCC
            721 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CNGNGGCCGG
            781 CNGGGTGTGG CGGNNCGCTA TCANGNNATA NCGTTGGCNA NCCNNNANAT NGCTGAAGAN
            841 CTNNGCGGCN AANGGNNNNA NNGCNNCNNC NNNNNTTACN GNATCNNNNN NNCNNNNTNN
          901 NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl