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Human HLA-A*24:02:01 cDNA

Human HLA-A*24:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02871
Resource name Human HLA-A*24:02:01 cDNA
Alternative name HLA-A*24021
Clone info. Human HLA-A*24:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02871 Human HLA-A*24:02:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02871z.seq
Electronic file Map RDB02871.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18217_B0Eqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB18217_B0Eqa.seq check
>02871_18217_B0Eq_3_CMV-Forward_A08_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGATT CTCCCCAGAC
  181 GCCGAGGATG GCCGTCATGG CGCCCCGAAC CCTCGTCCTG CTACTCTCGG GGGCCCTGGC
  241 CCTGACCCAG ACCTGGGCAG GCTCCCACTC CATGAGGTAT TTCTCCACAT CCGTGTCCCG
  301 GCCCGGCCGC GGGGAGCCCC GCTTCATCGC CGTGGGCTAC GTGGACGACA CGCAGTTCGT
  361 GCGGTTCGAC AGCGACGCCG CGAGCCAGAG GATGGAGCCG CGGGCGCCGT GGATAGAGCA
  421 GGAGGGGCCG GAGTATTGGG ACGAGGAGAC AGGGAAAGTG AAGGCCCACT CACAGACTGA
  481 CCGAGAGAAC CTGCGGATCG CGCTCCGCTA CTACAACCAG AGCGAGGCCG GTTCTCACAC
  541 CCTCCAGATG ATGTTTGGCT GCGACGTGGG GTCGGACGGG CGCTTCCTCC GCGGGTACCA
  601 CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAA GAGGANCTGC GCTCTTGGAC
  661 CGCGGCGGAC ATGGCGGCTC AGATCACCAA GCGCAAGTGG GAGGCGGCCC ATGTGGCGGA
  721 NCAGCAGAGA GCCTACCTGG ANGGCACGTG CGTGGACGGG CTCCGCAGAT ACCTGGANAA
  781 CGGGAANGAG ACGCTGCAGC GCACGGNCCC CCCCCNAGAN ACATATGANC CACCNCCCNN
  841 TCTCTGANNN TGANGNCACT CNNANATGNT GGGCCCNGGG CTTCNACCCN GCNNNNATNA
901 CNNNNNNNNN N
//
Primer: BGH_rev2
Region: BGH p(A), insert 3'
Sequence file: RDB18217_B0Eqb.seq check
>02871_18217_B0Eq_3_BGH_rev2_B08_02_ABI08.ab1
    1 NNNNNNNNNN NNGGNNGNNG GGCNNNCNAC AGATGGCTGG CAACTAGAAG GCACAGTCGA
   61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCACAA GGCAGCTGTC
  121 TCACACTTTA CAAGCTGTGA GAGACACATC AGAGCCCTGG GCACTGTCAC TGCTTGCAGC
  181 CTGAGAGTAG CTCCCTCCTT TTCTATCTGA GCTGTTCCTC CTCCACATCA CAGCAGCGAC
  241 CACAGCTCCA GTGATCACAG CTCCAAGGAG AACCAGGCCA GCAATGATGC CCACGATGGG
  301 GACGGTGGGC TGGGAAGATG GCTCCCATCT CAGGGTGAGG GGCTTGGGCA GACCCTCATG
  361 CTGCACATGG CAGGTGTATC TCTGCTCCTC TCCAGAAGGT ACCACCACAG CTGCCCACTT
  421 CTGGAAGGTT CCATCCCCTG CAGGCCTGGT CTCCACAAGC TCCGTGTCCT GGGTCTGGTC
  481 CTCCCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG TAGAAGCCCA GGGCCCAGCA
  541 TCTCAGAGTG GCCTCATGGT CAGAGATGGG GTGGTGGGTC ATATGTGTCT TGGGGGGGTC
  601 CGTGCGCTGC AGCGTCTCCT TCCCGTTCTC CAGGTATCTG CGGAGCCCGT CCACGCACGT
  661 GCCCTCCAGG TANGCTCTCT GCTGCTCCGC CACATGGGCC GCCTCCCACT TGCGCTTGGT
  721 GATCTGAGCC GCCATGTCCG CCGCGGTCCN AGANCGCANG TCCTCTTTCA NGGCGATGTA
  781 ATCCTTGCCG TCNTNNNGTA CTNGTGGTAC CNGCGGANGA NNCGCCCGTN CGANCCCACG
  841 TCGCANCCAA ACATCATCTG NANGGNGTGA NANCCNGCCT CGNTNNNGNT GNANTANCGG
901 ANCGCGATNC NNNNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, NeoR
Sequence file: RDB18217_B0Eqc.seq check
>02871_18217_B0Eq_3_SV40pro_ori_F_C08_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGC CGCCNCTGCC TCTGANCTNT TCNNGAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
  121 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA
  181 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
  241 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
  301 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG
  361 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
  421 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
  481 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
  541 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA
  601 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA
  661 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGNNG ATCTCGTCGT GACCCATGGC
  721 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACNGT
  781 GGCCGGCTGG NNGTGGCGGA NCGCTATCAN GANATAGCGT TGGCTANCCG TGATATNGCT
  841 GNANANCTTG GCGGCNAANG GGCTGNNCGC TTCCNCGNGC TTNACNGTAT CGCCGCTCCC
901 GATTCNCANC NNNTCGCCNT CNATCGNCNT NNTNNNNNAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.06.25

GNP_filter3_RDBDEP_html_200316.pl