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Human HLA-A*33:03 cDNA

Human HLA-A*33:03 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02873
Resource name Human HLA-A*33:03 cDNA
Alternative name HLA-A*3303
Clone info. Human HLA-A*33:03 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB02873 Human HLA-A*33:03 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02873z.seq
          Remarks, protocol and/or map (gif) RDB02873.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14669_A7Dcp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: insert 5'
          Sequence file: RDB14669_A7Dca.seq check
          >02873_14669_A7Dc_CMV-Forward_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNT GGGCGGTAGG CGTGNNACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTNNNNNN GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
            121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGATTCTC
            181 CCCAGACGCC GAGGATGGCC GTCATGGCGC CCCGAACCCT CCTCCTGCTA CTCTTGGGGG
            241 CCCTGGCCCT GACCCAGACC TGGGCGGGCT CCCACTCCAT GAGGTATTTC ACCACATCCG
            301 TGTCCCGGCC CGGCCGCGGG GAGCCCCGCT TCATCGCCGT GGGCTACGTG GACGACACGC
            361 AGTTCGTGCG GTTCGACAGC GACGCCGCGA GCCAGAGGAT GGAGCCGCGG GCGCCGTGGA
            421 TAGAGCAGGA GGGGCCGGAG TATTGGGACC GGAACACACG GAATGTGAAG GCCCACTCAC
            481 AGATTGACCG AGTGGACCTG GGGACCCTGC GCGGCTACTA CAACCAGAGC GAGGCCGGTT
            541 CTCACACCAT CCAGATGATG TATGGCTGCG ACGTGGGGTC GGACGGGCGC TTCCTCCGCG
            601 GGTACCAGCA GGACGCCTAC GACGGCAAGG ATTACATCGC CTTGAACGAG GACCTGCGCT
            661 CTTGGACCGC GGCGGACATG GCGGCTCAGA TCACCCAGCG CAAGTGGGAN GCGGCCCGTG
            721 TGGCGGANCA GTTGAGAGCC TACCTGGAGG GCACGTGCGT GGAGTGGCTC CGCAGATACC
            781 TGNAGAACGG GAAGGANACG CTGCAGCGCA CGGACCCCCC CCAAGACGCA TATGACTCAC
            841 CACGCTGTCT CTGANCATGA GGNNNCCCNG ANGTGCTGGN NCCNGANCTT CTACCCTGCN
            901 GNNATCACNN NGNCCTNNNN GCGGGNTGGG GGANGANCAG ANCCNNGNAN NNNGNNCTCN
          961 NNNNNNNNN
          //
          Primer: BGH_rev2
          Region: insert 3'
          Sequence file: RDB14669_A7Dcb.seq check
          >02873_14669_A7Dc_BGH_rev2_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN GGNNNNNNAC AGATGGCTGG CAACTAGAAG
             61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCACAA
            121 GGCAGCTGTC TCACACTTTA CAAGCTGTGA GAGACATATC AGAGCCCTGG GCACTGTCAC
            181 TGCTTGCAGC CTGAGAGTAG CTCCCTCCTT TTCTATCTGA GCTCTTCCTC CTCCACCTCA
            241 CAGCAGCGAC CACAGCTCCA GCGAACACAG CTCCAAAGAG AACTAGGCCA GCAATGATGC
            301 CCACGATGGG GATGGTGGGC TGGGAAGACG GCTCCCATCT CAGGGTGAGG GGCTTGGGGA
            361 GACCCTCATG CTGCACATGG CAGGTGTATC TCTGCTCCTG TCCAGAAGGC ACCACCACAG
            421 ACGCCCACTT CTGGAAGGTT CCATCCCCTG CAGGCCTGGT CTCCACGAGC TCCGTGTCCT
            481 GGGTCTGGTC CTCCCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG TAGAAGCTCA
            541 GGGCCCAGCA CCTCAGGGTG GCCTCATGGT CAGAGACAGC GTGGTGAGTC ATATGCGTCT
            601 TGGGGGGGTC CGTGCGCTGC ANCGTCTCCT TCCCGTTCTC CANGTATCTG CGGANCCACT
            661 CCACGCACGT GCCCTCCAGG TAGGCTCTCA ACTGCTCCNC CACACGGGCC GCCTCCCACT
            721 TGCGCTGGGT GATCTGANCC GCCATGTCCG CCGCGGTCCA ANANNGCANG NCCTCGTTCN
            781 ANGCGATGTA ATCCTTGCCG TCGTANGCGT CCTGCTGGTA CCCGCNGANG ANNCGCCCGT
            841 CCGNNCCCAC NTCNNNNNCA TACATCATCT GGANGGNGTG ANANNNNGNC TCNCNNTGGT
          901 TGTANTANNN NNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori, NeoR
          Sequence file: RDB14669_A7Dcc.seq check
          >02873_14669_B0G5F_SV40pro_ori_F_A09_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNCNCTGCC TCTGNNCTAT TCNNGAAGTA GTGAGGAGGC
             61 TTTTTTGGAG GCCTAGGCTT TNTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG
            121 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC
            181 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT
            241 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT
            301 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG
            361 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG
            421 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC
            481 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC
            541 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA
            601 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA
            661 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAN GNNCTCGTCG TGACCCATGG
            721 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACNG
            781 TGGCCGGCTN GNNGTGGCGG ANNGCTATCA NGNCATANCG TTGGCTACCC GNNATATNGC
            841 TGAAGANCTN NGCGGCGAAN GGGCTGANNG CTNCCNCNNN CTTNACNGNA NCGCCGCNCC
          901 NNNNTCNNAN NNCATCGNCN NTCNATCNNN NTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl