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Human HLA-A*33:03 cDNA

Human HLA-A*33:03 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02873
Resource name Human HLA-A*33:03 cDNA
Alternative name HLA-A*3303
Clone info. Human HLA-A*33:03 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02873 Human HLA-A*33:03 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02873z.seq from Depositor
Electronic file Map RDB02873.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02873_A2Kip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_long RDB02873_A2Kia.seq
>02873_02873_A2Ki_T7Long_C03_09.ab1
    1 CAAAGCTGGC TAGCGTTTAA CTTAAGCTTG ATTCTCCCCA GACGCCGAGG ATGGCCGTCA
   61 TGGCGCCCCG AACCCTCCTC CTGCTACTCT TGGGGGCCCT GGCCCTGACC CAGACCTGGG
  121 CGGGCTCCCA CTCCATGAGG TATTTCACCA CATCCGTGTC CCGGCCCGGC CGCGGGGAGC
  181 CCCGCTTCAT CGCCGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC GACAGCGACG
  241 CCGCGAGCCA GAGGATGGAG CCGCGGGCGC CGTGGATAGA GCAGGAGGGG CCGGAGTATT
  301 GGGACCGGAA CACACGGAAT GTGAAGGCCC ACTCACAGAT TGACCGAGTG GACCTGGGGA
  361 CCCTGCGCGG CTACTACAAC CAGAGCGAGG CCGGTTCTCA CACCATCCAG ATGATGTATG
  421 GCTGCGACGT GGGGTCGGAC GGGCGCTTCC TCCGCGGGTA CCAGCAGGAC GCCTACGACG
  481 GCAAGGATTA CATCGCCTTG AACGAGGACC TGCGCTCTTG GACCGCGGCG GACATGGCGG
  541 CTCAGATCAC CCAGCGCAAG TGGGAGGCGG CCCGTGTGGC GGAGCAGTTG AGAGCCTACC
  601 TGGAGGGCAC GTGCGTGGAG TGGCTCCGCA GATACCTGGA GAACGGGAAG GAGACGCTGC
  661 AGCGCACGGA CCCCCCCAAG ACGCATATGA CTCACCACGC TGTCTCTGAC CATGAGGCCA
  721 CCCTGAGGTG CTGGGCCCTG AGCTTCTACC CTGCGGAGAT CACACTGACC TGGCAGCGGG
  781 ATGGGGAGGA CCAGACCCAG GACACGGAGC TCGTGGAGAC CAGGCCTGCA GGGGATGGAA
  841 CCTTCCAGAA GTGGGCGTCT GTGGTGGTGC CTTCTGGACA GGAGCAGAGA TACACCTGCC
  901 ATGTGCAGCA TGAGGGTCTC CCCAAGCCCC TCACCCTGAG ATGGGAGCCG TCTTCCCAGC
  961 CCACCATCCC CATCGTGGGC ATCATTGCTG GGCCTAGTTC TCTTTGGAGC TGTGTTCGCT
 1021 GGAGCTGTGT CGCTGCCTGT GAGTGCAGAG AAGAGCTCAG ATAAGAAAAA GAGGAAGCTA
 1081 CTCCTCAAGC TGGCAAGCAG TGACAGTGCT AAGGCTCTGA ATATGTTCTC TCACAGCCTT
1141 GTTAAGGTGT GTGAGAAACA GCCTGC
//
Primer: BGH_rev RDB02873_A2Kib.seq
>02873_02873_A2Ki_BGH_rev_D03_12.ab1
    1 GTTCGGGTCT CTAGACTCGA GCGGCCGCAC AAGGCAGCTG TCTCACACTT TACAAGCTGT
   61 GAGAGACATA TCAGAGCCCT GGGCACTGTC ACTGCTTGCA GCCTGAGAGT AGCTCCCTCC
  121 TTTTCTATCT GAGCTCTTCC TCCTCCACCT CACAGCAGCG ACCACAGCTC CAGCGAACAC
  181 AGCTCCAAAG AGAACTAGGC CAGCAATGAT GCCCACGATG GGGATGGTGG GCTGGGAAGA
  241 CGGCTCCCAT CTCAGGGTGA GGGGCTTGGG GAGACCCTCA TGCTGCACAT GGCAGGTGTA
  301 TCTCTGCTCC TGTCCAGAAG GCACCACCAC AGACGCCCAC TTCTGGAAGG TTCCATCCCC
  361 TGCAGGCCTG GTCTCCACGA GCTCCGTGTC CTGGGTCTGG TCCTCCCCAT CCCGCTGCCA
  421 GGTCAGTGTG ATCTCCGCAG GGTAGAAGCT CAGGGCCCAG CACCTCAGGG TGGCCTCATG
  481 GTCAGAGACA GCGTGGTGAG TCATATGCGT CTTGGGGGGG TCCGTGCGCT GCAGCGTCTC
  541 CTTCCCGTTC TCCAGGTATC TGCGGAGCCA CTCCACGCAC GTGCCCTCCA GGTAGGCTCT
  601 CAACTGCTCC GCCACACGGG CCGCCTCCCA CTTGCGCTGG GTGATCTGAG CCGCCATGTC
  661 CGCCGCGGTC CAAGAGCGCA GGTCCTCGTT CAAGGCGATG TAATCCTTGC CGTCGTAGGC
  721 GTCCTGCTGG TACCCGCGGA GGAAGCGCCC GTCCGACCCC ACGTCGCAGC CATACATCAT
  781 CTGGATGGTG TGAGAACCGG CCTCGCTCTG GTTGTAGTAG CCGCGCAGGG TCCCCAGGTC
  841 CACTCGGTCA ATCTGTGAGT GGGCCTTCAC ATTCCGTGTG TTCCGGTCCC AATACTCCGG
  901 CCCCTCCTGC TCTATCCACG GCGCCCGCGG CTCCATCCTC TGGCTCGCGG CGTCGCTGTC
  961 GAACCGCACG AACTGCGTGT CGTCCACGTA GCCCACGGCG ATGAAGCGGG GCTCCCCGCG
 1021 GCCGGGCCGG GACACGGATG TGGTGAAATA CCCTCATGAG TGGAGCCGCC CAGGTCTGGG
 1081 TCATGACAGG CCCCCAGAGT AGCAGAGGAA GCATCCGGGC GCATGACCGT CAATCATCGG
1141 CGTCTTGGGG GGAGAAAATC CAAGGCCTTA AGGAT
//
Primer: SV40pro_F_V2 RDB02873_A2Kic.seq
>02873_02873_A2Ki_SV40pro_F_V2_A11_02.ab1
    1 ATGAGGGGTT TTTTGGGGGG CCTAGGCTTT TGCAAAAGCT CCCGGGAGCT TGTATATCCA
   61 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
  121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
  181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
  241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
  301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
  361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
  421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
  481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA ACACGTACTC
  541 GGATGGAAGC CGGTCTTGTC AATCAGGATG ATCTGGACGA AAGAGCATCA AGGGCTCGCG
601 CCAGCCGAAC TGTTCGCCAG GCTCAAAGCG CCCATGCCCT A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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