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Human HLA-A*31:01:02 cDNA

Human HLA-A*31:01:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02872
Resource name Human HLA-A*31:01:02 cDNA
Alternative name HLA-A*31012
Clone info. Human HLA-A*31:01:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB02872 Human HLA-A*31:01:02 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02872z.seq
          Remarks, protocol and/or map (gif) RDB02872.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13689_A5Jgp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB13689_A5Jga.seq check
          >02872_13689_A5Jg_CMV-Forward_D04_10.ab1
              1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGATTC TCCCCAGACG
            181 CCGAGGATGG CCGTCATGGC GCCCCGAACC CTCCTCCTGC TACTCTTGGG GGCCCTGGCC
            241 CTGACCCAGA CCTGGGCGGG CTCCCACTCC ATGAGGTATT TCACCACATC CGTGTCCCGG
            301 CCCGGCCGCG GGGAGCCCCG CTTCATCGCC GTGGGCTACG TGGACGACAC GCAGTTCGTG
            361 CGGTTCGACA GCGACGCCGC GAGCCAGAGG ATGGAGCCGC GGGCGCCGTG GATAGAGCAG
            421 GAGAGGCCTG AGTATTGGGA CCAGGAGACA CGGAATGTGA AGGCCCACTC ACAGATTGAC
            481 CGAGTGGACC TGGGGACCCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG TTCTCACACC
            541 ATCCAGATGA TGTATGGCTG CGACGTGGGG TCGGACGGGC GCTTCCTCCG CGGGTACCAG
            601 CAGGACGCCT ACGACGGCAA GGATTACATC GCCTTGAACG AGGACCTGCG CTCTTGGACC
            661 GCGGCGGACA TGGCGGCTCA GATCACCCAG CGCAAGTGGG AGGCGGCCCG TGTGGCGGAG
            721 CAGTTGAGAG CCTACCTGGA GGGCACGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC
            781 GGGAAGGAGA CGCTGCAGCG CACGGACCCC CCCAAGACGC ATATGACTCA CCACGCTGTC
            841 TCTGACCATG AGGCCACCCT GAGGTGCTGG GCCCTGAGCT TCTACCCTGC GGAGATCACA
            901 CTGACCTGGC AGCGGGATGG GGGAGGACCA GACCCAGGAC ACGGAGCTCG TGGAGACCAG
            961 GCCTGCAGGG GATGGAACCT TCCAGAAGTG GGCGTCTGTG GTGGTGCCTT CTGGACAGGG
           1021 AGCAGAGATA CACCTGCCAT GTGCAGCATG AGGGTCTCCC CCAAAGCCCC TCACCCTGAG
           1081 ATGGGAGCCC GTCTTTCCCC AGCCCACAAT CCCCAATCGA TGGGGGCCAT CAATTGGCTG
          1141 GGCCTAGCTC CTCTCTTTTT GGGAAGCC
          //
          Primer: BGH_rev
          Region: insert 3'
          Sequence file: RDB13689_A5Jgb.seq check
          >02872_13689_A5Jg_BGHrev_D09_12.ab1
              1 GAAAGGGGGG CCTCTAGACT CGAGCGATAT GCGGCCGCAC AAGGCAGCTG TCTCACACTT
             61 TACAAGCTGT GAGAGACATA TCAGAGCCCT GGGCACTGTC ACTGCTTGCA GCCTGAGAGT
            121 AGCTCCCTCC TTTTCTATCT GAGCTCTTCC TCCTCCACCT CACAGCAGCG ACCACAGCTC
            181 CAGCGAACAC AGCTCCAAAG AGAACTAGGC CAGCAATGAT GCCCACGATG GGGATGGTGG
            241 GCTGGGAAGA CGGCTCCCAT CTCAGGGTGA GGGGCTTGGG GAGACCCTCA TGCTGCACAT
            301 GGCAGGTGTA TCTCTGCTCC TGTCCAGAAG GCACCACCAC AGACGCCCAC TTCTGGAAGG
            361 TTCCATCCCC TGCAGGCCTG GTCTCCACGA GCTCCGTGTC CTGGGTCTGG TCCTCCCCAT
            421 CCCGCTGCCA GGTCAGTGTG ATCTCCGCAG GGTAGAAGCT CAGGGCCCAG CACCTCAGGG
            481 TGGCCTCATG GTCAGAGACA GCGTGGTGAG TCATATGCGT CTTGGGGGGG TCCGTGCGCT
            541 GCAGCGTCTC CTTCCCGTTC TCCAGGTATC TGCGGAGCCA CTCCACGCAC GTGCCCTCCA
            601 GGTAGGCTCT CAACTGCTCC GCCACACGGG CCGCCTCCCA CTTGCGCTGG GTGATCTGAG
            661 CCGCCATGTC CGCCGCGGTC CAAGAGCGCA GGTCCTCGTT CAAGGCGATG TAATCCTTGC
            721 CGTCGTAGGC GTCCTGCTGG TACCCGCGGA GGAAGCGCCC GTCCGACCCC ACGTCGCAGC
            781 CATACATCAT CTGGATGGTG TGAGAACCGG CCTCGCTCTG GTTGTAGTAG CCGCGCAGGG
            841 TCCCCAGGTC CACTCGGTCA ATCTGTGAGT GGGCCTTCAC ATTCCGTGTC TCCTGGTCCC
            901 AATACTCAGG CCTCTCCTGC TCTATCCACG GCGCTCTGCG GCTCTCATCC TCTGGCTCGC
            961 GGGCGTCGCT GTCGAACCCG CACGAACTGC GTGTCGTCCA CGTAGCCTCA CGGCGATGAA
           1021 GCGGGGCTTC CCCGCGGGCC GGGGCCCGGG GACACGGATG TGGGTGGAAA TACCCTTCAT
           1081 GGAGGTGGCA GCCTGACTCT AAGATCTGGG GCTCATGGTT CCTAAGGGGA CTCTCTCTCT
          1141 AAGAGGTTAT AGACG
          //
          Primer: SV40pro_F_v2
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB13689_A5Jgc.seq check
          >02872_13689_A5Jg_SV40pro_F_V2_F04_16.ab1
              1 GGCCTTTTTG TAGCTAGGCT TTTGCAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
             61 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA
            121 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
            181 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
            241 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG
            301 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
            361 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
            421 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
            481 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA
            541 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA
            601 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC
            661 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT
            721 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT
            781 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC
            841 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG
            901 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG
            961 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
           1021 TCCAGCGCGG GGGATCTCAT GCTGGGAGTT CTTCGCCCCA CCCCCACTTT GTTTATTGCC
           1081 AGCCTTTATA AATGCTACAA TAAGCAATAG CATCACAAAT TTTCCACCAT TTAAAGGCCA
          1141 A
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl