Resource data sheet
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Human HLA-A*26:01 cDNA

Human HLA-A*26:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02870
Resource name Human HLA-A*26:01 cDNA
Alternative name HLA-A*2601
Clone info. Human HLA-A*26:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02870 Human HLA-A*26:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02870z.seq from Depositor
Electronic file Map RDB02870.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13688_A5Jgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13688_A5Jga.seq
>02870_13688_A5Jg_CMV-Forward_B09_06.ab1
    1 CTTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGATTC TCCCCAGACG
  181 CCGAGGATGG CCGTCATGGC GCCCCGAACC CTCGTCCTGC TACTCTCGGG GGCCCTGGCC
  241 CTGACCCAGA CCTGGGCGGG CTCCCACTCC ATGAGGTATT TCTACACCTC CGTGTCCCGG
  301 CCCGGCCGCG GGGAGCCCCG CTTCATCGCC GTGGGCTACG TGGACGACAC GCAGTTCGTG
  361 CGGTTCGACA GCGACGCCGC GAGCCAGAGG ATGGAGCCGC GGGCGCCGTG GATAGAGCAG
  421 GAGGGGCCGG AGTATTGGGA CCGGAACACA CGGAATGTGA AGGCCCACTC ACAGACTGAC
  481 CGAGCGAACC TGGGGACCCT GCGCGGCTAC TACAACCAGA GCGAGGACGG TTCTCACACC
  541 ATCCAGAGGA TGTATGGCTG CGACGTGGGG CCGGACGGGC GCTTCCTCCG CGGGTACCAG
  601 CAGGACGCTT ACGACGGCAA GGATTACATC GCCCTGAACG AGGACCTGCG CTCTTGGACC
  661 GCGGCGGACA TGGCGGCTCA GATCACCCAG CGCAAGTGGG AGACGGCCCA TGAGGCGGAG
  721 CAGTGGAGAG CCTACCTGGA GGGCCGGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC
  781 GGGAAGGAGA CGCTGCAGCG CACGGACGCC CCCAAGACGC ATATGACTCA CCACGCTGTC
  841 TCTGACCATG AGGCCACCCT GAGGTGCTGG GCCCTGAGCT TCTACCCTGC GGAGATCACA
  901 CTGACCTGGC AGCGGGATGG GGGAGGACCA GACCCAGGAC ACGGAGCTCG TGGAGACCAG
  961 GCCTGCAGGG GATGGGACCT TTCCAGAAGT GGGCGTCTGT GGTGGTGCCT TTCTGGACAG
 1021 GGAGCAGAGA TACACCTGCT CATGTGCAGC ATGAGGGATC TGCTCTAGCC CTCCTTCACT
 1081 CCTTGAGATG GGAGTGGTCT TCCTAGCTCA CCAATCCCTC TCAATCGATG GGGGCCATTC
1141 CAATTGTTC
//
Primer: BGH_rev RDB13688_A5Jgb.seq
>02870_13688_A5Jg_BGHrev_B04_04.ab1
    1 GATCGACTCT AGACTCGAGC GGCCGCACAA GGCAGCTGTC TCACACTTTA CAAGCTGTGA
   61 GAGACATATC AGAGCCCTGG GCACTGTCAC TGCTTGCAGC CTGAGAGTAG CTCCCTCCTT
  121 TTCTATCTGA GCTCTTCCTC CTCCACATCA CAGCAGCGAC CACAGCTCCA GCGATCACAG
  181 CTCCAAAGAG AACCAGGCCA GCAATGATGC CCACGATGGG GATGGTGGGC TGGGAAGACG
  241 GCTCCCATCT CAGGGTGAGG GGCTTGGGCA GACCCTCATG CTGCACATGG CAGGTGTATC
  301 TCTGCTCCTG TCCAGAAGGC ACCACCACAG ACGCCCACTT CTGGAAGGTC CCATCCCCTG
  361 CAGGCCTGGT CTCCACGAGC TCCGTGTCCT GGGTCTGGTC CTCCCCATCC CGCTGCCAGG
  421 TCAGTGTGAT CTCCGCAGGG TAGAAGCTCA GGGCCCAGCA CCTCAGGGTG GCCTCATGGT
  481 CAGAGACAGC GTGGTGAGTC ATATGCGTCT TGGGGGCGTC CGTGCGCTGC AGCGTCTCCT
  541 TCCCGTTCTC CAGGTATCTG CGGAGCCACT CCACGCACCG GCCCTCCAGG TAGGCTCTCC
  601 ACTGCTCCGC CTCATGGGCC GTCTCCCACT TGCGCTGGGT GATCTGAGCC GCCATGTCCG
  661 CCGCGGTCCA AGAGCGCAGG TCCTCGTTCA GGGCGATGTA ATCCTTGCCG TCGTAAGCGT
  721 CCTGCTGGTA CCCGCGGAGG AAGCGCCCGT CCGGCCCCAC GTCGCAGCCA TACATCCTCT
  781 GGATGGTGTG AGAACCGTCC TCGCTCTGGT TGTAGTAGCC GCGCAGGGTC CCCAGGTTCG
  841 CTCGGTCAGT CTGTGAGTGG GCCTTCACAT TCCGTGTGTT CCGGTCCCAA TACTCCGGCC
  901 CCTCCTGCTC TATCCACGGC GCCCGCGGCT CCATCCTCTG GCTCGCGGCG TCGCTGTCGA
  961 ACCGCACGAA CTGCGTGTCG TCCACGTAGC CCACGGCGAT GAAGCGGGGC TCCCCGCGGC
 1021 CGGGCCGGGA CACGGGAGGG TGTAGAAAAT ACCTCATGGG AGTGGAGCCC GCCCCAGATC
 1081 TGGGTCATGA CAGGACCCCC GAGAGTAGCC AGGACCGAAG GGATTCGGGG GCGCGCCCAA
1141 TTGAACCCG
//
Primer: SV40pro_F_V2 RDB13688_A5Jgc.seq
>02870_13688_A5Jg_SV40pro_F_V2_C09_09.ab1
    1 CGACCTTTTT TTTGGGAGCC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG TATATCCATT
   61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
  121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
  181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
  241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
  301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
  361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
  421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
  481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
  541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
  601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
  661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
  721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
  781 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC
  841 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA
  901 CTCTGGGGTT CGAAATGACC GACCAAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT
  961 TCCACCGCCG CCTTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTTCCCG GGACGCCGGC
 1021 TGGAATGAAT CCCTCCAGCG CGGGATCTCA TGCTGGAGGT TTCTTCCGCC AACCCCAAAC
 1081 TTGTTTATTG CCAAGCTATA ATGTACAAAT TAAGCAATTA GCCAATTCAC AAAATTTTTC
1141 ACCAGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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