Resource data sheet
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Human HLA-A*31:01:02 cDNA

Human HLA-A*31:01:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02872
Resource name Human HLA-A*31:01:02 cDNA
Alternative name HLA-A*31012
Clone info. Human HLA-A*31:01:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02872 Human HLA-A*31:01:02 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02872z.seq from Depositor
Electronic file Map RDB02872.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13689_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13689_A5Jga.seq
>02872_13689_A5Jg_CMV-Forward_D04_10.ab1
    1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGATTC TCCCCAGACG
  181 CCGAGGATGG CCGTCATGGC GCCCCGAACC CTCCTCCTGC TACTCTTGGG GGCCCTGGCC
  241 CTGACCCAGA CCTGGGCGGG CTCCCACTCC ATGAGGTATT TCACCACATC CGTGTCCCGG
  301 CCCGGCCGCG GGGAGCCCCG CTTCATCGCC GTGGGCTACG TGGACGACAC GCAGTTCGTG
  361 CGGTTCGACA GCGACGCCGC GAGCCAGAGG ATGGAGCCGC GGGCGCCGTG GATAGAGCAG
  421 GAGAGGCCTG AGTATTGGGA CCAGGAGACA CGGAATGTGA AGGCCCACTC ACAGATTGAC
  481 CGAGTGGACC TGGGGACCCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG TTCTCACACC
  541 ATCCAGATGA TGTATGGCTG CGACGTGGGG TCGGACGGGC GCTTCCTCCG CGGGTACCAG
  601 CAGGACGCCT ACGACGGCAA GGATTACATC GCCTTGAACG AGGACCTGCG CTCTTGGACC
  661 GCGGCGGACA TGGCGGCTCA GATCACCCAG CGCAAGTGGG AGGCGGCCCG TGTGGCGGAG
  721 CAGTTGAGAG CCTACCTGGA GGGCACGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC
  781 GGGAAGGAGA CGCTGCAGCG CACGGACCCC CCCAAGACGC ATATGACTCA CCACGCTGTC
  841 TCTGACCATG AGGCCACCCT GAGGTGCTGG GCCCTGAGCT TCTACCCTGC GGAGATCACA
  901 CTGACCTGGC AGCGGGATGG GGGAGGACCA GACCCAGGAC ACGGAGCTCG TGGAGACCAG
  961 GCCTGCAGGG GATGGAACCT TCCAGAAGTG GGCGTCTGTG GTGGTGCCTT CTGGACAGGG
 1021 AGCAGAGATA CACCTGCCAT GTGCAGCATG AGGGTCTCCC CCAAAGCCCC TCACCCTGAG
 1081 ATGGGAGCCC GTCTTTCCCC AGCCCACAAT CCCCAATCGA TGGGGGCCAT CAATTGGCTG
1141 GGCCTAGCTC CTCTCTTTTT GGGAAGCC
//
Primer: BGH_rev RDB13689_A5Jgb.seq
>02872_13689_A5Jg_BGHrev_D09_12.ab1
    1 GAAAGGGGGG CCTCTAGACT CGAGCGATAT GCGGCCGCAC AAGGCAGCTG TCTCACACTT
   61 TACAAGCTGT GAGAGACATA TCAGAGCCCT GGGCACTGTC ACTGCTTGCA GCCTGAGAGT
  121 AGCTCCCTCC TTTTCTATCT GAGCTCTTCC TCCTCCACCT CACAGCAGCG ACCACAGCTC
  181 CAGCGAACAC AGCTCCAAAG AGAACTAGGC CAGCAATGAT GCCCACGATG GGGATGGTGG
  241 GCTGGGAAGA CGGCTCCCAT CTCAGGGTGA GGGGCTTGGG GAGACCCTCA TGCTGCACAT
  301 GGCAGGTGTA TCTCTGCTCC TGTCCAGAAG GCACCACCAC AGACGCCCAC TTCTGGAAGG
  361 TTCCATCCCC TGCAGGCCTG GTCTCCACGA GCTCCGTGTC CTGGGTCTGG TCCTCCCCAT
  421 CCCGCTGCCA GGTCAGTGTG ATCTCCGCAG GGTAGAAGCT CAGGGCCCAG CACCTCAGGG
  481 TGGCCTCATG GTCAGAGACA GCGTGGTGAG TCATATGCGT CTTGGGGGGG TCCGTGCGCT
  541 GCAGCGTCTC CTTCCCGTTC TCCAGGTATC TGCGGAGCCA CTCCACGCAC GTGCCCTCCA
  601 GGTAGGCTCT CAACTGCTCC GCCACACGGG CCGCCTCCCA CTTGCGCTGG GTGATCTGAG
  661 CCGCCATGTC CGCCGCGGTC CAAGAGCGCA GGTCCTCGTT CAAGGCGATG TAATCCTTGC
  721 CGTCGTAGGC GTCCTGCTGG TACCCGCGGA GGAAGCGCCC GTCCGACCCC ACGTCGCAGC
  781 CATACATCAT CTGGATGGTG TGAGAACCGG CCTCGCTCTG GTTGTAGTAG CCGCGCAGGG
  841 TCCCCAGGTC CACTCGGTCA ATCTGTGAGT GGGCCTTCAC ATTCCGTGTC TCCTGGTCCC
  901 AATACTCAGG CCTCTCCTGC TCTATCCACG GCGCTCTGCG GCTCTCATCC TCTGGCTCGC
  961 GGGCGTCGCT GTCGAACCCG CACGAACTGC GTGTCGTCCA CGTAGCCTCA CGGCGATGAA
 1021 GCGGGGCTTC CCCGCGGGCC GGGGCCCGGG GACACGGATG TGGGTGGAAA TACCCTTCAT
 1081 GGAGGTGGCA GCCTGACTCT AAGATCTGGG GCTCATGGTT CCTAAGGGGA CTCTCTCTCT
1141 AAGAGGTTAT AGACG
//
Primer: SV40pro_F_V2 RDB13689_A5Jgc.seq
>02872_13689_A5Jg_SV40pro_F_V2_F04_16.ab1
    1 GGCCTTTTTG TAGCTAGGCT TTTGCAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
   61 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA
  121 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
  181 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
  241 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG
  301 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
  361 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
  421 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
  481 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA
  541 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA
  601 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC
  661 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT
  721 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT
  781 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC
  841 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG
  901 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG
  961 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
 1021 TCCAGCGCGG GGGATCTCAT GCTGGGAGTT CTTCGCCCCA CCCCCACTTT GTTTATTGCC
 1081 AGCCTTTATA AATGCTACAA TAAGCAATAG CATCACAAAT TTTCCACCAT TTAAAGGCCA
1141 A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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