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Human HLA-A*24:02:01 cDNA

Human HLA-A*24:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02871
Resource name Human HLA-A*24:02:01 cDNA
Alternative name HLA-A*24021
Clone info. Human HLA-A*24:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB02871 Human HLA-A*24:02:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02871z.seq
          Remarks, protocol and/or map (gif) RDB02871.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18217_B0Eqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB18217_B0Eqa.seq check
          >02871_18217_B0Eq_3_CMV-Forward_A08_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNTGNN GCNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGATT CTCCCCAGAC
            181 GCCGAGGATG GCCGTCATGG CGCCCCGAAC CCTCGTCCTG CTACTCTCGG GGGCCCTGGC
            241 CCTGACCCAG ACCTGGGCAG GCTCCCACTC CATGAGGTAT TTCTCCACAT CCGTGTCCCG
            301 GCCCGGCCGC GGGGAGCCCC GCTTCATCGC CGTGGGCTAC GTGGACGACA CGCAGTTCGT
            361 GCGGTTCGAC AGCGACGCCG CGAGCCAGAG GATGGAGCCG CGGGCGCCGT GGATAGAGCA
            421 GGAGGGGCCG GAGTATTGGG ACGAGGAGAC AGGGAAAGTG AAGGCCCACT CACAGACTGA
            481 CCGAGAGAAC CTGCGGATCG CGCTCCGCTA CTACAACCAG AGCGAGGCCG GTTCTCACAC
            541 CCTCCAGATG ATGTTTGGCT GCGACGTGGG GTCGGACGGG CGCTTCCTCC GCGGGTACCA
            601 CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAA GAGGANCTGC GCTCTTGGAC
            661 CGCGGCGGAC ATGGCGGCTC AGATCACCAA GCGCAAGTGG GAGGCGGCCC ATGTGGCGGA
            721 NCAGCAGAGA GCCTACCTGG ANGGCACGTG CGTGGACGGG CTCCGCAGAT ACCTGGANAA
            781 CGGGAANGAG ACGCTGCAGC GCACGGNCCC CCCCCNAGAN ACATATGANC CACCNCCCNN
            841 TCTCTGANNN TGANGNCACT CNNANATGNT GGGCCCNGGG CTTCNACCCN GCNNNNATNA
          901 CNNNNNNNNN N
          //
          Primer: BGH_rev2
          Sequence file: RDB18217_B0Eqb.seq check
          >02871_18217_B0Eq_3_BGH_rev2_B08_02_ABI08.ab1
              1 NNNNNNNNNN NNGGNNGNNG GGCNNNCNAC AGATGGCTGG CAACTAGAAG GCACAGTCGA
             61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCACAA GGCAGCTGTC
            121 TCACACTTTA CAAGCTGTGA GAGACACATC AGAGCCCTGG GCACTGTCAC TGCTTGCAGC
            181 CTGAGAGTAG CTCCCTCCTT TTCTATCTGA GCTGTTCCTC CTCCACATCA CAGCAGCGAC
            241 CACAGCTCCA GTGATCACAG CTCCAAGGAG AACCAGGCCA GCAATGATGC CCACGATGGG
            301 GACGGTGGGC TGGGAAGATG GCTCCCATCT CAGGGTGAGG GGCTTGGGCA GACCCTCATG
            361 CTGCACATGG CAGGTGTATC TCTGCTCCTC TCCAGAAGGT ACCACCACAG CTGCCCACTT
            421 CTGGAAGGTT CCATCCCCTG CAGGCCTGGT CTCCACAAGC TCCGTGTCCT GGGTCTGGTC
            481 CTCCCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG TAGAAGCCCA GGGCCCAGCA
            541 TCTCAGAGTG GCCTCATGGT CAGAGATGGG GTGGTGGGTC ATATGTGTCT TGGGGGGGTC
            601 CGTGCGCTGC AGCGTCTCCT TCCCGTTCTC CAGGTATCTG CGGAGCCCGT CCACGCACGT
            661 GCCCTCCAGG TANGCTCTCT GCTGCTCCGC CACATGGGCC GCCTCCCACT TGCGCTTGGT
            721 GATCTGAGCC GCCATGTCCG CCGCGGTCCN AGANCGCANG TCCTCTTTCA NGGCGATGTA
            781 ATCCTTGCCG TCNTNNNGTA CTNGTGGTAC CNGCGGANGA NNCGCCCGTN CGANCCCACG
            841 TCGCANCCAA ACATCATCTG NANGGNGTGA NANCCNGCCT CGNTNNNGNT GNANTANCGG
          901 ANCGCGATNC NNNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Sequence file: RDB18217_B0Eqc.seq check
          >02871_18217_B0Eq_3_SV40pro_ori_F_C08_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNGC CGCCNCTGCC TCTGANCTNT TCNNGAAGTA GTGAGGAGGC
             61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA
            121 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA
            181 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
            241 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
            301 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG
            361 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
            421 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
            481 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
            541 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA
            601 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA
            661 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGNNG ATCTCGTCGT GACCCATGGC
            721 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACNGT
            781 GGCCGGCTGG NNGTGGCGGA NCGCTATCAN GANATAGCGT TGGCTANCCG TGATATNGCT
            841 GNANANCTTG GCGGCNAANG GGCTGNNCGC TTCCNCGNGC TTNACNGTAT CGCCGCTCCC
          901 GATTCNCANC NNNTCGCCNT CNATCGNCNT NNTNNNNNAN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl