Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-A*33:03 cDNA

Human HLA-A*33:03 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02873
Resource name Human HLA-A*33:03 cDNA
Alternative name HLA-A*3303
Clone info. Human HLA-A*33:03 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02873 Human HLA-A*33:03 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02873z.seq
Electronic file Map RDB02873.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14669_A7Dcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: insert 5'
Sequence file: RDB14669_A7Dca.seq check
>02873_14669_A7Dc_CMV-Forward_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNT GGGCGGTAGG CGTGNNACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTNNNNNN GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGATTCTC
  181 CCCAGACGCC GAGGATGGCC GTCATGGCGC CCCGAACCCT CCTCCTGCTA CTCTTGGGGG
  241 CCCTGGCCCT GACCCAGACC TGGGCGGGCT CCCACTCCAT GAGGTATTTC ACCACATCCG
  301 TGTCCCGGCC CGGCCGCGGG GAGCCCCGCT TCATCGCCGT GGGCTACGTG GACGACACGC
  361 AGTTCGTGCG GTTCGACAGC GACGCCGCGA GCCAGAGGAT GGAGCCGCGG GCGCCGTGGA
  421 TAGAGCAGGA GGGGCCGGAG TATTGGGACC GGAACACACG GAATGTGAAG GCCCACTCAC
  481 AGATTGACCG AGTGGACCTG GGGACCCTGC GCGGCTACTA CAACCAGAGC GAGGCCGGTT
  541 CTCACACCAT CCAGATGATG TATGGCTGCG ACGTGGGGTC GGACGGGCGC TTCCTCCGCG
  601 GGTACCAGCA GGACGCCTAC GACGGCAAGG ATTACATCGC CTTGAACGAG GACCTGCGCT
  661 CTTGGACCGC GGCGGACATG GCGGCTCAGA TCACCCAGCG CAAGTGGGAN GCGGCCCGTG
  721 TGGCGGANCA GTTGAGAGCC TACCTGGAGG GCACGTGCGT GGAGTGGCTC CGCAGATACC
  781 TGNAGAACGG GAAGGANACG CTGCAGCGCA CGGACCCCCC CCAAGACGCA TATGACTCAC
  841 CACGCTGTCT CTGANCATGA GGNNNCCCNG ANGTGCTGGN NCCNGANCTT CTACCCTGCN
  901 GNNATCACNN NGNCCTNNNN GCGGGNTGGG GGANGANCAG ANCCNNGNAN NNNGNNCTCN
961 NNNNNNNNN
//
Primer: BGH_rev2
Region: insert 3'
Sequence file: RDB14669_A7Dcb.seq check
>02873_14669_A7Dc_BGH_rev2_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN GGNNNNNNAC AGATGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCACAA
  121 GGCAGCTGTC TCACACTTTA CAAGCTGTGA GAGACATATC AGAGCCCTGG GCACTGTCAC
  181 TGCTTGCAGC CTGAGAGTAG CTCCCTCCTT TTCTATCTGA GCTCTTCCTC CTCCACCTCA
  241 CAGCAGCGAC CACAGCTCCA GCGAACACAG CTCCAAAGAG AACTAGGCCA GCAATGATGC
  301 CCACGATGGG GATGGTGGGC TGGGAAGACG GCTCCCATCT CAGGGTGAGG GGCTTGGGGA
  361 GACCCTCATG CTGCACATGG CAGGTGTATC TCTGCTCCTG TCCAGAAGGC ACCACCACAG
  421 ACGCCCACTT CTGGAAGGTT CCATCCCCTG CAGGCCTGGT CTCCACGAGC TCCGTGTCCT
  481 GGGTCTGGTC CTCCCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG TAGAAGCTCA
  541 GGGCCCAGCA CCTCAGGGTG GCCTCATGGT CAGAGACAGC GTGGTGAGTC ATATGCGTCT
  601 TGGGGGGGTC CGTGCGCTGC ANCGTCTCCT TCCCGTTCTC CANGTATCTG CGGANCCACT
  661 CCACGCACGT GCCCTCCAGG TAGGCTCTCA ACTGCTCCNC CACACGGGCC GCCTCCCACT
  721 TGCGCTGGGT GATCTGANCC GCCATGTCCG CCGCGGTCCA ANANNGCANG NCCTCGTTCN
  781 ANGCGATGTA ATCCTTGCCG TCGTANGCGT CCTGCTGGTA CCCGCNGANG ANNCGCCCGT
  841 CCGNNCCCAC NTCNNNNNCA TACATCATCT GGANGGNGTG ANANNNNGNC TCNCNNTGGT
901 TGTANTANNN NNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40pro_ori, NeoR
Sequence file: RDB14669_A7Dcc.seq check
>02873_14669_B0G5F_SV40pro_ori_F_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCNCTGCC TCTGNNCTAT TCNNGAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GCCTAGGCTT TNTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG
  121 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC
  181 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT
  241 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT
  301 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG
  361 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG
  421 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC
  481 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC
  541 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA
  601 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA
  661 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAN GNNCTCGTCG TGACCCATGG
  721 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACNG
  781 TGGCCGGCTN GNNGTGGCGG ANNGCTATCA NGNCATANCG TTGGCTACCC GNNATATNGC
  841 TGAAGANCTN NGCGGCGAAN GGGCTGANNG CTNCCNCNNN CTTNACNGNA NCGCCGCNCC
901 NNNNTCNNAN NNCATCGNCN NTCNATCNNN NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.07.09

GNP_filter3_RDBDEP_html_200316.pl