RDB02872 - Human HLA-A*31:01:02 cDNA

Resource data sheet
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Human HLA-A*31:01:02 cDNA

Human HLA-A*31:01:02 cDNA from human EBV-transformed B cell line cDNA


Catalog number	RDB02872
Resource name	Human HLA-A*31:01:02 cDNA
Alternative name	HLA-A*31012
Clone info.	Human HLA-A*31:01:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human major histocompatibility complex, class I, A cDNA
Depositor	Akatsuka, Yoshiki \|
Other clones in our bank	human HLA-A (NCBI Gene 3105) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB02872	Human HLA-A*31:01:02 cDNA	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB02872z.seq from Depositor
Electronic file	Map RDB02872.gif from Depositor

Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13689_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward	RDB13689_A5Jga.seq
>02872_13689_A5Jg_CMV-Forward_D04_10.ab1 1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGATTC TCCCCAGACG 181 CCGAGGATGG CCGTCATGGC GCCCCGAACC CTCCTCCTGC TACTCTTGGG GGCCCTGGCC 241 CTGACCCAGA CCTGGGCGGG CTCCCACTCC ATGAGGTATT TCACCACATC CGTGTCCCGG 301 CCCGGCCGCG GGGAGCCCCG CTTCATCGCC GTGGGCTACG TGGACGACAC GCAGTTCGTG 361 CGGTTCGACA GCGACGCCGC GAGCCAGAGG ATGGAGCCGC GGGCGCCGTG GATAGAGCAG 421 GAGAGGCCTG AGTATTGGGA CCAGGAGACA CGGAATGTGA AGGCCCACTC ACAGATTGAC 481 CGAGTGGACC TGGGGACCCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG TTCTCACACC 541 ATCCAGATGA TGTATGGCTG CGACGTGGGG TCGGACGGGC GCTTCCTCCG CGGGTACCAG 601 CAGGACGCCT ACGACGGCAA GGATTACATC GCCTTGAACG AGGACCTGCG CTCTTGGACC 661 GCGGCGGACA TGGCGGCTCA GATCACCCAG CGCAAGTGGG AGGCGGCCCG TGTGGCGGAG 721 CAGTTGAGAG CCTACCTGGA GGGCACGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC 781 GGGAAGGAGA CGCTGCAGCG CACGGACCCC CCCAAGACGC ATATGACTCA CCACGCTGTC 841 TCTGACCATG AGGCCACCCT GAGGTGCTGG GCCCTGAGCT TCTACCCTGC GGAGATCACA 901 CTGACCTGGC AGCGGGATGG GGGAGGACCA GACCCAGGAC ACGGAGCTCG TGGAGACCAG 961 GCCTGCAGGG GATGGAACCT TCCAGAAGTG GGCGTCTGTG GTGGTGCCTT CTGGACAGGG 1021 AGCAGAGATA CACCTGCCAT GTGCAGCATG AGGGTCTCCC CCAAAGCCCC TCACCCTGAG 1081 ATGGGAGCCC GTCTTTCCCC AGCCCACAAT CCCCAATCGA TGGGGGCCAT CAATTGGCTG 1141 GGCCTAGCTC CTCTCTTTTT GGGAAGCC //
Primer: BGH_rev	RDB13689_A5Jgb.seq
>02872_13689_A5Jg_BGHrev_D09_12.ab1 1 GAAAGGGGGG CCTCTAGACT CGAGCGATAT GCGGCCGCAC AAGGCAGCTG TCTCACACTT 61 TACAAGCTGT GAGAGACATA TCAGAGCCCT GGGCACTGTC ACTGCTTGCA GCCTGAGAGT 121 AGCTCCCTCC TTTTCTATCT GAGCTCTTCC TCCTCCACCT CACAGCAGCG ACCACAGCTC 181 CAGCGAACAC AGCTCCAAAG AGAACTAGGC CAGCAATGAT GCCCACGATG GGGATGGTGG 241 GCTGGGAAGA CGGCTCCCAT CTCAGGGTGA GGGGCTTGGG GAGACCCTCA TGCTGCACAT 301 GGCAGGTGTA TCTCTGCTCC TGTCCAGAAG GCACCACCAC AGACGCCCAC TTCTGGAAGG 361 TTCCATCCCC TGCAGGCCTG GTCTCCACGA GCTCCGTGTC CTGGGTCTGG TCCTCCCCAT 421 CCCGCTGCCA GGTCAGTGTG ATCTCCGCAG GGTAGAAGCT CAGGGCCCAG CACCTCAGGG 481 TGGCCTCATG GTCAGAGACA GCGTGGTGAG TCATATGCGT CTTGGGGGGG TCCGTGCGCT 541 GCAGCGTCTC CTTCCCGTTC TCCAGGTATC TGCGGAGCCA CTCCACGCAC GTGCCCTCCA 601 GGTAGGCTCT CAACTGCTCC GCCACACGGG CCGCCTCCCA CTTGCGCTGG GTGATCTGAG 661 CCGCCATGTC CGCCGCGGTC CAAGAGCGCA GGTCCTCGTT CAAGGCGATG TAATCCTTGC 721 CGTCGTAGGC GTCCTGCTGG TACCCGCGGA GGAAGCGCCC GTCCGACCCC ACGTCGCAGC 781 CATACATCAT CTGGATGGTG TGAGAACCGG CCTCGCTCTG GTTGTAGTAG CCGCGCAGGG 841 TCCCCAGGTC CACTCGGTCA ATCTGTGAGT GGGCCTTCAC ATTCCGTGTC TCCTGGTCCC 901 AATACTCAGG CCTCTCCTGC TCTATCCACG GCGCTCTGCG GCTCTCATCC TCTGGCTCGC 961 GGGCGTCGCT GTCGAACCCG CACGAACTGC GTGTCGTCCA CGTAGCCTCA CGGCGATGAA 1021 GCGGGGCTTC CCCGCGGGCC GGGGCCCGGG GACACGGATG TGGGTGGAAA TACCCTTCAT 1081 GGAGGTGGCA GCCTGACTCT AAGATCTGGG GCTCATGGTT CCTAAGGGGA CTCTCTCTCT 1141 AAGAGGTTAT AGACG //
Primer: SV40pro_F_V2	RDB13689_A5Jgc.seq
>02872_13689_A5Jg_SV40pro_F_V2_F04_16.ab1 1 GGCCTTTTTG TAGCTAGGCT TTTGCAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 61 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 121 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 181 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 241 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 301 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 361 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 421 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 481 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 541 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 601 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 661 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 721 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 781 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 841 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 901 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 961 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 1021 TCCAGCGCGG GGGATCTCAT GCTGGGAGTT CTTCGCCCCA CCCCCACTTT GTTTATTGCC 1081 AGCCTTTATA AATGCTACAA TAAGCAATAG CATCACAAAT TTTCCACCAT TTAAAGGCCA 1141 A //

Please visit Sequencing and PCR primers for primer information.

References

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl