Human HLA-A*33:03 cDNA from human EBV-transformed B cell line cDNA
| Catalog number | RDB02873 |
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| Resource name | Human HLA-A*33:03 cDNA |
| Alternative name | HLA-A*3303 |
| Clone info. | Human HLA-A*33:03 cDNA from human EBV-transformed B cell line cDNA. |
| Vector backbone | pcDNA3.1(+) (Plasmid) |
| Size of vector backbone | 5.4 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human major histocompatibility complex, class I, A cDNA |
| Depositor | Akatsuka, Yoshiki | |
| Other clones in our bank | human HLA-A (NCBI Gene 3105) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).). |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB02873 | Human HLA-A*33:03 cDNA | DNA solution |
Electronic file
| Electronic file | Sequence RDB02873z.seq from Depositor |
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| Electronic file | Map RDB02873.gif from Depositor
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Featured content
| Featured content | HLA Clones (en) (English text) |
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| Featured content | HLA Clones (ja) (Japanese text) |
References
| original | Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB02873_A2Kip1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_long | RDB02873_A2Kia.seq |
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>02873_02873_A2Ki_T7Long_C03_09.ab1 1 CAAAGCTGGC TAGCGTTTAA CTTAAGCTTG ATTCTCCCCA GACGCCGAGG ATGGCCGTCA 61 TGGCGCCCCG AACCCTCCTC CTGCTACTCT TGGGGGCCCT GGCCCTGACC CAGACCTGGG 121 CGGGCTCCCA CTCCATGAGG TATTTCACCA CATCCGTGTC CCGGCCCGGC CGCGGGGAGC 181 CCCGCTTCAT CGCCGTGGGC TACGTGGACG ACACGCAGTT CGTGCGGTTC GACAGCGACG 241 CCGCGAGCCA GAGGATGGAG CCGCGGGCGC CGTGGATAGA GCAGGAGGGG CCGGAGTATT 301 GGGACCGGAA CACACGGAAT GTGAAGGCCC ACTCACAGAT TGACCGAGTG GACCTGGGGA 361 CCCTGCGCGG CTACTACAAC CAGAGCGAGG CCGGTTCTCA CACCATCCAG ATGATGTATG 421 GCTGCGACGT GGGGTCGGAC GGGCGCTTCC TCCGCGGGTA CCAGCAGGAC GCCTACGACG 481 GCAAGGATTA CATCGCCTTG AACGAGGACC TGCGCTCTTG GACCGCGGCG GACATGGCGG 541 CTCAGATCAC CCAGCGCAAG TGGGAGGCGG CCCGTGTGGC GGAGCAGTTG AGAGCCTACC 601 TGGAGGGCAC GTGCGTGGAG TGGCTCCGCA GATACCTGGA GAACGGGAAG GAGACGCTGC 661 AGCGCACGGA CCCCCCCAAG ACGCATATGA CTCACCACGC TGTCTCTGAC CATGAGGCCA 721 CCCTGAGGTG CTGGGCCCTG AGCTTCTACC CTGCGGAGAT CACACTGACC TGGCAGCGGG 781 ATGGGGAGGA CCAGACCCAG GACACGGAGC TCGTGGAGAC CAGGCCTGCA GGGGATGGAA 841 CCTTCCAGAA GTGGGCGTCT GTGGTGGTGC CTTCTGGACA GGAGCAGAGA TACACCTGCC 901 ATGTGCAGCA TGAGGGTCTC CCCAAGCCCC TCACCCTGAG ATGGGAGCCG TCTTCCCAGC 961 CCACCATCCC CATCGTGGGC ATCATTGCTG GGCCTAGTTC TCTTTGGAGC TGTGTTCGCT 1021 GGAGCTGTGT CGCTGCCTGT GAGTGCAGAG AAGAGCTCAG ATAAGAAAAA GAGGAAGCTA 1081 CTCCTCAAGC TGGCAAGCAG TGACAGTGCT AAGGCTCTGA ATATGTTCTC TCACAGCCTT 1141 GTTAAGGTGT GTGAGAAACA GCCTGC // |
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| Primer: BGH_rev | RDB02873_A2Kib.seq |
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>02873_02873_A2Ki_BGH_rev_D03_12.ab1 1 GTTCGGGTCT CTAGACTCGA GCGGCCGCAC AAGGCAGCTG TCTCACACTT TACAAGCTGT 61 GAGAGACATA TCAGAGCCCT GGGCACTGTC ACTGCTTGCA GCCTGAGAGT AGCTCCCTCC 121 TTTTCTATCT GAGCTCTTCC TCCTCCACCT CACAGCAGCG ACCACAGCTC CAGCGAACAC 181 AGCTCCAAAG AGAACTAGGC CAGCAATGAT GCCCACGATG GGGATGGTGG GCTGGGAAGA 241 CGGCTCCCAT CTCAGGGTGA GGGGCTTGGG GAGACCCTCA TGCTGCACAT GGCAGGTGTA 301 TCTCTGCTCC TGTCCAGAAG GCACCACCAC AGACGCCCAC TTCTGGAAGG TTCCATCCCC 361 TGCAGGCCTG GTCTCCACGA GCTCCGTGTC CTGGGTCTGG TCCTCCCCAT CCCGCTGCCA 421 GGTCAGTGTG ATCTCCGCAG GGTAGAAGCT CAGGGCCCAG CACCTCAGGG TGGCCTCATG 481 GTCAGAGACA GCGTGGTGAG TCATATGCGT CTTGGGGGGG TCCGTGCGCT GCAGCGTCTC 541 CTTCCCGTTC TCCAGGTATC TGCGGAGCCA CTCCACGCAC GTGCCCTCCA GGTAGGCTCT 601 CAACTGCTCC GCCACACGGG CCGCCTCCCA CTTGCGCTGG GTGATCTGAG CCGCCATGTC 661 CGCCGCGGTC CAAGAGCGCA GGTCCTCGTT CAAGGCGATG TAATCCTTGC CGTCGTAGGC 721 GTCCTGCTGG TACCCGCGGA GGAAGCGCCC GTCCGACCCC ACGTCGCAGC CATACATCAT 781 CTGGATGGTG TGAGAACCGG CCTCGCTCTG GTTGTAGTAG CCGCGCAGGG TCCCCAGGTC 841 CACTCGGTCA ATCTGTGAGT GGGCCTTCAC ATTCCGTGTG TTCCGGTCCC AATACTCCGG 901 CCCCTCCTGC TCTATCCACG GCGCCCGCGG CTCCATCCTC TGGCTCGCGG CGTCGCTGTC 961 GAACCGCACG AACTGCGTGT CGTCCACGTA GCCCACGGCG ATGAAGCGGG GCTCCCCGCG 1021 GCCGGGCCGG GACACGGATG TGGTGAAATA CCCTCATGAG TGGAGCCGCC CAGGTCTGGG 1081 TCATGACAGG CCCCCAGAGT AGCAGAGGAA GCATCCGGGC GCATGACCGT CAATCATCGG 1141 CGTCTTGGGG GGAGAAAATC CAAGGCCTTA AGGAT // |
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| Primer: SV40pro_F_V2 | RDB02873_A2Kic.seq |
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>02873_02873_A2Ki_SV40pro_F_V2_A11_02.ab1 1 ATGAGGGGTT TTTTGGGGGG CCTAGGCTTT TGCAAAAGCT CCCGGGAGCT TGTATATCCA 61 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT 121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA ACACGTACTC 541 GGATGGAAGC CGGTCTTGTC AATCAGGATG ATCTGGACGA AAGAGCATCA AGGGCTCGCG 601 CCAGCCGAAC TGTTCGCCAG GCTCAAAGCG CCCATGCCCT A // |
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Sequencing and PCR primers for primer information.
References
| original | Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. |
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2018.12.11
GNP_filter3_RDBDEP_html_181012.pl