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Human HLA-A*26:01 cDNA

Human HLA-A*26:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02870
Resource name Human HLA-A*26:01 cDNA
Alternative name HLA-A*2601
Clone info. Human HLA-A*26:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB02870 Human HLA-A*26:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02870z.seq
          Remarks, protocol and/or map (gif) RDB02870.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13688_A5Jgp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert
          Sequence file: RDB13688_A5Jga.seq check
          >02870_13688_A5Jg_CMV-Forward_B09_06.ab1
              1 CTTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGATTC TCCCCAGACG
            181 CCGAGGATGG CCGTCATGGC GCCCCGAACC CTCGTCCTGC TACTCTCGGG GGCCCTGGCC
            241 CTGACCCAGA CCTGGGCGGG CTCCCACTCC ATGAGGTATT TCTACACCTC CGTGTCCCGG
            301 CCCGGCCGCG GGGAGCCCCG CTTCATCGCC GTGGGCTACG TGGACGACAC GCAGTTCGTG
            361 CGGTTCGACA GCGACGCCGC GAGCCAGAGG ATGGAGCCGC GGGCGCCGTG GATAGAGCAG
            421 GAGGGGCCGG AGTATTGGGA CCGGAACACA CGGAATGTGA AGGCCCACTC ACAGACTGAC
            481 CGAGCGAACC TGGGGACCCT GCGCGGCTAC TACAACCAGA GCGAGGACGG TTCTCACACC
            541 ATCCAGAGGA TGTATGGCTG CGACGTGGGG CCGGACGGGC GCTTCCTCCG CGGGTACCAG
            601 CAGGACGCTT ACGACGGCAA GGATTACATC GCCCTGAACG AGGACCTGCG CTCTTGGACC
            661 GCGGCGGACA TGGCGGCTCA GATCACCCAG CGCAAGTGGG AGACGGCCCA TGAGGCGGAG
            721 CAGTGGAGAG CCTACCTGGA GGGCCGGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC
            781 GGGAAGGAGA CGCTGCAGCG CACGGACGCC CCCAAGACGC ATATGACTCA CCACGCTGTC
            841 TCTGACCATG AGGCCACCCT GAGGTGCTGG GCCCTGAGCT TCTACCCTGC GGAGATCACA
            901 CTGACCTGGC AGCGGGATGG GGGAGGACCA GACCCAGGAC ACGGAGCTCG TGGAGACCAG
            961 GCCTGCAGGG GATGGGACCT TTCCAGAAGT GGGCGTCTGT GGTGGTGCCT TTCTGGACAG
           1021 GGAGCAGAGA TACACCTGCT CATGTGCAGC ATGAGGGATC TGCTCTAGCC CTCCTTCACT
           1081 CCTTGAGATG GGAGTGGTCT TCCTAGCTCA CCAATCCCTC TCAATCGATG GGGGCCATTC
          1141 CAATTGTTC
          //
          Primer: BGH_rev
          Region: insert
          Sequence file: RDB13688_A5Jgb.seq check
          >02870_13688_A5Jg_BGHrev_B04_04.ab1
              1 GATCGACTCT AGACTCGAGC GGCCGCACAA GGCAGCTGTC TCACACTTTA CAAGCTGTGA
             61 GAGACATATC AGAGCCCTGG GCACTGTCAC TGCTTGCAGC CTGAGAGTAG CTCCCTCCTT
            121 TTCTATCTGA GCTCTTCCTC CTCCACATCA CAGCAGCGAC CACAGCTCCA GCGATCACAG
            181 CTCCAAAGAG AACCAGGCCA GCAATGATGC CCACGATGGG GATGGTGGGC TGGGAAGACG
            241 GCTCCCATCT CAGGGTGAGG GGCTTGGGCA GACCCTCATG CTGCACATGG CAGGTGTATC
            301 TCTGCTCCTG TCCAGAAGGC ACCACCACAG ACGCCCACTT CTGGAAGGTC CCATCCCCTG
            361 CAGGCCTGGT CTCCACGAGC TCCGTGTCCT GGGTCTGGTC CTCCCCATCC CGCTGCCAGG
            421 TCAGTGTGAT CTCCGCAGGG TAGAAGCTCA GGGCCCAGCA CCTCAGGGTG GCCTCATGGT
            481 CAGAGACAGC GTGGTGAGTC ATATGCGTCT TGGGGGCGTC CGTGCGCTGC AGCGTCTCCT
            541 TCCCGTTCTC CAGGTATCTG CGGAGCCACT CCACGCACCG GCCCTCCAGG TAGGCTCTCC
            601 ACTGCTCCGC CTCATGGGCC GTCTCCCACT TGCGCTGGGT GATCTGAGCC GCCATGTCCG
            661 CCGCGGTCCA AGAGCGCAGG TCCTCGTTCA GGGCGATGTA ATCCTTGCCG TCGTAAGCGT
            721 CCTGCTGGTA CCCGCGGAGG AAGCGCCCGT CCGGCCCCAC GTCGCAGCCA TACATCCTCT
            781 GGATGGTGTG AGAACCGTCC TCGCTCTGGT TGTAGTAGCC GCGCAGGGTC CCCAGGTTCG
            841 CTCGGTCAGT CTGTGAGTGG GCCTTCACAT TCCGTGTGTT CCGGTCCCAA TACTCCGGCC
            901 CCTCCTGCTC TATCCACGGC GCCCGCGGCT CCATCCTCTG GCTCGCGGCG TCGCTGTCGA
            961 ACCGCACGAA CTGCGTGTCG TCCACGTAGC CCACGGCGAT GAAGCGGGGC TCCCCGCGGC
           1021 CGGGCCGGGA CACGGGAGGG TGTAGAAAAT ACCTCATGGG AGTGGAGCCC GCCCCAGATC
           1081 TGGGTCATGA CAGGACCCCC GAGAGTAGCC AGGACCGAAG GGATTCGGGG GCGCGCCCAA
          1141 TTGAACCCG
          //
          Primer: SV40pro_F_V2
          Region: NeoR
          Sequence file: RDB13688_A5Jgc.seq check
          >02870_13688_A5Jg_SV40pro_F_V2_C09_09.ab1
              1 CGACCTTTTT TTTGGGAGCC TAGGCTTTTG CAAAAGCTCC CGGGAGCTTG TATATCCATT
             61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
            121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
            181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
            241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
            301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
            361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
            421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
            481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
            541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
            601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
            661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
            721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
            781 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC
            841 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA
            901 CTCTGGGGTT CGAAATGACC GACCAAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT
            961 TCCACCGCCG CCTTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTTCCCG GGACGCCGGC
           1021 TGGAATGAAT CCCTCCAGCG CGGGATCTCA TGCTGGAGGT TTCTTCCGCC AACCCCAAAC
           1081 TTGTTTATTG CCAAGCTATA ATGTACAAAT TAAGCAATTA GCCAATTCAC AAAATTTTTC
          1141 ACCAGCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl