Resource data sheet
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Human HLA-A*24:02:01 cDNA

Human HLA-A*24:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02871
Resource name Human HLA-A*24:02:01 cDNA
Alternative name HLA-A*24021
Clone info. Human HLA-A*24:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02871 Human HLA-A*24:02:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02871z.seq from Depositor
Electronic file Map RDB02871.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13016_A5Jkp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13016_A5Jka.seq
>02871_13016_A5Jk_CMV-Forward_C08_08.ab1
    1 CGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTGATTCT CCCCAGACGC
  181 CGAGGATGGC CGTCATGGCG CCCCGAACCC TCGTCCTGCT ACTCTCGGGG GCCCTGGCCC
  241 TGACCCAGAC CTGGGCAGGC TCCCACTCCA TGAGGTATTT CTCCACATCC GTGTCCCGGC
  301 CCGGCCGCGG GGAGCCCCGC TTCATCGCCG TGGGCTACGT GGACGACACG CAGTTCGTGC
  361 GGTTCGACAG CGACGCCGCG AGCCAGAGGA TGGAGCCGCG GGCGCCGTGG ATAGAGCAGG
  421 AGGGGCCGGA GTATTGGGAC GAGGAGACAG GGAAAGTGAA GGCCCACTCA CAGACTGACC
  481 GAGAGAACCT GCGGATCGCG CTCCGCTACT ACAACCAGAG CGAGGCCGGT TCTCACACCC
  541 TCCAGATGAT GTTTGGCTGC GACGTGGGGT CGGACGGGCG CTTCCTCCGC GGGTACCACC
  601 AGTACGCCTA CGACGGCAAG GATTACATCG CCCTGAAAGA GGACCTGCGC TCTTGGACCG
  661 CGGCGGACAT GGCGGCTCAG ATCACCAAGC GCAAGTGGGA GGCGGCCCAT GTGGCGGAGC
  721 AGCAGAGAGC CTACCTGGAG GGCACGTGCG TGGACGGGCT CCGCAGATAC CTGGAGAACG
  781 GGAAGGAGAC GCTGCAGCGC ACGGACCCCC CCAAGACACA TATGACCCAC CACCCCATCT
  841 CTGACCATGA GGCCACTCTG AGATGCTGGG CCCTGGGCTT CTACCCTGCG GAGATCACAC
  901 TGACCTGGCA GCGGGATGGG GGAGGACCAG ACCCAGGACA CGGAGCTTGT GGAGACCAGG
  961 CCTGCACGGG ATGGAAACCT TCCAGAAGTG GGCAGCTGTG GTGGTACCTT CTGGAGAGGA
 1021 GCAGAGATAC ACCCTGCCAT GTGCAGCATG AGGTCTGCCC AAGCCCCCTC ACCCTGAGAT
1081 GGAAGTCAAT CCTTCCCAGC CTCACTCGAT CCTCTCAATT CGGGTGGGGC CAATC
//
Primer: BGH_rev RDB13016_A5Jkb.seq
>02871_13016_A5Jk_BGHrev_D08_11.ab1
    1 GATTACGGGC TCTAGACTCG AGCGGCCGCA CAAGGCAGCT GTCTCACACT TTACAAGCTG
   61 TGAGAGACAC ATCAGAGCCC TGGGCACTGT CACTGCTTGC AGCCTGAGAG TAGCTCCCTC
  121 CTTTTCTATC TGAGCTGTTC CTCCTCCACA TCACAGCAGC GACCACAGCT CCAGTGATCA
  181 CAGCTCCAAG GAGAACCAGG CCAGCAATGA TGCCCACGAT GGGGACGGTG GGCTGGGAAG
  241 ATGGCTCCCA TCTCAGGGTG AGGGGCTTGG GCAGACCCTC ATGCTGCACA TGGCAGGTGT
  301 ATCTCTGCTC CTCTCCAGAA GGTACCACCA CAGCTGCCCA CTTCTGGAAG GTTCCATCCC
  361 CTGCAGGCCT GGTCTCCACA AGCTCCGTGT CCTGGGTCTG GTCCTCCCCA TCCCGCTGCC
  421 AGGTCAGTGT GATCTCCGCA GGGTAGAAGC CCAGGGCCCA GCATCTCAGA GTGGCCTCAT
  481 GGTCAGAGAT GGGGTGGTGG GTCATATGTG TCTTGGGGGG GTCCGTGCGC TGCAGCGTCT
  541 CCTTCCCGTT CTCCAGGTAT CTGCGGAGCC CGTCCACGCA CGTGCCCTCC AGGTAGGCTC
  601 TCTGCTGCTC CGCCACATGG GCCGCCTCCC ACTTGCGCTT GGTGATCTGA GCCGCCATGT
  661 CCGCCGCGGT CCAAGAGCGC AGGTCCTCTT TCAGGGCGAT GTAATCCTTG CCGTCGTAGG
  721 CGTACTGGTG GTACCCGCGG AGGAAGCGCC CGTCCGACCC CACGTCGCAG CCAAACATCA
  781 TCTGGAGGGT GTGAGAACCG GCCTCGCTCT GGTTGTAGTA GCGGAGCGCG ATCCGCAGGT
  841 TCTCTCGGTC AGTCTGTGAG TGGGCCTTCA CTTTCCCTGT CTCCTCGTCC CAATACTCCG
  901 GCCCCTCCTG CTCTATCCAC GGCGCCCGCG GCTCCATCCT CTGGCTCGCG GCGTCGCTGT
  961 CGAACCGCAC GAAACTGCGT GTCGTCCACG TAGCCCACGG CGATGAAGCG GGGGCTCCCC
 1021 GCGGGCCGGG CCGGGACACG GATGTGGAGA AAATACCTCA TGGTAGTGGA GCCCTGCCAA
1081 GTTCTTGGAT TCAGATAAGG ACCCCCGGAG AGTAGCAGGA ACGTAATAGT
//
Primer: SV40pro_F_V2 RDB13016_A5Jkc.seq
>02871_13016_A5Jk_SV40pro_F_V2_E08_14.ab1
    1 GGGATGCCAT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGGAG CTTGTATATC
   61 CATTTTCGGA TCTGATCAAG AGAAAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG
  121 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA
  181 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT
  241 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG
  301 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA
  361 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA
  421 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT
  481 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC
  541 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC
  601 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT
  661 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT
  721 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG
  781 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT
  841 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC
  901 GGGACTCTGG GGGTTCGAAA TGACCGACCA AGCGACGCCC CAACCCTGCC ATCACGAGAT
  961 TTCGATTTCC ACCCGCCCGC CCTTTCTATG AAGGTTGGGC TTTCGGAAAT CGTTTTTCCC
 1021 GGGACGCCCG GCCTGGATGA TCCTCCCAGC GGCGGGGAAT CTCCATGCTG GAGGTTCATC
 1081 GCCCACCCCA ACTGTTTATG CCAGCCTATA TTGATTACAA TAAAAGGCAA TTAGCATTTC
1141 CACAAATTTT TCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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